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- PDB-9tnb: GABA-A receptor a3b3g2 + a3NB77 + bicuculline -

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Basic information

Entry
Database: PDB / ID: 9tnb
TitleGABA-A receptor a3b3g2 + a3NB77 + bicuculline
Components
  • (Gamma-aminobutyric acid receptor subunit ...) x 2
  • GABA-A receptor beta-3-bril
  • a3NB77
KeywordsMEMBRANE PROTEIN / pLGIC / GABA / Neurotransmission
Function / homology
Function and homology information


benzodiazepine receptor activity / extrasynaptic signaling via GABA / cellular response to histamine / GABA receptor activation / negative regulation of synaptic transmission, GABAergic / GABA-gated chloride ion channel activity / GABA-A receptor complex / inhibitory synapse assembly / GABA-A receptor activity / synaptic transmission, GABAergic ...benzodiazepine receptor activity / extrasynaptic signaling via GABA / cellular response to histamine / GABA receptor activation / negative regulation of synaptic transmission, GABAergic / GABA-gated chloride ion channel activity / GABA-A receptor complex / inhibitory synapse assembly / GABA-A receptor activity / synaptic transmission, GABAergic / gamma-aminobutyric acid signaling pathway / postsynaptic specialization membrane / chloride channel activity / Signaling by ERBB4 / chloride channel complex / cytoplasmic vesicle membrane / dendrite membrane / chloride transmembrane transport / transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential / GABA-ergic synapse / dendritic spine / postsynaptic membrane / postsynapse / axon / plasma membrane
Similarity search - Function
Gamma-aminobutyric-acid A receptor, alpha 3 subunit / Gamma-aminobutyric-acid A receptor, gamma 2 subunit / Gamma-aminobutyric acid receptor subunit gamma-1/4 / Gamma-aminobutyric-acid A receptor, alpha subunit / : / Gamma-aminobutyric acid A receptor/Glycine receptor alpha / Neurotransmitter-gated ion-channel, conserved site / Neurotransmitter-gated ion-channels signature. / Neurotransmitter-gated ion-channel transmembrane domain / Neurotransmitter-gated ion-channel transmembrane region ...Gamma-aminobutyric-acid A receptor, alpha 3 subunit / Gamma-aminobutyric-acid A receptor, gamma 2 subunit / Gamma-aminobutyric acid receptor subunit gamma-1/4 / Gamma-aminobutyric-acid A receptor, alpha subunit / : / Gamma-aminobutyric acid A receptor/Glycine receptor alpha / Neurotransmitter-gated ion-channel, conserved site / Neurotransmitter-gated ion-channels signature. / Neurotransmitter-gated ion-channel transmembrane domain / Neurotransmitter-gated ion-channel transmembrane region / Neurotransmitter-gated ion-channel transmembrane domain superfamily / Neuronal acetylcholine receptor / Neurotransmitter-gated ion-channel / Neurotransmitter-gated ion-channel ligand-binding domain / Neurotransmitter-gated ion-channel ligand-binding domain superfamily / Neurotransmitter-gated ion-channel ligand binding domain
Similarity search - Domain/homology
bicuculline methochloride / Gamma-aminobutyric acid receptor subunit gamma-2 / Gamma-aminobutyric acid receptor subunit alpha-3
Similarity search - Component
Biological speciesHomo sapiens (human)
Lama glama (llama)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.8 Å
AuthorsShang, C. / Nestorow, S.A. / Miller, P.S.
Funding support United Kingdom, 1items
OrganizationGrant numberCountry
Biotechnology and Biological Sciences Research Council (BBSRC)BB/M024709/1 United Kingdom
CitationJournal: Sci Adv / Year: 2026
Title: Determining the molecular and physiological actions of subtype-selective nanobodies of GABA receptors.
Authors: Jose Enrique Gonzalez-Prada / Sulin Liu / Chuhan Shang / Chloe S Chernoff / Damian P Bright / Martin Mortensen / Charlotte F Jones / Stephanie Nestorow / Vikram Babu Kasaragod / Wan-Na Chen ...Authors: Jose Enrique Gonzalez-Prada / Sulin Liu / Chuhan Shang / Chloe S Chernoff / Damian P Bright / Martin Mortensen / Charlotte F Jones / Stephanie Nestorow / Vikram Babu Kasaragod / Wan-Na Chen / Saad Hannan / Jianchong Zhou / Alexander W E Dunn / Asma Soltani / Richard J Turner / Natasha M Duggan / Yin Yuan / Ayla A Wahid / Steven W Hardwick / Suzanne Scott / Dimitri Y Chirgadze / Els Pardon / Jan Steyaert / A Radu Aricescu / Ole Paulsen / David Belin / Trevor G Smart / Paul S Miller /
Abstract: γ-Aminobutyric acid type-A (GABA) receptors are the principal mediators of inhibitory neurotransmission in the human central nervous system. The α- and α-containing subtypes have tightly ...γ-Aminobutyric acid type-A (GABA) receptors are the principal mediators of inhibitory neurotransmission in the human central nervous system. The α- and α-containing subtypes have tightly controlled spatial expression profiles, which influence anxiety, nociception, epilepsy, and autism. α/α-Selective small molecules compromise on strength of effect (efficacy) to avoid off-subtype modulation. To break this pharmacological deadlock, we study here a panel of nanobodies (NBs) raised against α- and α-containing GABA receptors. We identify subtype selective silent binders, positive allosteric modulators (PAMs), and inhibitors. Cryo-electron microscopy structures explain the binding modes and molecular mechanisms of action of representative NBs. Modulators exhibit distinct synaptic and extrasynaptic functional profiles in brain slices and neuronal networks and can reduce anxiety in vivo. These selective and efficacious NBs (whether inhibitors or positive modulators) enable strong yet precise pharmacological control of α/α-containing subtypes to advance basic research and as potential therapeutic leads to treat neuropsychiatric disorders.
History
DepositionDec 15, 2025Deposition site: PDBE / Processing site: PDBE
Revision 1.0Aug 26, 2026Provider: repository / Type: Initial release
Revision 1.0Aug 26, 2026Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Gamma-aminobutyric acid receptor subunit alpha-3
B: GABA-A receptor beta-3-bril
C: Gamma-aminobutyric acid receptor subunit gamma-2
D: Gamma-aminobutyric acid receptor subunit alpha-3
E: GABA-A receptor beta-3-bril
F: a3NB77
G: a3NB77
hetero molecules


