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Open data
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Basic information
| Entry | Database: PDB / ID: 9ygv | |||||||||
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| Title | Photosystem I FCP Supercomplex from Macrocystis pyrifera | |||||||||
Components |
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Keywords | PHOTOSYNTHESIS / Photosystem I / Brown algae / Macrocystis pyrifera | |||||||||
| Function / homology | Function and homology informationplastid thylakoid membrane / thylakoid membrane / photosystem I reaction center / photosystem I / photosynthetic electron transport in photosystem I / photosystem I / chlorophyll binding / chloroplast thylakoid membrane / photosynthesis / 4 iron, 4 sulfur cluster binding ...plastid thylakoid membrane / thylakoid membrane / photosystem I reaction center / photosystem I / photosynthetic electron transport in photosystem I / photosystem I / chlorophyll binding / chloroplast thylakoid membrane / photosynthesis / 4 iron, 4 sulfur cluster binding / electron transfer activity / oxidoreductase activity / magnesium ion binding / metal ion binding Similarity search - Function | |||||||||
| Biological species | Macrocystis pyrifera (giant kelp) | |||||||||
| Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.47 Å | |||||||||
Authors | Maturana, P. / Maldonado, M. | |||||||||
| Funding support | United States, 2items
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Citation | Journal: To Be PublishedTitle: Structure of a Photosystem-I-FCP supercomplex from brown alga Macrocystis pyrifera (giant kelp) Authors: Weissman, J.D. / Maturana, P. / Riddle, R. / Wyatt, G. / Dubinin, V.G.T. / Zerbe, P. / Maldonado, M. | |||||||||
| History |
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Structure visualization
| Structure viewer | Molecule: Molmil Jmol/JSmol |
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Downloads & links
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Download
| PDBx/mmCIF format | 9ygv.cif.gz | 1.3 MB | Display | PDBx/mmCIF format |
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| PDB format | pdb9ygv.ent.gz | Display | PDB format | |
| PDBx/mmJSON format | 9ygv.json.gz | Tree view | PDBx/mmJSON format | |
| Others | Other downloads |
-Validation report
| Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/yg/9ygv ftp://data.pdbj.org/pub/pdb/validation_reports/yg/9ygv | HTTPS FTP |
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-Related structure data
| Related structure data | ![]() 72770MC M: map data used to model this data C: citing same article ( |
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| Similar structure data | Similarity search - Function & homology F&H Search |
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Links
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Assembly
| Deposited unit | ![]()
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Components
-Protein , 18 types, 20 molecules 13578CRb610111391516171924a
| #1: Protein | Mass: 24077.623 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Fucoxanthin-chlorophyll a-c binding protein for position 1 in PSI-FCP supercomplex from Macrocystis pyrifera Source: (natural) Macrocystis pyrifera (giant kelp) | ||||||||||||||||||
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| #2: Protein | Mass: 20481.303 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Fucoxanthin-chlorophyll a-c binding protein for position 3 in PSI-FCP supercomplex from Macrocystis pyrifera Source: (natural) Macrocystis pyrifera (giant kelp) | ||||||||||||||||||
| #3: Protein | Mass: 23108.686 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Fucoxanthin-chlorophyll a-c binding protein for position 5 in PSI-FCP supercomplex from Macrocystis pyrifera Source: (natural) Macrocystis pyrifera (giant kelp) | ||||||||||||||||||
| #4: Protein | Mass: 18366.344 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Fucoxanthin-chlorophyll a-c binding protein for position 7 in PSI-FCP supercomplex from Macrocystis pyrifera Source: (natural) Macrocystis pyrifera (giant kelp) | ||||||||||||||||||
| #5: Protein | Mass: 22788.162 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Fucoxanthin-chlorophyll a-c binding protein for position 8 in PSI-FCP supercomplex from Macrocystis pyrifera Source: (natural) Macrocystis pyrifera (giant kelp) | ||||||||||||||||||
