A: similar to chloromuconate cycloisomerase B: similar to chloromuconate cycloisomerase C: similar to chloromuconate cycloisomerase D: similar to chloromuconate cycloisomerase E: similar to chloromuconate cycloisomerase F: similar to chloromuconate cycloisomerase G: similar to chloromuconate cycloisomerase H: similar to chloromuconate cycloisomerase ヘテロ分子
温度: 293 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 7 詳細: The protein was concentrated to 20 mg/ml and dialyzed against 5 mM HEPES, 2 mM MgCl2, 50 mM NaCl, 0.25 mM TCEP, 1 mM NaN3, pH 7.0, drop frozen as small pellets in liquid nitrogen and stored ...詳細: The protein was concentrated to 20 mg/ml and dialyzed against 5 mM HEPES, 2 mM MgCl2, 50 mM NaCl, 0.25 mM TCEP, 1 mM NaN3, pH 7.0, drop frozen as small pellets in liquid nitrogen and stored at -80 C. The frozen concentrated protein was thawed and diluted to 10 mg/mL with 5 mM HEPES, 2 mM MgCl2, 1 mM NaN3, pH 7.5. A stock solution of the peptide substrate L-Ala-L-Glu (Sigma) was neutralized to pH 7.5 with NaOH and brought to a final concentration of 1.6 M. This solution was added to the protein as 1/50th part by volume and the resultant mixture utilized for crystallization experiments. The initial crystals were grown by hanging drop by mixing 5 ?l of protein solution and 5 ?l of a solution containing 9-11% dimethyl-PEG 5000, 50 mM MOPS, 1% MPD, 1 mM NaN3, pH 7.0 and suspending the droplets over 600 ?l precipitant at 20 C, VAPOR DIFFUSION, HANGING DROP, temperature 293K
-
データ収集
回折
平均測定温度: 150 K
放射光源
由来: シンクロトロン / サイト: APS / ビームライン: 19-ID / 波長: 1 Å
検出器
タイプ: CUSTOM-MADE / 検出器: CCD / 日付: 2001年3月1日
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
解像度: 2.1→30 Å / Cor.coef. Fo:Fc: 0.954 / Cor.coef. Fo:Fc free: 0.917 / SU B: 7.705 / SU ML: 0.184 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.269 / ESU R Free: 0.231 / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.285
8685
5 %
RANDOM
Rwork
0.219
-
-
-
all
0.222
173500
-
-
obs
0.222
164780
96.51 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: BABINET MODEL WITH MASK