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Yorodumi- PDB-9r9p: Yeast 80S with nascent chain in complex with Ssb1-ADP in the S2 state -
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Open data
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Basic information
| Entry | Database: PDB / ID: 9r9p | ||||||||||||||||||||||||||||||
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| Title | Yeast 80S with nascent chain in complex with Ssb1-ADP in the S2 state | ||||||||||||||||||||||||||||||
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Keywords | RIBOSOME / Hsp70 / nascent chain / 80S / Ssb | ||||||||||||||||||||||||||||||
| Function / homology | Function and homology informationMitochondrial unfolded protein response (UPRmt) / translational frameshifting / Gluconeogenesis / Glycolysis / 'de novo' cotranslational protein folding / phosphoglycerate kinase / phosphoglycerate kinase activity / HSF1-dependent transactivation / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / Regulation of HSF1-mediated heat shock response ...Mitochondrial unfolded protein response (UPRmt) / translational frameshifting / Gluconeogenesis / Glycolysis / 'de novo' cotranslational protein folding / phosphoglycerate kinase / phosphoglycerate kinase activity / HSF1-dependent transactivation / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / Regulation of HSF1-mediated heat shock response / regulation of amino acid metabolic process / negative regulation of glucose mediated signaling pathway / positive regulation of translational fidelity / : / RMTs methylate histone arginines / Protein methylation / mTORC1-mediated signalling / Protein hydroxylation / ribosome-associated ubiquitin-dependent protein catabolic process / pre-mRNA 5'-splice site binding / GDP-dissociation inhibitor activity / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / nonfunctional rRNA decay / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / response to cycloheximide / Ribosomal scanning and start codon recognition / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / preribosome, small subunit precursor / Major pathway of rRNA processing in the nucleolus and cytosol / mRNA destabilization / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / non-chaperonin molecular chaperone ATPase / negative regulation of mRNA splicing, via spliceosome / : / positive regulation of protein kinase activity / preribosome, large subunit precursor / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of translational frameshifting / L13a-mediated translational silencing of Ceruloplasmin expression / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translational elongation / G-protein alpha-subunit binding / 90S preribosome / Ub-specific processing proteases / translational termination / regulation of translational fidelity / ribosomal subunit export from nucleus / protein-RNA complex assembly / maturation of LSU-rRNA / heat shock protein binding / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translation regulator activity / ribosomal small subunit export from nucleus / DNA-(apurinic or apyrimidinic site) endonuclease activity / Neutrophil degranulation / protein folding chaperone / rescue of stalled cytosolic ribosome / cellular response to amino acid starvation / protein kinase C binding / ribosomal large subunit biogenesis / ribosome assembly / glycolytic process / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / gluconeogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / macroautophagy / maturation of SSU-rRNA / ATP-dependent protein folding chaperone / small-subunit processome / translational initiation / ADP binding / maintenance of translational fidelity / cytoplasmic stress granule / rRNA processing / unfolded protein binding / ribosome biogenesis / ribosome binding / protein refolding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / 5S rRNA binding / ribosomal large subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / cytoplasmic translation / calmodulin binding / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / G protein-coupled receptor signaling pathway / negative regulation of gene expression Similarity search - Function | ||||||||||||||||||||||||||||||
| Biological species | ![]() | ||||||||||||||||||||||||||||||
| Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3 Å | ||||||||||||||||||||||||||||||
Authors | Grundmann, L. / Zhang, Y. / Grishkovskaya, I. / Rospert, S. / Haselbach, D. | ||||||||||||||||||||||||||||||
| Funding support | Germany, 5items
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Citation | Journal: To Be PublishedTitle: The cotranslational cycle of the ribosome-bound Hsp70 homolog Ssb Authors: Zhang, Y. / Grundmann, L. / Vollmar, L. / Schimpf, J. / Huebscher, V. / Grishkovskaya, I. / Moddemann, A. / Werner, K. / Hugel, T. / Haselbach, D. / Rospert, S. | ||||||||||||||||||||||||||||||
| History |
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Structure visualization
| Structure viewer | Molecule: Molmil Jmol/JSmol |
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Downloads & links
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Download
| PDBx/mmCIF format | 9r9p.cif.gz | 9.7 MB | Display | PDBx/mmCIF format |
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| PDB format | pdb9r9p.ent.gz | Display | PDB format | |
| PDBx/mmJSON format | 9r9p.json.gz | Tree view | PDBx/mmJSON format | |
| Others | Other downloads |
-Validation report
| Summary document | 9r9p_validation.pdf.gz | 2.2 MB | Display | wwPDB validaton report |
