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- PDB-9n5g: RNA polymerase II elongation complex with 8-oxoG at +1 site, ATP ... -

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基本情報

登録情報
データベース: PDB / ID: 9n5g
タイトルRNA polymerase II elongation complex with 8-oxoG at +1 site, ATP in both A- and E-site
要素
  • (DNA-directed RNA polymerase II subunit ...) x 5
  • (DNA-directed RNA polymerases I, II, and III subunit ...) x 5
  • Non-template strand DNA
  • RNA
  • Template strand DNA
キーワードTRANSCRIPTION / RNA polymerase II / oxidative damage / in vitro transcription / 8-oxoguanine
機能・相同性
機能・相同性情報


RNA Polymerase I Transcription Initiation / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / mRNA Capping / RNA polymerase II transcribes snRNA genes / termination of RNA polymerase II transcription / TP53 Regulates Transcription of DNA Repair Genes / RNA Polymerase II Promoter Escape ...RNA Polymerase I Transcription Initiation / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / mRNA Capping / RNA polymerase II transcribes snRNA genes / termination of RNA polymerase II transcription / TP53 Regulates Transcription of DNA Repair Genes / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / termination of RNA polymerase III transcription / RNA Polymerase II Pre-transcription Events / RNA-templated transcription / Formation of TC-NER Pre-Incision Complex / transcription initiation at RNA polymerase III promoter / RNA Polymerase I Promoter Escape / termination of RNA polymerase I transcription / nucleolar large rRNA transcription by RNA polymerase I / Gap-filling DNA repair synthesis and ligation in TC-NER / transcription initiation at RNA polymerase I promoter / Estrogen-dependent gene expression / maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II / transcription by RNA polymerase III / Dual incision in TC-NER / translesion synthesis / RNA polymerase I complex / transcription elongation by RNA polymerase I / RNA polymerase III complex / RNA polymerase II, core complex / tRNA transcription by RNA polymerase III / transcription by RNA polymerase I / transcription-coupled nucleotide-excision repair / transcription initiation at RNA polymerase II promoter / transcription elongation by RNA polymerase II / ribonucleoside binding / DNA-directed RNA polymerase / cytoplasmic stress granule / DNA-directed RNA polymerase activity / peroxisome / ribosome biogenesis / nucleic acid binding / transcription by RNA polymerase II / protein dimerization activity / mRNA binding / nucleolus / mitochondrion / DNA binding / zinc ion binding / nucleoplasm / metal ion binding / nucleus / cytoplasm
類似検索 - 分子機能
RNA polymerase Rpb1 C-terminal repeat / RNA polymerase II, heptapeptide repeat, eukaryotic / Eukaryotic RNA polymerase II heptapeptide repeat. / RNA polymerase Rpb1, domain 6 / RNA polymerase Rpb1, domain 6 / RNA polymerase Rpb1, domain 7 / RNA polymerase Rpb1, domain 7 superfamily / RNA polymerase Rpb1, domain 7 / Pol II subunit B9, C-terminal zinc ribbon / RNA polymerase RBP11 ...RNA polymerase Rpb1 C-terminal repeat / RNA polymerase II, heptapeptide repeat, eukaryotic / Eukaryotic RNA polymerase II heptapeptide repeat. / RNA polymerase Rpb1, domain 6 / RNA polymerase Rpb1, domain 6 / RNA polymerase Rpb1, domain 7 / RNA polymerase Rpb1, domain 7 superfamily / RNA polymerase Rpb1, domain 7 / Pol II subunit B9, C-terminal zinc ribbon / RNA polymerase RBP11 / Zinc finger TFIIS-type signature. / RNA polymerase Rpb2, domain 5 / RNA polymerase Rpb2, domain 5 / RNA polymerase Rpb2, domain 4 / RNA polymerase Rpb2, domain 4 / DNA-directed RNA polymerase, M/15kDa subunit / RNA polymerases M/15 Kd subunit / RNA polymerase subunit 9 / DNA-directed RNA polymerase M, 15kDa subunit, conserved site / RNA polymerases M / 15 Kd subunits signature. / DNA-directed RNA polymerase subunit/transcription factor S / : / RNA polymerase, Rpb8 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / RNA polymerase Rpb8 / RNA polymerase subunit 8 / RNA polymerase, Rpb5, N-terminal / RNA polymerase Rpb5, N-terminal domain superfamily / RNA polymerase Rpb5, N-terminal domain / DNA-directed RNA polymerase, subunit RPB6 / DNA-directed RNA polymerase subunit RPABC5/Rpb10 / RNA polymerases, subunit N, zinc binding site / RNA polymerase subunit RPB10 / RNA polymerases N / 8 kDa subunit / RNA polymerases N / 8 Kd subunits signature. / DNA directed RNA polymerase, 7 kDa subunit / RNA polymerase archaeal subunit P/eukaryotic subunit RPABC4 / RNA polymerase, subunit H/Rpb5, conserved site / RNA polymerases H / 23 Kd subunits signature. / RNA polymerase subunit CX / DNA-directed RNA polymerase, 30-40kDa subunit, conserved site / DNA-directed RNA polymerase subunit Rpo3/Rpb3/RPAC1 / RNA polymerases D / 30 to 40 Kd subunits signature. / DNA-directed RNA polymerase Rpb11, 13-16kDa subunit, conserved site / DNA-directed RNA polymerase subunit Rpo11 / RNA polymerases L / 13 to 16 Kd subunits signature. / RNA polymerase subunit RPABC4/transcription elongation factor Spt4 / Zinc finger, TFIIS-type / Transcription factor S-II (TFIIS) / Zinc finger TFIIS-type profile. / C2C2 Zinc finger / DNA-directed RNA polymerase, RBP11-like dimerisation domain / RNA polymerase Rpb3/Rpb11 dimerisation domain / RNA polymerase, subunit H/Rpb5 C-terminal / DNA-directed RNA polymerase subunit Rpo5/Rpb5 / RPB5-like RNA polymerase subunit superfamily / RNA polymerase Rpb5, C-terminal domain / Archaeal Rpo6/eukaryotic RPB6 RNA polymerase subunit / DNA-directed RNA polymerase, 14-18kDa subunit, conserved site / RNA polymerases K / 14 to 18 Kd subunits signature. / DNA-directed RNA polymerase, subunit beta-prime / RNA polymerase Rpb6 / RNA polymerase, subunit omega/Rpo6/RPB6 / RNA polymerase Rpb6 / RNA polymerase Rpb2, domain 2 superfamily / RNA polymerase Rpb1, domain 3 superfamily / RPB6/omega subunit-like superfamily / DNA-directed RNA polymerase, insert domain / DNA-directed RNA polymerase, RpoA/D/Rpb3-type / RNA polymerase Rpb3/RpoA insert domain / RNA polymerase Rpb3/Rpb11 dimerisation domain / RNA polymerases D / RNA polymerase Rpb1, clamp domain superfamily / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 1 / RNA polymerase, beta subunit, protrusion / RNA polymerase beta subunit / RNA polymerase Rpb1, domain 1 / RNA polymerase, alpha subunit / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 4 / DNA-directed RNA polymerase, insert domain superfamily / RNA polymerase Rpb1, domain 2 / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 4 / RNA polymerase Rpb2, domain 2 / RNA polymerase Rpb2, domain 2 / RNA polymerase, RBP11-like subunit / RNA polymerase, N-terminal / RNA polymerase Rpb1, funnel domain superfamily / RNA polymerase I subunit A N-terminus / RNA polymerase, beta subunit, conserved site / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerase Rpb2, OB-fold / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerases beta chain signature. / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain
類似検索 - ドメイン・相同性
ADENOSINE-5'-TRIPHOSPHATE / DNA / DNA (> 10) / RNA / DNA-directed RNA polymerase II subunit RPB1 / DNA-directed RNA polymerase II subunit RPB2 / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 ...ADENOSINE-5'-TRIPHOSPHATE / DNA / DNA (> 10) / RNA / DNA-directed RNA polymerase II subunit RPB1 / DNA-directed RNA polymerase II subunit RPB2 / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / DNA-directed RNA polymerase II subunit RPB9 / DNA-directed RNA polymerase II subunit RPB11 / DNA-directed RNA polymerases I, II, and III subunit RPABC4
類似検索 - 構成要素
生物種Saccharomyces cerevisiae S288C (パン酵母)
synthetic construct (人工物)
手法X線回折 / シンクロトロン / 分子置換 / 解像度: 3.15 Å
データ登録者Oh, J. / Wang, D.
資金援助 米国, 韓国, 3件
組織認可番号国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01 GM102362 米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01 GM147652 米国
National Research Foundation (NRF, Korea)RS-2024-00344054 韓国
引用ジャーナル: J.Am.Chem.Soc. / 年: 2025
タイトル: Transcription-Coupled Template Reconfiguration of 8-Oxoguanine for Error-Prone Transcription Revealed by Time-Resolved X-ray Crystallography and Molecular Dynamics.
著者: Oh, J. / Konovalov, K.A. / Hou, P. / Chong, J. / Huang, X. / Wang, D.
履歴
登録2025年2月4日登録サイト: RCSB / 処理サイト: RCSB
改定 1.02025年8月13日Provider: repository / タイプ: Initial release

