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データを開く
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基本情報
登録情報 | データベース: SASBDB / ID: SASDFS3 |
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![]() | Murine transcription intermediary factor 1-beta, TRIM28 RBCC assembly-null mutation R184D
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機能・相同性 | ![]() Generic Transcription Pathway / convergent extension involved in axis elongation / : / Krueppel-associated box domain binding / embryonic placenta morphogenesis / positive regulation of DNA methylation-dependent heterochromatin formation / suppression of viral release by host / epigenetic programming of gene expression / genomic imprinting / negative regulation of single stranded viral RNA replication via double stranded DNA intermediate ...Generic Transcription Pathway / convergent extension involved in axis elongation / : / Krueppel-associated box domain binding / embryonic placenta morphogenesis / positive regulation of DNA methylation-dependent heterochromatin formation / suppression of viral release by host / epigenetic programming of gene expression / genomic imprinting / negative regulation of single stranded viral RNA replication via double stranded DNA intermediate / chromo shadow domain binding / protein sumoylation / epithelial to mesenchymal transition / heterochromatin / positive regulation of DNA repair / embryo implantation / promoter-specific chromatin binding / euchromatin / RING-type E3 ubiquitin transferase / positive regulation of protein import into nucleus / RNA polymerase II transcription regulator complex / transcription corepressor activity / ubiquitin protein ligase activity / chromatin organization / in utero embryonic development / transcription coactivator activity / protein kinase activity / DNA repair / innate immune response / negative regulation of DNA-templated transcription / ubiquitin protein ligase binding / chromatin binding / chromatin / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / DNA binding / zinc ion binding / nucleoplasm / nucleus 類似検索 - 分子機能 |
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![]() | ![]() タイトル: A Dissection of Oligomerization by the TRIM28 Tripartite Motif and the Interaction with Members of the Krab-ZFP Family. 著者: Yunyuan Sun / Jeremy R Keown / Moyra M Black / Charlène Raclot / Nicholas Demarais / Didier Trono / Priscilla Turelli / David C Goldstone / ![]() ![]() 要旨: TRIM28 (also known as KAP1 or TIF1β) is the universal co-repressor of the Krüppel-associated box-containing zinc finger proteins (Krab-ZFPs), the largest family of transcription factors in mammals. ...TRIM28 (also known as KAP1 or TIF1β) is the universal co-repressor of the Krüppel-associated box-containing zinc finger proteins (Krab-ZFPs), the largest family of transcription factors in mammals. During early embryogenesis, TRIM28 mediates the transcriptional silencing of many endogenous retroviral elements and genomic imprinted sites. Silencing is initiated by the recruitment of TRIM28 to a target locus by members of the Krab-ZFP. Subsequently, TRIM28 functions as a scaffold protein to recruit chromatin modifying effectors featuring SETDB1, HP1 and the NuRD complex. Although many protein partners involved in silencing have been identified, the molecular basis of the protein interactions that mediate silencing remains largely unclear. In the present study, we identified the first Bbox domain (T28_B1 135-203) as a molecular interface responsible for the formation of higher-order oligomers of TRIM28. The structure of this domain reveals a new interface on the surface of the Bbox domain. Mutants disrupting the interface disrupt the formation of oligomers but have no observed effect on transcriptional silencing defining a single TRIM28 dimer as the functional unit for silencing. Using assembly-deficient mutants, we employed small-angle X-ray scattering and biophysical techniques to characterize binding to member of the Krab-ZFP family. This allows us to narrow and define the binding interface to the center of the coiled-coil region (residues 294-321) of TRIM28 and define mutants that abolish binding to the Krab-ZFP proteins. |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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-モデル
モデル #2839 | ![]() タイプ: dummy / ソフトウェア: (2.3i) / ダミー原子の半径: 1.90 A / 対称性: p2 / カイ2乗値: 0.33 / P-value: 0.012949 ![]() |
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試料
![]() | 名称: Murine transcription intermediary factor 1-beta, TRIM28 RBCC assembly-null mutation R184D 試料濃度: 0.15-0.20 |
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バッファ | 名称: 10 mM Tris 300 mM NaCl 0.1 mM TCEP / pH: 8 |
要素 #1498 | 名称: TRIM28 / タイプ: protein / 記述: Transcription intermediary factor 1-beta / 分子量: 40.928 / 分子数: 2 / 由来: Mus musculus / 参照: UniProt: Q62318 配列: GPGEAQELLE HCGVCRERLR PERDPRLLPC LHSACSACLG PATPAAANNS GDGGSAGDGA MVDCPVCKQQ CYSKDIVENY FMRDSGSKAS SDSQDANQCC TSCEDNAPAT SYCVECSEPL CETCVEAHQR VKYTKDHTVR STGPAKTRDG ERTVYCNVHK HEPLVLFCES ...配列: GPGEAQELLE HCGVCRERLR PERDPRLLPC LHSACSACLG PATPAAANNS GDGGSAGDGA MVDCPVCKQQ CYSKDIVENY FMRDSGSKAS SDSQDANQCC TSCEDNAPAT SYCVECSEPL CETCVEAHQR VKYTKDHTVR STGPAKTRDG ERTVYCNVHK HEPLVLFCES CDTLTCRDCQ LNAHKDHQYQ FLEDAVRNQR KLLASLVKRL GDKHATLQKN TKEVRSSIRQ VSDVQKRVQV DVKMAILQIM KELNKRGRVL VNDAQKVTEG QQERLERQHW TMTKIQKHQE HILRFASWAL ESDNNTALLL SKKLIYFQLH RALKMIVDPV EPHGEMKFQW DLNAWTKSAE AFGKIVAERP GTNS |
-実験情報
ビーム | 設備名称: Australian Synchrotron SAXS/WAXS / 地域: Melbourne / 国: Australia ![]() | |||||||||||||||||||||||||||||||||
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検出器 | 名称: Pilatus 1M / タイプ: Dectris / Pixsize x: 172 mm | |||||||||||||||||||||||||||||||||
スキャン | 測定日: 2017年8月10日 / セル温度: 16 °C / 照射時間: 1 sec. / フレーム数: 12 / 単位: 1/A /
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