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- PDB-9uv7: Single-subunit apo Escherichia coli nicotinamide nucleotide trans... -

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Basic information

Entry
Database: PDB / ID: 9uv7
TitleSingle-subunit apo Escherichia coli nicotinamide nucleotide transhydrogenase
Components
  • (NAD(P) transhydrogenase subunit alpha) x 2
  • NAD(P) transhydrogenase subunit beta
KeywordsMEMBRANE PROTEIN / Nicotinamide nucleotide transhydrogenase / Mitochondrial inner membrane / E. coli transhydrogenase / Hydride transfer / Proton motive force / NAD+ / NADH / NADP+ / NADPH
Function / homology
Function and homology information


proton export across plasma membrane / proton-translocating NAD(P)+ transhydrogenase activity / proton-translocating NAD(P)+ transhydrogenase / NADPH regeneration / NADP binding / protein dimerization activity / oxidoreductase activity / plasma membrane
Similarity search - Function
NAD(P) transhydrogenase, alpha subunit / NAD(P) transhydrogenase, alpha subunit, C-terminal / 4TM region of pyridine nucleotide transhydrogenase, mitoch / NADP transhydrogenase, beta subunit / NADP transhydrogenase beta-like domain / NAD(P) transhydrogenase beta subunit / Alanine dehydrogenase/NAD(P) transhydrogenase, conserved site-1 / Alanine dehydrogenase & pyridine nucleotide transhydrogenase signature 1. / Alanine dehydrogenase/pyridine nucleotide transhydrogenase, conserved site-2 / Alanine dehydrogenase & pyridine nucleotide transhydrogenase signature 2. ...NAD(P) transhydrogenase, alpha subunit / NAD(P) transhydrogenase, alpha subunit, C-terminal / 4TM region of pyridine nucleotide transhydrogenase, mitoch / NADP transhydrogenase, beta subunit / NADP transhydrogenase beta-like domain / NAD(P) transhydrogenase beta subunit / Alanine dehydrogenase/NAD(P) transhydrogenase, conserved site-1 / Alanine dehydrogenase & pyridine nucleotide transhydrogenase signature 1. / Alanine dehydrogenase/pyridine nucleotide transhydrogenase, conserved site-2 / Alanine dehydrogenase & pyridine nucleotide transhydrogenase signature 2. / Alanine dehydrogenase/PNT, C-terminal domain / Alanine dehydrogenase/pyridine nucleotide transhydrogenase, N-terminal / Alanine dehydrogenase/PNT, N-terminal domain / Alanine dehydrogenase/PNT, C-terminal domain / Alanine dehydrogenase/PNT, N-terminal domain / Alanine dehydrogenase/pyridine nucleotide transhydrogenase, NAD(H)-binding domain / DHS-like NAD/FAD-binding domain superfamily / NAD(P)-binding domain superfamily
Similarity search - Domain/homology
NAD(P) transhydrogenase subunit alpha / NAD(P) transhydrogenase subunit beta
Similarity search - Component
Biological speciesEscherichia coli K-12 (bacteria)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.43 Å
AuthorsZhu, J. / Gennis, R.B. / Zhang, K. / Li, J.
Funding support China, 1items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)32201038 China
CitationJournal: To Be Published
Title: Single-subunit apo Escherichia coli nicotinamide nucleotide transhydrogenase
Authors: Zhu, J. / Gennis, R.B. / Zhang, K. / Li, J.
History
DepositionMay 9, 2025Deposition site: PDBJ / Processing site: PDBC
Revision 1.0May 27, 2026Provider: repository / Type: Initial release
Revision 1.0May 27, 2026Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release
Revision 1.0May 27, 2026Data content type: Image / Data content type: Image / Provider: repository / Type: Initial release
Revision 1.0May 27, 2026Data content type: Primary map / Data content type: Primary map / Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
B: NAD(P) transhydrogenase subunit beta
A: NAD(P) transhydrogenase subunit beta
C: NAD(P) transhydrogenase subunit alpha
D: NAD(P) transhydrogenase subunit alpha
E: NAD(P) transhydrogenase subunit alpha
F: NAD(P) transhydrogenase subunit alpha


Theoretical massNumber of molelcules
Total (without water)199,3796
Polymers199,3796
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

#1: Protein NAD(P) transhydrogenase subunit beta / Nicotinamide nucleotide transhydrogenase subunit beta / Pyridine nucleotide transhydrogenase subunit beta


Mass: 48762.746 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli K-12 (bacteria) / Strain: K-12 / Gene: pntB, b1602, JW1594 / Production host: Escherichia coli (E. coli)
References: UniProt: P0AB67, proton-translocating NAD(P)+ transhydrogenase
#2: Protein NAD(P) transhydrogenase subunit alpha / Nicotinamide nucleotide transhydrogenase subunit alpha / Pyridine nucleotide transhydrogenase subunit alpha


Mass: 10742.778 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli K-12 (bacteria) / Strain: K-12 / Gene: pntA, b1603, JW1595 / Production host: Escherichia coli (E. coli)
References: UniProt: P07001, proton-translocating NAD(P)+ transhydrogenase
#3: Protein NAD(P) transhydrogenase subunit alpha / Nicotinamide nucleotide transhydrogenase subunit alpha / Pyridine nucleotide transhydrogenase subunit alpha


Mass: 40183.902 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli K-12 (bacteria) / Strain: K-12 / Gene: pntA, b1603, JW1595 / Production host: Escherichia coli (E. coli)
References: UniProt: P07001, proton-translocating NAD(P)+ transhydrogenase
Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: The E. coli transhydrogenase complex. / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT
Source (natural)Organism: Escherichia coli K-12 (bacteria)
Source (recombinant)Organism: Escherichia coli (E. coli)
Buffer solutionpH: 8
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 1200 nm
Image recordingElectron dose: 1.25 e/Å2 / Film or detector model: GATAN K3 (6k x 4k)

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Processing

EM software
IDNameVersionCategory
1cryoSPARCparticle selection
2PHENIX1.20.1_4487:model refinement
13cryoSPARC3D reconstruction
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.43 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 42826 / Symmetry type: POINT
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.01213733
ELECTRON MICROSCOPYf_angle_d1.21618658
ELECTRON MICROSCOPYf_dihedral_angle_d5.3071871
ELECTRON MICROSCOPYf_chiral_restr0.0482248
ELECTRON MICROSCOPYf_plane_restr0.0072361

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