Ion transport by P-type ATPases / H+/K+-exchanging ATPase / Na+/K+-ATPアーゼ / P-type potassium:proton transporter activity / positive regulation of sodium ion export across plasma membrane / positive regulation of potassium ion import across plasma membrane / metal ion transport / P-type sodium:potassium-exchanging transporter activity / membrane repolarization / sodium:potassium-exchanging ATPase complex ...Ion transport by P-type ATPases / H+/K+-exchanging ATPase / Na+/K+-ATPアーゼ / P-type potassium:proton transporter activity / positive regulation of sodium ion export across plasma membrane / positive regulation of potassium ion import across plasma membrane / metal ion transport / P-type sodium:potassium-exchanging transporter activity / membrane repolarization / sodium:potassium-exchanging ATPase complex / sodium ion export across plasma membrane / regulation of calcium ion transmembrane transport / intracellular potassium ion homeostasis / regulation of pH / intracellular sodium ion homeostasis / potassium ion homeostasis / regulation of cardiac muscle contraction by calcium ion signaling / response to metal ion / relaxation of cardiac muscle / Ion homeostasis / sodium ion transport / potassium ion import across plasma membrane / ATPase activator activity / monoatomic cation transmembrane transport / blastocyst development / organelle membrane / sodium ion transmembrane transport / 介在板 / lateral plasma membrane / sperm flagellum / ATP metabolic process / cardiac muscle contraction / 横行小管 / proton transmembrane transport / カベオラ / protein localization to plasma membrane / 筋鞘 / potassium ion transport / response to organic cyclic compound / intracellular calcium ion homeostasis / protein-macromolecule adaptor activity / ATPase binding / 遺伝子発現の調節 / basolateral plasma membrane / response to hypoxia / protein stabilization / 細胞接着 / apical plasma membrane / protein heterodimerization activity / 自然免疫系 / protein kinase binding / ATP hydrolysis activity / ATP binding / 生体膜 / metal ion binding / 細胞膜 類似検索 - 分子機能
ジャーナル: Nat Commun / 年: 2022 タイトル: Structure and function of H/K pump mutants reveal Na/K pump mechanisms. 著者: Victoria C Young / Hanayo Nakanishi / Dylan J Meyer / Tomohiro Nishizawa / Atsunori Oshima / Pablo Artigas / Kazuhiro Abe / 要旨: Ion-transport mechanisms evolve by changing ion-selectivity, such as switching from Na to H selectivity in secondary-active transporters or P-type-ATPases. Here we study primary-active transport via ...Ion-transport mechanisms evolve by changing ion-selectivity, such as switching from Na to H selectivity in secondary-active transporters or P-type-ATPases. Here we study primary-active transport via P-type ATPases using functional and structural analyses to demonstrate that four simultaneous residue substitutions transform the non-gastric H/K pump, a strict H-dependent electroneutral P-type ATPase, into a bona fide Na-dependent electrogenic Na/K pump. Conversion of a H-dependent primary-active transporter into a Na-dependent one provides a prototype for similar studies of ion-transport proteins. Moreover, we solve the structures of the wild-type non-gastric H/K pump, a suitable drug target to treat cystic fibrosis, and of its Na/K pump-mimicking mutant in two major conformations, providing insight on how Na binding drives a concerted mechanism leading to Na/K pump phosphorylation.