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データを開く
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基本情報
| 登録情報 | データベース: PDB / ID: 9qqb | ||||||||||||
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| タイトル | Quaternary complex of a translating ribosome, NAC, NMT1, and NatA | ||||||||||||
要素 |
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キーワード | RIBOSOME / Translation / co-translational protein processing | ||||||||||||
| 機能・相同性 | 機能・相同性情報negative regulation of maintenance of mitotic sister chromatid cohesion, centromeric / positive regulation of protein refolding / [histone H4]-N-methyl-L-lysine20 N-methyltransferase / protein-N-terminal-glutamate acetyltransferase activity / N-terminal amino-acid Nalpha-acetyltransferase NatA / negative regulation of striated muscle cell apoptotic process / [histone H4]-lysine20 N-methyltransferase / histone H4K20 monomethyltransferase activity / regulation of skeletal muscle fiber development / NatA complex ...negative regulation of maintenance of mitotic sister chromatid cohesion, centromeric / positive regulation of protein refolding / [histone H4]-N-methyl-L-lysine20 N-methyltransferase / protein-N-terminal-glutamate acetyltransferase activity / N-terminal amino-acid Nalpha-acetyltransferase NatA / negative regulation of striated muscle cell apoptotic process / [histone H4]-lysine20 N-methyltransferase / histone H4K20 monomethyltransferase activity / regulation of skeletal muscle fiber development / NatA complex / myristoyltransferase activity / protein N-terminal-serine acetyltransferase activity / N-terminal peptidyl-glycine N-myristoylation / peptidyl-lysine N6-myristoyltransferase activity / positive regulation of cell proliferation involved in heart morphogenesis / protein-N-terminal-alanine acetyltransferase activity / Late Phase of HIV Life Cycle / histone H4K20me methyltransferase activity / positive regulation of skeletal muscle tissue growth / ketone metabolic process / protein-N-terminal amino-acid acetyltransferase activity / regulation of opsin-mediated signaling pathway / negative regulation of protein localization to endoplasmic reticulum / nascent polypeptide-associated complex / cardiac ventricle development / Activation, myristolyation of BID and translocation to mitochondria / positive regulation of protein localization to mitochondrion / glycylpeptide N-tetradecanoyltransferase / glycylpeptide N-tetradecanoyltransferase activity / acetyltransferase activator activity / N-acetyltransferase activity / heart trabecula morphogenesis / skeletal muscle tissue regeneration / S-adenosyl-L-methionine binding / protein localization to membrane / positive regulation of double-strand break repair via nonhomologous end joining / laminin receptor activity / Peptide chain elongation / Selenocysteine synthesis / Formation of a pool of free 40S subunits / Eukaryotic Translation Termination / SRP-dependent cotranslational protein targeting to membrane / Response of EIF2AK4 (GCN2) to amino acid deficiency / Viral mRNA Translation / 90S preribosome / ubiquitin ligase inhibitor activity / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / positive regulation of signal transduction by p53 class mediator / GTP hydrolysis and joining of the 60S ribosomal subunit / L13a-mediated translational silencing of Ceruloplasmin expression / pericentric heterochromatin / Major pathway of rRNA processing in the nucleolus and cytosol / phagocytic cup / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / protein-RNA complex assembly / maturation of LSU-rRNA / eNOS activation / translation regulator activity / rough endoplasmic reticulum / ribosomal small subunit export from nucleus / laminin binding / gastrulation / 転移酵素; アシル基を移すもの; アミノアシル基以外のアシル基を移すもの / MDM2/MDM4 family protein binding / cytosolic ribosome / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / ribosomal large subunit biogenesis / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / positive regulation of apoptotic signaling pathway / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / wound healing / small-subunit processome / protein maturation / spindle / Regulation of expression of SLITs and ROBOs / cytoplasmic ribonucleoprotein granule / rRNA processing / positive regulation of canonical Wnt signaling pathway / rhythmic process / regulation of translation / antimicrobial humoral immune response mediated by antimicrobial peptide / heparin binding / protein transport / Inactivation, recovery and regulation of the phototransduction cascade / large ribosomal subunit / ribosomal small subunit assembly / virus receptor activity / ribosome binding / ribosomal small subunit biogenesis / 5S rRNA binding / small ribosomal subunit / ribosomal large subunit assembly / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / angiogenesis / large ribosomal subunit rRNA binding / methylation / transcription regulator complex 類似検索 - 分子機能 | ||||||||||||
| 生物種 | Homo sapiens (ヒト)![]() | ||||||||||||
| 手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.43 Å | ||||||||||||
データ登録者 | Echeverria, B. / Jaskolowski, M. / Scaiola, A. / Ban, N. | ||||||||||||
| 資金援助 | スイス, European Union, 3件
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引用 | ジャーナル: Mol Cell / 年: 2025タイトル: Mechanism of cotranslational protein N-myristoylation in human cells. 著者: Martin Gamerdinger / Blanca Echeverria / Alfred M Lentzsch / Nicolas Burg / Ziyi Fan / Mateusz Jaskolowski / Alain Scaiola / Selina Piening / Shu-Ou Shan / Nenad Ban / Elke Deuerling / ![]() 要旨: N-myristoyltransferases (NMTs) cotranslationally transfer the fatty acid myristic acid to the N terminus of newly synthesized proteins, regulating their function and cellular localization. These ...N-myristoyltransferases (NMTs) cotranslationally transfer the fatty acid myristic acid to the N terminus of newly synthesized proteins, regulating their function and cellular localization. These enzymes are important drug targets for the treatment of cancer and viral infections. N-myristoylation of nascent proteins occurs specifically on N-terminal glycine residues after the excision of the initiator methionine by methionine aminopeptidases (METAPs). How NMTs interact with ribosomes and gain timely and specific access to their substrates remains unknown. Here, we show that human NMT1 exchanges with METAP1 at the ribosomal tunnel exit to form an active cotranslational complex together with the nascent polypeptide-associated complex (NAC). NMT1 binding is sequence selective and specifically triggered by methionine excision, which exposes the N-myristoylation motif in the nascent chain. The revealed mode of interaction of NMT1 with NAC and the methionine-cleaved nascent protein elucidates how a specific subset of proteins can be efficiently N-myristoylated in human cells. | ||||||||||||
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構造の表示
| 構造ビューア | 分子: Molmil Jmol/JSmol |
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ダウンロードとリンク
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ダウンロード
| PDBx/mmCIF形式 | 9qqb.cif.gz | 6.6 MB | 表示 | PDBx/mmCIF形式 |
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| PDB形式 | pdb9qqb.ent.gz | 表示 | PDB形式 | |
| PDBx/mmJSON形式 | 9qqb.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
| その他 | その他のダウンロード |
-検証レポート
| アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/qq/9qqb ftp://data.pdbj.org/pub/pdb/validation_reports/qq/9qqb | HTTPS FTP |
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-関連構造データ
| 関連構造データ | ![]() 53296MC ![]() 9qqaC M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 ( |
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| 類似構造データ | 類似検索 - 機能・相同性 F&H 検索 |
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リンク
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集合体
| 登録構造単位 | ![]()
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| 1 |
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要素
+RNA鎖 , 5種, 5分子 B5B7B8A2AT
+Large ribosomal subunit protein ... , 10種, 10分子 BABCBDBLBOBPBSBXBoBs
+Ribosomal protein ... , 10種, 10分子 BBBFBNBRBVBWBYBeBjBv
+60S ribosomal protein ... , 21種, 21分子 BEBGBHBIBJBMBTBUBZBaBbBcBdBfBgBhBiBkBlBpBt
+タンパク質・ペプチド , 1種, 1分子 BK
+タンパク質 , 8種, 8分子 BQBrNtNuMAADAEAj
+Ubiquitin-ribosomal protein ... , 2種, 2分子 BmAC
+N-alpha-acetyltransferase ... , 2種, 2分子 XAXB
+Small ribosomal subunit protein ... , 20種, 20分子 AAABAFAGAZAeAiAkAlAmAnAoApAqArAtAuAvAyAz
+40S ribosomal protein ... , 10種, 10分子 AaAbAcAdAfAgAhAsAwAx
+非ポリマー , 7種, 2696分子 