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| Full document | 9r9p_full_validation.pdf.gz | 2.3 MB | Display | |
| Data in XML | 9r9p_validation.xml.gz | 326.7 KB | Display | |
| Data in CIF | 9r9p_validation.cif.gz | 570.9 KB | Display | |
| Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/r9/9r9p ftp://data.pdbj.org/pub/pdb/validation_reports/r9/9r9p | HTTPS FTP |
-Related structure data
| Related structure data | ![]() 53861MC ![]() 9r9oC M: map data used to model this data C: citing same article ( |
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| Similar structure data | Similarity search - Function & homology F&H Search |
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Links
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Assembly
| Deposited unit | ![]()
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Components
-RNA chain , 6 types, 6 molecules 56C1C2C3C4
| #1: RNA chain | Mass: 75091.320 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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| #2: RNA chain | Mass: 24445.562 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
| #5: RNA chain | Mass: 1097493.875 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
| #6: RNA chain | Mass: 579761.938 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
| #7: RNA chain | Mass: 50682.922 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
| #8: RNA chain | Mass: 38951.105 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
-Protein , 3 types, 3 molecules ABSg
| #3: Protein | Mass: 66682.406 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: SSB1, YG101, YDL229W / Production host: ![]() References: UniProt: P11484, non-chaperonin molecular chaperone ATPase |
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| #4: Protein | Mass: 9061.538 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: Flag-Pgk1_1-70 / Source: (synth.) ![]() |
| #79: Protein | Mass: 34841.219 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
+60S ribosomal protein ... , 38 types, 38 molecules LALBLCLDLELFLGLHLILJLLLMLNLOLPLQLRLSLTLULVLXLYLZLaLbLcLdLeLf...
-Large ribosomal subunit protein ... , 2 types, 2 molecules LWLn
| #30: Protein | Mass: 17595.660 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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| #46: Protein/peptide | Mass: 3354.243 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
+40S ribosomal protein ... , 25 types, 25 molecules SASBSCSESFSGSHSISJSKSLSNSOSPSQSRSSSTSVSWSXSYSZSbSe
-Small ribosomal subunit protein ... , 5 types, 5 molecules SDSUSaScSd
| #52: Protein | Mass: 26542.789 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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| #68: Protein | Mass: 13929.044 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
| #74: Protein | Mass: 13538.922 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
| #76: Protein | Mass: 7578.822 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
| #77: Protein | Mass: 6675.723 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
-Details
| Has protein modification | Y |
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-Experimental details
-Experiment
| Experiment | Method: ELECTRON MICROSCOPY |
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| EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
| Component | Name: S. cerevisiae 80S ribosome in a non-rotated, post-translocation state with a P-site tRNA-Met-elongation, a nacent chain (Pgk 1-70), and Ssb1 in the ADP state adapting the S2 conformation Type: RIBOSOME / Entity ID: all / Source: NATURAL |
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| Molecular weight | Experimental value: NO |
| Source (natural) | Organism: ![]() |
| Source (recombinant) | Organism: ![]() |
| Buffer solution | pH: 7.4 |
| Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
| Specimen support | Grid material: COPPER / Grid mesh size: 200 divisions/in. / Grid type: Quantifoil R3.5/1 |
| Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
| Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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| Microscopy | Model: TFS KRIOS |
| Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
| Electron lens | Mode: BRIGHT FIELD / Nominal magnification: 130000 X / Nominal defocus max: 2000 nm / Nominal defocus min: 1000 nm / Alignment procedure: COMA FREE |
| Specimen holder | Cryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER |
| Image recording | Electron dose: 40 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k) / Num. of grids imaged: 3 / Num. of real images: 54286 |
| EM imaging optics | Energyfilter name: TFS Selectris X / Energyfilter slit width: 10 eV |
| Image scans | Width: 4096 / Height: 4096 |
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Processing
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| CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
| Particle selection | Num. of particles selected: 3512138 | ||||||||||||||||||||||||
| Symmetry | Point symmetry: C1 (asymmetric) | ||||||||||||||||||||||||
| 3D reconstruction | Resolution: 3 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 14049 / Algorithm: FOURIER SPACE / Symmetry type: POINT | ||||||||||||||||||||||||
| Refinement | Cross valid method: NONE Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2 | ||||||||||||||||||||||||
| Displacement parameters | Biso mean: 70.69 Å2 | ||||||||||||||||||||||||
| Refine LS restraints |
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