+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-4303 | |||||||||
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タイトル | Cryo-EM subtomogram average of IFT complex B and inhibited dynein-1b in anterograde IFT trains (Chlamydomonas reinhardtii) | |||||||||
マップデータ | Cryo-EM stubtomogram average of IFT complex B and inhibited dynein-1b in anterograde IFT trains (Chlamydomonas reinhardtii) | |||||||||
試料 |
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機能・相同性 | 機能・相同性情報 intraciliary transport involved in cilium assembly / nitric-oxide synthase inhibitor activity / deoxyribonuclease inhibitor activity / negative regulation of DNA strand resection involved in replication fork processing / organelle organization / visual behavior / intraciliary retrograde transport / methylated-DNA-[protein]-cysteine S-methyltransferase / methylated-DNA-[protein]-cysteine S-methyltransferase activity / intraciliary transport ...intraciliary transport involved in cilium assembly / nitric-oxide synthase inhibitor activity / deoxyribonuclease inhibitor activity / negative regulation of DNA strand resection involved in replication fork processing / organelle organization / visual behavior / intraciliary retrograde transport / methylated-DNA-[protein]-cysteine S-methyltransferase / methylated-DNA-[protein]-cysteine S-methyltransferase activity / intraciliary transport / dynein light chain binding / ciliary transition zone / regulation of cilium assembly / motile cilium assembly / dynein heavy chain binding / Activation of BIM and translocation to mitochondria / embryonic skeletal system morphogenesis / ciliary tip / Intraflagellar transport / negative regulation of nitric oxide biosynthetic process / dynein complex / cell projection organization / negative regulation of phosphorylation / COPI-independent Golgi-to-ER retrograde traffic / minus-end-directed microtubule motor activity / dynein light intermediate chain binding / cytoplasmic dynein complex / ciliary plasm / motile cilium / enzyme inhibitor activity / determination of left/right symmetry / microtubule motor activity / ciliary base / dynein intermediate chain binding / Macroautophagy / pericentriolar material / microtubule-based movement / axoneme / positive regulation of insulin secretion involved in cellular response to glucose stimulus / tertiary granule membrane / ficolin-1-rich granule membrane / spermatid development / cilium assembly / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / COPI-mediated anterograde transport / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Loss of Nlp from mitotic centrosomes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of mitotic centrosome proteins and complexes / axon cytoplasm / Recruitment of NuMA to mitotic centrosomes / Anchoring of the basal body to the plasma membrane / Resolution of Sister Chromatid Cohesion / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / substantia nigra development / MHC class II antigen presentation / centriole / AURKA Activation by TPX2 / ciliary basal body / filopodium / RHO GTPases Activate Formins / HCMV Early Events / cilium / mitotic spindle / kinetochore / Aggrephagy / Separation of Sister Chromatids / Regulation of PLK1 Activity at G2/M Transition / apical part of cell / site of double-strand break / scaffold protein binding / methylation / microtubule / cytoskeleton / DNA repair / centrosome / DNA damage response / Neutrophil degranulation / protein-containing complex binding / apoptotic process / enzyme binding / Golgi apparatus / ATP hydrolysis activity / mitochondrion / DNA binding / extracellular space / ATP binding / identical protein binding / membrane / nucleus / metal ion binding / plasma membrane / cytoplasm / cytosol 類似検索 - 分子機能 | |||||||||
生物種 | Chlamydomonas reinhardtii (クラミドモナス) | |||||||||
手法 | サブトモグラム平均法 / クライオ電子顕微鏡法 / 解像度: 37.0 Å | |||||||||
データ登録者 | Pigino G / Jordan MA | |||||||||
引用 | ジャーナル: Nat Cell Biol / 年: 2018 タイトル: The cryo-EM structure of intraflagellar transport trains reveals how dynein is inactivated to ensure unidirectional anterograde movement in cilia. 著者: Mareike A Jordan / Dennis R Diener / Ludek Stepanek / Gaia Pigino / 要旨: Movement of cargos along microtubules plays key roles in diverse cellular processes, from signalling to mitosis. In cilia, rapid movement of ciliary components along the microtubules to and from the ...Movement of cargos along microtubules plays key roles in diverse cellular processes, from signalling to mitosis. In cilia, rapid movement of ciliary components along the microtubules to and from the assembly site is essential for the assembly and disassembly of the structure itself. This bidirectional transport, known as intraflagellar transport (IFT), is driven by the anterograde motor kinesin-2 and the retrograde motor dynein-1b (dynein-2 in mammals). However, to drive retrograde transport, dynein-1b must first be delivered to the ciliary tip by anterograde IFT. Although, the presence of opposing motors in bidirectional transport processes often leads to periodic stalling and slowing of cargos, IFT is highly processive. Using cryo-electron tomography, we show that a tug-of-war between kinesin-2 and dynein-1b is prevented by loading dynein-1b onto anterograde IFT trains in an autoinhibited form and by positioning it away from the microtubule track to prevent binding. Once at the ciliary tip, dynein-1b must transition into an active form and engage microtubules to power retrograde trains. These findings provide a striking example of how coordinated structural changes mediate the behaviour of complex cellular machinery. | |||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_4303.map.gz | 168.2 KB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-4303-v30.xml emd-4303.xml | 8.9 KB 8.9 KB | 表示 表示 | EMDBヘッダ |
画像 | emd_4303_1.png emd_4303_2.png | 284.4 KB 188 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-4303 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-4303 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_4303_validation.pdf.gz | 203.2 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_4303_full_validation.pdf.gz | 202.3 KB | 表示 | |
XML形式データ | emd_4303_validation.xml.gz | 4.6 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-4303 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-4303 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_4303.map.gz / 形式: CCP4 / 大きさ: 306.6 KB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | Cryo-EM stubtomogram average of IFT complex B and inhibited dynein-1b in anterograde IFT trains (Chlamydomonas reinhardtii) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 14.13 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-試料の構成要素
-全体 : Intraflagellar transport complex B and inhibited dynein-1b
全体 | 名称: Intraflagellar transport complex B and inhibited dynein-1b |
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要素 |
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-超分子 #1: Intraflagellar transport complex B and inhibited dynein-1b
超分子 | 名称: Intraflagellar transport complex B and inhibited dynein-1b タイプ: complex / ID: 1 / 親要素: 0 |
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由来(天然) | 生物種: Chlamydomonas reinhardtii (クラミドモナス) 株: CC-124 |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | サブトモグラム平均法 |
試料の集合状態 | 2D array |
-試料調製
緩衝液 | pH: 7 |
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グリッド | モデル: Quantifoil R3.5/1 / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: HOLEY / 前処理 - タイプ: GLOW DISCHARGE |
凍結 | 凍結剤: ETHANE / 装置: LEICA EM GP |
-電子顕微鏡法
顕微鏡 | FEI TITAN |
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特殊光学系 | エネルギーフィルター - 名称: GIF Quantum LS |
撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 検出モード: SUPER-RESOLUTION / 平均電子線量: 2.1 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD |
-画像解析
最終 再構成 | 想定した対称性 - 点群: C1 (非対称) / 解像度のタイプ: BY AUTHOR / 解像度: 37.0 Å / 解像度の算出法: FSC 0.5 CUT-OFF / ソフトウェア - 名称: PEET (ver. 1.11.0) / 使用したサブトモグラム数: 750 |
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抽出 | トモグラム数: 15 / 使用した粒子像数: 750 / ソフトウェア - 名称: IMOD (ver. 4.9.2) |
CTF補正 | ソフトウェア - 名称: CTFPHASEFLIP |
最終 角度割当 | タイプ: NOT APPLICABLE |