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構造の表示

構造ビューア分子:
MolmilJmol/JSmol

ダウンロードとリンク

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集合体

登録構造単位
R: RNA
T: Template strand DNA
N: Non-template strand DNA
A: DNA-directed RNA polymerase II subunit RPB1
B: DNA-directed RNA polymerase II subunit RPB2
C: DNA-directed RNA polymerase II subunit RPB3
E: DNA-directed RNA polymerases I, II, and III subunit RPABC1
F: DNA-directed RNA polymerases I, II, and III subunit RPABC2
H: DNA-directed RNA polymerases I, II, and III subunit RPABC3
I: DNA-directed RNA polymerase II subunit RPB9
J: DNA-directed RNA polymerases I, II, and III subunit RPABC5
K: DNA-directed RNA polymerase II subunit RPB11
L: DNA-directed RNA polymerases I, II, and III subunit RPABC4
ヘテロ分子


分子量 (理論値)分子数
合計 (水以外)488,03523
ポリマ－486,98013
非ポリマー1,05510
水00
1


  • 登録構造と同一
  • 登録者が定義した集合体
  • 根拠: gel filtration
タイプ名称対称操作数
identity operation1_555x,y,z1
単位格子
Length a, b, c (Å)159.784, 222.488, 192.287
Angle α, β, γ (deg.)90.00, 98.10, 90.00
Int Tables number5
Space group name H-MC121

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要素

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RNA鎖 , 1種, 1分子 R

#1: RNA鎖 RNA


分子量: 2934.831 Da / 分子数: 1 / 由来タイプ: 合成 / 由来: (合成) synthetic construct (人工物)

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DNA鎖 , 2種, 2分子 TN

#2: DNA鎖 Template strand DNA


分子量: 8755.594 Da / 分子数: 1 / 由来タイプ: 合成 / 由来: (合成) synthetic construct (人工物)
#3: DNA鎖 Non-template strand DNA


分子量: 5663.694 Da / 分子数: 1 / 由来タイプ: 合成 / 由来: (合成) synthetic construct (人工物)

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DNA-directed RNA polymerase II subunit ... , 5種, 5分子 ABCIK

#4: タンパク質 DNA-directed RNA polymerase II subunit RPB1 / RNA polymerase II subunit B1 / DNA-directed RNA polymerase III largest subunit / RNA polymerase II ...RNA polymerase II subunit B1 / DNA-directed RNA polymerase III largest subunit / RNA polymerase II subunit B220


分子量: 191821.578 Da / 分子数: 1 / 由来タイプ: 組換発現
由来: (組換発現) Saccharomyces cerevisiae S288C (パン酵母)
遺伝子: RPO21, RPB1, RPB220, SUA8, YDL140C, D2150 / 発現宿主: Saccharomyces cerevisiae S288C (パン酵母) / 参照: UniProt: P04050, DNA-directed RNA polymerase
#5: タンパク質 DNA-directed RNA polymerase II subunit RPB2 / RNA polymerase II subunit 2 / B150 / DNA-directed RNA polymerase II 140 kDa polypeptide


分子量: 138937.297 Da / 分子数: 1 / 由来タイプ: 組換発現
由来: (組換発現) Saccharomyces cerevisiae S288C (パン酵母)
遺伝子: RPB2, RPB150, RPO22, YOR151C / 発現宿主: Saccharomyces cerevisiae S288C (パン酵母) / 参照: UniProt: P08518, DNA-directed RNA polymerase
#6: タンパク質 DNA-directed RNA polymerase II subunit RPB3 / RNA polymerase II subunit B3 / B44.5 / DNA-directed RNA polymerase II 45 kDa polypeptide


分子量: 35330.457 Da / 分子数: 1 / 由来タイプ: 組換発現
由来: (組換発現) Saccharomyces cerevisiae S288C (パン酵母)
遺伝子: RPB3, YIL021W / 発現宿主: Saccharomyces cerevisiae S288C (パン酵母) / 参照: UniProt: P16370
#10: タンパク質 DNA-directed RNA polymerase II subunit RPB9 / RNA polymerase II subunit B9 / B12.6 / DNA-directed RNA polymerase II 14.2 kDa polypeptide / DNA- ...RNA polymerase II subunit B9 / B12.6 / DNA-directed RNA polymerase II 14.2 kDa polypeptide / DNA-directed RNA polymerase II subunit 9


分子量: 14308.161 Da / 分子数: 1 / 由来タイプ: 組換発現
由来: (組換発現) Saccharomyces cerevisiae S288C (パン酵母)
遺伝子: RPB9, YGL070C / 発現宿主: Saccharomyces cerevisiae S288C (パン酵母) / 参照: UniProt: P27999
#12: タンパク質 DNA-directed RNA polymerase II subunit RPB11 / RNA polymerase II subunit B11 / B13.6 / DNA-directed RNA polymerase II 13.6 kDa polypeptide


分子量: 13633.493 Da / 分子数: 1 / 由来タイプ: 組換発現
由来: (組換発現) Saccharomyces cerevisiae S288C (パン酵母)
遺伝子: RPB11, YOL005C / 発現宿主: Saccharomyces cerevisiae S288C (パン酵母) / 参照: UniProt: P38902

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DNA-directed RNA polymerases I, II, and III subunit ... , 5種, 5分子 EFHJL

#7: タンパク質 DNA-directed RNA polymerases I, II, and III subunit RPABC1 / RNA polymerases I / II / and III subunit ABC1 / ABC27 / DNA-directed RNA polymerases I / and III 27 ...RNA polymerases I / II / and III subunit ABC1 / ABC27 / DNA-directed RNA polymerases I / and III 27 kDa polypeptide


分子量: 25117.094 Da / 分子数: 1 / 由来タイプ: 組換発現
由来: (組換発現) Saccharomyces cerevisiae S288C (パン酵母)
遺伝子: RPB5, RPA7, RPC9, YBR154C, YBR1204 / 発現宿主: Saccharomyces cerevisiae S288C (パン酵母) / 参照: UniProt: P20434
#8: タンパク質 DNA-directed RNA polymerases I, II, and III subunit RPABC2 / RNA polymerases I / II / and III subunit ABC2 / ABC23 / DNA-directed RNA polymerases I / and III 23 ...RNA polymerases I / II / and III subunit ABC2 / ABC23 / DNA-directed RNA polymerases I / and III 23 kDa polypeptide


