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- PDB-9l3o: Crystal structure of endo-processive xyloglucanase Xeg5A from Asp... -

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Basic information

Entry
Database: PDB / ID: 9l3o
TitleCrystal structure of endo-processive xyloglucanase Xeg5A from Aspergillus oryzae with XXLG
ComponentsGlycoside hydrolase superfamily
KeywordsHYDROLASE / Xyloglucanase / Complex
Function / homology
Function and homology information


glycogen biosynthetic process / beta-glucosidase activity / cellulose catabolic process / cell wall organization / cell surface / extracellular region
Similarity search - Function
Glycoside hydrolase, family 5, endoglucanase B / Carbohydrate binding X2 domain / Carbohydrate binding domain X2 / : / Glycoside hydrolase, family 5 / Cellulase (glycosyl hydrolase family 5) / Immunoglobulin E-set / Glycoside hydrolase superfamily / Immunoglobulin-like fold
Similarity search - Domain/homology
beta-D-glucopyranose / alpha-D-mannopyranose / DI(HYDROXYETHYL)ETHER / Glycoside hydrolase superfamily
Similarity search - Component
Biological speciesAspergillus oryzae RIB40 (mold)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å
AuthorsNakamichi, Y. / Shimada, N. / Watanabe, M. / Fujii, T. / Matsuzawa, T.
Funding support1items
OrganizationGrant numberCountry
Not funded
CitationJournal: J.Struct.Biol. / Year: 2025
Title: Structural insights into substrate recognition of tri-modular xyloglucanase from Aspergillus oryzae.
Authors: Nakamichi, Y. / Shimada, N. / Watanabe, M. / Fujii, T. / Yaoi, K. / Matsuzawa, T.
History
DepositionDec 19, 2024Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Jun 25, 2025Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Glycoside hydrolase superfamily
B: Glycoside hydrolase superfamily
hetero molecules


Theoretical massNumber of molelcules
Total (without water)155,23427
Polymers143,0002
Non-polymers12,23525
Water9,224512
1
A: Glycoside hydrolase superfamily
hetero molecules


Theoretical massNumber of molelcules
Total (without water)77,83814
Polymers71,5001
Non-polymers6,33813
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6200 Å2
ΔGint75 kcal/mol
Surface area23740 Å2
MethodPISA
2
B: Glycoside hydrolase superfamily
hetero molecules


Theoretical massNumber of molelcules
Total (without water)77,39613
Polymers71,5001
Non-polymers5,89712
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6080 Å2
ΔGint76 kcal/mol
Surface area23420 Å2
MethodPISA
Unit cell
Length a, b, c (Å)79.706, 105.896, 82.946
Angle α, β, γ (deg.)90.000, 91.604, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z

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Components

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Protein , 1 types, 2 molecules AB

#1: Protein Glycoside hydrolase superfamily


Mass: 71499.789 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Aspergillus oryzae RIB40 (mold) / Gene: Ao3042_05083 / Production host: Komagataella pastoris (fungus) / References: UniProt: I8IVP5

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Sugars , 8 types, 19 molecules

#2: Polysaccharide alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-[alpha-D-mannopyranose-(1-6)]alpha-D- ...alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 1721.527 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-2DManpa1-2[DManpa1-6]DManpa1-3[DManpa1-3[DManpa1-6]DManpa1-6]DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/3,10,9/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3-3-3-3-3-3-3/a4-b1_b4-c1_c3-d1_c6-h1_d2-e1_d6-g1_e2-f1_h3-i1_h6-j1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{[(2+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}[(6+1)][a-D-Manp]{}}[(6+1)][a-D-Manp]{[(3+1)][a-D-Manp]{}[(6+1)][a-D-Manp]{}}}}}}LINUCSPDB-CARE
#3: Polysaccharide alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D- ...alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 1235.105 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-2DManpa1-2DManpa1-3[DManpa1-6]DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/3,7,6/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3-3-3-3/a4-b1_b4-c1_c3-d1_c6-g1_d2-e1_e2-f1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{[(2+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}}[(6+1)][a-D-Manp]{}}}}}LINUCSPDB-CARE
#4: Polysaccharide alpha-D-xylopyranose-(1-6)-beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D- ...alpha-D-xylopyranose-(1-6)-beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-[beta-D-galactopyranose-(1-2)-alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-beta-D-glucopyranose


