+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 6hka | ||||||
---|---|---|---|---|---|---|---|
タイトル | The solution structure of the micelle-associated FATC domain of the human protein kinase ataxia telangiectasia mutated (ATM) | ||||||
要素 | Immunoglobulin G-binding protein G,Serine-protein kinase ATM | ||||||
キーワード | TRANSFERASE (転移酵素) / Ataxia telangiectasia mutated (ATM) / FATC domain / phosphatidylinositol-3-kinase-related kinase (PIKKs) / ser/thr kinase / membrane binding | ||||||
機能・相同性 | 機能・相同性情報 positive regulation of DNA catabolic process / establishment of RNA localization to telomere / positive regulation of telomerase catalytic core complex assembly / cellular response to nitrosative stress / positive regulation of DNA damage response, signal transduction by p53 class mediator / establishment of protein-containing complex localization to telomere / regulation of microglial cell activation / negative regulation of telomere capping / Sensing of DNA Double Strand Breaks / positive regulation of telomere maintenance via telomere lengthening ...positive regulation of DNA catabolic process / establishment of RNA localization to telomere / positive regulation of telomerase catalytic core complex assembly / cellular response to nitrosative stress / positive regulation of DNA damage response, signal transduction by p53 class mediator / establishment of protein-containing complex localization to telomere / regulation of microglial cell activation / negative regulation of telomere capping / Sensing of DNA Double Strand Breaks / positive regulation of telomere maintenance via telomere lengthening / meiotic telomere clustering / pre-B cell allelic exclusion / DNA-dependent protein kinase activity / histone H2AXS139 kinase activity / male meiotic nuclear division / histone mRNA catabolic process / female meiotic nuclear division / regulation of telomere maintenance via telomerase / pexophagy / cellular response to X-ray / peptidyl-serine autophosphorylation / lipoprotein catabolic process / V(D)J遺伝子再構成 / oocyte development / Impaired BRCA2 binding to PALB2 / reciprocal meiotic recombination / DNA repair complex / IgG binding / Defective homologous recombination repair (HRR) due to BRCA1 loss of function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA1 binding function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA2/RAD51/RAD51C binding function / Homologous DNA Pairing and Strand Exchange / Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA) / Resolution of D-loop Structures through Holliday Junction Intermediates / HDR through Single Strand Annealing (SSA) / Impaired BRCA2 binding to RAD51 / 1-phosphatidylinositol-3-kinase activity / response to ionizing radiation / mitotic spindle assembly checkpoint signaling / TP53 Regulates Transcription of Caspase Activators and Caspases / negative regulation of B cell proliferation / mitotic G2 DNA damage checkpoint signaling / Presynaptic phase of homologous DNA pairing and strand exchange / peroxisomal matrix / TP53 Regulates Transcription of Genes Involved in Cytochrome C Release / ヘイフリック限界 / positive regulation of cell adhesion / Regulation of HSF1-mediated heat shock response / somitogenesis / regulation of cellular response to heat / DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest / signal transduction in response to DNA damage / cellular response to retinoic acid / ovarian follicle development / negative regulation of TORC1 signaling / positive regulation of telomere maintenance via telomerase / Pexophagy / telomere maintenance / post-embryonic development / thymus development / DNA damage checkpoint signaling / regulation of signal transduction by p53 class mediator / regulation of autophagy / determination of adult lifespan / TP53 Regulates Transcription of DNA Repair Genes / Nonhomologous End-Joining (NHEJ) / Stabilization of p53 / double-strand break repair via homologous recombination / Autodegradation of the E3 ubiquitin ligase COP1 / brain