分子量: 17931.834 Da / 分子数: 1 / 由来タイプ: 組換発現
由来: (組換発現) Saccharomyces cerevisiae S288C (パン酵母)
遺伝子: RPO26, RPB6, YPR187W, P9677.8 / 発現宿主: Saccharomyces cerevisiae S288C (パン酵母) / 参照: UniProt: P20435
#9: タンパク質 DNA-directed RNA polymerases I, II, and III subunit RPABC3 / RNA polymerases I / II / and III subunit ABC3 / ABC14.4 / ABC14.5 / DNA-directed RNA polymerases I ...RNA polymerases I / II / and III subunit ABC3 / ABC14.4 / ABC14.5 / DNA-directed RNA polymerases I / and III 14.5 kDa polypeptide


分子量: 16525.363 Da / 分子数: 1 / 由来タイプ: 組換発現
由来: (組換発現) Saccharomyces cerevisiae S288C (パン酵母)
遺伝子: RPB8, YOR224C, YOR50-14 / 発現宿主: Saccharomyces cerevisiae S288C (パン酵母) / 参照: UniProt: P20436
#11: タンパク質 DNA-directed RNA polymerases I, II, and III subunit RPABC5 / RNA polymerases I / II / and III subunit ABC5 / ABC10-beta / ABC8 / DNA-directed RNA polymerases I ...RNA polymerases I / II / and III subunit ABC5 / ABC10-beta / ABC8 / DNA-directed RNA polymerases I / and III 8.3 kDa polypeptide


分子量: 8290.732 Da / 分子数: 1 / 由来タイプ: 組換発現
由来: (組換発現) Saccharomyces cerevisiae S288C (パン酵母)
遺伝子: RPB10, YOR210W / 発現宿主: Saccharomyces cerevisiae S288C (パン酵母) / 参照: UniProt: P22139
#13: タンパク質 DNA-directed RNA polymerases I, II, and III subunit RPABC4 / RNA polymerases I / II / and III subunit ABC4 / ABC10-alpha


分子量: 7729.969 Da / 分子数: 1 / 由来タイプ: 組換発現
由来: (組換発現) Saccharomyces cerevisiae S288C (パン酵母)
遺伝子: RPC10, RPB12, YHR143W-A, YHR143BW / 発現宿主: Saccharomyces cerevisiae S288C (パン酵母) / 参照: UniProt: P40422

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非ポリマー , 3種, 10分子

#14: 化合物 ChemComp-ATP / ADENOSINE-5'-TRIPHOSPHATE / ATP


分子量: 507.181 Da / 分子数: 1 / 由来タイプ: 合成 / 式: C10H16N5O13P3 / タイプ: SUBJECT OF INVESTIGATION / コメント: ATP, エネルギー貯蔵分子*YM
#15: 化合物
ChemComp-ZN / ZINC ION


分子量: 65.409 Da / 分子数: 8 / 由来タイプ: 合成 / 式: Zn
#16: 化合物 ChemComp-MG / MAGNESIUM ION / マグネシウムジカチオン


分子量: 24.305 Da / 分子数: 1 / 由来タイプ: 合成 / 式: Mg

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詳細

研究の焦点であるリガンドがあるかY
Has protein modificationY

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実験情報

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実験

実験手法: X線回折 / 使用した結晶の数: 1

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試料調製

結晶マシュー密度: 3.47 Å3/Da / 溶媒含有率: 64.6 %
結晶化温度: 298 K / 手法: 蒸気拡散法, ハンギングドロップ法
詳細: 390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000

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データ収集

回折平均測定温度: 100 K / Serial crystal experiment: N
放射光源由来: シンクロトロン / サイト: ALS / ビームライン: 5.0.3 / 波長: 0.9999 Å
検出器タイプ: DECTRIS PILATUS3 2M / 検出器: PIXEL / 日付: 2019年6月21日
放射プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長波長: 0.9999 Å / 相対比: 1
反射解像度: 3.15→48.06 Å / Num. obs: 114545 / % possible obs: 100 % / 冗長度: 7.7 % / CC1/2: 0.989 / Rmerge(I) obs: 0.378 / Rpim(I) all: 0.146 / Rrim(I) all: 0.405 / Χ2: 1.03 / Net I/σ(I): 5.4 / Num. measured all: 879890
反射 シェル解像度: 3.15→3.2 Å / % possible obs: 99.9 % / 冗長度: 7.7 % / Rmerge(I) obs: 3.489 / Num. measured all: 43765 / Num. unique obs: 5655 / CC1/2: 0.34 / Rpim(I) all: 1.344 / Rrim(I) all: 3.74 / Χ2: 0.97 / Net I/σ(I) obs: 0.6

