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- PDB-9mzt: F pilus filament from conjugative plasmid pED208 -

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Basic information

Entry
Database: PDB / ID: 9mzt
TitleF pilus filament from conjugative plasmid pED208
ComponentsPilin
KeywordsPROTEIN FIBRIL / Conjugative pili from pED208
Function / homologyTraA / TraA / extracellular region / plasma membrane / 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / Pilin
Function and homology information
Biological speciesEscherichia coli (E. coli)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.11 Å
AuthorsLea, S. / Deme, J. / Lungren, C.A.K.
Funding support United States, 1items
OrganizationGrant numberCountry
Other government United States
CitationJournal: To Be Published
Title: F pilus filament from conjugative plasmid pED208
Authors: Lea, S. / Deme, J. / Lungren, C.A.K.
History
DepositionJan 23, 2025Deposition site: RCSB / Processing site: RCSB
Revision 1.0Apr 16, 2025Provider: repository / Type: Initial release
Revision 1.0Apr 16, 2025Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release
Revision 1.0Apr 16, 2025Data content type: Additional map / Part number: 1 / Data content type: Additional map / Provider: repository / Type: Initial release
Revision 1.0Apr 16, 2025Data content type: FSC / Data content type: FSC / Provider: repository / Type: Initial release
Revision 1.0Apr 16, 2025Data content type: Half map / Part number: 1 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0Apr 16, 2025Data content type: Half map / Part number: 2 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0Apr 16, 2025Data content type: Image / Data content type: Image / Provider: repository / Type: Initial release
Revision 1.0Apr 16, 2025Data content type: Primary map / Data content type: Primary map / Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Pilin
B: Pilin
C: Pilin
D: Pilin
E: Pilin
F: Pilin
G: Pilin
H: Pilin
I: Pilin
J: Pilin
K: Pilin
L: Pilin
M: Pilin
N: Pilin
O: Pilin
P: Pilin
Q: Pilin
R: Pilin
S: Pilin
T: Pilin
U: Pilin
V: Pilin
W: Pilin
X: Pilin
Y: Pilin
Z: Pilin
a: Pilin
b: Pilin
c: Pilin
d: Pilin
e: Pilin
f: Pilin
g: Pilin
h: Pilin
i: Pilin
j: Pilin
k: Pilin
l: Pilin
m: Pilin
n: Pilin
o: Pilin
p: Pilin
q: Pilin
r: Pilin
s: Pilin
t: Pilin
u: Pilin
v: Pilin
w: Pilin
x: Pilin
y: Pilin
z: Pilin
FA: Pilin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)403,667106
Polymers365,35053
Non-polymers38,31753
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_5551
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1chain "C"
d_2ens_1chain "b"
d_3ens_1chain "c"
d_4ens_1chain "d"
d_5ens_1chain "e"
d_6ens_1chain "f"
d_7ens_1chain "g"
d_8ens_1chain "h"
d_9ens_1chain "i"
d_10ens_1chain "j"
d_11ens_1chain "A"
d_12ens_1chain "a"
d_13ens_1chain "B"
d_14ens_1chain "D"
d_15ens_1chain "E"
d_16ens_1chain "F"
d_17ens_1chain "G"
d_18ens_1chain "H"
d_19ens_1chain "I"
d_20ens_1chain "J"
d_21ens_1chain "K"
d_22ens_1chain "L"
d_23ens_1chain "M"
d_24ens_1chain "N"
d_25ens_1chain "O"
d_26ens_1chain "P"
d_27ens_1chain "Q"
d_28ens_1chain "R"
d_29ens_1chain "S"
d_30ens_1chain "T"
d_31ens_1chain "U"
d_32ens_1chain "V"
d_33ens_1chain "W"
d_34ens_1chain "X"
d_35ens_1chain "Y"
d_36ens_1chain "Z"
d_37ens_1chain "k"
