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Yorodumi- PDB-9le8: Coordinates of Cryo-EM structure of the Arabidopsis thaliana C2S2... -
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Open data
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Basic information
| Entry | Database: PDB / ID: 9le8 | |||||||||||||||
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| Title | Coordinates of Cryo-EM structure of the Arabidopsis thaliana C2S2M2-type PSII supercomplex | |||||||||||||||
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Keywords | PHOTOSYNTHESIS / photosystem / PLANT PROTEIN | |||||||||||||||
| Function / homology | Function and homology informationphotosynthesis, light harvesting in photosystem II / photosystem II antenna complex / PSII associated light-harvesting complex II / nonphotochemical quenching / chloroplast stromal thylakoid / plastoglobule / response to high light intensity / thylakoid membrane / regulation of stomatal movement / chloroplast thylakoid ...photosynthesis, light harvesting in photosystem II / photosystem II antenna complex / PSII associated light-harvesting complex II / nonphotochemical quenching / chloroplast stromal thylakoid / plastoglobule / response to high light intensity / thylakoid membrane / regulation of stomatal movement / chloroplast thylakoid / vacuolar transport / thylakoid / photosynthesis, light harvesting / photosystem II oxygen evolving complex / photosystem II assembly / response to abscisic acid / oxygen evolving activity / photosystem II stabilization / photosystem II reaction center / photosystem II / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosynthetic electron transport chain / photosystem I / photosystem II / response to herbicide / plastid / chlorophyll binding / photosynthetic electron transport in photosystem II / : / phosphate ion binding / chloroplast thylakoid membrane / photosynthesis, light reaction / photosynthesis / chloroplast / electron transfer activity / protein stabilization / iron ion binding / protein domain specific binding / mRNA binding / heme binding / metal ion binding / nucleus / plasma membrane / cytosol Similarity search - Function | |||||||||||||||
| Biological species | ![]() | |||||||||||||||
| Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.37 Å | |||||||||||||||
Authors | Chen, S.J.B. / Wu, C. / Wu, J.H. / Sui, S.F. / Zhang, L.X. | |||||||||||||||
| Funding support | China, 1items
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Citation | Journal: To Be PublishedTitle: Coordinates of Cryo-EM structure of the Arabidopsis thaliana C2S2M2-type PSII supercomplex Authors: Chen, S.J.B. / Wu, C. / Wu, J.H. / Sui, S.F. / Zhang, L.X. | |||||||||||||||
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Structure visualization
| Structure viewer | Molecule: Molmil Jmol/JSmol |
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Downloads & links
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Download
| PDBx/mmCIF format | 9le8.cif.gz | 1.8 MB | Display | PDBx/mmCIF format |
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| PDB format | pdb9le8.ent.gz | Display | PDB format | |
| PDBx/mmJSON format | 9le8.json.gz | Tree view | PDBx/mmJSON format | |
| Others | Other downloads |
-Validation report
| Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/le/9le8 ftp://data.pdbj.org/pub/pdb/validation_reports/le/9le8 | HTTPS FTP |
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-Related structure data
| Related structure data | ![]() 63018MC ![]() 9le7C ![]() 66068 ![]() 66069 ![]() 66070 C: citing same article ( M: map data used to model this data |
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| Similar structure data | Similarity search - Function & homology F&H Search |
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Links
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Assembly
| Deposited unit | ![]()
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| 1 |
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Components
-Chlorophyll a-b binding protein, ... , 2 types, 6 molecules 9AAACAEABAF
| #1: Protein | Mass: 28193.934 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #3: Protein | Mass: 23198.287 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() |
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-Chlorophyll a-b binding protein ... , 4 types, 12 molecules 0ADVpAHANAQAUqARvAS
| #2: Protein | Mass: 26452.990 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #10: Protein | Mass: 24885.072 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) ![]() #16: Protein | Mass: 27302.855 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #17: Protein | Mass: 25232.754 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() |
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-Photosystem II ... , 13 types, 26 molecules JAJOAKPALQAMjAOIiKkoAPMmAGATUuWwAIAV
| #4: Protein | Mass: 38830.082 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #5: Protein | Mass: 56091.824 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #6: Protein | Mass: 50086.324 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #7: Protein | Mass: 39444.082 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #11: Protein | Mass: 7575.691 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #12: Protein/peptide | Mass: 4170.891 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #13: Protein/peptide | Mass: 4239.112 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #14: Protein/peptide | Mass: 4471.075 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #15: Protein/peptide | Mass: 3783.538 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #18: Protein/peptide | Mass: 3825.642 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #19: Protein/peptide | Mass: 3178.810 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #20: Protein | Mass: 6036.749 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #22: Protein | Mass: 6569.768 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() |
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-Cytochrome b559 subunit ... , 2 types, 4 molecules EeFf
| #8: Protein | Mass: 9393.501 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #9: Protein/peptide | Mass: 4428.228 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() |
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-Protein/peptide / Sugars , 2 types, 11 molecules Xx

| #21: Protein/peptide | Mass: 4186.916 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #35: Sugar | ChemComp-DGD / |
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-Non-polymers , 15 types, 435 molecules 




























| #23: Chemical | ChemComp-CHL / #24: Chemical | ChemComp-CLA / #25: Chemical | ChemComp-LUT / ( #26: Chemical | ChemComp-NEX / ( #27: Chemical | ChemComp-XAT / ( #28: Chemical | ChemComp-LHG / #29: Chemical | ChemComp-BCR / #30: Chemical | #31: Chemical | #32: Chemical | ChemComp-PHO / #33: Chemical | ChemComp-LMG / #34: Chemical | ChemComp-SQD / #36: Chemical | #37: Chemical | #38: Chemical | |
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-Details
| Has ligand of interest | Y |
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| Has protein modification | Y |
-Experimental details
-Experiment
| Experiment | Method: ELECTRON MICROSCOPY |
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| EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
| Component | Name: Cryo-EM structure of the Arabidopsis thaliana photosystem II(PSII-LHCII-ST2) Type: COMPLEX / Entity ID: #1-#22 / Source: NATURAL |
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| Source (natural) | Organism: ![]() |
| Buffer solution | pH: 7.4 |
| Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
| Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
| Experimental equipment | ![]() Model: Talos Arctica / Image courtesy: FEI Company |
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| Microscopy | Model: FEI TALOS ARCTICA |
| Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: OTHER |
| Electron lens | Mode: OTHER / Nominal defocus max: 2200 nm / Nominal defocus min: 1200 nm |
| Image recording | Electron dose: 50.5 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
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Processing
| EM software |
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| CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||
| 3D reconstruction | Resolution: 3.37 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 38544 / Symmetry type: POINT |
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FIELD EMISSION GUN