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解析

ソフトウェア
名称バージョン分類
PHENIX1.21.2_5419精密化
Aimlessデータスケーリング
XDSデータ削減
PHENIX位相決定
精密化構造決定の手法: 分子置換 / 解像度: 3.15→48.06 Å / SU ML: 0.5 / 交差検証法: FREE R-VALUE / σ(F): 1.34 / 位相誤差: 29.54 / 立体化学のターゲット値: ML
Rfactor反射数%反射
Rfree0.2539 2000 1.75 %
Rwork0.2204 --
obs0.2209 114386 99.85 %
溶媒の処理減衰半径: 0.9 Å / VDWプローブ半径: 1.1 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL
精密化ステップサイクル: LAST / 解像度: 3.15→48.06 Å
タンパク質核酸リガンド溶媒全体
原子数27979 936 40 0 28955
拘束条件
Refine-IDタイプDev ideal数
X-RAY DIFFRACTIONf_bond_d0.00329620
X-RAY DIFFRACTIONf_angle_d0.63240243
X-RAY DIFFRACTIONf_dihedral_angle_d16.78811274
X-RAY DIFFRACTIONf_chiral_restr0.0444538
X-RAY DIFFRACTIONf_plane_restr0.0055037
LS精密化 シェル
解像度 (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.15-3.230.38711410.38687955X-RAY DIFFRACTION99
3.23-3.320.35671430.35768003X-RAY DIFFRACTION100
3.32-3.410.34361420.32457993X-RAY DIFFRACTION100
3.41-3.520.35771430.30288015X-RAY DIFFRACTION100
3.52-3.650.31371420.28737996X-RAY DIFFRACTION100
3.65-3.80.29921430.26928024X-RAY DIFFRACTION100
3.8-3.970.28371420.23737988X-RAY DIFFRACTION100
3.97-4.180.25111430.20718043X-RAY DIFFRACTION100
4.18-4.440.211430.1838039X-RAY DIFFRACTION100
4.44-4.780.21651420.1698004X-RAY DIFFRACTION100
4.78-5.260.2191440.17918079X-RAY DIFFRACTION100
5.26-6.020.2361430.20358037X-RAY DIFFRACTION100
6.02-7.580.22141440.21068074X-RAY DIFFRACTION100
7.58-48.060.20141450.16588136X-RAY DIFFRACTION100
精密化 TLS