d_38ens_1chain "l"
d_39ens_1chain "m"
d_40ens_1chain "n"
d_41ens_1chain "o"
d_42ens_1chain "p"
d_43ens_1chain "q"
d_44ens_1chain "r"
d_45ens_1chain "s"
d_46ens_1chain "t"
d_47ens_1chain "u"
d_48ens_1chain "v"
d_49ens_1chain "w"
d_50ens_1chain "x"
d_51ens_1chain "y"
d_52ens_1chain "z"

NCS oper:
IDCodeMatrixVector
1given(-0.777310249632, 0.629117457883, 1.9297966582E-6), (-0.629117457886, -0.777310249628, -2.61751985335E-6), (-1.46676713985E-7, -3.24869377847E-6, 0.999999999995)215.244250324, 451.034715119, -60.429425614
2given(0.259364895404, -0.965779369851, -0.000244543637469), (0.965779386422, 0.259364926935, -0.000106950045162), (0.000166716189886, -0.000208436116871, 0.99999996438)320.010689804, -42.2196025059, -60.4307614519
3given(0.358198089785, 0.933645610789, 4.39176776574E-5), (-0.933645611355, 0.358198087721, 4.84821910068E-5), (2.95339566809E-5, -5.83697752129E-5, 0.99999999786)-54.6878667615, 295.212577149, -60.4248785138
4given(0.596107717562, 0.802904445901, 0.000199536109115), (-0.802904457106, 0.596107734789, -3.5840892052E-5), (-0.000147721829586, -0.000138843399005, 0.99999997945)-74.8410777425, 226.105523805, -84.5476146719
5given(-0.480423697147, -0.877036487645, 0.000265628181509), (0.877033904448, -0.480422978845, -0.00230040158028), (0.00214515000437, -0.000872202510963, 0.999997318794)441.827410628, 113.718136176, -72.8171027246
6given(-0.982346573322, 0.187070066737, -3.72301335347E-6), (-0.187070066745, -0.982346573325, 1.97718573145E-6), (-3.2874171435E-6, 2.63874598464E-6, 0.999999999991)336.529061148, 406.600167441, -72.5157515524
7given(0.684997308507, -0.728545547103, -0.000270434045149), (0.728545578182, 0.684997328372, 2.52027794475E-5), (0.000166885225687, -0.000214287363871, 0.999999963115)195.746195737, -77.6056353531, -72.5158178317
8given(-0.125555149229, 0.99208664025, 5.23376975992E-5), (-0.992086641055, -0.12555515078, 2.7455701142E-5), (3.38097018152E-5, -4.84763259576E-5, 0.999999998253)25.0009390038, 396.84562177, -72.5134080498
9given(0.145891897051, 0.989300525261, 0.000158409032147), (-0.989300526538, 0.145891917813, -0.00012848534638), (-0.000150221218161, -0.000137969167985, 0.999999979199)-25.4205324808, 345.470173646, -96.6332463089
10given(-0.435912645382, -0.899987406544, -0.00168334767315), (0.899988980799, -0.435911870342, -0.000822030860528), (6.02618962872E-6, -0.00187332800368, 0.999998245301)438.368039776, 100.756902689, -11.7685983941
11given(-0.837868122589, -0.545871109057, 0.00131963908885), (0.545868484253, -0.837868702747, -0.00190652977039), (0.00214640381168, -0.000877071130185, 0.999997311845)446.459931006, 242.687037236, -60.7305650178
12given(0.307864232383, 0.951430282809, 0.00017712676106), (-0.951427892497, 0.307863029506, 0.00230660847652), (0.00214004637388, -0.000878645589013, 0.999997324088)-48.6515999778, 307.414288442, -0.298988488275
13given(-0.809198158595, 0.58753576397, 0.000257258425461), (-0.587535796747, -0.809198172557, -7.12132840374E-5), (0.000166332696516, -0.000208774192283, 0.999999964373)228.879908616, 449.320921701, -0.000627449248555
14given(0.308937274636, -0.951082412528, -7.01401573879E-5), (0.951082414616, 0.308937276023, -9.60653390079E-6), (3.08055146016E-5, -6.3741253848E-5, 0.999999997494)307.854165286, -48.697394085, 0.00575442808326