Theoretical massNumber of molelcules
Total (without water)267,07820
Polymers260,0977
Non-polymers6,98113
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

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Gamma-aminobutyric acid receptor subunit ... , 2 types, 3 molecules ADC

#1: Protein Gamma-aminobutyric acid receptor subunit alpha-3 / GABA(A) receptor subunit alpha-3 / GABAAR subunit alpha-3


Mass: 43439.820 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: Glvi linker sequence (SQPARAA). Region substituted: Arg337-Ser425,Glvi linker sequence (SQPARAA). Region substituted: Arg337-Ser425
Source: (gene. exp.) Homo sapiens (human) / Gene: GABRA3 / Production host: Homo sapiens (human) / References: UniProt: P34903
#3: Protein Gamma-aminobutyric acid receptor subunit gamma-2 / GABA(A) receptor subunit gamma-2 / GABAAR subunit gamma-2


Mass: 42798.875 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Includes an 1D4 tag at C-terminus of the sequence (TETSQVAPA), this is not resolved in density and not modelled. Glvi linker sequence (SQPARAA), region substituted Ser322-Ala408,Includes an ...Details: Includes an 1D4 tag at C-terminus of the sequence (TETSQVAPA), this is not resolved in density and not modelled. Glvi linker sequence (SQPARAA), region substituted Ser322-Ala408,Includes an 1D4 tag at C-terminus of the sequence (TETSQVAPA), this is not resolved in density and not modelled. Glvi linker sequence (SQPARAA), region substituted Ser322-Ala408
Source: (gene. exp.) Homo sapiens (human) / Gene: GABRG2 / Production host: Homo sapiens (human) / References: UniProt: P18507

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Protein / Antibody / Non-polymers , 3 types, 6 molecules BEFG

#10: Chemical ChemComp-H0Z / bicuculline methochloride


Mass: 367.352 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C20H17NO6 / Feature type: SUBJECT OF INVESTIGATION
#2: Protein GABA-A receptor beta-3-bril


Mass: 51615.117 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: Modified glvi sequence containing the Escherichia coli soluble cytochrome B562RIL41 (BRIL, amino acids 23-130, ADLE...QKYL, Uniprot P0ABE7) to give the sequence SQPAGT-BRIL-TGRAA. Linker ...Details: Modified glvi sequence containing the Escherichia coli soluble cytochrome B562RIL41 (BRIL, amino acids 23-130, ADLE...QKYL, Uniprot P0ABE7) to give the sequence SQPAGT-BRIL-TGRAA. Linker regions substituted Gly308-Asn421. This BRIL domain was present in the protein but not resolved in the cryo-EM density and is therefore not included in the atomic model.
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#4: Antibody a3NB77


Mass: 13594.054 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Lama glama (llama) / Production host: Homo sapiens (human)

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Sugars , 5 types, 11 molecules