| #8: Protein | Mass: 8780.103 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: Photosystem I iron-sulfur center subunit VII / Source: (natural) Macrocystis pyrifera (giant kelp) / References: UniProt: A0A8F0F8D0, photosystem I | ||||||||||||||||||
| #15: Protein | Mass: 13673.047 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Macrocystis pyrifera (giant kelp) | ||||||||||||||||||
| #16: Protein | Mass: 12017.806 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Fucoxanthin-chlorophyll a-c binding protein for position B in PSI-FCP supercomplex from Macrocystis pyrifera Source: (natural) Macrocystis pyrifera (giant kelp) | ||||||||||||||||||
| #17: Protein | Mass: 22069.484 Da / Num. of mol.: 2 / Source method: isolated from a natural source Details: Fucoxanthin-chlorophyll a-c binding protein for position 6 and 10 in PSI-FCP supercomplex from Macrocystis pyrifera Source: (natural) Macrocystis pyrifera (giant kelp)#18: Protein | | Mass: 22865.518 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Fucoxanthin-chlorophyll a-c binding protein for position 11 in PSI-FCP supercomplex from Macrocystis pyrifera Source: (natural) Macrocystis pyrifera (giant kelp)#19: Protein | Mass: 23239.904 Da / Num. of mol.: 2 / Source method: isolated from a natural source Details: Fucoxanthin-chlorophyll a-c binding protein for position 9 and 13 in PSI-FCP supercomplex from Macrocystis pyrifera Source: (natural) Macrocystis pyrifera (giant kelp)#20: Protein | | Mass: 13209.274 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Fucoxanthin-chlorophyll a-c binding protein for position 15 in PSI-FCP supercomplex from Macrocystis pyrifera Source: (natural) Macrocystis pyrifera (giant kelp) / References: photosystem I#21: Protein | | Mass: 13890.110 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Fucoxanthin-chlorophyll a-c binding protein for position 16 in PSI-FCP supercomplex from Macrocystis pyrifera Source: (natural) Macrocystis pyrifera (giant kelp) / References: photosystem I#22: Protein | | Mass: 22960.195 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Fucoxanthin-chlorophyll a-c binding protein for position 17 in PSI-FCP supercomplex from Macrocystis pyrifera Source: (natural) Macrocystis pyrifera (giant kelp)#23: Protein | | Mass: 12783.750 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Fucoxanthin-chlorophyll a-c binding protein for position 19 in PSI-FCP supercomplex from Macrocystis pyrifera Source: (natural) Macrocystis pyrifera (giant kelp) / References: photosystem I#24: Protein | | Mass: 8868.924 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Fucoxanthin-chlorophyll a-c binding protein for position 2 in PSI-FCP supercomplex from Macrocystis pyrifera Source: (natural) Macrocystis pyrifera (giant kelp)#25: Protein | | Mass: 24775.438 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Fucoxanthin-chlorophyll a-c binding protein for position 4 in PSI-FCP supercomplex from Macrocystis pyrifera Source: (natural) Macrocystis pyrifera (giant kelp)#27: Protein | | Mass: 12868.854 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Fucoxanthin-chlorophyll a-c binding protein for position A in PSI-FCP supercomplex from Macrocystis pyrifera Source: (natural) Macrocystis pyrifera (giant kelp) |
-Photosystem I P700 chlorophyll a apoprotein ... , 2 types, 2 molecules AB
| #6: Protein | Mass: 83161.633 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: Photosystem I P700 chlorophyll a apoprotein A1 / Source: (natural) Macrocystis pyrifera (giant kelp) / References: UniProt: A0A8F0JYL2, photosystem I |
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| #7: Protein | Mass: 82548.211 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: Photosystem I P700 chlorophyll a apoprotein A2 / Source: (natural) Macrocystis pyrifera (giant kelp) / References: UniProt: A0A8F0F8K8, photosystem I |
-Photosystem I reaction center subunit ... , 7 types, 7 molecules DEFIJML
| #9: Protein | Mass: 15029.392 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: Photosystem I reaction center subunit II / Source: (natural) Macrocystis pyrifera (giant kelp) / References: UniProt: A0A8F0F8I9 |