15given(0.0417559660139, -0.999127830448, -0.000133139700341), (0.999127828286, 0.0417559453791, 0.000154172947187), (-0.000148479108181, -0.000139461220004, 0.999999979252)366.994259187, -7.65837960215, -24.117356474
16given(-0.178322650258, 0.983971287591, 0.00124000103714), (-0.983969731991, -0.17832483168, 0.00195472049639), (0.00214451181994, -0.000871552548754, 0.999997320729)36.0873117214, 404.746634537, -12.3870832741
17given(0.881280563703, 0.472593448992, -3.08343449727E-6), (-0.472593449002, 0.881280563688, -5.09293924658E-6), (3.10481167801E-7, 5.94551931397E-6, 0.999999999982)-66.3196742707, 110.839256996, -12.0870451573
18given(-0.990794717487, 0.13537277618, 0.000198166124985), (-0.135372813333, -0.99079471442, -0.000187852208873), (0.000170911874186, -0.000212949282066, 0.999999962721)347.729593259, 398.651524365, -12.0868867231
19given(0.721737767736, -0.692166591738, -6.24960494758E-5), (0.692166593797, 0.721737768236, 1.82455029409E-5), (3.24768316871E-5, -5.64261462552E-5, 0.999999997881)181.969419779, -77.5663670579, -12.0818202228
20given(0.50898204939, -0.860777132421, -4.12366004652E-5), (0.860777120725, 0.508982033082, 0.000196052197092), (-0.000147768559276, -0.00013528257128, 0.999999979932)253.483592606, -69.3498299367, -36.2042049718
21given(-0.622174236185, 0.782876200398, 0.00201858332621), (-0.782875873, -0.622176893667, 0.00113157454109), (0.0021417986807, -0.000876263657941, 0.999997322427)156.764057675, 450.480135233, -24.4717485897
22given(0.553309053755, 0.832976044692, 1.06793981918E-6), (-0.832976044686, 0.553309053755, -3.52685064177E-6), (-3.52868286861E-6, 1.06187010479E-6, 0.999999999993)-72.386485956, 239.861216756, -24.1714327753
23given(-0.937141035159, -0.348950818408, 8.09492574408E-5), (0.348950788057, -0.93714101393, -0.000259855490539), (0.000166537655286, -0.000215273936219, 0.999999962961)428.529374002, 297.824624099, -24.1715792492
24given(0.963165595399, -0.268908969058, -4.69053851385E-5), (0.268908971157, 0.963165594844, 4.62671683798E-5), (3.2735996628E-5, -5.71762236393E-5, 0.99999999783)57.3894197332, -43.518451931, -24.1677468655
25given(0.855356092946, -0.518040491935, 5.4544726168E-5), (0.518040473515, 0.855356083238, 0.000196642697884), (-0.000148524043284, -0.000139943153997, 0.999999979178)124.294729745, -70.0746119822, -48.2892704871
26given(-0.918288612075, 0.395904875064, 0.00231405131172), (-0.395905827322, -0.918291115794, 5.04687839647E-5), (0.00214495359866, -0.000869801489452, 0.999997321306)284.724482161, 433.750523661, -36.5594634235
27given(0.0939560122925, 0.995576349535, -2.34736872602E-7), (-0.995576349535, 0.0939560122925, 2.81443217033E-7), (3.02253151103E-7, 2.07255196367E-7, 1)-16.7556646856, 356.432111994, -36.258097219
28given(-0.66097106181, -0.75041138945, -4.5097123399E-5), (0.750411372274, -0.660971030938, -0.000261957851386), (0.000166768263091, -0.000206987953436, 0.999999964672)452.082252303, 170.78269676, -36.2590177491
29given(0.975900057966, 0.218217956533, -1.75870414202E-5), (-0.21821795504, 0.975900056398, 6.3407967177E-5), (3.09999517391E-5, -5.80420306296E-5, 0.999999997835)-36.3134200313, 45.3677274539, -36.2532407548