手法: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
19.37324.06352.85053.2644-2.45069.9020.26290.6334-0.4158-0.6882-0.8555-2.3297-0.56512.32090.53011.3717-0.0910.22031.2775-0.0171.65914.922613.137538.7914
21.85472.81491.92814.38972.49844.1278-0.2795-0.16640.5732-0.1855-0.1453-0.9187-0.37521.39770.40782.03850.0390.13462.163-0.46332.843350.114812.540372.6192
34.8302-5.2274.51115.9597-5.06196.4947-1.0551-0.63791.0030.21640.2734-0.734-1.45620.07630.83221.5058-0.1835-0.12641.12530.04840.907520.026910.569341.3028
42.86420.37892.96030.87730.35323.0535-0.7035-0.74931.10990.56790.2172-0.7065-1.28080.72480.88891.8807-0.24240.02052.3747-0.49832.258945.015111.731769.714
51.7092-0.03090.4971.93220.56731.33610.04370.1310.53240.07780.0244-0.6678-0.13851.2981-0.05030.7478-0.311-0.12641.71050.11661.226455.6628.199738.681
61.41890.12110.22460.751-0.08361.8470.14660.31460.3315-0.0068-0.0563-0.19250.2420.3842-0.06380.76860.0663-0.1570.52060.08690.60522.155-4.066128.7469
70.52850.1441-0.01611.31680.08180.65570.0461-0.2117-0.21860.32440.030.02890.4853-0.0925-0.10671.2117-0.1818-0.23050.51590.14010.5833-7.4618-24.409855.5568
80.6363-0.41170.06170.29990.22011.18080.1855-0.3154-0.11910.228-0.0028-0.14090.2657-0.1432-0.18471.1094-0.1435-0.09080.47940.06150.51310.34873.147270.3573
90.62050.22430.02991.64-1.15391.1026-0.0109-0.1998-0.195-0.2830.29780.10130.66760.15870.25071.54060.1013-0.43440.58210.10760.701720.9087-40.450163.2422
100.7252-0.05550.78614.35261.93143.25650.0001-0.4580.27470.7556-0.22080.0789-0.1563-0.35190.15611.27980.0154-0.19820.7617-0.18650.753927.9176.735496.8399
110.91731.74291.35216.98544.03712.87660.0823-0.24640.17440.9425-0.11010.05470.14360.0122-0.00841.34370.1189-0.29670.8512-0.01420.606928.32741.132895.2832
121.0287-0.1155-0.25620.88270.18986.670.0016-0.02750.29560.14690.2308-0.1978-0.0140.803-0.13690.70830.0728-0.24180.5877-0.01280.627334.3621-11.172856.8676
131.74470.7140.13661.31830.06481.0271-0.1330.13460.5886-0.25080.09010.0966-0.58030.35760.0021.2193-0.1817-0.04420.42270.12620.75842.047243.697840.9769
142.6984-0.68744.28880.5-0.87966.9462-0.42640.13550.76230.0637-0.1039-0.2712-0.59820.58320.68520.8416-0.22560.04080.48240.0160.630612.804730.797555.0735
154.59891.1547-1.04392.4641-1.87334.10820.01060.00790.40.21960.0196-0.12280.03640.3069-0.01861.1253-0.2301-0.17530.5585-0.18460.699130.187340.320978.7556
160.5776-0.11310.2221.97120.07492.62730.3591-0.04910.04060.0885-0.3357-0.5984-0.09840.93290.05020.7727-0.2292-0.02720.62040.06190.737717.541536.575346.1168
170.81540.06150.36491.02970.5212.35360.017-0.0370.15370.04470.0961-0.1421-0.4013-0.0089-0.1010.5576-0.0363-0.00390.30840.05520.4513-4.026225.223553.5776
180.5208-0.2880.17350.67760.0151.18870.04760.20410.0841-0.0229-0.0637-0.17520.04950.2201-0.00220.6412-0.1002-0.07280.3840.0910.45338.1761.095232.6782
194.8431-2.6205-0.45649.4048-1.58466.1403-0.1025-0.1119-0.54280.05180.04691.09720.4285-0.49430.31190.406-0.26270.01960.99340.02480.5603-42.7795-16.424131.6792
203.65214.8592-1.10358.6427-1.93662.56050.11240.1873-0.39390.571-0.0766-0.5088-0.3491-0.23780.01690.6066-0.0193-0.17960.53040.03310.4542-22.56090.625821.1688
211.6650.64380.87191.87971.53483.69440.16840.37030.1388-0.6386-0.04950.67160.3965-0.4077-0.08170.79860.0993-0.18790.74860.07770.4518-27.76776.210713.7082
220.5104-0.1050.48071.8979-0.1753.8820.133-0.1789-0.1450.1667-0.00050.20340.58-0.7177-0.14660.804-0.3516-0.17230.81280.15230.6932-33.2053-18.861638.5781
237.1492-2.892-2.39335.0137-0.82085.29780.1331-1.5498-0.5812-0.4210.2220.98510.92341.592-0.47940.97630.0179-0.42051.5624-0.00620.873645.8601-21.72192.9014