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| #10: Protein | Mass: 6962.011 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: Photosystem I reaction center subunit IV / Source: (natural) Macrocystis pyrifera (giant kelp) / References: UniProt: A0A8F0F8D1 |
| #11: Protein | Mass: 22774.436 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: Photosystem I reaction center subunit III / Source: (natural) Macrocystis pyrifera (giant kelp) / References: UniProt: A0A8F0JY91 |
| #12: Protein/peptide | Mass: 4007.902 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: Photosystem I reaction center subunit VIII / Source: (natural) Macrocystis pyrifera (giant kelp) / References: UniProt: A0A8F0F8C2 |
| #13: Protein/peptide | Mass: 4854.640 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: Photosystem I reaction center subunit IX / Source: (natural) Macrocystis pyrifera (giant kelp) / References: UniProt: A0A8F0F8B4 |
| #14: Protein/peptide | Mass: 3247.974 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: Photosystem I reaction center subunit XII / Source: (natural) Macrocystis pyrifera (giant kelp) / References: UniProt: A0A8F0JYJ8 |
| #26: Protein | Mass: 15699.177 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: Photosystem I reaction center subunit XI / Source: (natural) Macrocystis pyrifera (giant kelp) / References: UniProt: A0A8F0F8N6 |
-Sugars , 1 types, 3 molecules 
| #34: Sugar |
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-Non-polymers , 9 types, 315 molecules 
















| #28: Chemical | ChemComp-CLA / #29: Chemical | ChemComp-A86 / ( #30: Chemical | ChemComp-KC1 / #31: Chemical | ChemComp-LHG / #32: Chemical | ChemComp-XAT / ( #33: Chemical | ChemComp-BCR / #35: Chemical | #36: Chemical | #37: Chemical | ChemComp-LMG / | |
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-Details
| Has ligand of interest | N |
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| Has protein modification | Y |
-Experimental details
-Experiment
| Experiment | Method: ELECTRON MICROSCOPY |
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| EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
| Component | Name: Supercomplex of Macrocystis pyrifera photosystem I and FCP antenna Type: COMPLEX Entity ID: #1, #17-#24, #2, #25, #3-#13, #26, #14-#15, #27, #16 Source: NATURAL | ||||||||||||||||||||||||||||||
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| Source (natural) | Organism: Macrocystis pyrifera (giant kelp) | ||||||||||||||||||||||||||||||
| Buffer solution | pH: 7.5 Details: 15 mM HEPES, 20 mM KCl, and 5 mM NaCl, 0.01 % GDN, 0.1% Digitonin, pH 7.5. | ||||||||||||||||||||||||||||||
| Buffer component |
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| Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES | ||||||||||||||||||||||||||||||
| Specimen support | Grid material: GOLD / Grid mesh size: 300 divisions/in. / Grid type: Quantifoil R1.2/1.3 | ||||||||||||||||||||||||||||||
| Vitrification | Instrument: LEICA EM GP / Cryogen name: ETHANE / Humidity: 90 % / Chamber temperature: 291.15 K |
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Electron microscopy imaging
| Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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| Microscopy | Model: TFS KRIOS |
| Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
| Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 1000 nm / Cs: 2.7 mm |
| Image recording | Average exposure time: 4.95 sec. / Electron dose: 50 e/Å2 / Film or detector model: TFS FALCON 4i (4k x 4k) / Num. of grids imaged: 1 / Num. of real images: 15690 |
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Processing
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| CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
| 3D reconstruction | Resolution: 2.47 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 133000 / Num. of class averages: 1 / Symmetry type: POINT | ||||||||||||||||||||||||
| Refinement | Highest resolution: 2.47 Å Stereochemistry target values: REAL-SPACE (WEIGHTED MAP SUM AT ATOM CENTERS) | ||||||||||||||||||||||||
| Refine LS restraints |
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About Yorodumi




Macrocystis pyrifera (giant kelp)
United States, 2items
Citation
PDBj











FIELD EMISSION GUN