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Yorodumi- PDB-9ly8: Cryo-EM structure of carboxysomal midi-shell: T=9 shell under C1 ... -
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| Entry | Database: PDB / ID: 9ly8 | ||||||
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| Title | Cryo-EM structure of carboxysomal midi-shell: T=9 shell under C1 symmetry | ||||||
 Components |
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 Keywords | STRUCTURAL PROTEIN / Carboxysome / shell / Icosahedron | ||||||
| Function / homology |  Function and homology informationstructural constituent of carboxysome shell / carboxysome / carbon fixation / viral translational frameshifting Similarity search - Function Carboxysome assembly protein / Carboxysome shell peptide mid-region / Carboxysome shell vertex protein CsoS4A / Ethanolamine utilization protein EutN/carboxysome shell vertex protein CcmL / EutN/Ccml superfamily / Ethanolamine utilisation protein EutN/carboxysome / Bacterial microcompartment vertex (BMV) domain profile. / Bacterial microcompartments protein, conserved site / Bacterial microcompartment (BMC) domain signature. / CcmK/CsoS1, bacterial microcompartment domain ...Carboxysome assembly protein / Carboxysome shell peptide mid-region / Carboxysome shell vertex protein CsoS4A / Ethanolamine utilization protein EutN/carboxysome shell vertex protein CcmL / EutN/Ccml superfamily / Ethanolamine utilisation protein EutN/carboxysome / Bacterial microcompartment vertex (BMV) domain profile. / Bacterial microcompartments protein, conserved site / Bacterial microcompartment (BMC) domain signature. / CcmK/CsoS1, bacterial microcompartment domain / : / Bacterial microcompartment (BMC) domain profile. / BMC domain / Bacterial microcompartment domain / BMC / CcmK-like superfamily Similarity search - Domain/homology Carboxysome assembly protein CsoS2B / Carboxysome shell vertex protein CsoS4A / Major carboxysome shell protein CsoS1A Similarity search - Component | ||||||
| Biological species |  Halothiobacillus neapolitanus c2 (bacteria) | ||||||
| Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.77 Å | ||||||
 Authors | Li, J.X. / Li, T.P. / Wang, S.M. / Zhang, Y.Z. / Liu, L.N. / Wang, P. | ||||||
| Funding support |  China, 1items
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 Citation | Journal: Plant Physiol. / Year: 2025 Title: Symmetry-adjusted cryo-EM analysis unveils the detailed linker protein CsoS2 interactions within the alpha-carboxysome shell. Authors: Li, J. / Li, T. / Wang, S. / Zhang, Y.Z. / Liu, L.N. / Wang, P. | ||||||
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Structure visualization
| Structure viewer | Molecule: MolmilJmol/JSmol |
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Downloads & links
-Download
| PDBx/mmCIF format | 9ly8.cif.gz | 9.9 MB | Display | PDBx/mmCIF format |
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| PDB format | pdb9ly8.ent.gz | Display | PDB format | |
| PDBx/mmJSON format | 9ly8.json.gz | Tree view | PDBx/mmJSON format | |
| Others |  Other downloads |
-Validation report
| Summary document | 9ly8_validation.pdf.gz | 6.9 MB | Display | wwPDB validaton report |
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| Full document | 9ly8_full_validation.pdf.gz | 8.2 MB | Display | |
| Data in XML | 9ly8_validation.xml.gz | 1.3 MB | Display | |
| Data in CIF | 9ly8_validation.cif.gz | 2.1 MB | Display | |
| Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ly/9ly8ftp://data.pdbj.org/pub/pdb/validation_reports/ly/9ly8 | HTTPS FTP |
-Related structure data
| Related structure data |  9ly9C M: map data used to model this data C: citing same article ( |
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| Similar structure data |
-Links
PDBj
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Assembly
