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- PDB-9m7q: Structure of flagellar hook at 3.18 angstroms resolution,conforma... -

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Basic information

Entry
Database: PDB / ID: 9m7q
TitleStructure of flagellar hook at 3.18 angstroms resolution,conformation 1.
ComponentsFlagellar hook protein FlgE
KeywordsPROTEIN FIBRIL / flagellum / hook / FlgE
Function / homology
Function and homology information


bacterial-type flagellum hook / bacterial-type flagellum basal body / bacterial-type flagellum-dependent swarming motility / bacterial-type flagellum / cytosol
Similarity search - Function
Flagellar hook protein FlgE superfamily / Flagellar hook protein FlgE / Flagellar basal body protein FlaE D2 domain / Flagellar hook-basal body protein, FlgE/F/G / Flagellar hook-basal body protein, FlgE/F/G-like / : / Flagellar hook protein FlgE/F/G D1 domain / Flagellar basal body rod protein, conserved site / Flagella basal body rod proteins signature. / Flagellar basal body rod protein, N-terminal ...Flagellar hook protein FlgE superfamily / Flagellar hook protein FlgE / Flagellar basal body protein FlaE D2 domain / Flagellar hook-basal body protein, FlgE/F/G / Flagellar hook-basal body protein, FlgE/F/G-like / : / Flagellar hook protein FlgE/F/G D1 domain / Flagellar basal body rod protein, conserved site / Flagella basal body rod proteins signature. / Flagellar basal body rod protein, N-terminal / Flagellar basal-body/hook protein, C-terminal domain / Flagella basal body rod protein / Flagellar basal body rod FlgEFG protein C-terminal
Similarity search - Domain/homology
Flagellar hook protein FlgE
Similarity search - Component
Biological speciesSalmonella typhimurium (bacteria)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.18 Å
AuthorsChen, L.X. / Jiang, W.X. / Cheng, X.Q. / Dong, X. / Xing, Q.
Funding support China, 2items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)32371277 China
National Natural Science Foundation of China (NSFC)32301028 China
CitationJournal: To Be Published
Title: Structure of flagellar hook at 3.18 angstroms resolution,conformation 1.
Authors: Chen, L.X. / Jiang, W.X. / Cheng, X.Q. / Dong, X. / Xing, Q.
History
DepositionMar 11, 2025Deposition site: PDBJ / Processing site: PDBC
Revision 1.0Mar 18, 2026Provider: repository / Type: Initial release
Revision 1.0Mar 18, 2026Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release
Revision 1.0Mar 18, 2026Data content type: FSC / Data content type: FSC / Provider: repository / Type: Initial release
Revision 1.0Mar 18, 2026Data content type: Half map / Part number: 1 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0Mar 18, 2026Data content type: Half map / Part number: 2 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0Mar 18, 2026Data content type: Image / Data content type: Image / Provider: repository / Type: Initial release
Revision 1.0Mar 18, 2026Data content type: Primary map / Data content type: Primary map / Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
DA: Flagellar hook protein FlgE
DB: Flagellar hook protein FlgE
DC: Flagellar hook protein FlgE
DD: Flagellar hook protein FlgE
DE: Flagellar hook protein FlgE
DF: Flagellar hook protein FlgE
DG: Flagellar hook protein FlgE
DH: Flagellar hook protein FlgE
DI: Flagellar hook protein FlgE
DJ: Flagellar hook protein FlgE
DK: Flagellar hook protein FlgE
EA: Flagellar hook protein FlgE
EB: Flagellar hook protein FlgE
EC: Flagellar hook protein FlgE
ED: Flagellar hook protein FlgE
EE: Flagellar hook protein FlgE
EF: Flagellar hook protein FlgE
EG: Flagellar hook protein FlgE
EH: Flagellar hook protein FlgE
EI: Flagellar hook protein FlgE
EJ: Flagellar hook protein FlgE
EK: Flagellar hook protein FlgE
FA: Flagellar hook protein FlgE
FB: Flagellar hook protein FlgE
FC: Flagellar hook protein FlgE
FD: Flagellar hook protein FlgE
FE: Flagellar hook protein FlgE
FF: Flagellar hook protein FlgE
FG: Flagellar hook protein FlgE
FH: Flagellar hook protein FlgE
FI: Flagellar hook protein FlgE
FJ: Flagellar hook protein FlgE
FK: Flagellar hook protein FlgE
GA: Flagellar hook protein FlgE
GB: Flagellar hook protein FlgE
GC: Flagellar hook protein FlgE
GD: Flagellar hook protein FlgE
GE: Flagellar hook protein FlgE
GF: Flagellar hook protein FlgE
GG: Flagellar hook protein FlgE
GH: Flagellar hook protein FlgE
GI: Flagellar hook protein FlgE
GJ: Flagellar hook protein FlgE
GK: Flagellar hook protein FlgE


Theoretical massNumber of molelcules
Total (without water)1,852,48644
Polymers1,852,48644
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

#1: Protein ...
Flagellar hook protein FlgE


Mass: 42101.957 Da / Num. of mol.: 44 / Source method: isolated from a natural source
Source: (natural) Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (bacteria)
References: UniProt: P0A1J1
Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: FILAMENT / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: FlgE / Type: ORGANELLE OR CELLULAR COMPONENT / Entity ID: all / Source: NATURAL
Source (natural)Organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria)
Buffer solutionpH: 8
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company
MicroscopyModel: FEI TALOS ARCTICA
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: OTHER
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 1000 nm
Image recordingElectron dose: 30 e/Å2 / Film or detector model: GATAN K3 (6k x 4k)

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Processing

EM softwareName: PHENIX / Category: model refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.18 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 435796 / Symmetry type: POINT
RefinementCross valid method: NONE

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