SASDCB3: Methylated C-terminal ZBTB38 binding sequence (mCZ38BS)

基本情報

• 登録情報: データベース: SASBDB / ID: SASDCB3

試料

Methylated C-terminal ZBTB38 binding sequence (mCZ38BS)

• methylated C-terminal ZBTB38 binding sequence (DNA), mCZ38BS

• 引用: ジャーナル: J Mol Biol / 年: 2018; タイトル: The C-Terminal Zinc Fingers of ZBTB38 are Novel Selective Readers of DNA Methylation.; 著者: Amir Pozner / Nicholas O Hudson / Jill Trewhella / Tommy W Terooatea / Sven A Miller / Bethany A Buck-Koehntop / ; 要旨: Methyl-CpG binding proteins play an essential role in translating DNA methylation marks into a downstream transcriptional response, which has implications for both normal cell function as well as disease. Although for many of these proteins, a detailed mechanistic understanding for how this cellular process is mediated remains to be determined. ZBTB38 is an under-characterized member of the zinc finger (ZF) family of methyl-CpG binding proteins. Functional knowledge has been gained for its conserved methylated DNA binding N-terminal ZF region; however, a specific role for the C-terminal set of five ZFs remains to be elucidated. Here we demonstrate for the first time that a subset of the C-terminal ZBTB38 ZFs exhibit high-affinity DNA interactions and that preferential targeting of the consensus DNA site is methyl specific. Utilizing a hybrid approach, a model for the C-terminal ZBTB38 ZFs in complex with its cognate DNA target is proposed, providing insight into a possible novel mode of methylated DNA recognition. Furthermore, it is shown that the C-terminal ZFs of ZBTB38 can directly occupy promoters harboring the newly identified sequence motif in cell in a methyl-dependent manner and, depending on the gene context, contribute to modulating transcriptional response. Combined, these findings provide evidence for a key and novel physiological function for the C-terminal ZF domain of ZBTB38.

登録者

• Jill Trewhella (School of Molecular Bioscience, University of Sydney., Sydney, New South Wales 2006, Australia)

モデル

• モデル #1236: ; タイプ: dummy / ダミー原子の半径: 2.40 A / コメント: methylated C-terminal ZBTB38 binding sequence / カイ2乗値: 1.121 / P-value: 0.848600; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: Methylated C-terminal ZBTB38 binding sequence (mCZ38BS); 試料濃度: 4.2 mg/ml
• バッファ: 名称: 10 mM Tris, 1 mM tris(2-carboxy-ethyl)phosphine (TCEP), 0.05% NaN3, 10% D2O; pH: 6.8 ; コメント: Solvent blank taken from flow through in concentration step

要素 #666

名称: mCZ38BS / タイプ: DNA / 記述: methylated C-terminal ZBTB38 binding sequence / 分子量: 16.705 / 分子数: 1
配列:

GCACTCATCG GCGCAGATCA GCTAGCCGGC TAGCTGATCT GCGCCGATGA GTGC

実験情報

• ビーム: 設備名称: Department of Chemistry, University of Utah Anton Paar SAXSess; 地域: Salt Lake City, UT / 国: USA / 線源: X-ray in houseX線 / 波長: 0.154 Å / スペクトロメータ・検出器間距離: 0.2612 mm
• 検出器: 名称: Dectris Mythen 1K / タイプ: 1D diode array detector / Pixsize x: 50 mm

スキャン

タイトル: methylated C-terminal ZBTB38 binding sequence (mCZ38BS)
測定日: 2016年10月27日 / セル温度: 22 °C / 照射時間: 30 sec. / フレーム数: 120 / 単位: 1/nm /

	Min	Max
Q	0.1	3.7176

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 475 /

	Min	Max
Q	0.1	3.71761
P(R) point	1	475
R	0	9.2

結果

カーブのタイプ: single_conc

	実験値	Standard	Porod
分子量	16.632 kDa	16.924 kDa	15.3 kDa
体積	-	-	23 nm3

	P(R)	P(R) error	Guinier	Guinier error
前方散乱 I0	0.1143	0.00149	0.1135	0.0004
慣性半径, Rg	2.62 nm	0.06	2.51 nm	0.03

	Min	Max
D	-	9.2
Guinier point	1	52