| Deposited unit | A2: Major carboxysome shell protein CsoS1A B2: Major carboxysome shell protein CsoS1A C2: Major carboxysome shell protein CsoS1A D2: Major carboxysome shell protein CsoS1A E2: Major carboxysome shell protein CsoS1A F2: Major carboxysome shell protein CsoS1A G2: Major carboxysome shell protein CsoS1A H2: Major carboxysome shell protein CsoS1A X2: Carboxysome assembly protein CsoS2B P2: Carboxysome shell vertex protein CsoS4A A1: Major carboxysome shell protein CsoS1A B1: Major carboxysome shell protein CsoS1A C1: Major carboxysome shell protein CsoS1A D1: Major carboxysome shell protein CsoS1A E1: Major carboxysome shell protein CsoS1A F1: Major carboxysome shell protein CsoS1A G1: Major carboxysome shell protein CsoS1A X1: Carboxysome assembly protein CsoS2B P1: Carboxysome shell vertex protein CsoS4A A5: Major carboxysome shell protein CsoS1A B5: Major carboxysome shell protein CsoS1A C5: Major carboxysome shell protein CsoS1A D5: Major carboxysome shell protein CsoS1A E5: Major carboxysome shell protein CsoS1A F5: Major carboxysome shell protein CsoS1A G5: Major carboxysome shell protein CsoS1A H5: Major carboxysome shell protein CsoS1A X5: Carboxysome assembly protein CsoS2B P5: Carboxysome shell vertex protein CsoS4A A3: Major carboxysome shell protein CsoS1A B3: Major carboxysome shell protein CsoS1A C3: Major carboxysome shell protein CsoS1A D3: Major carboxysome shell protein CsoS1A E3: Major carboxysome shell protein CsoS1A F3: Major carboxysome shell protein CsoS1A G3: Major carboxysome shell protein CsoS1A H3: Major carboxysome shell protein CsoS1A X3: Carboxysome assembly protein CsoS2B P3: Carboxysome shell vertex protein CsoS4A A4: Major carboxysome shell protein CsoS1A B4: Major carboxysome shell protein CsoS1A C4: Major carboxysome shell protein CsoS1A D4: Major carboxysome shell protein CsoS1A E4: Major carboxysome shell protein CsoS1A F4: Major carboxysome shell protein CsoS1A G4: Major carboxysome shell protein CsoS1A H4: Major carboxysome shell protein CsoS1A X4: Carboxysome assembly protein CsoS2B P4: Carboxysome shell vertex protein CsoS4A A7: Major carboxysome shell protein CsoS1A B7: Major carboxysome shell protein CsoS1A C7: Major carboxysome shell protein CsoS1A D7: Major carboxysome shell protein CsoS1A E7: Major carboxysome shell protein CsoS1A F7: Major carboxysome shell protein CsoS1A G7: Major carboxysome shell protein CsoS1A H7: Major carboxysome shell protein CsoS1A X7: Carboxysome assembly protein CsoS2B P7: Carboxysome shell vertex protein CsoS4A A6: Major carboxysome shell protein CsoS1A B6: Major carboxysome shell protein CsoS1A C6: Major carboxysome shell protein CsoS1A D6: Major carboxysome shell protein CsoS1A E6: Major carboxysome shell protein CsoS1A F6: Major carboxysome shell protein CsoS1A G6: Major carboxysome shell protein CsoS1A X6: Carboxysome assembly protein CsoS2B P6: Carboxysome shell vertex protein CsoS4A A0: Major carboxysome shell protein CsoS1A B0: Major carboxysome shell protein CsoS1A C0: Major carboxysome shell protein CsoS1A D0: Major carboxysome shell protein CsoS1A E0: Major carboxysome shell protein CsoS1A F0: Major carboxysome shell protein CsoS1A G0: Major carboxysome shell protein CsoS1A H0: Major carboxysome shell protein CsoS1A X0: Carboxysome assembly protein CsoS2B P0: Carboxysome shell vertex protein CsoS4A A8: Major carboxysome shell protein CsoS1A B8: Major carboxysome shell protein CsoS1A C8: Major carboxysome shell protein CsoS1A D8: Major carboxysome shell protein CsoS1A E8: Major carboxysome shell protein CsoS1A F8: Major carboxysome shell protein CsoS1A G8: Major carboxysome shell protein CsoS1A H8: Major carboxysome shell protein CsoS1A P8: Carboxysome shell vertex protein CsoS4A A9: Major carboxysome shell protein CsoS1A B9: Major carboxysome shell protein CsoS1A C9: Major carboxysome shell protein CsoS1A D9: Major carboxysome shell protein CsoS1A E9: Major carboxysome shell protein CsoS1A F9: Major carboxysome shell protein CsoS1A G9: Major carboxysome shell protein CsoS1A H9: Major carboxysome shell protein CsoS1A X9: Carboxysome assembly protein CsoS2B P9: Carboxysome shell vertex protein CsoS4A Ab: Major carboxysome shell protein CsoS1A Bb: Major carboxysome shell protein CsoS1A Cb: Major carboxysome shell protein CsoS1A Db: Major carboxysome shell protein CsoS1A Eb: Major carboxysome shell protein CsoS1A Fb: Major carboxysome shell protein CsoS1A Gb: Major carboxysome shell protein CsoS1A Hb: Major carboxysome shell protein CsoS1A Xb: Carboxysome assembly protein CsoS2B Pb: Carboxysome shell vertex protein CsoS4A Aa: Major carboxysome shell protein CsoS1A Ba: Major carboxysome shell protein CsoS1A Ca: Major carboxysome shell protein CsoS1A Da: Major carboxysome shell protein CsoS1A Ea: Major carboxysome shell protein CsoS1A Fa: Major carboxysome shell protein CsoS1A Ga: Major carboxysome shell protein CsoS1A Ha: Major carboxysome shell protein CsoS1A Xa: Carboxysome assembly protein CsoS2B Pa: Carboxysome shell vertex protein CsoS4A Ae: Major carboxysome shell protein CsoS1A Be: Major carboxysome shell protein CsoS1A Ce: Major carboxysome shell protein CsoS1A De: Major carboxysome shell protein CsoS1A Ee: Major carboxysome shell protein CsoS1A Fe: Major carboxysome shell protein CsoS1A Ge: Major carboxysome shell protein CsoS1A He: Major carboxysome shell protein CsoS1A Xe: Carboxysome assembly protein CsoS2B Pe: Carboxysome shell vertex protein CsoS4A Ac: Major carboxysome shell protein CsoS1A Bc: Major carboxysome shell protein CsoS1A Cc: Major carboxysome shell protein CsoS1A Dc: Major carboxysome shell protein CsoS1A Ec: Major carboxysome shell protein CsoS1A Fc: Major carboxysome shell protein CsoS1A Gc: Major carboxysome shell protein CsoS1A Hc: Major carboxysome shell protein CsoS1A Pc: Carboxysome shell vertex protein CsoS4A Ad: Major carboxysome shell protein CsoS1A Bd: Major carboxysome shell protein CsoS1A Cd: Major carboxysome shell protein CsoS1A Dd: Major carboxysome shell protein CsoS1A Ed: Major carboxysome shell protein CsoS1A Fd: Major carboxysome shell protein CsoS1A Gd: Major carboxysome shell protein CsoS1A Hd: Major carboxysome shell protein CsoS1A Xd: Carboxysome assembly protein CsoS2B Pd: Carboxysome shell vertex protein CsoS4A Ag: Major carboxysome shell protein CsoS1A Bg: Major carboxysome shell protein CsoS1A Cg: Major carboxysome shell protein CsoS1A Dg: Major carboxysome shell protein CsoS1A Eg: Major carboxysome shell protein CsoS1A Fg: Major carboxysome shell protein CsoS1A Gg: Major carboxysome shell protein CsoS1A Hg: Major carboxysome shell protein CsoS1A Xg: Carboxysome assembly protein CsoS2B Pg: Carboxysome shell vertex protein CsoS4A Af: Major carboxysome shell protein CsoS1A Bf: Major carboxysome shell protein CsoS1A Cf: Major carboxysome shell protein CsoS1A Df: Major carboxysome shell protein CsoS1A Ef: Major carboxysome shell protein CsoS1A Ff: Major carboxysome shell protein CsoS1A Gf: Major carboxysome shell protein CsoS1A Xf: Carboxysome assembly protein CsoS2B Pf: Carboxysome shell vertex protein CsoS4A Aj: Major carboxysome shell protein CsoS1A Bj: Major carboxysome shell protein CsoS1A Cj: Major carboxysome shell protein CsoS1A Dj: Major carboxysome shell protein CsoS1A Ej: Major carboxysome shell protein CsoS1A Fj: Major carboxysome shell protein CsoS1A Gj: Major carboxysome shell protein CsoS1A Hj: Major carboxysome shell protein CsoS1A Xj: Carboxysome assembly protein CsoS2B Pj: Carboxysome shell vertex protein CsoS4A Ah: Major carboxysome shell protein CsoS1A Bh: Major carboxysome shell protein CsoS1A Ch: Major carboxysome shell protein CsoS1A Dh: Major carboxysome shell protein CsoS1A Eh: Major carboxysome shell protein CsoS1A Fh: Major carboxysome shell protein CsoS1A Gh: Major carboxysome shell protein CsoS1A Hh: Major carboxysome shell protein CsoS1A Xh: Carboxysome assembly protein CsoS2B Ph: Carboxysome shell vertex protein CsoS4A Ai: Major carboxysome shell protein CsoS1A Bi: Major carboxysome