241.0430.95710.06130.87560.12632.7649-0.2965-0.5807-0.12740.70050.0094-0.5703-0.16680.9887-0.47111.49860.561-0.63732.0863-0.23831.113254.4227-23.222892.7363
253.23610.6681-1.31810.5466-1.05482.03140.453-0.89830.37840.33630.1727-0.8106-0.06282.7497-0.56691.14780.0624-0.35722.4372-0.50421.233161.8593-10.789385.3885
262.16730.34840.81480.91171.52675.4826-0.4315-0.32350.14180.26460.2232-0.1872-0.00480.99490.12951.09350.2194-0.35240.8603-0.03440.649644.9731-22.384669.4563
275.3-0.3186-2.46290.03520.1841.225-0.041-0.1944-1.6867-0.8311-0.0977-2.42110.3031.1170.17271.61070.6195-0.58141.6638-0.16851.794352.109-31.529538.2922
282.06172.7354-0.11263.73680.45433.3911-0.0216-1.1052-1.43270.8750.4116-0.96291.68420.9773-0.68121.92220.3618-0.5480.91090.0690.998740.3829-39.700943.1857
295.2-0.13521.27254.51261.10364.47230.25820.7921-0.5393-1.01160.03150.36160.7912-0.0788-0.25141.20020.1741-0.18790.7291-0.03410.486823.6531-34.069627.0483
305.33180.0497-2.85282.8993-0.83181.77620.34890.6497-0.5673-0.4349-0.3268-0.2131-0.28480.762-0.13351.14580.17180.00361.0104-0.16530.569940.9474-29.882740.1196
316.30945.0806-1.45914.3658-2.58837.9809-0.09640.7396-1.1272-0.35870.1207-0.64681.53311.2725-0.24721.56930.3656-0.16470.7912-0.14780.703333.6579-38.086737.8512
322.8260.2035-0.21285.02330.17562.3041-0.1787-0.5951-0.38970.597-0.10930.04710.6189-0.12350.31160.9426-0.5676-0.06290.67150.47921.0633-23.9423-42.936466.4827
336.76184.4563-3.94144.2335-5.35248.1534-0.43241.4283-0.0917-1.67780.89310.80091.6731-0.716-0.18991.6653-0.5478-0.02080.83210.12081.008-24.3575-49.333343.8839
341.0559-1.4640.86452.0559-1.50664.25670.0369-0.57-1.2730.45060.10070.09020.53120.1237-0.03871.5185-0.3485-0.21130.76510.45241.1904-15.0542-49.051762.474
350.41050.7129-0.40324.8877-0.69030.7894-0.0138-0.11020.06790.8136-0.0652-0.1823-0.52420.20110.05611.7194-0.0424-0.25370.8579-0.31260.926531.977437.9813102.6888
365.5816-1.3653-1.93496.06223.51494.4918-0.1649-0.7659-0.1992-0.3581-0.04910.0337-0.10130.99040.1210.5901-0.0252-0.04160.6268-0.01020.4646-3.20427.776292.6439
378.7279-5.5419-1.40484.1455-0.37936.0596-0.4031-0.8030.33520.45680.8936-1.32870.6733-0.089-0.50531.3086-0.0423-0.30680.7405-0.1050.77874.832221.623795.0565
380.77970.45170.75821.3658-0.12141.13770.0354-0.23910.0880.1581-0.36350.13110.1732-0.2521-0.35560.1257-0.09780.05390.41470.07970.2311-25.31846.449830.2862
390.5010.51660.31110.9931-0.22620.85170.0785-0.28830.23860.30670.04220.7119-0.0981-0.4781-0.04860.1398-0.0090.17320.71860.06060.5597-35.816614.345437.3461
400.348-0.15790.19270.3121-0.010.1650.1866-0.04320.11270.04040.03440.1188-0.13630.04140.48610.15030.01690.19040.50450.08120.3994-25.372110.038337.8258
412.0506-0.65881.09448.98492.45174.54150.03440.15341.3369-0.06850.0295-0.231-0.67140.5604-0.1650.3447-0.04510.04380.57630.09780.6181-18.0427.419927.2578
420.83141.46660.33714.91820.52750.4629-0.17250.3482-0.10190.01280.1199-0.7664-0.0084-0.52710.38911.26670.0301-0.3490.82840.07810.6685-8.8162-8.611520.406
435.099-2.22122.45358.5622-6.04699.03860.7338-0.3208-0.4321-0.9574-0.6790.2670.5240.6508-0.25331.2314-0.0974-0.28880.7078-0.11680.4429-12.941-29.316921.0303
445.0824-2.17020.66851.97152.09715.5252-0.00930.0994-0.42720.2135-0.38371.04190.4081-0.76130.53691.1654-0.3955-0.4150.64560.09270.8095-24.8261-28.208530.1752
453.73333.8319-0.84657.3213-0.46330.24310.45540.0963-0.9798-0.2286-0.3392-0.07350.695-0.40820.0791.3045-0.2701-0.33980.5742-0.02350.7504-16.9667-32.772428.8969
463.0024-1.60710.99442.223-0.36911.88280.53520.5026-0.69270.0019-0.24590.73010.8418-0.9567-0.05950.6964-0.4116-0.06040.980.04740.9157-38.0769-23.874723.775