Type: oligosaccharide / Mass: 1225.064 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DXylpa1-6DGlcpb1-4[DXylpa1-6]DGlcpb1-4[DGalpb1-2DXylpa1-6]DGlcpb1-4DGlcpb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,8,7/[a2122h-1b_1-5][a212h-1a_1-5][a2112h-1b_1-5]/1-1-1-1-2-2-2-3/a4-b1_b4-c1_b6-g1_c4-d1_c6-f1_d6-e1_g2-h1WURCSPDB2Glycan 1.1.0
[][b-D-Glcp]{[(4+1)][b-D-Glcp]{[(4+1)][b-D-Glcp]{[(4+1)][b-D-Glcp]{[(6+1)][a-D-Xylp]{}}[(6+1)][a-D-Xylp]{}}[(6+1)][a-D-Xylp]{[(2+1)][b-D-Galp]{}}}}LINUCSPDB-CARE
#5: Polysaccharide beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta- ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 586.542 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/2,3,2/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5]/1-1-2/a4-b1_b4-c1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{}}}}LINUCSPDB-CARE
#6: Polysaccharide alpha-D-xylopyranose-(1-6)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-[beta-D- ...alpha-D-xylopyranose-(1-6)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-[beta-D-galactopyranose-(1-2)-alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-beta-D-glucopyranose


Type: oligosaccharide / Mass: 1092.949 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DXylpa1-6DGlcpb1-4DGlcpb1-4[DGalpb1-2DXylpa1-6]DGlcpb1-4DGlcpb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,7,6/[a2122h-1b_1-5][a212h-1a_1-5][a2112h-1b_1-5]/1-1-1-1-2-2-3/a4-b1_b4-c1_b6-f1_c4-d1_d6-e1_f2-g1WURCSPDB2Glycan 1.1.0
[][b-D-Glcp]{[(4+1)][b-D-Glcp]{[(4+1)][b-D-Glcp]{[(4+1)][b-D-Glcp]{[(6+1)][a-D-Xylp]{}}}[(6+1)][a-D-Xylp]{[(2+1)][b-D-Galp]{}}}}LINUCSPDB-CARE
#8: Sugar ChemComp-MAN / alpha-D-mannopyranose / alpha-D-mannose / D-mannose / mannose


Type: D-saccharide, alpha linking / Mass: 180.156 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C6H12O6
IdentifierTypeProgram
DManpaCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
a-D-mannopyranoseCOMMON NAMEGMML 1.0
a-D-ManpIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
ManSNFG CARBOHYDRATE SYMBOLGMML 1.0
#9: Sugar
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 7 / Source method: obtained synthetically / Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0
#10: Sugar ChemComp-BGC / beta-D-glucopyranose / beta-D-glucose / D-glucose / glucose


Type: D-saccharide, beta linking / Mass: 180.156 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C6H12O6 / Feature type: SUBJECT OF INVESTIGATION
IdentifierTypeProgram
DGlcpbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
b-D-glucopyranoseCOMMON NAMEGMML 1.0
b-D-GlcpIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 4 types, 518 molecules

#7: Chemical ChemComp-PG4 / TETRAETHYLENE GLYCOL


Mass: 194.226 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C8H18O5 / Comment: precipitant*YM
#11: Chemical ChemComp-PEG / DI(HYDROXYETHYL)ETHER


Mass: 106.120 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C4H10O3
#12: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mg
#13: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 512 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.45 Å3/Da / Density % sol: 49.73 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / Details: 0.2 M sodium acetate, 20% (w/v) PEG3350

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Data collection

DiffractionMean temperature: 100 K / Ambient temp details: LN2 / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SPring-8 / Beamline: BL44XU / Wavelength: 0.9 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jul 18, 2023
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9 Å / Relative weight: 1
ReflectionResolution: 2→44.64 Å / Num. obs: 92363 / % possible obs: 98.9 % / Redundancy: 3.5 % / Biso Wilson estimate: 34.14 Å2 / CC1/2: 0.996 / Rmerge(I) obs: 0.111 / Rpim(I) all: 0.071 / Rrim(I) all: 0.132 / Net I/σ(I): 8.6
Reflection shellResolution: 2→2.07 Å / Redundancy: 3.6 % / Mean I/σ(I) obs: 1.1 / Num. unique obs: 9104 / CC1/2: 0.559 / % possible all: 97.5

