PDB-9m74: Crystal structure of MBP-fused BIL1/BZR1 (21-104) in complex with...

ID or keywords:

Entry

Database: PDB / ID: 9m74

Title

Crystal structure of MBP-fused BIL1/BZR1 (21-104) in complex with double-stranded DNA contaning CACAGCTGTG

Components

DNA (5'-D(*TP*TP*CP*AP*CP*AP*GP*CP*TP*GP*TP*GP*AP*AP*A)-3')
Maltodextrin-binding protein,Protein BRASSINAZOLE-RESISTANT 1

Keywords

DNA BINDING PROTEIN / Plant-specific / DNA-biding protein / Transcription factor / BZR

Function / homology

Function and homology information

plant ovule development / seed development / brassinosteroid mediated signaling pathway / cell envelope / carbohydrate transmembrane transporter activity / maltose binding / maltose transport / maltodextrin transmembrane transport / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / periplasmic space ...
Similarity search - Function

Biological species

Arabidopsis thaliana (thale cress)
synthetic construct (others)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 2.23 Å

Authors

Shohei, N. / Masaru, T. / Takuya, M.

Funding support

Japan, 6items

Organization	Grant number	Country
Ministry of Education, Culture, Sports, Science and Technology (Japan)	19H04855	Japan
Ministry of Education, Culture, Sports, Science and Technology (Japan)	19H04855	Japan
Ministry of Education, Culture, Sports, Science and Technology (Japan)	JP19K23658	Japan
Ministry of Education, Culture, Sports, Science and Technology (Japan)	21H02114	Japan
Japan Agency for Medical Research and Development (AMED)	JP20am0101077	Japan
Japan Agency for Medical Research and Development (AMED)	JP24ama121010	Japan

Citation

Journal: Febs Lett. / Year: 2025
Title: Single cis-elements in brassinosteroid-induced upregulated genes are insufficient to recruit both redox states of the BIL1/BZR1 DNA-binding domain.
Authors: Nosaki, S. / Ohtsuka, M. / Nakano, T. / Tanokura, M. / Miyakawa, T.

History

Deposition	Mar 9, 2025	Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0	Sep 10, 2025	Provider: repository / Type: Initial release

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	9m74.cif.gz	853.4 KB	Display	PDBx/mmCIF format
PDB format	pdb9m74.ent.gz	622.5 KB	Display	PDB format
PDBx/mmJSON format	9m74.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Summary document	9m74_validation.pdf.gz	1.9 MB	Display	wwPDB validaton report
Full document	9m74_full_validation.pdf.gz	1.9 MB	Display
Data in XML	9m74_validation.xml.gz	74.5 KB	Display
Data in CIF	9m74_validation.cif.gz	96 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/m7/9m74 ftp://data.pdbj.org/pub/pdb/validation_reports/m7/9m74	HTTPS FTP

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Related structure data

Related structure data	9m73C C: citing same article (ref.)
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#67 - Jul 2005 TATA-Binding Protein similarity (1) #168 - Dec 2013 DNA Helicase similarity (1) #255 - Mar 2021 Cisplatin and DNA similarity (1) #91 - Jul 2007 Thymine Dimers similarity (1) #122 - Feb 2010 Enhanceosome similarity (1) #172 - Apr 2014 RecA and Rad51 similarity (1) #181 - Jan 2015 Cascade and CRISPR similarity (1) #253 - Jan 2021 Expressome similarity (1) #112 - Apr 2009 Oct and Sox Transcription Factors similarity (1) #135 - Mar 2011 Integrase similarity (1) #150 - Jun 2012 Sliding Clamps similarity (1) #180 - Dec 2014 TAL Effectors similarity (1) #227 - Nov 2018 Telomerase similarity (1) #48 - Dec 2003 Catabolite Activator Protein similarity (1) #84 - Dec 2006 Transposase similarity (1) #139 - Jul 2011 DNA Methyltransferase similarity (1) #155 - Nov 2012 Vitamin D Receptor similarity (1) #3 - Mar 2000 DNA Polymerase similarity (1) #40 - Apr 2003 RNA Polymerase similarity (1) #73 - Jan 2006 Topoisomerase similarity (1) #87 - Mar 2007 Zinc Fingers similarity (1) #119 - Nov 2009 Designed DNA Crystal similarity (1) #125 - May 2010 Parvoviruses similarity (1) #131 - Nov 2010 Inteins similarity (1) #160 - Apr 2013 Actinomycin similarity (1) #190 - Oct 2015 Two-component Systems similarity (1) #23 - Nov 2001 DNA similarity (1) #240 - Dec 2019 Hypoxia-Inducible Factors similarity (1) #257 - May 2021 Fetal Hemoglobin similarity (1) #258 - Jun 2021 Glucocorticoid Receptor and Dexamethasone similarity (1) #31 - Jul 2002 p53 Tumor Suppressor similarity (1) #33 - Sep 2002 HIV Reverse Transcriptase similarity (1) #39 - Mar 2003 lac Repressor similarity (1) #45 - Sep 2003 Estrogen Receptor similarity (1) #55 - Jul 2004 DNA Ligase similarity (1) #7 - Jul 2000 Nucleosome similarity (1) #8 - Aug 2000 Restriction Enzymes similarity (1) #92 - Aug 2007 Anabolic Steroids similarity (1) #241 - Jan 2020 Twenty Years of Molecules similarity (1) #205 - Jan 2017 Nuclear Pore Complex similarity (6) #221 - May 2018 Human Papillomavirus and Vaccines similarity (6) #90 - Jun 2007 Fatty Acid Synthase similarity (3) #243 - Mar 2020 Voltage-gated Sodium Channels similarity (5) #100 - Apr 2008 Adrenergic Receptors similarity (1) #138 - Jun 2011 Glucansucrase similarity (1) #208 - Apr 2017 Glucose Transporters similarity (1) #74 - Feb 2006 alpha-Amylase similarity (1)