shell protein CsoS1A Ci: Major carboxysome shell protein CsoS1A Di: Major carboxysome shell protein CsoS1A Ei: Major carboxysome shell protein CsoS1A Fi: Major carboxysome shell protein CsoS1A Gi: Major carboxysome shell protein CsoS1A Hi: Major carboxysome shell protein CsoS1A Xi: Carboxysome assembly protein CsoS2B Pi: Carboxysome shell vertex protein CsoS4A Al: Major carboxysome shell protein CsoS1A Bl: Major carboxysome shell protein CsoS1A Cl: Major carboxysome shell protein CsoS1A Dl: Major carboxysome shell protein CsoS1A El: Major carboxysome shell protein CsoS1A Fl: Major carboxysome shell protein CsoS1A Gl: Major carboxysome shell protein CsoS1A Hl: Major carboxysome shell protein CsoS1A Xl: Carboxysome assembly protein CsoS2B Pl: Carboxysome shell vertex protein CsoS4A Ak: Major carboxysome shell protein CsoS1A Bk: Major carboxysome shell protein CsoS1A Ck: Major carboxysome shell protein CsoS1A Dk: Major carboxysome shell protein CsoS1A Ek: Major carboxysome shell protein CsoS1A Fk: Major carboxysome shell protein CsoS1A Gk: Major carboxysome shell protein CsoS1A Xk: Carboxysome assembly protein CsoS2B Pk: Carboxysome shell vertex protein CsoS4A Ao: Major carboxysome shell protein CsoS1A Bo: Major carboxysome shell protein CsoS1A Co: Major carboxysome shell protein CsoS1A Do: Major carboxysome shell protein CsoS1A Eo: Major carboxysome shell protein CsoS1A Fo: Major carboxysome shell protein CsoS1A Go: Major carboxysome shell protein CsoS1A Ho: Major carboxysome shell protein CsoS1A Xo: Carboxysome assembly protein CsoS2B Po: Carboxysome shell vertex protein CsoS4A Am: Major carboxysome shell protein CsoS1A Bm: Major carboxysome shell protein CsoS1A Cm: Major carboxysome shell protein CsoS1A Dm: Major carboxysome shell protein CsoS1A Em: Major carboxysome shell protein CsoS1A Fm: Major carboxysome shell protein CsoS1A Gm: Major carboxysome shell protein CsoS1A Hm: Major carboxysome shell protein CsoS1A Pm: Carboxysome shell vertex protein CsoS4A An: Major carboxysome shell protein CsoS1A Bn: Major carboxysome shell protein CsoS1A Cn: Major carboxysome shell protein CsoS1A Dn: Major carboxysome shell protein CsoS1A En: Major carboxysome shell protein CsoS1A Fn: Major carboxysome shell protein CsoS1A Gn: Major carboxysome shell protein CsoS1A Hn: Major carboxysome shell protein CsoS1A Xn: Carboxysome assembly protein CsoS2B Pn: Carboxysome shell vertex protein CsoS4A Aq: Major carboxysome shell protein CsoS1A Bq: Major carboxysome shell protein CsoS1A Cq: Major carboxysome shell protein CsoS1A Dq: Major carboxysome shell protein CsoS1A Eq: Major carboxysome shell protein CsoS1A Fq: Major carboxysome shell protein CsoS1A Gq: Major carboxysome shell protein CsoS1A Hq: Major carboxysome shell protein CsoS1A Xq: Carboxysome assembly protein CsoS2B Pq: Carboxysome shell vertex protein CsoS4A Ap: Major carboxysome shell protein CsoS1A Bp: Major carboxysome shell protein CsoS1A Cp: Major carboxysome shell protein CsoS1A Dp: Major carboxysome shell protein CsoS1A Ep: Major carboxysome shell protein CsoS1A Fp: Major carboxysome shell protein CsoS1A Gp: Major carboxysome shell protein CsoS1A Hp: Major carboxysome shell protein CsoS1A Xp: Carboxysome assembly protein CsoS2B Pp: Carboxysome shell vertex protein CsoS4A At: Major carboxysome shell protein CsoS1A Bt: Major carboxysome shell protein CsoS1A Ct: Major carboxysome shell protein CsoS1A Dt: Major carboxysome shell protein CsoS1A Et: Major carboxysome shell protein CsoS1A Ft: Major carboxysome shell protein CsoS1A Gt: Major carboxysome shell protein CsoS1A Ht: Major carboxysome shell protein CsoS1A Xt: Carboxysome assembly protein CsoS2B Pt: Carboxysome shell vertex protein CsoS4A Ar: Major carboxysome shell protein CsoS1A Br: Major carboxysome shell protein CsoS1A Cr: Major carboxysome shell protein CsoS1A Dr: Major carboxysome shell protein CsoS1A Er: Major carboxysome shell protein CsoS1A Fr: Major carboxysome shell protein