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Processing

Software
NameVersionClassification
PHENIX1.21.1_5286refinement
XDSJan 10, 2022data reduction
XDSJan 10, 2022data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2→39.84 Å / SU ML: 0.2862 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 34.1856
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2479 4608 5 %
Rwork0.2037 87488 -
obs0.2059 92096 98.63 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 43.89 Å2
Refinement stepCycle: LAST / Resolution: 2→39.84 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms8630 0 813 512 9955
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00549689
X-RAY DIFFRACTIONf_angle_d0.852713244
X-RAY DIFFRACTIONf_chiral_restr0.04851688
X-RAY DIFFRACTIONf_plane_restr0.00621578
X-RAY DIFFRACTIONf_dihedral_angle_d17.54393920
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2-2.020.471460.46342773X-RAY DIFFRACTION94.1
2.02-2.040.45071550.43212918X-RAY DIFFRACTION98.18
2.04-2.070.46771520.42542878X-RAY DIFFRACTION97.74
2.07-2.090.49211500.40912829X-RAY DIFFRACTION97.7
2.09-2.120.42071510.38632889X-RAY DIFFRACTION98.38
2.12-2.150.40561530.36292889X-RAY DIFFRACTION98.19
2.15-2.180.39911530.35762903X-RAY DIFFRACTION98.17
2.18-2.210.38871540.33612938X-RAY DIFFRACTION98.38
2.21-2.250.38491530.31892885X-RAY DIFFRACTION97.68
2.25-2.290.34481520.30222875X-RAY DIFFRACTION98.25
2.29-2.320.35171510.30942873X-RAY DIFFRACTION98.28
2.32-2.370.35971530.29092935X-RAY DIFFRACTION98.75
2.37-2.410.30691540.2592909X-RAY DIFFRACTION99.09
2.41-2.460.23131530.22732933X-RAY DIFFRACTION99.01
2.46-2.520.28811530.22682919X-RAY DIFFRACTION99.35
2.52-2.570.24991550.21182937X-RAY DIFFRACTION99.29
2.57-2.640.26471540.2012932X-RAY DIFFRACTION99.39
2.64-2.710.25551550.19672928X-RAY DIFFRACTION99.39
2.71-2.790.23651530.20152914X-RAY DIFFRACTION99.16
2.79-2.880.23171550.20072943X-RAY DIFFRACTION99.2
2.88-2.980.24261550.2032936X-RAY DIFFRACTION99.07
2.98-3.10.24891550.19892936X-RAY DIFFRACTION99.23
3.1-3.240.20971530.19532916X-RAY DIFFRACTION98.75
3.24-3.410.27511540.19322929X-RAY DIFFRACTION98.97
3.41-3.630.22921560.1732958X-RAY DIFFRACTION99.24
3.63-3.910.18151550.16242937X-RAY DIFFRACTION99.45
3.91-4.30.19231550.13552948X-RAY DIFFRACTION99.49
4.3-4.920.16561560.12632976X-RAY DIFFRACTION99.18
4.92-6.20.18441560.14772954X-RAY DIFFRACTION99.3
6.2-39.840.20661580.16362998X-RAY DIFFRACTION98.56
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
13.977072181620.3102073083360.292850758274.76547109088-1.842694375833.67027578828-0.1875421561220.7055637839160.133427017834-0.4973986646070.2707347089260.0454755603383-0.1410635726820.10102473027-1.00613778282E-50.363366388944-0.0781486068698-0.04819974090230.332659645991-0.0407088710090.17991801838311.53462960957.9718705766114.7473106772
23.042216403090.446929780243-1.4837075361.56768501805-0.4680791248571.62629681124-0.0168390485950.08072730126030.0853441261193-0.08003019534260.0461993308829-0.0393628472264-0.05969522005470.01505475383-0.02576337588070.270267377892-0.00601930998137-0.02986575183960.202144594444-0.008634109852230.20935966937810.28157476246.1498667313327.4579872628
33.339819412330.6284261399890.1689361849963.15151190728-0.1107582365093.19542349897-0.02042907626360.2127428982-0.176058996447-0.2621513151160.0947545591050.5535353183270.273431404399-0.599778989897-0.08710470582020.238397729922-0.0502556931442-0.04015558048190.4015405181230.01144549925360.357862029445-22.7179408312-9.3148232395933.035424416