PDB pages

PDBj /

wwPDB /

NCBI

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Deposited unit

C: Maltodextrin-binding protein,Protein BRASSINAZOLE-RESISTANT 1

D: Maltodextrin-binding protein,Protein BRASSINAZOLE-RESISTANT 1

G: DNA (5'-D(*TP*TP*CP*AP*CP*AP*GP*CP*TP*GP*TP*GP*AP*AP*A)-3')

H: DNA (5'-D(*TP*TP*CP*AP*CP*AP*GP*CP*TP*GP*TP*GP*AP*AP*A)-3')

A: Maltodextrin-binding protein,Protein BRASSINAZOLE-RESISTANT 1

B: Maltodextrin-binding protein,Protein BRASSINAZOLE-RESISTANT 1

E: DNA (5'-D(*TP*TP*CP*AP*CP*AP*GP*CP*TP*GP*TP*GP*AP*AP*A)-3')

F: DNA (5'-D(*TP*TP*CP*AP*CP*AP*GP*CP*TP*GP*TP*GP*AP*AP*A)-3')

hetero molecules

219 kDa, 8 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

C: Maltodextrin-binding protein,Protein BRASSINAZOLE-RESISTANT 1

D: Maltodextrin-binding protein,Protein BRASSINAZOLE-RESISTANT 1

G: DNA (5'-D(*TP*TP*CP*AP*CP*AP*GP*CP*TP*GP*TP*GP*AP*AP*A)-3')

H: DNA (5'-D(*TP*TP*CP*AP*CP*AP*GP*CP*TP*GP*TP*GP*AP*AP*A)-3')

hetero molecules

defined by author&software
Evidence: isothermal titration calorimetry
110 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

A: Maltodextrin-binding protein,Protein BRASSINAZOLE-RESISTANT 1

B: Maltodextrin-binding protein,Protein BRASSINAZOLE-RESISTANT 1

E: DNA (5'-D(*TP*TP*CP*AP*CP*AP*GP*CP*TP*GP*TP*GP*AP*AP*A)-3')

F: DNA (5'-D(*TP*TP*CP*AP*CP*AP*GP*CP*TP*GP*TP*GP*AP*AP*A)-3')

hetero molecules

defined by author&software
Evidence: isothermal titration calorimetry
110 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	102.840, 92.506, 111.736
Angle α, β, γ (deg.)	90.000, 100.167, 90.000
Int Tables number	4
Space group name H-M	P12₁1
Space group name Hall	P2yb
Symmetry operation	#1: x,y,z #2: -x,y+1/2,-z

#1: Protein

Maltodextrin-binding protein,Protein BRASSINAZOLE-RESISTANT 1 / Protein BIN2 SUBSTRATE 2

Mass: 49819.445 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.)