+詳細
-実験情報
-実験
| 実験 | 手法: 電子顕微鏡法 |
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| EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
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試料調製
| 構成要素 | 名称: Quaternary complex of translating ribosome, NAC, NMT1 and NatA タイプ: RIBOSOME / Entity ID: #1-#13, #15-#89 / 由来: NATURAL |
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| 由来(天然) | 生物種: ![]() |
| 緩衝液 | pH: 7.6 |
| 試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
| 急速凍結 | 装置: FEI VITROBOT MARK IV / 凍結剤: ETHANE-PROPANE |
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電子顕微鏡撮影
| 実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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| 顕微鏡 | モデル: TFS KRIOS |
| 電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: FLOOD BEAM |
| 電子レンズ | モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2700 nm / 最小 デフォーカス(公称値): 700 nm |
| 撮影 | 電子線照射量: 60 e/Å2 / フィルム・検出器のモデル: GATAN K3 (6k x 4k) |
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解析
| EMソフトウェア | 名称: PHENIX / バージョン: 1.21.2_5419 / カテゴリ: モデル精密化 |
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| CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION |
| 3次元再構成 | 解像度: 3.43 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 15709 / 対称性のタイプ: POINT |
| 精密化 | 交差検証法: NONE |
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万見について




Homo sapiens (ヒト)

スイス, European Union, 3件
引用




PDBj




















































FIELD EMISSION GUN