[English] 日本語
Yorodumi- SASDDY4: Protein sex-lethal mutant with 10GS-linker (Protein sex-lethal mu... -
+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDDY4 |
---|---|
Sample | Protein sex-lethal mutant with 10GS-linker
|
Function / homology | Function and homology information sex determination, primary response to X:A ratio / germarium-derived cystoblast division / epithelium regeneration / female sex determination / somatic sex determination / imaginal disc growth / female germ-line sex determination / oocyte differentiation / sex determination / regulation of stem cell division ...sex determination, primary response to X:A ratio / germarium-derived cystoblast division / epithelium regeneration / female sex determination / somatic sex determination / imaginal disc growth / female germ-line sex determination / oocyte differentiation / sex determination / regulation of stem cell division / negative regulation of RNA export from nucleus / poly-pyrimidine tract binding / sex-chromosome dosage compensation / sex differentiation / alternative mRNA splicing, via spliceosome / negative regulation of receptor signaling pathway via JAK-STAT / poly(A) binding / positive regulation of smoothened signaling pathway / pre-mRNA binding / regulation of mRNA splicing, via spliceosome / reciprocal meiotic recombination / poly(U) RNA binding / regulation of alternative mRNA splicing, via spliceosome / oogenesis / negative regulation of mRNA splicing, via spliceosome / negative regulation of translational initiation / mRNA regulatory element binding translation repressor activity / positive regulation of RNA splicing / mRNA 3'-UTR binding / mRNA 5'-UTR binding / negative regulation of translation / protein stabilization / ribonucleoprotein complex / mRNA binding / protein-containing complex / RNA binding / nucleus / cytosol / cytoplasm Similarity search - Function |
Biological species | Drosophila melanogaster (fruit fly) |
Citation | Journal: ACS Comb Sci / Year: 2018 Title: A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions. Authors: Po-Chia Chen / Pawel Masiewicz / Vladimir Rybin / Dmitri Svergun / Janosch Hennig / Abstract: We present a screening protocol utilizing small-angle X-ray scattering (SAXS) to obtain structural information on biomolecular interactions independent of prior knowledge, so as to complement ...We present a screening protocol utilizing small-angle X-ray scattering (SAXS) to obtain structural information on biomolecular interactions independent of prior knowledge, so as to complement affinity-based screening and provide leads for further exploration. This protocol categorizes ligand titrations by computing pairwise agreement between curves, and separately estimates affinities by quantifying complex formation as a departure from the linear sum properties of solution SAXS. The protocol is validated by sparse sequence search around the native poly uridine RNA motifs of the two-RRM domain Sex-lethal protein (Sxl). The screening of 35 RNA motifs between 4 to 10 nucleotides reveals a strong variation of resulting complexes, revealed to be preference-switching between 1:1 and 2:2 binding stoichiometries upon addition of structural modeling. Validation of select sequences in isothermal calorimetry and NMR titration retrieves domain-specific roles and function of a guanine anchor. These findings reinforce the suitability of SAXS as a complement in lead identification. |
Contact author |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Models
Model #1866 | Type: mix / Software: (2.1) / Radius of dummy atoms: 1.90 A / Chi-square value: 0.679 / P-value: 0.675072 Search similar-shape structures of this assembly by Omokage search (details) |
---|---|
Model #1867 | Type: mix / Software: (2.1) / Radius of dummy atoms: 1.90 A / Chi-square value: 0.679 / P-value: 0.675072 Search similar-shape structures of this assembly by Omokage search (details) |
Model #1868 | Type: mix / Software: (2.1) / Radius of dummy atoms: 1.90 A / Chi-square value: 0.679 / P-value: 0.675072 Search similar-shape structures of this assembly by Omokage search (details) |
Model #1869 | Type: mix / Software: (2.1) / Radius of dummy atoms: 1.90 A / Chi-square value: 0.679 / P-value: 0.675072 Search similar-shape structures of this assembly by Omokage search (details) |
Model #1870 | Type: mix / Software: (2.1) / Radius of dummy atoms: 1.90 A / Chi-square value: 0.679 / P-value: 0.675072 Search similar-shape structures of this assembly by Omokage search (details) |
-Sample
Sample | Name: Protein sex-lethal mutant with 10GS-linker / Specimen concentration: 1 mg/ml |
---|---|
Buffer | Name: 10 mM KP, 50 mM NaCl, 10 mM DTT / pH: 6 |
Entity #1006 | Name: Sxl10GS / Type: protein / Description: Protein sex-lethal mutant / Formula weight: 20.432 / Num. of mol.: 1 / Source: Drosophila melanogaster / References: UniProt: P19339 Sequence: GAMASNTNLI VNYLPQDMTD RELYALFRAI GPINTCRIMR DYKTGYSFGY AFVDFTSEMD SQRAIKVLNG ITVRNKRLKV SYARPGGGSG SGGGGSGESI KDTNLYVTNL PRTITDDQLD TIFGKYGSIV QKNILRDKLT GRPRGVAFVR YNKREEAQEA ISALNNVIPE GGSQPLSVRL AEEHGK |
-Experimental information
Beam | Instrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.09919 Å / Dist. spec. to detc.: 2.867 mm | |||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Detector | Name: Pilatus 1M | |||||||||||||||||||||||||||||||||
Scan | Title: Protein sex-lethal mutant with 10GS-linker / Measurement date: Feb 2, 2017 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 10 / Unit: 1/nm /
| |||||||||||||||||||||||||||||||||
Distance distribution function P(R) | Sofotware P(R): GNOM 4.5a / Number of points: 297 /
| |||||||||||||||||||||||||||||||||
Result | Type of curve: single_conc /
|