Arabidopsis thaliana (thale cress) / Gene: malE, JW3994, BZR1, BIS2, At1g75080, F9E10.7 / Production host:

Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A0A4P1LXE0, UniProt: Q8S307

#2: DNA chain

DNA (5'-D(*TP*TP*CP*AP*CP*AP*GP*CP*TP*GP*TP*GP*AP*AP*A)-3')

Mass: 4593.011 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) synthetic construct (others) / Production host: synthetic construct (others)

#3: Polysaccharide

alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose

Type: oligosaccharide, Oligosaccharide / Class: Nutrient / Mass: 342.297 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Details: oligosaccharide / References: alpha-maltose

#4: Chemical

ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL

Mass: 62.068 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C₂H₆O₂

#5: Water

ChemComp-HOH / water

Mass: 18.015 Da / Num. of mol.: 185 / Source method: isolated from a natural source / Formula: H₂O

Has ligand of interest

N

Has protein modification

N

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.4 Å³/Da / Density % sol: 48.83 %
Crystal grow	Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: 50 mM sodium cacodylate pH 6.5, 200 mM ammonium acetate and 10 mM calcium chloride and 10% (w/v) polyethylene glycol (PEG) 4000

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Data collection

Diffraction	Mean temperature: 95 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: Photon Factory / Beamline: AR-NE3A / Wavelength: 1 Å
Detector	Type: DECTRIS PILATUS 2M-F / Detector: PIXEL / Date: Apr 19, 2017
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 1 Å / Relative weight: 1
Reflection	Resolution: 2.23→47.27 Å / Num. obs: 100538 / % possible obs: 100 % / Redundancy: 6.9 % / B_iso Wilson estimate: 39.6 Å² / CC_1/2: 0.999 / R_pim(I) all: 0.045 / Net I/σ(I): 16.7
Reflection shell	Resolution: 2.23→2.27 Å / Redundancy: 6.8 % / Mean I/σ(I) obs: 2.1 / Num. unique obs: 4938 / CC_1/2: 0.705 / R_pim(I) all: 0.568 / % possible all: 97.8

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Processing

Software

Name	Version	Classification
PHENIX	1.21.2_5419	refinement
XDS		data reduction
Aimless		data scaling
PHASER		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT / Resolution: 2.23→43.58 Å / SU ML: 0.2781 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 27.2067
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2

	R_factor	Num. reflection	% reflection
R_free	0.2438	5003	4.98 %
R_work	0.2151	95449	-
obs	0.2165	100452	99.9 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso mean: 54.91 Å²

Refinement step

Cycle: LAST / Resolution: 2.23→43.58 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	13592	1220	104	185	15101

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.0054	15381
X-RAY DIFFRACTION	f_angle_d	0.6619	21122
X-RAY DIFFRACTION	f_chiral_restr	0.0444	2306
X-RAY DIFFRACTION	f_plane_restr	0.0035	2526
X-RAY DIFFRACTION	f_dihedral_angle_d	24.1276	5831

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.23-2.26	0.3213	177	0.3018	3155	X-RAY DIFFRACTION	99.97
2.26-2.28	0.3725	179	0.2942	3139	X-RAY DIFFRACTION	99.97
2.28-2.31	0.3138	176	0.2794	3149	X-RAY DIFFRACTION	100
2.31-2.34	0.2738	192	0.2749	3163	X-RAY DIFFRACTION	99.94
2.34-2.37	0.3214	143	0.2682	3169	X-RAY DIFFRACTION	99.97
2.37-2.4	0.2768	167	0.2729	3154	X-RAY DIFFRACTION	99.97
2.4-2.44	0.3331	146	0.2773	3197	X-RAY DIFFRACTION	99.97
2.44-2.47	0.3402	141	0.2813	3189	X-RAY DIFFRACTION	99.97
2.47-2.51	0.2935	175	0.2726	3202	X-RAY DIFFRACTION	100
2.51-2.55	0.3462	171	0.2728	3148	X-RAY DIFFRACTION	100
2.55-2.6	0.3365	154	0.269	3201	X-RAY DIFFRACTION	100
2.6-2.64	0.3017	129	0.2524	3217	X-RAY DIFFRACTION	100
2.64-2.69	0.3008	145	0.2466	3157	X-RAY DIFFRACTION	99.97
2.69-2.75	0.2704	164	0.2513	3173	X-RAY DIFFRACTION	99.97
2.75-2.81	0.3127	133	0.2611	3232	X-RAY DIFFRACTION	99.97
2.81-2.87	0.2956	210	0.2585	3127	X-RAY DIFFRACTION	99.97
2.87-2.95	0.3082	161	0.2703	3179	X-RAY DIFFRACTION	99.94
2.95-3.03	0.3055	187	0.2701	3171	X-RAY DIFFRACTION	99.97
3.03-3.12	0.3425	156	0.2688	3175	X-RAY DIFFRACTION	100
3.12-3.22	0.2599	167	0.2577	3204	X-RAY DIFFRACTION	99.94
3.22-3.33	0.2564	171	0.2306	3131	X-RAY DIFFRACTION	99.82
3.33-3.46	0.2413	172	0.2108	3209	X-RAY DIFFRACTION	99.91
3.46-3.62	0.2428	156	0.2096	3192	X-RAY DIFFRACTION	99.97
3.62-3.81	0.2471	180	0.1997	3166	X-RAY DIFFRACTION	99.97
3.81-4.05	0.2205	176	0.1815	3204	X-RAY DIFFRACTION	99.88
4.05-4.36	0.2022	168	0.1625	3163	X-RAY DIFFRACTION	99.97
4.36-4.8	0.1806	170	0.1555	3215	X-RAY DIFFRACTION	99.91
4.8-5.5	0.1749	186	0.1658	3200	X-RAY DIFFRACTION	99.97
5.5-6.92	0.1925	173	0.1869	3241	X-RAY DIFFRACTION	99.82
6.92-43.58	0.1745	178	0.1651	3227	X-RAY DIFFRACTION	98.41