CsoS1A Gr: Major carboxysome shell protein CsoS1A Hr: Major carboxysome shell protein CsoS1A Pr: Carboxysome shell vertex protein CsoS4A As: Major carboxysome shell protein CsoS1A Bs: Major carboxysome shell protein CsoS1A Cs: Major carboxysome shell protein CsoS1A Ds: Major carboxysome shell protein CsoS1A Es: Major carboxysome shell protein CsoS1A Fs: Major carboxysome shell protein CsoS1A Gs: Major carboxysome shell protein CsoS1A Hs: Major carboxysome shell protein CsoS1A Xs: Carboxysome assembly protein CsoS2B Ps: Carboxysome shell vertex protein CsoS4A Av: Major carboxysome shell protein CsoS1A Bv: Major carboxysome shell protein CsoS1A Cv: Major carboxysome shell protein CsoS1A Dv: Major carboxysome shell protein CsoS1A Ev: Major carboxysome shell protein CsoS1A Fv: Major carboxysome shell protein CsoS1A Gv: Major carboxysome shell protein CsoS1A Hv: Major carboxysome shell protein CsoS1A Xv: Carboxysome assembly protein CsoS2B Pv: Carboxysome shell vertex protein CsoS4A Au: Major carboxysome shell protein CsoS1A Bu: Major carboxysome shell protein CsoS1A Cu: Major carboxysome shell protein CsoS1A Du: Major carboxysome shell protein CsoS1A Eu: Major carboxysome shell protein CsoS1A Fu: Major carboxysome shell protein CsoS1A Gu: Major carboxysome shell protein CsoS1A Xu: Carboxysome assembly protein CsoS2B Pu: Carboxysome shell vertex protein CsoS4A Ay: Major carboxysome shell protein CsoS1A By: Major carboxysome shell protein CsoS1A Cy: Major carboxysome shell protein CsoS1A Dy: Major carboxysome shell protein CsoS1A Ey: Major carboxysome shell protein CsoS1A Fy: Major carboxysome shell protein CsoS1A Gy: Major carboxysome shell protein CsoS1A Hy: Major carboxysome shell protein CsoS1A Xy: Carboxysome assembly protein CsoS2B Py: Carboxysome shell vertex protein CsoS4A Aw: Major carboxysome shell protein CsoS1A Bw: Major carboxysome shell protein CsoS1A Cw: Major carboxysome shell protein CsoS1A Dw: Major carboxysome shell protein CsoS1A Ew: Major carboxysome shell protein CsoS1A Fw: Major carboxysome shell protein CsoS1A Gw: Major carboxysome shell protein CsoS1A Hw: Major carboxysome shell protein CsoS1A Xw: Carboxysome assembly protein CsoS2B Pw: Carboxysome shell vertex protein CsoS4A Ax: Major carboxysome shell protein CsoS1A Bx: Major carboxysome shell protein CsoS1A Cx: Major carboxysome shell protein CsoS1A Dx: Major carboxysome shell protein CsoS1A Ex: Major carboxysome shell protein CsoS1A Fx: Major carboxysome shell protein CsoS1A Gx: Major carboxysome shell protein CsoS1A Hx: Major carboxysome shell protein CsoS1A Xx: Carboxysome assembly protein CsoS2B Px: Carboxysome shell vertex protein CsoS4A AA: Major carboxysome shell protein CsoS1A BA: Major carboxysome shell protein CsoS1A CA: Major carboxysome shell protein CsoS1A DA: Major carboxysome shell protein CsoS1A EA: Major carboxysome shell protein CsoS1A FA: Major carboxysome shell protein CsoS1A GA: Major carboxysome shell protein CsoS1A HA: Major carboxysome shell protein CsoS1A XA: Carboxysome assembly protein CsoS2B PA: Carboxysome shell vertex protein CsoS4A Az: Major carboxysome shell protein CsoS1A Bz: Major carboxysome shell protein CsoS1A Cz: Major carboxysome shell protein CsoS1A Dz: Major carboxysome shell protein CsoS1A Ez: Major carboxysome shell protein CsoS1A Fz: Major carboxysome shell protein CsoS1A Gz: Major carboxysome shell protein CsoS1A Xz: Carboxysome assembly protein CsoS2B Pz: Carboxysome shell vertex protein CsoS4A AD: Major carboxysome shell protein CsoS1A BD: Major carboxysome shell protein CsoS1A CD: Major carboxysome shell protein CsoS1A DD: Major carboxysome shell protein CsoS1A ED: Major carboxysome shell protein CsoS1A FD: Major carboxysome shell protein CsoS1A GD: Major carboxysome shell protein CsoS1A HD: Major carboxysome shell protein CsoS1A XD: Carboxysome assembly protein CsoS2B PD: Carboxysome shell vertex protein CsoS4A AB: Major carboxysome shell protein CsoS1A BB: Major