#5: Polysaccharide alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D- ...alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 1235.105 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-3[DManpa1-6]DManpa1-6[DManpa1-3]DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,7,6/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3-3-3-3/a4-b1_b4-c1_c3-d1_c6-e1_e3-f1_e6-g1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{}[(6+1)][a-D-Manp]{[(3+1)][a-D-Manp]{}[(6+1)][a-D-Manp]{}}}}}LINUCSPDB-CARE
#6: Polysaccharide 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 424.401 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/1,2,1/[a2122h-1b_1-5_2*NCC/3=O]/1-1/a4-b1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}}LINUCSPDB-CARE
#7: Polysaccharide alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D- ...alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 1072.964 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-3DManpa1-6[DManpa1-3]DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,6,5/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3-3-3/a4-b1_b4-c1_c3-d1_c6-e1_e3-f1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{}[(6+1)][a-D-Manp]{[(3+1)][a-D-Manp]{}}}}}LINUCSPDB-CARE
#8: Polysaccharide alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2- ...alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 910.823 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-3[DManpa1-6]DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,5,4/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3-3/a4-b1_b4-c1_c3-d1_c6-e1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{}[(6+1)][a-D-Manp]{}}}}LINUCSPDB-CARE
#9: Sugar
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Details

Has ligand of interestY
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

Component
IDNameTypeDetails (eV)Entity IDParent-IDSource
1alpha3-beta3-gamma2 GABA-A receptor in complex with bicuculline and nanobody a3NB77COMPLEXThe heteropentameric alpha3beta3gamma2 GABA(A) receptor was recombinantly expressed in HEK293 cells. Constructs were engineered with the M3- M4 intracellular domain replaced either by a GLVI linker sequence (SQPARAA) or by a modified GLVI sequence containing Escherichia coli cytochrome B562RIL41. The sample was prepared in the presence of bicuculline and nanobody a3NB77#1-#40RECOMBINANT
2GABA-A receptorCOMPLEX#1-#31RECOMBINANT
3Nanobody a3NB77COMPLEX#41RECOMBINANT
Source (natural)
IDEntity assembly-IDOrganismNcbi tax-ID
21Homo sapiens (human)9606
32Homo sapiens (human)9606
33Lama glama (llama)9844
Source (recombinant)
IDEntity assembly-IDOrganismNcbi tax-IDCellPlasmid
21Homo sapiens (human)9606
22Homo sapiens (human)9606Expi293F GnTI-pHLsec
33Homo sapiens (human)9606Expi293FpHLsec
Buffer solutionpH: 7.6
Details: 75 millimolar sodium chloride; 12.5 millimolar HEPES, pH 7.6; 1.5 millimolar 1D4 peptide
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Details: Nanodisc-reconstituted alpha3beta3gamma2 GABA-A receptor in complex with with bicuculline and nanobody a3NB77
Specimen supportGrid material: GOLD / Grid mesh size: 300 divisions/in. / Grid type: UltrAuFoil R1.2/1.3
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 95 % / Chamber temperature: 277 K

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: OTHER
Electron lensMode: BRIGHT FIELD / Nominal magnification: 130000 X / Nominal defocus max: 2200 nm / Nominal defocus min: 800 nm / Cs: 2.7 mm / C2 aperture diameter: 50 µm
Specimen holderCryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image recording
IDImaging-IDElectron dose (e/Å2)Film or detector modelNum. of real images
1151.25GATAN K3 BIOQUANTUM (6k x 4k)4031
2151.25GATAN K3 BIOQUANTUM (6k x 4k)
3151.25GATAN K3 BIOQUANTUM (6k x 4k)

