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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 9qqa | ||||||||||||
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タイトル | Ternary complex of translating ribosome, NAC and NMT1 | ||||||||||||
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![]() | RIBOSOME / Translation / co-translational protein processing | ||||||||||||
機能・相同性 | ![]() negative regulation of protein localization to endoplasmic reticulum / nascent polypeptide-associated complex / negative regulation of striated muscle cell apoptotic process / [histone H4]-N-methyl-L-lysine20 N-methyltransferase / regulation of skeletal muscle fiber development / positive regulation of cell proliferation involved in heart morphogenesis / myristoyltransferase activity / N-terminal peptidyl-glycine N-myristoylation / peptidyl-lysine N6-myristoyltransferase activity / [histone H4]-lysine20 N-methyltransferase ...negative regulation of protein localization to endoplasmic reticulum / nascent polypeptide-associated complex / negative regulation of striated muscle cell apoptotic process / [histone H4]-N-methyl-L-lysine20 N-methyltransferase / regulation of skeletal muscle fiber development / positive regulation of cell proliferation involved in heart morphogenesis / myristoyltransferase activity / N-terminal peptidyl-glycine N-myristoylation / peptidyl-lysine N6-myristoyltransferase activity / [histone H4]-lysine20 N-methyltransferase / Late Phase of HIV Life Cycle / positive regulation of skeletal muscle tissue growth / histone H4K20 methyltransferase activity / ketone metabolic process / regulation of opsin-mediated signaling pathway / cardiac ventricle development / Activation, myristolyation of BID and translocation to mitochondria / positive regulation of establishment of protein localization to mitochondrion / glycylpeptide N-tetradecanoyltransferase / glycylpeptide N-tetradecanoyltransferase activity / heart trabecula morphogenesis / skeletal muscle tissue regeneration / S-adenosyl-L-methionine binding / protein localization to membrane / ribosomal subunit / exit from mitosis / optic nerve development / laminin receptor activity / retinal ganglion cell axon guidance / positive regulation of double-strand break repair via nonhomologous end joining / Peptide chain elongation / Selenocysteine synthesis / Formation of a pool of free 40S subunits / ubiquitin ligase inhibitor activity / Eukaryotic Translation Termination / positive regulation of signal transduction by p53 class mediator / Response of EIF2AK4 (GCN2) to amino acid deficiency / SRP-dependent cotranslational protein targeting to membrane / Viral mRNA Translation / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / 90S preribosome / GTP hydrolysis and joining of the 60S ribosomal subunit / L13a-mediated translational silencing of Ceruloplasmin expression / phagocytic cup / Major pathway of rRNA processing in the nucleolus and cytosol / protein-RNA complex assembly / pericentric heterochromatin / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / maturation of LSU-rRNA / ribosomal small subunit export from nucleus / laminin binding / rough endoplasmic reticulum / eNOS activation / translation regulator activity / gastrulation / cytosolic ribosome / MDM2/MDM4 family protein binding / 転移酵素; アシル基を移すもの; アミノアシル基以外のアシル基を移すもの / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / cellular response to interleukin-4 / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / positive regulation of apoptotic signaling pathway / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / wound healing / spindle / Regulation of expression of SLITs and ROBOs / cytoplasmic ribonucleoprotein granule / antimicrobial humoral immune response mediated by antimicrobial peptide / rRNA processing / rhythmic process / positive regulation of canonical Wnt signaling pathway / Inactivation, recovery and regulation of the phototransduction cascade / protein transport / large ribosomal subunit / regulation of translation / heparin binding / ribosome binding / virus receptor activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / retina development in camera-type eye / small ribosomal subunit / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / small ribosomal subunit rRNA binding / defense response to Gram-negative bacterium / methylation / killing of cells of another organism / cytosolic large ribosomal subunit / in utero embryonic development / perikaryon / cytoplasmic translation / cell differentiation / transcription coactivator activity / tRNA binding 類似検索 - 分子機能 | ||||||||||||
生物種 | ![]() ![]() ![]() | ||||||||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.8 Å | ||||||||||||
![]() | Echeverria, B. / Jaskolowski, M. / Scaiola, A. / Ban, N. | ||||||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Mechanism of cotranslational protein N-myristoylation in human cells 著者: Gamerdinger, M. / Echeverria, B. / Lentzsch, A.M. / Burg, N. / Fan, Z. / Jaskolowski, M. / Scaiola, A. / Piening, S. / Shan, S.O. / Ban, N. / Deuerling, E. | ||||||||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 6.2 MB | 表示 | ![]() |
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PDB形式 | ![]() | 表示 | ![]() | |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 2.3 MB | 表示 | ![]() |
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文書・詳細版 | ![]() | 2.6 MB | 表示 | |
XML形式データ | ![]() | 411.9 KB | 表示 | |
CIF形式データ | ![]() | 690 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 53295MC ![]() 9qqbC M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
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集合体
登録構造単位 | ![]()
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要素
+RNA鎖 , 5種, 5分子 A2ATB5B7B8
+Small ribosomal subunit protein ... , 20種, 20分子 AAABAFAGAZAeAiAkAlAmAnAoApAqArAtAuAvAyAz
+Ubiquitin-ribosomal protein ... , 2種, 2分子 ACBm
+タンパク質 , 8種, 8分子 ADAEAjBQBrMANtNu
+40S ribosomal protein ... , 10種, 10分子 AaAbAcAdAfAgAhAsAwAx
+Large ribosomal subunit protein ... , 9種, 9分子 BABCBLBOBPBSBXBoBs
+Ribosomal protein ... , 10種, 10分子 BBBFBNBRBVBWBYBeBjBv
+60S ribosomal protein ... , 21種, 21分子 BEBGBHBIBJBMBTBUBZBaBbBcBdBfBgBhBiBkBlBpBt
+タンパク質・ペプチド , 1種, 1分子 BK
+非ポリマー , 6種, 2663分子 










+詳細
-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
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試料調製
構成要素 | 名称: Ternary complex of translating ribosome, NAC and NMT1 タイプ: RIBOSOME / Entity ID: #1-#86 / 由来: NATURAL |
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由来(天然) | 生物種: ![]() ![]() |
緩衝液 | pH: 7.6 |
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
急速凍結 | 装置: FEI VITROBOT MARK IV / 凍結剤: ETHANE-PROPANE |
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電子顕微鏡撮影
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: TFS KRIOS |
電子銃 | 電子線源: ![]() |
電子レンズ | モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2700 nm / 最小 デフォーカス(公称値): 700 nm |
撮影 | 電子線照射量: 60 e/Å2 / フィルム・検出器のモデル: GATAN K3 (6k x 4k) |
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解析
EMソフトウェア | 名称: PHENIX / カテゴリ: モデル精密化 |
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CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION |
3次元再構成 | 解像度: 2.8 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 87143 / 対称性のタイプ: POINT |
精密化 | 交差検証法: NONE |