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	5.15686444371	-0.796793777005	-2.23608854879	2.99159932987	0.517628333436	3.1301153783	0.167319645523	-0.287695630103	0.430926272298	0.00200822489131	0.0538728270389	-0.185951776029	-0.300353466562	0.304321435879	-0.175437855234	0.31536514197	-0.0698906066342	0.0257858079336	0.276909477382	0.0023343518836	0.252387031098	-2.6284971378	14.2512498251	11.4518679759
2	5.31723164302	-1.56487225209	0.468649469866	4.91173415786	-1.38375264662	4.43090318375	-0.00460026671135	-0.428796651294	-0.775438915606	0.44899279952	0.0770174301719	-0.171561347495	0.936982799737	-0.0332344628388	-0.0596870818372	0.523865956231	-0.0540092661982	-0.0494252174327	0.306165810353	0.0071973094863	0.445059259377	-6.36184406867	-36.8846062784	27.7305277976
3	2.48135617237	-0.371838601903	-0.120407562107	0.655591321679	-0.145099483847	0.749461381482	-0.286501207512	-0.339783262544	0.331207368576	0.491490692237	0.689561870972	-0.887481346454	-0.055462957601	0.838952271202	-0.461145814053	0.810495294402	0.213973815632	-0.213298599789	1.12436130403	-0.546883735551	0.921235287637	4.6835046259	13.5317488189	45.5862408669
4	1.06131518768	0.312924373009	-0.288407639652	3.01084482204	0.706221171507	2.32992084528	-0.179065918002	-0.201052310708	0.0873280912813	0.662429956132	0.527593789822	-0.296111026972	-0.271760047048	0.222072440174	-0.28056046503	0.557250250271	0.212889408821	-0.0312438212376	0.656097691403	-0.102394057819	0.34292097093	-14.7558384855	13.7859092974	45.1112327458
5	0.561736451221	0.0957065697859	-0.199918164302	0.18065817953	-0.209793043954	0.346395370781	-0.0518982444319	0.144917463569	-0.253727631063	-0.0920826740504	0.036679326491	0.238199007847	0.667395038667	-0.319116828177	0.04541601434	0.609948723293	-0.123424969396	-0.0359346636416	0.379262911441	-0.0282813310926	0.556990147156	-2.02738393863	-36.7762342683	25.563728759
6	0.103393170715	-0.0295681608808	0.0490698234572	0.159576892938	0.0110510104002	0.0498661892126	0.481688444114	-0.549888861846	0.0266944650938	0.513188305432	0.0332971842144	-0.0136679128298	-0.286166190751	-0.443166343123	-0.475077808014	0.908911699771	-0.112750982175	0.051539385749	0.775455802094	0.0412592889185	0.367631367424	-5.83003968968	-23.7176034788	28.610444104
7	0.366669816886	-0.0539989332926	0.252560106704	0.517603403967	-0.239789830415	0.35988961846	0.273899585126	-0.113410556257	0.0820316408461	0.240260359077	-0.0501540601636	-0.109846345446	-0.34333173095	-0.0268398222424	-0.0607066884821	0.652807863094	0.0197366509264	0.0434411878755	0.515986686012	-0.0141474375443	0.289803808985	-2.90946846536	-23.6893844337	24.7832599386
8	3.18619081392	0.215581264184	-1.00320737538	0.996860410014	-0.00839638783724	1.764887983	0.0800025126697	-0.429236434128	-0.0516899856566	0.0732653645588	-0.0909118991142	-0.0478004660115	-0.00259042353843	0.436832480082	-0.0375010281111	0.295995477525	0.0189146955043	-0.0171172166727	0.39220241421	0.0124510520046	0.134499225845	39.8875059734	-13.2953051066	43.8752687807
9	1.19233600317	0.497218999598	1.12224870867	0.297460322303	0.417960359956	1.05141731128	-0.0322637062871	0.199698252405	0.203005096687	-0.60975525704	0.323964160116	0.250439644861	-0.155327648761	-0.195739696584	-0.460390346287	0.662100898693	-0.248773699602	-0.00078207942719	0.686512678414	0.0217759319534	0.40729057842	49.13406492	8.43711885667	44.0084193279
10	0.739360725461	-0.349848404833	0.157037303115	0.733414829391	0.755866892604	1.25728891945	0.068025330697	-0.380055369306	0.0607740101769	0.0457337959165	-0.0810936761412	-0.18249538076	0.0320039959357	0.032683058222	0.0162999018584	0.646241114262	0.0253533780755	-0.15099678043	0.630560697698	-0.0112188707899	0.571120443229	44.9625096001	36.3968939241	25.3154732866
11	2.74282849919	-0.0956922850007	-0.317552642752	1.06220257423	-0.507189368299	2.06064239483	-0.0417550464547	0.0863511777635	0.0921268249722	-0.0527573445908	0.077996212642	0.0721109535149	-0.0528928275682	-0.284459911804	-0.0596898739379	0.261821791721	-0.0229208217356	-0.00789310964268	0.219994848736	-0.014544658495	0.160262981711	52.7768465432	-14.0806173271	11.068956917
12	0.252607424274	0.29225315971	-0.00315798003456	0.208874821225	-0.156460649741	0.170415305786	0.0695532191687	0.265840604793	-0.10986471244	0.15308423037	-0.125267712879	0.18201432908	-0.0451032656998	0.120105776796	0.124350361451	0.691819012687	0.0994226767151	-0.146018815849	0.648810923767	-0.0121771551267	0.622671178064	48.8177292488	36.725684328	28.6852289142
13	0.557732561335	-0.109235470472	0.770509243862	2.13060892168	3.36050852437	4.92939708477	0.443509790268	-0.196651079501	-0.431316007883	-0.101623144579	-0.100700865273	0.565355761977	0.669636144184	-0.423546066726	0.112671443894	0.685054618505	0.0895078216366	0.0105368557843	0.973277579035	0.0133510531668	0.362511751052	47.0685890117	23.5526068475	29.8491834792
14	0.285400405677	-0.0512283675052	0.642697775488	0.622888184822	-2.91683990868	7.85614293167	0.613998549603	-1.2253946098	-1.35022765372	1.3025163865	0.413601897339	-2.49808362194	0.566746839748	0.708391127045	0.026710700339	0.663958289111	-0.00772620136459	0.422255687614	0.801771125284	0.305774919664	-4.13328868295	45.4434997803	23.4935116827	25.5937811335

Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

ID	Refine TLS-ID	Selection details	Auth asym-ID	Label asym-ID	Auth seq-ID	Label seq-ID
1	1	chain 'C' and (resid -348 through 26 )	C	A	-348 - 26	1 - 375
2	2	chain 'C' and (resid 27 through 88 )	C	A	27 - 88	376 - 437
3	3	chain 'D' and (resid -347 through -230 )	D	D	-347 - -230	1 - 118
4	4	chain 'D' and (resid -229 through 26 )	D	D	-229 - 26	119 - 374
5	5	chain 'D' and (resid 27 through 88 )	D	D	27 - 88	375 - 436
6	6	chain 'G' and (resid -3 through 11 )	G	F	-3 - 11
7	7	chain 'H' and (resid -3 through 11 )	H	G	-3 - 11
8	8	chain 'A' and (resid -348 through 3 )	A	H	-348 - 3	1 - 352
9	9	chain 'A' and (resid 4 through 26 )	A	H	4 - 26	353 - 375
10	10	chain 'A' and (resid 27 through 88 )	A	H	27 - 88	376 - 437
11	11	chain 'B' and (resid -347 through 26 )	B	K	-347 - 26	1 - 374
12	12	chain 'B' and (resid 27 through 88 )	B	K	27 - 88	375 - 436
13	13	chain 'E' and (resid -3 through 11 )	E	N	-3 - 11
14	14	chain 'F' and (resid -3 through 11 )	F	O	-3 - 11

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