+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDCZ4 |
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Sample | RNase E 603-850/ATP-dependent RNA helicase (RhlB) binary complex
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Function / homology | Function and homology information : / RNA catabolic process / RNA helicase activity / RNA helicase / RNA binding / ATP binding / cytoplasm Similarity search - Function |
Biological species | Escherichia coli (E. coli) |
Citation | Date: 2017 Nov 9 Title: Analysis of the natively unstructured RNA/protein-recognition core in the Escherichia coli RNA degradosome and its interactions with regulatory RNA/Hfq complexes Authors: Bruce H / Du D / Matak-Vinkovic D / Bandyra K / Broadhurst R / Martin E / Sobott F / Shkumatov A |
Contact author |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Models
Model #1313 | Type: dummy / Software: (2.3i r9450) / Radius of dummy atoms: 1.90 A / Symmetry: P1 Comment: Best fitting model out of 10 independent reconstructions Chi-square value: 16.39 / P-value: 0.000001 Search similar-shape structures of this assembly by Omokage search (details) |
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-Sample
Sample | Name: RNase E 603-850/ATP-dependent RNA helicase (RhlB) binary complex Specimen concentration: 22.3 mg/ml / Entity id: 697 / 698 |
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Buffer | Name: 50 mM Tris HCl, 100 mM NaCl, 100 mM KCl, 10 mM MgCl2, 10 mM DTT and 5 % glycerol (v/v) pH: 7.5 |
Entity #697 | Name: RNase E 603-850 / Type: protein / Description: RNase E 603-850 / Formula weight: 30.127 / Num. of mol.: 1 / Source: Escherichia coli / References: UniProt: Q46977 Sequence: ERQQDRRKPR QNNRRDRNER RDTRSERTEG SDNREENRRN RRQAQQQTAE TRESRQQAEV TEKARTADEQ QAPRRERSRR RNDDKRQAQQ EAKALNVEEQ SVQETEQEER VRPVQPRRKQ RQLNQKVRYE QSVAEEAVVA PVVEETVAAE PIVQEAPAPR TELVKVPLPV ...Sequence: ERQQDRRKPR QNNRRDRNER RDTRSERTEG SDNREENRRN RRQAQQQTAE TRESRQQAEV TEKARTADEQ QAPRRERSRR RNDDKRQAQQ EAKALNVEEQ SVQETEQEER VRPVQPRRKQ RQLNQKVRYE QSVAEEAVVA PVVEETVAAE PIVQEAPAPR TELVKVPLPV VAQTAPEQQE ENNADNRDNG GMPRRSRRSP RHLRVSGQRR RRYRDERYPT QSPMPLTVAC ASPELASGKV WIRYPIVRHH HHHH |
Entity #698 | Name: RhlB / Type: protein / Description: ATP-dependent RNA helicase RhlB / Formula weight: 47.125 / Num. of mol.: 1 / Source: Escherichia coli / References: UniProt: W8ZZD4 Sequence: MSKTHLTEQK FSDFALHPKV VEALEKKGFH NCTPIQALAL PLTLAGRDVA GQAQTGTGKT MAFLTSTFHY LLSHPAIADR KVNQPRALIM APTRELAVQI HADAEPLAEA TGLKLGLAYG GDGYDKQLKV LESGVDILIG TTGRLIDYAK QNHINLGAIQ VVVLDEADRM ...Sequence: MSKTHLTEQK FSDFALHPKV VEALEKKGFH NCTPIQALAL PLTLAGRDVA GQAQTGTGKT MAFLTSTFHY LLSHPAIADR KVNQPRALIM APTRELAVQI HADAEPLAEA TGLKLGLAYG GDGYDKQLKV LESGVDILIG TTGRLIDYAK QNHINLGAIQ VVVLDEADRM YDLGFIKDIR WLFRRMPPAN QRLNMLFSAT LSYRVRELAF EQMNNAEYIE VEPEQKTGHR IKEELFYPSN EEKMRLLQTL IEEEWPDRAI IFANTKHRCE EIWGHLAADG HRVGLLTGDV AQKKRLRILD EFTRGDLDIL VATDVAARGL HIPAVTHVFN YDLPDDCEDY VHRIGRTGRA GASGHSISLA CEEYALNLPA IETYIGHSIP VSKYNPDALM TDLPKPLRLT RPRTGNGPRR TGAPRNRRRS G |
-Experimental information
Beam | Instrument name: SOLEIL SWING / City: Saint-Aubin / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.1022 Å / Dist. spec. to detc.: 1.79 mm | ||||||||||||||||||||||||||||||||||||
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Detector | Name: AVIEX / Type: CCD | ||||||||||||||||||||||||||||||||||||
Scan | Title: RNase E 603-850/ATP-dependent RNA helicase (RhlB) binary complex Measurement date: Feb 11, 2016 / Storage temperature: 15 °C / Cell temperature: 15 °C / Exposure time: 1.5 sec. / Number of frames: 250 / Unit: 1/A /
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Distance distribution function P(R) | Sofotware P(R): GNOM 5.0 / Number of points: 875 /
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Result | Type of curve: other /
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