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Yorodumi- PDB-9vtg: cryoEM structure of tobacco mosaic virus tRNA-like structure comp... -
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Open data
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Basic information
| Entry | Database: PDB / ID: 9vtg | |||||||||||||||
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| Title | cryoEM structure of tobacco mosaic virus tRNA-like structure complexed with tobacco 60S | |||||||||||||||
Components |
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Keywords | RIBOSOME / tRNA-like structure / tobacco / Nicotiana benthamiana / tobacco mosaic virus / RNA structure / translation | |||||||||||||||
| Function / homology | Function and homology informationadaxial/abaxial pattern specification / leaf morphogenesis / response to high light intensity / response to UV-B / protein-RNA complex assembly / ribosomal large subunit biogenesis / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosome biogenesis / 5S rRNA binding / ribosomal large subunit assembly ...adaxial/abaxial pattern specification / leaf morphogenesis / response to high light intensity / response to UV-B / protein-RNA complex assembly / ribosomal large subunit biogenesis / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosome biogenesis / 5S rRNA binding / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / cell division / mRNA binding / RNA binding / zinc ion binding / nucleus / cytoplasm Similarity search - Function | |||||||||||||||
| Biological species | ![]() synthetic construct (others) | |||||||||||||||
| Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.8 Å | |||||||||||||||
Authors | Lu, G. / Lin, J. | |||||||||||||||
| Funding support | China, 2items
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Citation | Journal: To be publishedTitle: cryoEM structure of tobacco mosaic virus tRNA-like structure complexed with tobacco 60S Authors: Lu, G. / Lin, J. | |||||||||||||||
| History |
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Structure visualization
| Structure viewer | Molecule: Molmil Jmol/JSmol |
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Downloads & links
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Download
| PDBx/mmCIF format | 9vtg.cif.gz | 3.2 MB | Display | PDBx/mmCIF format |
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| PDB format | pdb9vtg.ent.gz | Display | PDB format | |
| PDBx/mmJSON format | 9vtg.json.gz | Tree view | PDBx/mmJSON format | |
| Others | Other downloads |
-Validation report
| Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/vt/9vtg ftp://data.pdbj.org/pub/pdb/validation_reports/vt/9vtg | HTTPS FTP |
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-Related structure data
| Related structure data | ![]() 65327MC M: map data used to model this data C: citing same article ( |
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| Similar structure data | Similarity search - Function & homology F&H Search |
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Links
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Assembly
| Deposited unit | ![]()
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Components
-Protein , 2 types, 2 molecules LALn
| #1: Protein | Mass: 24778.387 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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| #40: Protein | Mass: 14703.358 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
+60S ribosomal protein ... , 34 types, 34 molecules LBLCLDLELFLGLHLILJLKLLLNLOLPLQLRLSLTLVLXLYLaLbLcLdLeLfLgLhLj...
-Large ribosomal subunit protein ... , 3 types, 3 molecules LMLZLi
| #13: Protein | Mass: 17355.531 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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| #26: Protein | Mass: 12506.270 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
| #35: Protein | Mass: 13788.358 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
-Ribosomal protein ... , 3 types, 3 molecules LULWLk
| #21: Protein | Mass: 24226.291 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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| #23: Protein | Mass: 24742.324 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
| #37: Protein | Mass: 10879.783 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
-RNA chain , 4 types, 4 molecules 10121314
| #43: RNA chain | Mass: 65633.805 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
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| #44: RNA chain | Mass: 38328.777 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
| #45: RNA chain | Mass: 52534.219 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
| #46: RNA chain | Mass: 1097118.125 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
-Details
| Has protein modification | N |
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-Experimental details
-Experiment
| Experiment | Method: ELECTRON MICROSCOPY |
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| EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
| Component | Name: tobacco 80S ribosome with tRNA-like structure / Type: RIBOSOME / Entity ID: all / Source: NATURAL |
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| Source (natural) | Organism: ![]() |
| Buffer solution | pH: 7.4 |
| Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
| Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
| Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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| Microscopy | Model: TFS KRIOS |
| Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: OTHER |
| Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2500 nm / Nominal defocus min: 1500 nm |
| Image recording | Electron dose: 50 e/Å2 / Film or detector model: FEI FALCON I (4k x 4k) |
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Processing
| EM software |
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| CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | |||||||||
| 3D reconstruction | Resolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 53930 / Symmetry type: POINT |
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FIELD EMISSION GUN