SASDF53: Bruton's Tyrosine Kinase - kinase domain (Bruton's tyrosine kinas...

Basic information

• Entry: Database: SASBDB / ID: SASDF53

Sample

Bruton's Tyrosine Kinase - kinase domain

• Bruton's tyrosine kinase, kinase domain (protein), Btk-KD, Homo sapiens

Function / homology

Function:

regulation of B cell cytokine production / proteoglycan catabolic process / monocyte proliferation / positive regulation of interleukin-17A production / regulation of B cell apoptotic process / eosinophil homeostasis / positive regulation of type III hypersensitivity / B cell affinity maturation / positive regulation of synoviocyte proliferation / histamine secretion by mast cell / neutrophil homeostasis / cellular response to molecule of fungal origin / positive regulation of type I hypersensitivity / MyD88 deficiency (TLR2/4) / cellular response to interleukin-7 / MyD88-dependent toll-like receptor signaling pathway / IRAK4 deficiency (TLR2/4) / MyD88:MAL(TIRAP) cascade initiated on plasma membrane / positive regulation of immunoglobulin production / positive regulation of B cell differentiation / positive regulation of NLRP3 inflammasome complex assembly / phospholipase activator activity / negative regulation of interleukin-10 production / negative regulation of B cell proliferation / mesoderm development / Fc-epsilon receptor signaling pathway / B cell activation / phosphatidylinositol-3,4,5-trisphosphate binding / phospholipase binding / RHO GTPases Activate WASPs and WAVEs / positive regulation of phagocytosis / positive regulation of B cell proliferation / cell maturation / FCERI mediated Ca+2 mobilization / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / apoptotic signaling pathway / non-specific protein-tyrosine kinase / FCGR3A-mediated phagocytosis / B cell receptor signaling pathway / non-membrane spanning protein tyrosine kinase activity / calcium-mediated signaling / Regulation of actin dynamics for phagocytic cup formation / peptidyl-tyrosine phosphorylation / G beta:gamma signalling through BTK / positive regulation of interleukin-6 production / cellular response to reactive oxygen species / positive regulation of tumor necrosis factor production / G alpha (12/13) signalling events / DAP12 signaling / positive regulation of NF-kappaB transcription factor activity / T cell receptor signaling pathway / ER-Phagosome pathway / cytoplasmic vesicle / protein tyrosine kinase activity / G alpha (q) signalling events / response to lipopolysaccharide / adaptive immune response / Potential therapeutics for SARS / intracellular signal transduction / protein phosphorylation / membrane raft / innate immune response / perinuclear region of cytoplasm / ATP binding / identical protein binding / nucleus / metal ion binding / plasma membrane / cytosol / cytoplasm

Domain/homology:

Tyrosine-protein kinase BTK, SH3 domain / Zinc finger, Btk motif / BTK motif / Zinc finger Btk-type profile. / Bruton's tyrosine kinase Cys-rich motif / : / PH domain / PH domain profile. / Pleckstrin homology domain. / Pleckstrin homology domain / SH3 domain / SH2 domain / Src homology 2 (SH2) domain profile. / Src homology 2 domains / SH2 domain / Src homology 3 domains / SH2 domain superfamily / SH3-like domain superfamily / Src homology 3 (SH3) domain profile. / SH3 domain / Tyrosine-protein kinase, catalytic domain / Tyrosine kinase, catalytic domain / Tyrosine protein kinases specific active-site signature. / Tyrosine-protein kinase, active site / PH-like domain superfamily / Serine-threonine/tyrosine-protein kinase, catalytic domain / Protein tyrosine and serine/threonine kinase / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily

Component:

Tyrosine-protein kinase BTK

• Biological species: Homo sapiens (human)

Contact author

• Daniel Duarte (EPFL)

Models

• Model #2785: ; Type: dummy / Radius of dummy atoms: 1.70 A / Symmetry: P1 / Chi-square value: 1.002 / P-value: 0.092064; Search similar-shape structures of this assembly by Omokage search (details)
• Model #2787: ; Type: atomic / Chi-square value: 1.275; Search similar-shape structures of this assembly by Omokage search (details)

Sample

• Sample: Name: Bruton's Tyrosine Kinase - kinase domain / Specimen concentration: 0.91-4.15
• Buffer: Name: 20mM Tris, 150mM NaCl, 1mM TCEP, 5% glycerol / pH: 7.5

Entity #1452

Name: Btk-KD / Type: protein / Description: Bruton's tyrosine kinase, kinase domain / Formula weight: 31.793 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: Q06187
Sequence:

GAMGSGYGSW EIDPKDLTFL KELGTGQFGV VKYGKWRGQY DVAIKMIKEG SMSEDEFIEE AKVMMNLSHE KLVQLYGVCT KQRPIFIITE YMANGCLLNY LREMRHRFQT QQLLEMCKDV CEAMEYLESK QFLHRDLAAR NCLVNDQGVV KVSDFGLSRY VLDDEYTSSV GSKFPVRWSP PEVLMYSKFS SKSDIWAFGV LMWEIYSLGK MPYERFTNSE TAEHIAQGLR LYRPHLASEK VYTIMYSCWH EKADERPTFK ILLSNILDVM DEES

Experimental information

• Beam: Instrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.09919 Å / Dist. spec. to detc.: 2.869 mm
• Detector: Name: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm

Scan

Title: Bruton's Tyrosine Kinase - kinase domain / Measurement date: May 11, 2018 / Storage temperature: 10 °C / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 10 / Unit: 1/nm /

	Min	Max
Q	0.0582	4.9277

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 800 /

	Min	Max
Q	0.0675539	3.81949
P(R) point	1	800
R	0	6.75

Result

Type of curve: merged /

	Experimental	Porod
MW	28.9 kDa	30 kDa
Volume	-	52.07 nm3

	P(R)	Guinier	Guinier error
Forward scattering, I0	24.94	24.98	0.034
Radius of gyration, Rg	2.096 nm	2.09 nm	0.02

	Min	Max
D	-	6.75
Guinier point	3	120