30given(0.998631288888, -0.052302283616, 0.000141358893263), (0.0523022628404, 0.99863128918, 0.000146877434786), (-0.000148847439067, -0.000139283012019, 0.999999979222)10.100987464, -9.65818267253, -60.3753155247
31given(-0.996370256234, -0.0851003327215, 0.00206054926727), (0.0850982937047, -0.996371999381, -0.0010579500206), (0.00214310549201, -0.000878760706365, 0.999997317436)389.594046861, 358.531833672, -48.6436031213
32given(-0.387710060689, 0.921781378007, -8.2263477289E-7), (-0.921781378007, -0.387710060688, 3.55821615286E-7), (9.04596114163E-9, 8.96245034608E-7, 1)87.3633638155, 432.87391095, -48.3441476634
33given(-0.227849969698, -0.973696237139, -0.000170559713916), (0.973696237945, -0.227849933052, -0.000210283349983), (0.000165890087214, -0.000213986406706, 0.999999963345)412.801930193, 47.6916972434, -48.3436656686
34given(0.756913591681, 0.653514968921, 1.1169514257E-5), (-0.653514968292, 0.756913589926, 6.00239289519E-5), (3.07721789296E-5, -5.27323724052E-5, 0.999999998136)-76.8814030902, 167.982078856, -48.3400815437
35given(0.904794330432, 0.425848779533, 0.000191282103091), (-0.425848796876, 0.904794340528, 5.95567578477E-5), (-0.000147708791679, -0.000135343870305, 0.999999979932)-61.9820078027, 97.5520973973, -72.462203362
36given(-0.00888871203025, -0.999960124918, -0.000859867608644), (0.999958201218, -0.00888684668654, -0.00214936589691), (0.00214163867916, -0.00087893676173, 0.999997320423)376.794157545, 2.2464602808, -84.9012725492
37given(-0.95413088628, -0.299389799169, -9.27592675447E-7), (0.29938979916, -0.954130886268, 5.29073234264E-6), (-2.46903611504E-6, 4.77033935435E-6, 0.999999999986)422.412372787, 310.124598401, -84.6022787201
38given(0.947978257637, -0.318334998402, -0.000227685134333), (0.318335034521, 0.947978261835, 0.000144513482374), (0.00016983685871, -0.000209475794324, 0.999999963638)69.5130422546, -50.0625815182, -84.6032344246
39given(-0.579504690633, 0.814968900894, 6.41172867912E-5), (-0.814968902709, -0.579504691614, -3.93184906242E-6), (3.39519338002E-5, -5.45321198354E-5, 0.999999997937)143.258103485, 448.755913355, -84.5984306729
40given(-0.338958600798, 0.940801287463, 6.67298560421E-5), (-0.940801278411, -0.338958584781, -0.000179840821262), (-0.000146575818615, -0.000123738127014, 0.999999981602)74.5456359866, 427.304532402, -108.722363975
41given(0.464732271531, -0.885449477668, -0.00177152333816), (0.885448653338, 0.464734823791, -0.00149193057219), (0.00214431773226, -0.000875244670345, 0.999997317921)266.804374478, -65.2513902459, -96.9884317727
42given(-0.699361340948, -0.714768294465, -3.89621740253E-6), (0.714768294471, -0.699361340953, -3.69252078166E-7), (-2.46093414914E-6, -3.04313329623E-6, 0.999999999992)452.507818174, 184.514693994, -96.6869690424
43given(0.985876957975, 0.1674712139, -0.000127467595114), (-0.167471181516, 0.985876944301, 0.000232503882726), (0.000164605070645, -0.00020787307186, 0.999999964847)-28.7214104784, 33.8690980042, -96.688410534
44given(-0.895856556581, 0.444343366763, 4.94432492248E-5), (-0.444343368499, -0.895856556511, -3.20681933696E-5), (3.00447699854E-5, -5.06982811979E-5, 0.999999998263)272.011676694, 438.614034847, -96.6842721535
45given(-0.743345507508, 0.668907658885, -1.87706774116E-5), (-0.668907642265, -0.743345494758, -0.000203820191558), (-0.000150289985656, -0.000138952974163, 0.999999979052)201.31878172, 452.186690837, -120.805067849
