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- SASDDY4: Protein sex-lethal mutant with 10GS-linker (Protein sex-lethal mu... -
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Open data
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Basic information
Entry | Database: SASBDB / ID: SASDDY4 |
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![]() | Protein sex-lethal mutant with 10GS-linker
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Function / homology | ![]() sex determination, primary response to X:A ratio / germarium-derived cystoblast division / epithelium regeneration / female sex determination / somatic sex determination / female germ-line sex determination / oocyte differentiation / imaginal disc growth / negative regulation of RNA export from nucleus / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function |
Biological species | ![]() ![]() ![]() |
![]() | ![]() Title: A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions. Authors: Po-Chia Chen / Pawel Masiewicz / Vladimir Rybin / Dmitri Svergun / Janosch Hennig / ![]() Abstract: We present a screening protocol utilizing small-angle X-ray scattering (SAXS) to obtain structural information on biomolecular interactions independent of prior knowledge, so as to complement ...We present a screening protocol utilizing small-angle X-ray scattering (SAXS) to obtain structural information on biomolecular interactions independent of prior knowledge, so as to complement affinity-based screening and provide leads for further exploration. This protocol categorizes ligand titrations by computing pairwise agreement between curves, and separately estimates affinities by quantifying complex formation as a departure from the linear sum properties of solution SAXS. The protocol is validated by sparse sequence search around the native poly uridine RNA motifs of the two-RRM domain Sex-lethal protein (Sxl). The screening of 35 RNA motifs between 4 to 10 nucleotides reveals a strong variation of resulting complexes, revealed to be preference-switching between 1:1 and 2:2 binding stoichiometries upon addition of structural modeling. Validation of select sequences in isothermal calorimetry and NMR titration retrieves domain-specific roles and function of a guanine anchor. These findings reinforce the suitability of SAXS as a complement in lead identification. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
-Models
Model #1866 | ![]() Type: mix / Software: (2.1) / Radius of dummy atoms: 1.90 A / Chi-square value: 0.679 / P-value: 0.675072 ![]() |
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Model #1867 | ![]() Type: mix / Software: (2.1) / Radius of dummy atoms: 1.90 A / Chi-square value: 0.679 / P-value: 0.675072 ![]() |
Model #1868 | ![]() Type: mix / Software: (2.1) / Radius of dummy atoms: 1.90 A / Chi-square value: 0.679 / P-value: 0.675072 ![]() |
Model #1869 | ![]() Type: mix / Software: (2.1) / Radius of dummy atoms: 1.90 A / Chi-square value: 0.679 / P-value: 0.675072 ![]() |
Model #1870 | ![]() Type: mix / Software: (2.1) / Radius of dummy atoms: 1.90 A / Chi-square value: 0.679 / P-value: 0.675072 ![]() |
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Sample
![]() | Name: Protein sex-lethal mutant with 10GS-linker / Specimen concentration: 1 mg/ml |
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Buffer | Name: 10 mM KP, 50 mM NaCl, 10 mM DTT / pH: 6 |
Entity #1006 | Name: Sxl10GS / Type: protein / Description: Protein sex-lethal mutant / Formula weight: 20.432 / Num. of mol.: 1 / Source: Drosophila melanogaster / References: UniProt: P19339 Sequence: GAMASNTNLI VNYLPQDMTD RELYALFRAI GPINTCRIMR DYKTGYSFGY AFVDFTSEMD SQRAIKVLNG ITVRNKRLKV SYARPGGGSG SGGGGSGESI KDTNLYVTNL PRTITDDQLD TIFGKYGSIV QKNILRDKLT GRPRGVAFVR YNKREEAQEA ISALNNVIPE GGSQPLSVRL AEEHGK |
-Experimental information
Beam | Instrument name: ESRF BM29 / City: Grenoble / 国: France ![]() ![]() | |||||||||||||||||||||||||||||||||
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Detector | Name: Pilatus 1M | |||||||||||||||||||||||||||||||||
Scan |
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Distance distribution function P(R) |
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Result |
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