carboxysome shell protein CsoS1A CB: Major carboxysome shell protein CsoS1A DB: Major carboxysome shell protein CsoS1A EB: Major carboxysome shell protein CsoS1A FB: Major carboxysome shell protein CsoS1A GB: Major carboxysome shell protein CsoS1A HB: Major carboxysome shell protein CsoS1A PB: Carboxysome shell vertex protein CsoS4A AC: Major carboxysome shell protein CsoS1A BC: Major carboxysome shell protein CsoS1A CC: Major carboxysome shell protein CsoS1A DC: Major carboxysome shell protein CsoS1A EC: Major carboxysome shell protein CsoS1A FC: Major carboxysome shell protein CsoS1A GC: Major carboxysome shell protein CsoS1A HC: Major carboxysome shell protein CsoS1A XC: Carboxysome assembly protein CsoS2B PC: Carboxysome shell vertex protein CsoS4A AF: Major carboxysome shell protein CsoS1A BF: Major carboxysome shell protein CsoS1A CF: Major carboxysome shell protein CsoS1A DF: Major carboxysome shell protein CsoS1A EF: Major carboxysome shell protein CsoS1A FF: Major carboxysome shell protein CsoS1A GF: Major carboxysome shell protein CsoS1A HF: Major carboxysome shell protein CsoS1A XF: Carboxysome assembly protein CsoS2B PF: Carboxysome shell vertex protein CsoS4A AE: Major carboxysome shell protein CsoS1A BE: Major carboxysome shell protein CsoS1A CE: Major carboxysome shell protein CsoS1A DE: Major carboxysome shell protein CsoS1A EE: Major carboxysome shell protein CsoS1A FE: Major carboxysome shell protein CsoS1A GE: Major carboxysome shell protein CsoS1A HE: Major carboxysome shell protein CsoS1A XE: Carboxysome assembly protein CsoS2B PE: Carboxysome shell vertex protein CsoS4A AI: Major carboxysome shell protein CsoS1A BI: Major carboxysome shell protein CsoS1A CI: Major carboxysome shell protein CsoS1A DI: Major carboxysome shell protein CsoS1A EI: Major carboxysome shell protein CsoS1A FI: Major carboxysome shell protein CsoS1A GI: Major carboxysome shell protein CsoS1A HI: Major carboxysome shell protein CsoS1A XI: Carboxysome assembly protein CsoS2B PI: Carboxysome shell vertex protein CsoS4A AG: Major carboxysome shell protein CsoS1A BG: Major carboxysome shell protein CsoS1A CG: Major carboxysome shell protein CsoS1A DG: Major carboxysome shell protein CsoS1A EG: Major carboxysome shell protein CsoS1A FG: Major carboxysome shell protein CsoS1A GG: Major carboxysome shell protein CsoS1A HG: Major carboxysome shell protein CsoS1A PG: Carboxysome shell vertex protein CsoS4A AH: Major carboxysome shell protein CsoS1A BH: Major carboxysome shell protein CsoS1A CH: Major carboxysome shell protein CsoS1A DH: Major carboxysome shell protein CsoS1A EH: Major carboxysome shell protein CsoS1A FH: Major carboxysome shell protein CsoS1A GH: Major carboxysome shell protein CsoS1A HH: Major carboxysome shell protein CsoS1A XH: Carboxysome assembly protein CsoS2B PH: Carboxysome shell vertex protein CsoS4A AK: Major carboxysome shell protein CsoS1A BK: Major carboxysome shell protein CsoS1A CK: Major carboxysome shell protein CsoS1A DK: Major carboxysome shell protein CsoS1A EK: Major carboxysome shell protein CsoS1A FK: Major carboxysome shell protein CsoS1A GK: Major carboxysome shell protein CsoS1A HK: Major carboxysome shell protein CsoS1A XK: Carboxysome assembly protein CsoS2B PK: Carboxysome shell vertex protein CsoS4A AJ: Major carboxysome shell protein CsoS1A BJ: Major carboxysome shell protein CsoS1A CJ: Major carboxysome shell protein CsoS1A DJ: Major carboxysome shell protein CsoS1A EJ: Major carboxysome shell protein CsoS1A FJ: Major carboxysome shell protein CsoS1A GJ: Major carboxysome shell protein CsoS1A HJ: Major carboxysome shell protein CsoS1A XJ: Carboxysome assembly protein CsoS2B PJ: Carboxysome shell vertex protein CsoS4A AN: Major carboxysome shell protein CsoS1A BN: Major carboxysome shell protein CsoS1A CN: Major carboxysome shell protein CsoS1A DN: Major carboxysome shell protein CsoS1A EN: Major carboxysome shell protein CsoS1A FN: Major carboxysome