46given(0.828018204763, -0.560696630025, -0.00226752286608), (0.560697110157, 0.828020846517, -0.000477906884108), (0.00214551698246, -0.000875677918004, 0.999997314969)137.97136234, -72.7568403256, -109.074603353
47given(-0.278537963065, -0.960425219958, 1.95959171147E-7), (0.960425219957, -0.278537963064, 1.34369746092E-6), (-1.23593886109E-6, 5.62474883792E-7, 0.999999999999)419.66512531, 59.5946034977, -108.773837741
48given(0.789677089347, 0.61352269273, 8.05536180323E-6), (-0.613522672111, 0.789677059264, 0.000269957989912), (0.000159264218475, -0.000218121786818, 0.999999963529)-75.6290211821, 154.262955751, -108.771583775
49given(-0.999494219558, -0.0318010076075, 3.14011141048E-5), (0.0318010057433, -0.999494218417, -5.81793563774E-5), (3.32353941544E-5, -5.71513433868E-5, 0.999999997815)380.686009604, 368.827508864, -108.769852617
50given(-0.971215562119, 0.238202264665, -0.000114048127954), (-0.238202243941, -0.971215559018, -0.00017001070259), (-0.00015126225072, -0.000137950520086, 0.999999979045)324.797272155, 414.200163561, -132.891033647
51given(0.994699093295, -0.102804527047, -0.00222328944559), (0.10280619275, 0.994701194702, 0.000648066537554), (0.00214488449381, -0.000873199120584, 0.999997318493)20.8845629652, -18.4833987199, -121.160881312

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Components

#1: Protein ...
Pilin


Mass: 6893.391 Da / Num. of mol.: 53 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P12060
#2: Chemical...
ChemComp-LHG / 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE


Mass: 722.970 Da / Num. of mol.: 53 / Source method: obtained synthetically / Formula: C38H75O10P / Comment: phospholipid*YM
Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: HELICAL ARRAY / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Complex of an assembled conjugative pili from plasmid pED208
Type: COMPLEX / Entity ID: #1 / Source: NATURAL
Source (natural)Organism: Escherichia coli (E. coli)
Buffer solutionpH: 7.15
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 800 nm
Image recordingElectron dose: 54 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k)

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Processing

EM software
IDNameVersionCategory
2PHENIXdev_5430model refinement
5SIMPLECTF correction
10cryoSPARCinitial Euler assignment
11cryoSPARCfinal Euler assignment
13cryoSPARC3D reconstruction
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 2.11 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 638176
Details: helical reconstruction with cryoSPARC Helical Twist: 28.203 degrees Helical Rise: 12.086 Angstroms Helical Symmetry Order: 6.000
Symmetry type: POINT
RefinementCross valid method: NONE
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
Displacement parametersBiso mean: 38.61 Å2
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.00728302
ELECTRON MICROSCOPYf_angle_d0.698437736
ELECTRON MICROSCOPYf_chiral_restr0.05024876
ELECTRON MICROSCOPYf_plane_restr0.00664187
ELECTRON MICROSCOPYf_dihedral_angle_d26.02425991
Refine LS restraints NCS
Ens-IDDom-IDAsym-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2IBDELECTRON MICROSCOPYNCS constraints3.48295954389E-12
