+Open data
-Basic information
Entry | Database: PDB / ID: 1l0m | ||||||
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Title | Solution structure of Bacteriorhodopsin | ||||||
Components | Bacteriorhodopsin | ||||||
Keywords | PROTON TRANSPORT / Bacteriorhodopsin / alternative method for structure determination | ||||||
Function / homology | Function and homology information photoreceptor activity / phototransduction / proton transmembrane transport / monoatomic ion channel activity / plasma membrane Similarity search - Function | ||||||
Method | SOLUTION NMR / refined using molecular dynamics | ||||||
Authors | Katragadda, M. / Alderfer, J.L. / Yeagle, P.L. | ||||||
Citation | Journal: Biophys.J. / Year: 2001 Title: Assembly of a polytopic membrane protein structure from the solution structures of overlapping peptide fragments of bacteriorhodopsin. Authors: Katragadda, M. / Alderfer, J.L. / Yeagle, P.L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1l0m.cif.gz | 79.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1l0m.ent.gz | 63.9 KB | Display | PDB format |
PDBx/mmJSON format | 1l0m.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/l0/1l0m ftp://data.pdbj.org/pub/pdb/validation_reports/l0/1l0m | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 23256.477 Da / Num. of mol.: 1 / Mutation: part of helix G absent / Source method: obtained synthetically Details: The peptide, corresponding to different secondary structure elements, was chemically synthesized. The sequence of this peptide is naturally found in Halobacterium halobium. References: UniProt: P02945 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: chmeically synthesized peptides / Solvent system: DMSO |
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Sample conditions | pH: 6 / Pressure: 1 atm / Temperature: 293 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker AMX / Manufacturer: Bruker / Model: AMX / Field strength: 600 MHz |
-Processing
NMR software |
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Refinement | Method: refined using molecular dynamics / Software ordinal: 1 / Details: simulated annealing | |||||||||||||||
NMR ensemble | Conformers submitted total number: 1 |