43.71695672768-0.203419061502-0.0948810547065.233458983790.5971977246893.46598480048-0.0126153709959-0.438408317596-0.06857198738130.2863568779460.0708459945823-0.3482670959590.04800031940950.274150335965-0.02844991960920.216656509912-0.0149017433375-0.01604951489070.2797556038190.0209609660990.211718191234-2.0192312211-9.7507821940253.952173296
52.192712018170.7225789436660.08686191440052.97964798550.4869596007094.170945509440.144405150652-0.06518557956680.269196515860.2037805260170.1110221541860.401345360528-0.23102551529-0.266174554927-0.2539823606040.2151772569130.0416909313880.07321202844220.2646717894690.03575113022390.37338698295419.4002811822-26.359514336916.7280835631
63.6003485321-0.346220930539-1.511324392153.320014227930.2560302789362.632688858630.0401874633992-0.286079498545-0.164944117870.4657474532290.01557166820040.1021213446960.1052794308870.114695454871-0.06470083288350.291905368398-0.0175915143719-0.02473776453960.2309646766990.03240906725720.20464690312824.6099640136-35.330893652221.6933638199
75.371495410232.78762174893-4.166108188713.03111164707-2.243972329653.9503808879-0.187783995149-0.0146575712403-0.387212702649-0.209841686601-0.0627066940739-0.1724170803410.331354806199-0.1138457309320.2580680146770.2475156190490.020422359363-0.05524078899710.21107219855-0.00989005745150.25454673218731.3206001126-36.9846784360.975677134097
81.397371796460.808457512587-2.099599955010.620576743325-1.542854866094.566475598950.04848385248290.2500197902640.124189129089-0.2579221789490.1409699738680.205028725359-0.109279001113-0.449703052299-0.1591436224930.2361721687430.0266834968581-0.04585864497740.3202773540730.03512947152840.41210625032523.6198599846-25.746962306-2.64381358323
93.10450533465-0.3496504025580.5861036663682.44120535957-0.5253179527432.91253580824-0.06983038955090.6004516782180.198608929702-0.4536704919860.1528637564450.333142089082-0.157841380364-0.206168540515-0.07965532888460.340709268463-0.0444613497117-0.07797310697910.4187221570130.06693678207470.26726341010831.3125857535-17.443922415-21.8241891514
108.112971940723.629858213410.8024355059984.26364435820.6926283252161.47418018925-0.110370412873-0.3883294420290.3748206739050.140853295381-0.00864088132256-0.108615348708-0.5060798761160.2638117209130.1697749792820.3227920884980.05865879984680.0590694665310.191678185661-0.04674150527370.28310702967248.4895919234-8.99916909751-2.54055078221
111.596346117110.369273107411-1.53656584794.367954786580.3208368394512.11469623322-0.0120570163178-0.190533823148-0.1018917508040.2558214611560.00208120915085-0.1999340945230.006398353630220.3654624440820.004459616217250.227397752933-0.00135136143265-0.07065005670190.2885748585730.0009860176094150.20156570485552.4387736207-18.4051982888-1.34897260772
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 20 through 49 )AA20 - 491 - 30
22chain 'A' and (resid 50 through 353 )AA50 - 35331 - 334
33chain 'A' and (resid 354 through 455 )AA354 - 455335 - 436
44chain 'A' and (resid 456 through 569 )AA456 - 569437 - 550
55chain 'B' and (resid 20 through 99 )BP20 - 991 - 80
66chain 'B' and (resid 100 through 223 )BP100 - 22381 - 204
77chain 'B' and (resid 224 through 311 )BP224 - 311205 - 292
88chain 'B' and (resid 312 through 353 )BP312 - 353293 - 334
99chain 'B' and (resid 354 through 448 )BP354 - 448335 - 429
1010chain 'B' and (resid 449 through 478 )BP449 - 478430 - 459
1111chain 'B' and (resid 479 through 569 )BP479 - 569460 - 550

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