ens_1d_3IBDELECTRON MICROSCOPYNCS constraints6.58818821699E-13
ens_1d_4IBDELECTRON MICROSCOPYNCS constraints9.74325907236E-11
ens_1d_5IBDELECTRON MICROSCOPYNCS constraints2.05452273599E-13
ens_1d_6IBDELECTRON MICROSCOPYNCS constraints2.90997516252E-10
ens_1d_7IBDELECTRON MICROSCOPYNCS constraints2.4283256067E-12
ens_1d_8IBDELECTRON MICROSCOPYNCS constraints5.9140304825E-11
ens_1d_9IBDELECTRON MICROSCOPYNCS constraints1.08262808392E-10
ens_1d_10IBDELECTRON MICROSCOPYNCS constraints2.10593472638E-10
ens_1d_11IBDELECTRON MICROSCOPYNCS constraints2.30866114303E-13
ens_1d_12IBDELECTRON MICROSCOPYNCS constraints2.42697331204E-13
ens_1d_13IBDELECTRON MICROSCOPYNCS constraints2.10284661375E-10
ens_1d_14IBDELECTRON MICROSCOPYNCS constraints6.45647762735E-13
ens_1d_15IBDELECTRON MICROSCOPYNCS constraints8.47561038407E-13
ens_1d_16IBDELECTRON MICROSCOPYNCS constraints2.6121243119E-13
ens_1d_17IBDELECTRON MICROSCOPYNCS constraints5.56936893743E-12
ens_1d_18IBDELECTRON MICROSCOPYNCS constraints2.08856057166E-13
ens_1d_19IBDELECTRON MICROSCOPYNCS constraints3.94337944049E-10
ens_1d_20IBDELECTRON MICROSCOPYNCS constraints2.77607529286E-13
ens_1d_21IBDELECTRON MICROSCOPYNCS constraints2.58815840989E-10
ens_1d_22IBDELECTRON MICROSCOPYNCS constraints1.26806182478E-13
ens_1d_23IBDELECTRON MICROSCOPYNCS constraints3.5268472875E-13
ens_1d_24IBDELECTRON MICROSCOPYNCS constraints1.22913454719E-13
ens_1d_25IBDELECTRON MICROSCOPYNCS constraints3.02743098756E-13
ens_1d_26IBDELECTRON MICROSCOPYNCS constraints1.06337933064E-12
ens_1d_27IBDELECTRON MICROSCOPYNCS constraints2.51854075475E-10
ens_1d_28IBDELECTRON MICROSCOPYNCS constraints2.58896542267E-10
ens_1d_29IBDELECTRON MICROSCOPYNCS constraints5.33768323409E-10
ens_1d_30IBDELECTRON MICROSCOPYNCS constraints4.85286007165E-12
ens_1d_31IBDELECTRON MICROSCOPYNCS constraints1.87549339532E-12
ens_1d_32IBDELECTRON MICROSCOPYNCS constraints3.06925120884E-13
ens_1d_33IBDELECTRON MICROSCOPYNCS constraints3.25355108221E-13
ens_1d_34IBDELECTRON MICROSCOPYNCS constraints2.27834859791E-13
ens_1d_35IBDELECTRON MICROSCOPYNCS constraints9.92149744361E-13
ens_1d_36IBDELECTRON MICROSCOPYNCS constraints1.23099549886E-10
ens_1d_37IBDELECTRON MICROSCOPYNCS constraints3.5637021983E-11
ens_1d_38IBDELECTRON MICROSCOPYNCS constraints4.35030901131E-13
ens_1d_39IBDELECTRON MICROSCOPYNCS constraints1.47971677502E-13
ens_1d_40IBDELECTRON MICROSCOPYNCS constraints4.91921393175E-13
ens_1d_41IBDELECTRON MICROSCOPYNCS constraints1.28029949444E-13
ens_1d_42IBDELECTRON MICROSCOPYNCS constraints3.41227737408E-13
ens_1d_43IBDELECTRON MICROSCOPYNCS constraints2.74418248737E-10
ens_1d_44IBDELECTRON MICROSCOPYNCS constraints2.12191255214E-10
ens_1d_45IBDELECTRON MICROSCOPYNCS constraints1.03914717858E-11
ens_1d_46IBDELECTRON MICROSCOPYNCS constraints2.65152500467E-11
ens_1d_47IBDELECTRON MICROSCOPYNCS constraints8.59329123757E-13
ens_1d_48IBDELECTRON MICROSCOPYNCS constraints1.38464783423E-10
ens_1d_49IBDELECTRON MICROSCOPYNCS constraints5.98240891271E-12
ens_1d_50IBDELECTRON MICROSCOPYNCS constraints5.16009788789E-13
ens_1d_51IBDELECTRON MICROSCOPYNCS constraints4.82433320225E-13
ens_1d_52IBDELECTRON MICROSCOPYNCS constraints3.00132411203E-12

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