+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1mpv | ||||||
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タイトル | Structure of bhpBR3, the BAFF-binding loop of BR3 embedded in a beta-hairpin peptide | ||||||
要素 | BLyS Receptor 3 | ||||||
キーワード | PROTEIN BINDING (タンパク質) / beta-hairpin (Βヘアピン) | ||||||
機能・相同性 | 機能・相同性情報 B cell costimulation / TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway / positive regulation of B cell proliferation / positive regulation of T cell proliferation / tumor necrosis factor-mediated signaling pathway / T cell costimulation / TNFR2 non-canonical NF-kB pathway / signaling receptor activity / 獲得免疫系 / external side of plasma membrane / 細胞膜 類似検索 - 分子機能 | ||||||
手法 | 溶液NMR / hybrid distance geometry, simulated annealing, restrained molecular dynamics. | ||||||
データ登録者 | Kayagaki, N. / Yan, M. / Seshasayee, D. / Wang, H. / Lee, W. / French, D.M. / Grewal, I.S. / Cochran, A.G. / Gordon, N.C. / Yin, J. ...Kayagaki, N. / Yan, M. / Seshasayee, D. / Wang, H. / Lee, W. / French, D.M. / Grewal, I.S. / Cochran, A.G. / Gordon, N.C. / Yin, J. / Starovasnik, M.A. / Dixit, V.M. | ||||||
引用 | ジャーナル: Immunity / 年: 2002 タイトル: BAFF/BLyS receptor 3 binds the B cell survival factor BAFF ligand through a discrete surface loop and promotes processing of NF-kappaB2. 著者: Kayagaki, N. / Yan, M. / Seshasayee, D. / Wang, H. / Lee, W. / French, D.M. / Grewal, I.S. / Cochran, A.G. / Gordon, N.C. / Yin, J. / Starovasnik, M.A. / Dixit, V.M. | ||||||
履歴 |
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Remark 999 | SEQUENCE THIS IS PART OF AN OPTIMIZED BETA-HAIRPIN SCAFFOLD, (SEE RUSSELL, BLANDL, SKELTON, & ...SEQUENCE THIS IS PART OF AN OPTIMIZED BETA-HAIRPIN SCAFFOLD, (SEE RUSSELL, BLANDL, SKELTON, & COCHRAN, "STABILITY OF CYCLIC BETA-HAIRPINS: ASYMMETRIC CONTRIBUTIONS FROM SIDE CHAINS OF A HYDROGEN-BONDED CROSS-STRAND RESIDUE PAIR", J. AMER. CHEM. SOC., IN PRESS.) |
-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1mpv.cif.gz | 78.5 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1mpv.ent.gz | 58.4 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1mpv.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/mp/1mpv ftp://data.pdbj.org/pub/pdb/validation_reports/mp/1mpv | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質・ペプチド | 分子量: 1593.918 Da / 分子数: 1 / 断片: BR3 loop (residues 26-31) / 由来タイプ: 合成 詳細: The Peptide was synthesized chemically with acetylated N-terminus and amidated C-terminus 参照: UniProt: Q96RJ3 |
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-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||
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NMR実験 |
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-試料調製
詳細 |
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試料状態 | イオン強度: no added salt / pH: 4.5 / 圧: ambient / 温度: 293 K | |||||||||
結晶化 | *PLUS 手法: その他 / 詳細: NMR |
-NMR測定
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M |
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放射波長 | 相対比: 1 |
NMRスペクトロメーター | タイプ: Bruker DRX / 製造業者: Bruker / モデル: DRX / 磁場強度: 600 MHz |
-解析
NMR software |
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精密化 | 手法: hybrid distance geometry, simulated annealing, restrained molecular dynamics. ソフトェア番号: 1 詳細: Complete 1H resonance assignments were obtained using standard 2D homonuclear NMR methods. Distance restraints were derived from analysis of a 2D NOESY spectrum (250ms mixing time); HN-Ha ...詳細: Complete 1H resonance assignments were obtained using standard 2D homonuclear NMR methods. Distance restraints were derived from analysis of a 2D NOESY spectrum (250ms mixing time); HN-Ha coupling constants were obtained from analysis of a DQF-COSY spectrum acquired in water; and HaHb values were obtained from analysis of a COSY-35 spectrum acquired in D2O. Structures were calculated from a total of 119 NOE-derived (including 46 long-range) distance restraints and 16 dihedral angle restraints. Structures satisfy the experimental data very well with no distance or dihedral angle violations greater than 0.1 angstrom or 1 degree, respectively. | ||||||||||||||||||||
代表構造 | 選択基準: closest to the average | ||||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the least restraint violations 計算したコンフォーマーの数: 80 / 登録したコンフォーマーの数: 20 |