SASDBF2: Solution structure of 40bp long dsDNA-Sa Oligonucleotide

Basic information

• Entry: Database: SASBDB / ID: SASDBF2

Sample

Solution structure of 40bp long dsDNA-Sa Oligonucleotide

• 40bp long dsDNA-Sa Oligonucleotide (DNA), dsDNA-Sa Oligo

Contact author

• Ankan Banerjee (Philipps-Universität Marburg, Marburg, Germany)

Models

• Model #397: ; Type: dummy / Radius of dummy atoms: 2.50 A / Chi-square value: 1.461; Search similar-shape structures of this assembly by Omokage search (details)

Sample

• Sample: Name: Solution structure of 40bp long dsDNA-Sa Oligonucleotide; Specimen concentration: 1 mg/ml
• Buffer: Name: 0.5 x Tris/Borate/EDTA (TBE) / Concentration: 0.50 v/v

Entity #262

Name: dsDNA-Sa Oligo / Type: DNA / Description: 40bp long dsDNA-Sa Oligonucleotide / Formula weight: 24.7 / Num. of mol.: 1
Sequence:

GTAGTAAGAT TTAAAAAAAC ATACTAAGAT ATAAATTAAC

Experimental information

• Beam: Instrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.93 Å / Dist. spec. to detc.: 2.43 mm
• Detector: Name: Pilatus 1M

Scan

Title: 40bp long dsDNA-Sa Oligonucleotide / Measurement date: Aug 28, 2015 / Storage temperature: 4 °C / Cell temperature: 4 °C / Exposure time: 10 sec. / Number of frames: 20 / Unit: 1/nm /

	Min	Max
Q	0.0327	4.9336

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 783 /

	Min	Max
Q	0.112644	3.79068
P(R) point	1	783
R	0	12

Result

Type of curve: single_conc /

	Experimental	Porod
MW	82 kDa	18.3 kDa

	P(R)	Guinier
Forward scattering, I0	82.41	82
Radius of gyration, Rg	3.55 nm	3.56 nm

	Min	Max
D	-	12
Guinier point	16	57