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Processing

EM software
IDNameVersionCategoryImage processing-ID
1cryoSPARC4.4.1 to 4.7.1particle selection1
4cryoSPARC4.4.1 to 4.7.1CTF correction1
7UCSF Chimera1.8model fitting
9cryoSPARC4.4.1 to 4.7.1initial Euler assignment1
10cryoSPARC4.4.1 to 4.7.1final Euler assignment1
12cryoSPARC4.4.1 to 4.7.13D reconstruction1
13cryoSPARC4.4.1 to 4.7.1particle selection2
18cryoSPARC4.4.1 to 4.7.13D reconstruction2
19cryoSPARC4.4.1 to 4.7.1particle selection3
20cryoSPARCparticle selection3
25cryoSPARC3D reconstruction3
26cryoSPARCparticle selection4
31cryoSPARC3D reconstruction4
32PHENIX1.20.1_4487model refinement
Image processing
IDImage recording-ID
11
22
33
41
CTF correction
IDEM image processing-IDType
11PHASE FLIPPING AND AMPLITUDE CORRECTION
22PHASE FLIPPING AND AMPLITUDE CORRECTION
33PHASE FLIPPING AND AMPLITUDE CORRECTION
44PHASE FLIPPING AND AMPLITUDE CORRECTION
Particle selection
IDImage processing-IDNum. of particles selected
11630021
21630021
31630021
42630021
52
62
73
83
93
104
114
124
Symmetry
IDImage processing-IDEntry-IDPoint symmetry
119TNBC1 (asymmetric)
219TNBC1 (asymmetric)
319TNBC1 (asymmetric)
419TNBC1 (asymmetric)
519TNBC1 (asymmetric)
619TNBC1 (asymmetric)
719TNBC1 (asymmetric)
819TNBC1 (asymmetric)
919TNBC1 (asymmetric)
1019TNBC1 (asymmetric)
1119TNBC1 (asymmetric)
1219TNBC1 (asymmetric)
1319TNBC1 (asymmetric)
1419TNBC1 (asymmetric)
1519TNBC1 (asymmetric)
1619TNBC1 (asymmetric)
1729TNBC1 (asymmetric)
1829TNB
1929TNBC1 (asymmetric)
2029TNB
2129TNBC1 (asymmetric)
2229TNB
2329TNBC1 (asymmetric)
2429TNB
2529TNBC1 (asymmetric)
2629TNB
2729TNBC1 (asymmetric)
2829TNB
2929TNBC1 (asymmetric)
3029TNB
3129TNBC1 (asymmetric)
3229TNB
3339TNB
3439TNB
3539TNB
3639TNB
3739TNB
3839TNB
3939TNB
4039TNB
4139TNB
4239TNB
4339TNB
4439TNB
4539TNB
4639TNB
4739TNB
4839TNB
4949TNB
5049TNB
5149TNB
5249TNB
5349TNB
5449TNB
5549TNB
5649TNB
5749TNB
5849TNB
5949TNB
6049TNB
6149TNB
6249TNB
6349TNB
6449TNB
3D reconstruction
IDResolution (Å)Resolution methodNum. of particlesImage processing-IDEntry-IDSymmetry type
12.8FSC 0.143 CUT-OFF15256219TNBPOINT
22.87FSC 0.143 CUT-OFF15256219TNBPOINT
32.8FSC 0.143 CUT-OFF15256219TNBPOINT
42.87FSC 0.143 CUT-OFF15256219TNBPOINT
52.8FSC 0.143 CUT-OFF15256219TNBPOINT
62.87FSC 0.143 CUT-OFF15256219TNBPOINT
72.8FSC 0.143 CUT-OFF15256219TNBPOINT
82.87FSC 0.143 CUT-OFF15256219TNBPOINT
92.8FSC 0.143 CUT-OFF15256229TNBPOINT
102.87FSC 0.143 CUT-OFF15256229TNBPOINT
112.8FSC 0.143 CUT-OFF15256229TNBPOINT
122.87FSC 0.143 CUT-OFF15256229TNBPOINT
132.8FSC 0.143 CUT-OFF15256229TNBPOINT
142.87FSC 0.143 CUT-OFF15256229TNBPOINT
152.8FSC 0.143 CUT-OFF15256229TNBPOINT
162.87FSC 0.143 CUT-OFF15256229TNBPOINT
172.8FSC 0.143 CUT-OFF15256239TNBPOINT
182.87FSC 0.143 CUT-OFF15256239TNBPOINT
192.8FSC 0.143 CUT-OFF15256239TNBPOINT
202.87FSC 0.143 CUT-OFF15256239TNBPOINT
212.8FSC 0.143 CUT-OFF15256239TNBPOINT
222.87FSC 0.143 CUT-OFF15256239TNBPOINT
232.8FSC 0.143 CUT-OFF15256239TNBPOINT
242.87FSC 0.143 CUT-OFF15256239TNBPOINT
252.8FSC 0.143 CUT-OFF15256249TNBPOINT
262.87FSC 0.143 CUT-OFF15256249TNBPOINT
272.8FSC 0.143 CUT-OFF15256249TNBPOINT
282.87FSC 0.143 CUT-OFF15256249TNBPOINT
292.8FSC 0.143 CUT-OFF15256249TNBPOINT
302.87FSC 0.143 CUT-OFF15256249TNBPOINT
312.8FSC 0.143 CUT-OFF15256249TNBPOINT
322.87FSC 0.143 CUT-OFF15256249TNBPOINT
RefinementHighest resolution: 2.8 Å
Stereochemistry target values: REAL-SPACE (WEIGHTED MAP SUM AT ATOM CENTERS)
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.00316163
ELECTRON MICROSCOPYf_angle_d0.52922005
ELECTRON MICROSCOPYf_dihedral_angle_d5.0712416
ELECTRON MICROSCOPYf_chiral_restr0.0682602
ELECTRON MICROSCOPYf_plane_restr0.0042686

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