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- SASDAK7: Metal-free hETHE1, 2 mg/ml (Persulfide dioxygenase ETHE1, mitocho... -

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Basic information

Entry
Database: SASBDB / ID: SASDAK7
SampleMetal-free hETHE1, 2 mg/ml
  • Persulfide dioxygenase ETHE1, mitochondrial (protein), hETHE1, Homo sapiens
Function / homology
Function and homology information


persulfide dioxygenase / hydrogen sulfide metabolic process / Sulfide oxidation to sulfate / sulfur dioxygenase activity / glutathione metabolic process / mitochondrial matrix / iron ion binding / mitochondrion / nucleoplasm / identical protein binding / cytoplasm
Similarity search - Function
Persulfide dioxygenase-like, MBL-fold metallo-hydrolase domain / : / Metallo-beta-lactamase superfamily / Metallo-beta-lactamase superfamily / Metallo-beta-lactamase / Ribonuclease Z/Hydroxyacylglutathione hydrolase-like
Similarity search - Domain/homology
Persulfide dioxygenase ETHE1, mitochondrial
Similarity search - Component
Biological speciesHomo sapiens (human)
Contact author
  • Al Kikhney
  • Marco Salomone-Stagni

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #283
Type: dummy / Software: DAMMIN / Radius of dummy atoms: 10.90 A / Chi-square value: 1.361
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Metal-free hETHE1, 2 mg/ml / Specimen concentration: 2 mg/ml
BufferName: Tris / Concentration: 50.00 mM / pH: 8
Comment: TCEP does not reduce metals in solution, but prevents aggregation and precipitation of the protein
Composition: 150 mM NaCl 2 mM TCEP
Entity #157Name: hETHE1 / Type: protein / Description: Persulfide dioxygenase ETHE1, mitochondrial / Formula weight: 27.873 / Num. of mol.: 2 / Source: Homo sapiens / References: UniProt: O95571

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Experimental information

BeamInstrument name: DORIS III X33 / City: Hamburg / 国: Germany / Shape: 0.6 / Type of source: X-ray synchrotron / Wavelength: 0.15 Å / Dist. spec. to detc.: 2.7 mm
DetectorName: Pilatus 1M-W / Pixsize x: 0.172 mm
Scan
Title: Metal-free hETHE1, 2 mg/ml / Measurement date: Nov 23, 2009 / Cell temperature: 5 °C / Exposure time: 30 sec. / Number of frames: 4 / Unit: 1/nm /
MinMax
Q0.0775 6.237
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 219 /
MinMax
Q0.106798 0.688341
P(R) point1 219
R0 42.5
Result
Type of curve: single_conc
ExperimentalPorodEstimatedEstimated method
MW358 kDa405 kDa--
Volume-647 nm3716 DAMMIN

P(R)GuinierGuinier error
Forward scattering, I0244.4 220.1 10.04
Radius of gyration, Rg10.64 nm8.83 nm1.16

MinMax
D-42.5
Guinier point12 25

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