shell protein CsoS1A GN: Major carboxysome shell protein CsoS1A HN: Major carboxysome shell protein CsoS1A XN: Carboxysome assembly protein CsoS2B PN: Carboxysome shell vertex protein CsoS4A AL: Major carboxysome shell protein CsoS1A BL: Major carboxysome shell protein CsoS1A CL: Major carboxysome shell protein CsoS1A DL: Major carboxysome shell protein CsoS1A EL: Major carboxysome shell protein CsoS1A FL: Major carboxysome shell protein CsoS1A GL: Major carboxysome shell protein CsoS1A HL: Major carboxysome shell protein CsoS1A XL: Carboxysome assembly protein CsoS2B PL: Carboxysome shell vertex protein CsoS4A AM: Major carboxysome shell protein CsoS1A BM: Major carboxysome shell protein CsoS1A CM: Major carboxysome shell protein CsoS1A DM: Major carboxysome shell protein CsoS1A EM: Major carboxysome shell protein CsoS1A FM: Major carboxysome shell protein CsoS1A GM: Major carboxysome shell protein CsoS1A HM: Major carboxysome shell protein CsoS1A XM: Carboxysome assembly protein CsoS2B PM: Carboxysome shell vertex protein CsoS4A AP: Major carboxysome shell protein CsoS1A BP: Major carboxysome shell protein CsoS1A CP: Major carboxysome shell protein CsoS1A DP: Major carboxysome shell protein CsoS1A EP: Major carboxysome shell protein CsoS1A FP: Major carboxysome shell protein CsoS1A GP: Major carboxysome shell protein CsoS1A HP: Major carboxysome shell protein CsoS1A XP: Carboxysome assembly protein CsoS2B PP: Carboxysome shell vertex protein CsoS4A AO: Major carboxysome shell protein CsoS1A BO: Major carboxysome shell protein CsoS1A CO: Major carboxysome shell protein CsoS1A DO: Major carboxysome shell protein CsoS1A EO: Major carboxysome shell protein CsoS1A FO: Major carboxysome shell protein CsoS1A GO: Major carboxysome shell protein CsoS1A XO: Carboxysome assembly protein CsoS2B PO: Carboxysome shell vertex protein CsoS4A AS: Major carboxysome shell protein CsoS1A BS: Major carboxysome shell protein CsoS1A CS: Major carboxysome shell protein CsoS1A DS: Major carboxysome shell protein CsoS1A ES: Major carboxysome shell protein CsoS1A FS: Major carboxysome shell protein CsoS1A GS: Major carboxysome shell protein CsoS1A HS: Major carboxysome shell protein CsoS1A XS: Carboxysome assembly protein CsoS2B PS: Carboxysome shell vertex protein CsoS4A AQ: Major carboxysome shell protein CsoS1A BQ: Major carboxysome shell protein CsoS1A CQ: Major carboxysome shell protein CsoS1A DQ: Major carboxysome shell protein CsoS1A EQ: Major carboxysome shell protein CsoS1A FQ: Major carboxysome shell protein CsoS1A GQ: Major carboxysome shell protein CsoS1A HQ: Major carboxysome shell protein CsoS1A XQ: Carboxysome assembly protein CsoS2B PQ: Carboxysome shell vertex protein CsoS4A AR: Major carboxysome shell protein CsoS1A BR: Major carboxysome shell protein CsoS1A CR: Major carboxysome shell protein CsoS1A DR: Major carboxysome shell protein CsoS1A ER: Major carboxysome shell protein CsoS1A FR: Major carboxysome shell protein CsoS1A GR: Major carboxysome shell protein CsoS1A HR: Major carboxysome shell protein CsoS1A XR: Carboxysome assembly protein CsoS2B PR: Carboxysome shell vertex protein CsoS4A AU: Major carboxysome shell protein CsoS1A BU: Major carboxysome shell protein CsoS1A CU: Major carboxysome shell protein CsoS1A DU: Major carboxysome shell protein CsoS1A EU: Major carboxysome shell protein CsoS1A FU: Major carboxysome shell protein CsoS1A GU: Major carboxysome shell protein CsoS1A HU: Major carboxysome shell protein CsoS1A XU: Carboxysome assembly protein CsoS2B PU: Carboxysome shell vertex protein CsoS4A AT: Major carboxysome shell protein CsoS1A BT: Major carboxysome shell protein CsoS1A CT: Major carboxysome shell protein CsoS1A DT: Major carboxysome shell protein CsoS1A ET: Major carboxysome shell protein CsoS1A FT: Major carboxysome shell protein CsoS1A GT: Major carboxysome shell protein CsoS1A HT: Major carboxysome shell protein CsoS1A XT: Carboxysome assembly protein CsoS2B