+
データを開く
-
基本情報
登録情報 | データベース: SASBDB / ID: SASDBV4 |
---|---|
![]() | Vaccinia virus MVA F1L antiapoptotic Bcl-2 viral protein
|
機能・相同性 | ![]() host cell mitochondrial outer membrane / symbiont-mediated perturbation of host apoptosis / symbiont-mediated suppression of host apoptosis / regulation of apoptotic process / membrane 類似検索 - 分子機能 |
生物種 | ![]() ![]() |
![]() | ![]() タイトル: The N Terminus of the Vaccinia Virus Protein F1L Is an Intrinsically Unstructured Region That Is Not Involved in Apoptosis Regulation. 著者: Sofia Caria / Bevan Marshall / Robyn-Lee Burton / Stephanie Campbell / Delara Pantaki-Eimany / Christine J Hawkins / Michele Barry / Marc Kvansakul / ![]() ![]() 要旨: Subversion of host cell apoptotic responses is a prominent feature of viral immune evasion strategies to prevent premature clearance of infected cells. Numerous poxviruses encode structural and ...Subversion of host cell apoptotic responses is a prominent feature of viral immune evasion strategies to prevent premature clearance of infected cells. Numerous poxviruses encode structural and functional homologs of the Bcl-2 family of proteins, and vaccinia virus harbors antiapoptotic F1L that potently inhibits the mitochondrial apoptotic checkpoint. Recently F1L has been assigned a caspase-9 inhibitory function attributed to an N-terminal α helical region of F1L spanning residues 1-15 (1) preceding the domain-swapped Bcl-2-like domains. Using a reconstituted caspase inhibition assay in yeast we found that unlike AcP35, a well characterized caspase-9 inhibitor from the insect virus Autographa californica multiple nucleopolyhedrovirus, F1L does not prevent caspase-9-mediated yeast cell death. Furthermore, we found that deletion of the F1L N-terminal region does not impede F1L antiapoptotic activity in the context of a viral infection. Solution analysis of the F1L N-terminal regions using small angle x-ray scattering indicates that the region of F1L spanning residues 1-50 located N-terminally from the Bcl-2 fold is an intrinsically unstructured region. We conclude that the N terminus of F1L is not involved in apoptosis inhibition and may act as a regulatory element in other signaling pathways in a manner reminiscent of other unstructured regulatory elements commonly found in mammalian prosurvival Bcl-2 members including Bcl-xL and Mcl-1. |
![]() |
|
-
構造の表示
構造ビューア | 分子: ![]() ![]() |
---|
-
ダウンロードとリンク
-モデル
モデル #535 | ![]() タイプ: mix / ソフトウェア: BUNCH / ダミー原子の半径: 1.90 A / カイ2乗値: 7.29 ![]() |
---|---|
モデル #536 | ![]() タイプ: mix / ソフトウェア: CORAL / ダミー原子の半径: 1.90 A / カイ2乗値: 3.8416 ![]() |
-
試料
![]() | 名称: Vaccinia virus MVA F1L antiapoptotic Bcl-2 viral protein 試料濃度: 0.12-3.60 |
---|---|
バッファ | 名称: 25 mM HEPES, 150 mM NaCl, 5 mM DTT / pH: 7.5 |
要素 #341 | 名称: MVA F1L (Protein F1) / タイプ: protein / 記述: MVA F1L antiapoptotic Bcl-2 viral protein / 分子量: 25.291 / 分子数: 2 / 由来: Vaccinia virus / 参照: UniProt: O57173 配列: MGSSHHHHHH SQDPMLSMFM CNNIVDYVDG IVQDIEDEAS NNVDHDYVYP LPENMVYRFD KSTNILDYLS TERDHVMMAV RYYMSKQRLD DLYRQLPTKT RSYIDIINIY CDKVSNDYNR DMNIMYDMAS TKSFTVYDIN NEVNTILMDN KGLGVRLATI SFITELGRRC ...配列: MGSSHHHHHH SQDPMLSMFM CNNIVDYVDG IVQDIEDEAS NNVDHDYVYP LPENMVYRFD KSTNILDYLS TERDHVMMAV RYYMSKQRLD DLYRQLPTKT RSYIDIINIY CDKVSNDYNR DMNIMYDMAS TKSFTVYDIN NEVNTILMDN KGLGVRLATI SFITELGRRC MNPVKTIKMF TLLSHTICDD CFVDYITDIS PPDNTIPNTS TREYLK |
-実験情報
ビーム | 設備名称: Australian Synchrotron SAXS/WAXS / 地域: Melbourne / 国: Australia ![]() | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
検出器 | 名称: Pilatus 1M | ||||||||||||||||||
スキャン |
| ||||||||||||||||||
距離分布関数 P(R) |
| ||||||||||||||||||
結果 |
|