476.951.7632.30472.43590.14917.7405-0.4223-0.64812.3841-1.36170.2041-0.1409-2.0415-1.07540.16252.61250.3405-0.62240.9593-0.15341.5106-7.273832.758610.9681
482.08970.4767-0.1020.3178-0.29840.5597-0.52050.8107-1.1146-0.2335-0.00790.5841.3707-0.79120.53141.7891-0.70350.51012.2649-0.15741.79721.299835.83336.6058
495.07263.30520.04272.16640.34098.1656-0.81170.14581.52810.79030.19350.1666-2.477-1.60160.26931.94330.1224-0.55531.46740.54441.1558-3.45135.829113.9187
505.97092.3834-2.57923.17080.62593.6601-0.78291.79560.9079-1.38960.24470.3626-0.3991-0.38360.42461.00680.0657-0.26711.10390.08290.5082-11.291611.319810.4171
精密化 TLSグループ
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'R' and (resid 1 through 9 )
2X-RAY DIFFRACTION2chain 'T' and (resid 5 through 14 )
3X-RAY DIFFRACTION3chain 'T' and (resid 15 through 28 )
4X-RAY DIFFRACTION4chain 'N' and (resid 3 through 14 )
5X-RAY DIFFRACTION5chain 'A' and (resid 3 through 260 )
6X-RAY DIFFRACTION6chain 'A' and (resid 261 through 511 )
7X-RAY DIFFRACTION7chain 'A' and (resid 512 through 699 )
8X-RAY DIFFRACTION8chain 'A' and (resid 700 through 844 )
9X-RAY DIFFRACTION9chain 'A' and (resid 845 through 1078 )
10X-RAY DIFFRACTION10chain 'A' and (resid 1079 through 1220 )
11X-RAY DIFFRACTION11chain 'A' and (resid 1221 through 1317 )
12X-RAY DIFFRACTION12chain 'A' and (resid 1318 through 1446 )
13X-RAY DIFFRACTION13chain 'B' and (resid 20 through 191 )
14X-RAY DIFFRACTION14chain 'B' and (resid 192 through 251 )
15X-RAY DIFFRACTION15chain 'B' and (resid 252 through 389 )
16X-RAY DIFFRACTION16chain 'B' and (resid 390 through 487 )
17X-RAY DIFFRACTION17chain 'B' and (resid 488 through 902 )
18X-RAY DIFFRACTION18chain 'B' and (resid 903 through 1221 )
19X-RAY DIFFRACTION19chain 'C' and (resid 2 through 26 )
20X-RAY DIFFRACTION20chain 'C' and (resid 27 through 69 )
21X-RAY DIFFRACTION21chain 'C' and (resid 70 through 175 )
22X-RAY DIFFRACTION22chain 'C' and (resid 176 through 268 )
23X-RAY DIFFRACTION23chain 'E' and (resid 3 through 38 )
24X-RAY DIFFRACTION24chain 'E' and (resid 39 through 81 )
25X-RAY DIFFRACTION25chain 'E' and (resid 82 through 130 )
26X-RAY DIFFRACTION26chain 'E' and (resid 131 through 215 )
27X-RAY DIFFRACTION27chain 'F' and (resid 69 through 73 )
28X-RAY DIFFRACTION28chain 'F' and (resid 74 through 83 )
29X-RAY DIFFRACTION29chain 'F' and (resid 84 through 132 )
30X-RAY DIFFRACTION30chain 'F' and (resid 133 through 142 )
31X-RAY DIFFRACTION31chain 'F' and (resid 143 through 154 )
32X-RAY DIFFRACTION32chain 'H' and (resid 2 through 60 )
33X-RAY DIFFRACTION33chain 'H' and (resid 61 through 92 )
34X-RAY DIFFRACTION34chain 'H' and (resid 93 through 146 )
35X-RAY DIFFRACTION35chain 'I' and (resid 2 through 52 )
36X-RAY DIFFRACTION36chain 'I' and (resid 53 through 87 )
37X-RAY DIFFRACTION37chain 'I' and (resid 88 through 119 )
38X-RAY DIFFRACTION38chain 'J' and (resid 1 through 14 )
39X-RAY DIFFRACTION39chain 'J' and (resid 15 through 43 )
40X-RAY DIFFRACTION40chain 'J' and (resid 44 through 56 )
41X-RAY DIFFRACTION41chain 'J' and (resid 57 through 65 )
42X-RAY DIFFRACTION42chain 'K' and (resid 1 through 9 )
43X-RAY DIFFRACTION43chain 'K' and (resid 10 through 39 )
44X-RAY DIFFRACTION44chain 'K' and (resid 40 through 52 )
45X-RAY DIFFRACTION45chain 'K' and (resid 53 through 82 )
46X-RAY DIFFRACTION46chain 'K' and (resid 83 through 114 )
47X-RAY DIFFRACTION47chain 'L' and (resid 28 through 38 )
48X-RAY DIFFRACTION48chain 'L' and (resid 39 through 48 )
49X-RAY DIFFRACTION49chain 'L' and (resid 49 through 58 )
50X-RAY DIFFRACTION50chain 'L' and (resid 59 through 70 )