PT: Carboxysome shell vertex protein CsoS4A AX: Major carboxysome shell protein CsoS1A BX: Major carboxysome shell protein CsoS1A CX: Major carboxysome shell protein CsoS1A DX: Major carboxysome shell protein CsoS1A EX: Major carboxysome shell protein CsoS1A FX: Major carboxysome shell protein CsoS1A GX: Major carboxysome shell protein CsoS1A HX: Major carboxysome shell protein CsoS1A XX: Carboxysome assembly protein CsoS2B PX: Carboxysome shell vertex protein CsoS4A AV: Major carboxysome shell protein CsoS1A BV: Major carboxysome shell protein CsoS1A CV: Major carboxysome shell protein CsoS1A DV: Major carboxysome shell protein CsoS1A EV: Major carboxysome shell protein CsoS1A FV: Major carboxysome shell protein CsoS1A GV: Major carboxysome shell protein CsoS1A HV: Major carboxysome shell protein CsoS1A PV: Carboxysome shell vertex protein CsoS4A AW: Major carboxysome shell protein CsoS1A BW: Major carboxysome shell protein CsoS1A CW: Major carboxysome shell protein CsoS1A DW: Major carboxysome shell protein CsoS1A EW: Major carboxysome shell protein CsoS1A FW: Major carboxysome shell protein CsoS1A GW: Major carboxysome shell protein CsoS1A HW: Major carboxysome shell protein CsoS1A XW: Carboxysome assembly protein CsoS2B PW: Carboxysome shell vertex protein CsoS4A H1: Major carboxysome shell protein CsoS1A H6: Major carboxysome shell protein CsoS1A X8: Carboxysome assembly protein CsoS2B Xc: Carboxysome assembly protein CsoS2B Hf: Major carboxysome shell protein CsoS1A Hk: Major carboxysome shell protein CsoS1A Xm: Carboxysome assembly protein CsoS2B Xr: Carboxysome assembly protein CsoS2B Hu: Major carboxysome shell protein CsoS1A Hz: Major carboxysome shell protein CsoS1A XB: Carboxysome assembly protein CsoS2B XG: Carboxysome assembly protein CsoS2B HO: Major carboxysome shell protein CsoS1A XV: Carboxysome assembly protein CsoS2B 
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
-Components
| #1: Protein | Mass: 9973.478 Da / Num. of mol.: 480 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Halothiobacillus neapolitanus c2 (bacteria)Gene: csoS1A, csoS1, Hneap_0915 / Production host:  Escherichia coli (E. coli) / References: UniProt: P45689#2: Protein | Mass: 92048.617 Da / Num. of mol.: 60 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Halothiobacillus neapolitanus c2 (bacteria)Gene: csoS2, Hneap_0920 / Production host: Escherichia coli (E. coli) / References: UniProt: O85041#3: Protein | Mass: 8900.287 Da / Num. of mol.: 60 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Halothiobacillus neapolitanus c2 (bacteria)Gene: csoS4A, orfA, Hneap_0918 / Production host: Escherichia coli (E. coli) / References: UniProt: O85043Has protein modification | Y | |
|---|
-Experimental details
-Experiment
| Experiment | Method: ELECTRON MICROSCOPY |
|---|---|
| EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-
Sample preparation
| Component | Name: Cryo-EM structure of carboxysomal midi-shell: T=9 shell under C1 symmetry Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
|---|---|
| Source (natural) | Organism: Halothiobacillus neapolitanus (bacteria) |
| Source (recombinant) | Organism: Escherichia coli (E. coli) |
| Buffer solution | pH: 8 |
| Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
| Vitrification | Cryogen name: ETHANE |
-
Electron microscopy imaging
| Experimental equipment |  Model: Titan Krios / Image courtesy: FEI Company |
|---|---|
| Microscopy | Model: TFS KRIOS |
| Electron gun | Electron source:  FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: SPOT SCAN |
| Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2500 nm / Nominal defocus min: 1200 nm |
| Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-
Processing
| CTF correction | Type: NONE |
|---|---|
| 3D reconstruction | Resolution: 2.77 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 40177 / Symmetry type: POINT |
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