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- SASDFM6: Adenine specific DNA methyltransferase (Mod HP_0593) at pH 5.5 -

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Open data


ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDFM6
SampleAdenine specific DNA methyltransferase (Mod HP_0593) at pH 5.5
  • Adenine specific DNA methyltransferase (Mod) (protein), Helicobacter pylori (strain ATCC 700392 / 26695)
Function / homology
Function and homology information


N-methyltransferase activity / site-specific DNA-methyltransferase (adenine-specific) / site-specific DNA-methyltransferase (adenine-specific) activity / methyltransferase activity / methylation / DNA binding / cytoplasm
Similarity search - Function
N4/N6-methyltransferase, Type III restriction-modification enzyme EcoPI Mod subunit-like / DNA methylase N-4/N-6 / DNA methylase / N-6 Adenine-specific DNA methylases signature. / DNA methylase, N-6 adenine-specific, conserved site / S-adenosyl-L-methionine-dependent methyltransferase superfamily
Similarity search - Domain/homology
site-specific DNA-methyltransferase (adenine-specific)
Similarity search - Component
Biological speciesHelicobacter pylori (strain ATCC 700392 / 26695) (bacteria)
Contact author
  • Naveen Narayanan (RCB, Regional Centre for Biotechnology, Haryana, India)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #3045
Type: atomic / Chi-square value: 1.796
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Adenine specific DNA methyltransferase (Mod HP_0593) at pH 5.5
Specimen concentration: 2 mg/ml
BufferName: 25 mM citrate, 250 mM NaCl / pH: 5.5
Entity #1668Type: protein / Description: Adenine specific DNA methyltransferase (Mod) / Formula weight: 68.367 / Num. of mol.: 4 / Source: Helicobacter pylori (strain ATCC 700392 / 26695) / References: UniProt: O25315
Sequence: MLLKNFPQTI KDGQVSLSAI KMLLGFNESM NDISGYELTW TGKGFANALY SEPCQKQLKL QESFTPQTSA SKHPNNAIII GDNLDALKLL KSAYSEKIKM IYIDPPYNTG NDEFIYPDNF RQDYQKILRE VGLMEIDENG KEIESESLKF FKNTQGSGTH SGWLSFMLPR ...Sequence:
MLLKNFPQTI KDGQVSLSAI KMLLGFNESM NDISGYELTW TGKGFANALY SEPCQKQLKL QESFTPQTSA SKHPNNAIII GDNLDALKLL KSAYSEKIKM IYIDPPYNTG NDEFIYPDNF RQDYQKILRE VGLMEIDENG KEIESESLKF FKNTQGSGTH SGWLSFMLPR LKLARDLLKE DGVIFISIDD NECANLKILC DEIFGEDNFV GDFIRKTKST TNDAKIGLNY QHEFLLCYAK DKNYTNLLGG EKNLENYKNP DNDPNGAWIN DNPSAKSGNM KTGYFGVTNP YTNKVDYPPV GMFWRFSQNT IQKHIDEGRI CFKKEHKDNE RGFIYKRYLK DLKTTQKTFD SLIFSDNCYM NQAATKELLN LGMGEYFTYP KGVEFMKKII LHSTTPNEGD IILDFFAGSG TTVHAVMELN AEDKGNREFI LVQIDEEIKE DESAYDFCKK ELKSAKPVIS DITIERVKRA AQKISQLSKD SGLDLGFKVY TLQDKVQIIN DKEEITLFNR SDLTPFDKAL NLALQCGKTL NQALEIIIKD KLYKCEDAYF CIVCDEEAQE YLAKSKNEMI FLDGYEEIDL EAFLNLNASF KERLSVVY

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Experimental information

BeamInstrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotron
DetectorName: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm
Scan
Title: Adenine specific DNA methyltransferase (Mod), HP_0593, pH 5.5
Measurement date: Dec 1, 2018 / Unit: 1/nm /
MinMax
Q0.0375 4.9462
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 306 /
MinMax
Q0.164703 1.60152
P(R) point1 306
R0 19.1
Result
Type of curve: single_conc
Comments: The sample is severely aggregated. Wavelength UNKNOWN; Experimental temperature, UNKNOWN; Exposure time, UNKNOWN; Sample to detector distance, UNKNOWN.
ExperimentalPorod
MW250 kDa209 kDa
Volume-316 nm3

P(R)GuinierGuinier error
Forward scattering, I0122.9 126.76 0.8
Radius of gyration, Rg4.91 nm4.98 nm0.42

MinMax
D-19.1
Guinier point18 47

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