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万見について

-
お知らせ

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2022年2月9日: EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

  • EMDBのヘッダファイルのバージョン3が、公式のフォーマットとなりました。
  • これまでは公式だったバージョン1.9は、アーカイブから削除されます。

関連情報:EMDBヘッダ

外部リンク:wwPDBはEMDBデータモデルのバージョン3へ移行します

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2020年8月12日: 新型コロナ情報

新型コロナ情報

URL: https://pdbj.org/emnavi/covid19.php

新ページ: EM Navigatorに新型コロナウイルスの特設ページを開設しました。

関連情報:Covid-19情報 / 2020年3月5日: 新型コロナウイルスの構造データ

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2020年3月5日: 新型コロナウイルスの構造データ

新型コロナウイルスの構造データ

関連情報:万見生物種 / 2020年8月12日: 新型コロナ情報

外部リンク:COVID-19特集ページ - PDBj / 今月の分子2020年2月：コロナウイルスプロテーアーゼ

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2019年1月31日: EMDBのIDの桁数の変更

EMDBのIDの桁数の変更

  • EMDBエントリに付与されているアクセスコード(EMDB-ID)は4桁の数字(例、EMD-1234)でしたが、間もなく枯渇します。これまでの4桁のID番号は4桁のまま変更されませんが、4桁の数字を使い切った後に発行されるIDは5桁以上の数字(例、EMD-12345)になります。5桁のIDは2019年の春頃から発行される見通しです。
  • EM Navigator/万見では、接頭語「EMD-」は省略されています。

関連情報:Q: 「EMD」とは何ですか？ / 万見/EM NavigatorにおけるID/アクセスコードの表記

外部リンク:EMDB Accession Codes are Changing Soon! / PDBjへお問い合わせ

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2017年7月12日: PDB大規模アップデート

PDB大規模アップデート

  • 新バージョンのPDBx/mmCIF辞書形式に基づくデータがリリースされました。
  • 今回の更新はバージョン番号が4から5になる大規模なもので、全エントリデータの書き換えが行われる「Remediation」というアップデートに該当します。
  • このバージョンアップで、電子顕微鏡の実験手法に関する多くの項目の書式が改定されました(例：em_softwareなど)。
  • EM NavigatorとYorodumiでも、この改定に基づいた表示内容になります。

外部リンク:wwPDB Remediation / OneDepデータ基準に準拠した、より強化された内容のモデル構造ファイルが、PDBアーカイブで公開されました。

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万見 (Yorodumi)

幾万の構造データを、幾万の視点から

  • 万見(Yorodumi)は、EMDB/PDB/SASBDBなどの構造データを閲覧するためのページです。
  • EM Navigatorの詳細ページの後継、Omokage検索のフロントエンドも兼ねています。

関連情報:EMDB / PDB / SASBDB / 3つのデータバンクの比較 / 万見検索 / 2016年8月31日: 新しいEM Navigatorと万見 / 万見文献 / Jmol/JSmol / 機能・相同性情報 / 新しいEM Navigatorと万見の変更点

他の情報も見る