development / multicellular organism growth / cellular response to gamma radiation / HDR through Homologous Recombination (HRR) / G2/M DNA damage checkpoint / Regulation of TP53 Activity through Methylation / DNA Damage/Telomere Stress Induced Senescence / spindle / 遺伝的組換え / cellular response to reactive oxygen species / double-strand break repair via nonhomologous end joining / positive regulation of neuron apoptotic process / intrinsic apoptotic signaling pathway in response to DNA damage / double-strand break repair / 細胞老化 / Regulation of TP53 Degradation / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / heart development / Processing of DNA double-strand break ends / cytoplasmic vesicle / peptidyl-serine phosphorylation / neuron apoptotic process / regulation of apoptotic process / Regulation of TP53 Activity through Phosphorylation / protein autophosphorylation / response to hypoxia / regulation of cell cycle / non-specific serine/threonine protein kinase / positive regulation of cell migration / positive regulation of apoptotic process / protein phosphorylation 類似検索 - 分子機能 | ||||||
生物種 | Streptococcus sp. group G (バクテリア) Homo sapiens (ヒト) | ||||||
手法 | 溶液NMR / simulated annealing | ||||||
データ登録者 | Abd Rahim, M.S. / Dames, S.A. | ||||||
資金援助 | ドイツ, 1件
| ||||||
引用 | ジャーナル: J.Biol.Chem. / 年: 2019 タイトル: NMR- and MD simulation-based structural characterization of the membrane-associating FATC domain of ataxia telangiectasia mutated. 著者: Abd Rahim, M.S. / Cherniavskyi, Y.K. / Tieleman, D.P. / Dames, S.A. #1: ジャーナル: Biomol NMR Assign. / 年: 2018 タイトル: 1H, 15N and 13C chemical shift assignments of the micelle immersed FAT C-terminal (FATC) domains of the human protein kinases ataxia-telangiectasia mutated (ATM) and DNA-dependent ...タイトル: 1H, 15N and 13C chemical shift assignments of the micelle immersed FAT C-terminal (FATC) domains of the human protein kinases ataxia-telangiectasia mutated (ATM) and DNA-dependent protein kinase catalytic subunit (DNA-PKcs)fused to the B1 domain of streptococcal protein G (GB1) 著者: Abd Rahim, M.S. / Sommer, L.A.M. / Wacker, A. / Schaad, M. / Dames, S.A. | ||||||
履歴 |
|
-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
---|
-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 6hka.cif.gz | 225.6 KB | 表示 | PDBx/mmCIF形式 |
---|---|---|---|---|
PDB形式 | pdb6hka.ent.gz | 178.7 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 6hka.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/hk/6hka ftp://data.pdbj.org/pub/pdb/validation_reports/hk/6hka | HTTPS FTP |
---|
-関連構造データ
類似構造データ | |
---|---|
その他のデータベース |
-リンク
-集合体
登録構造単位 |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR アンサンブル |
|
-要素
#1: 抗体 | 分子量: 11021.240 Da / 分子数: 1 / 由来タイプ: 組換発現 詳細: N-terminally GB1-tagged (1-56), linker: thrombin and enterokinase sites (57-67), FATC domain of ATM (68-100),N-terminally GB1-tagged (1-56), linker: thrombin and enterokinase sites (57-67), ...詳細: N-terminally GB1-tagged (1-56), linker: thrombin and enterokinase sites (57-67), FATC domain of ATM (68-100),N-terminally GB1-tagged (1-56), linker: thrombin and enterokinase sites (57-67), FATC domain of ATM (68-100) 由来: (組換発現) Streptococcus sp. group G (バクテリア), (組換発現) Homo sapiens (ヒト) 遺伝子: spg, ATM / プラスミド: pGEV2 / 発現宿主: Escherichia coli (大腸菌) / 株 (発現宿主): BL21 参照: UniProt: P19909, UniProt: Q13315, non-specific serine/threonine protein kinase |
---|
-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR実験 |
|
-試料調製
詳細 |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
試料 |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||
試料状態 | 詳細: 50 mM Tris, 100 mM NaCl, 150 mM DPC (usually deuterated d38-DPC) イオン強度: 100 mM / Label: condition_1 / pH: 6.5 / 圧: 1 atm / 温度: 298 K |
-NMR測定
NMRスペクトロメーター |
|
---|
-解析
NMR software |
| |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
精密化 | 手法: simulated annealing / ソフトェア番号: 4 | |||||||||||||||
代表構造 | 選択基準: lowest energy | |||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 200 / 登録したコンフォーマーの数: 20 |