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- PDB-9kag: Cryo-EM structure of the SPS3-FBN5 complex in a 2:2 state (class 4) -

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Basic information

Entry
Database: PDB / ID: 9kag
TitleCryo-EM structure of the SPS3-FBN5 complex in a 2:2 state (class 4)
Components
  • Fibrillin protein 5 homolog
  • Probable solanesyl-diphosphate synthase 3, chloroplastic
KeywordsTRANSFERASE / chloroplast
Function / homology
Function and homology information


all-trans-nonaprenyl diphosphate synthase [geranyl-diphosphate specific] / all-trans-nonaprenyl-diphosphate synthase (geranyl-diphosphate specific) activity / plastoquinone biosynthetic process / prenyltransferase activity / isoprenoid biosynthetic process / chloroplast thylakoid membrane / chloroplast / metal ion binding
Similarity search - Function
Plastid lipid-associated protein/fibrillin conserved domain / Plastid-lipid-associated protein / PAP_fibrillin / Polyprenyl synthases signature 1. / Polyprenyl synthases signature 2. / Polyprenyl synthetase, conserved site / Polyprenyl synthetase / Polyprenyl synthetase / Isoprenoid synthase domain superfamily
Similarity search - Domain/homology
Probable solanesyl-diphosphate synthase 3, chloroplastic / Fibrillin protein 5 homolog
Similarity search - Component
Biological speciesOryza sativa Japonica Group (Japanese rice)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsXiao, H. / Wang, Y.-W. / Zhu, P. / Yang, G.-F.
Funding support China, 1items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC) China
CitationJournal: To Be Published
Title: Cryo-EM structure of the SPS3-FBN5 complex in a 2:2 state (class 4)
Authors: Xiao, H. / Wang, Y.-W. / Zhu, P. / Yang, G.-F.
History
DepositionOct 28, 2024Deposition site: PDBJ / Processing site: PDBC
Revision 1.0May 6, 2026Provider: repository / Type: Initial release
Revision 1.0May 6, 2026Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release
Revision 1.0May 6, 2026Data content type: FSC / Data content type: FSC / Provider: repository / Type: Initial release
Revision 1.0May 6, 2026Data content type: Half map / Part number: 1 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0May 6, 2026Data content type: Half map / Part number: 2 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0May 6, 2026Data content type: Image / Data content type: Image / Provider: repository / Type: Initial release
Revision 1.0May 6, 2026Data content type: Primary map / Data content type: Primary map / Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Fibrillin protein 5 homolog
B: Probable solanesyl-diphosphate synthase 3, chloroplastic
C: Fibrillin protein 5 homolog
D: Probable solanesyl-diphosphate synthase 3, chloroplastic


Theoretical massNumber of molelcules
Total (without water)117,7204
Polymers117,7204
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

#1: Protein Fibrillin protein 5 homolog / OsFBN5 / Plastid-lipid-associated protein 7 homolog / chloroplastic


Mass: 22999.350 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Oryza sativa Japonica Group (Japanese rice)
Gene: FBN5, Os04g0422000, LOC_Os04g34460, OsJ_14801, OSJNBa0028I23.21
Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q0JD85
#2: Protein Probable solanesyl-diphosphate synthase 3, chloroplastic / OsSPS3 / Probable all-trans-nonaprenyl-diphosphate synthase 3 (geranyl-diphosphate specific)


Mass: 35860.766 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Oryza sativa Japonica Group (Japanese rice)
Gene: SPS3, Os12g0271700, LOC_Os12g17320, OsJ_1767i3 / Production host: Escherichia coli BL21(DE3) (bacteria)
References: UniProt: Q0INZ4, all-trans-nonaprenyl diphosphate synthase [geranyl-diphosphate specific]
Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Cryo-EM structure of the SPS3-FBN5 complex in a 2:2 state (class 4)
Type: COMPLEX / Entity ID: all / Source: RECOMBINANT
Source (natural)Organism: Oryza sativa subsp. japonica (Japanese rice)
Source (recombinant)Organism: Escherichia coli BL21(DE3) (bacteria)
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 1800 nm / Nominal defocus min: 1400 nm
Image recordingElectron dose: 49.43 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k)

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Processing

EM softwareName: PHENIX / Category: model refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 147077 / Symmetry type: POINT
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.0048368
ELECTRON MICROSCOPYf_angle_d0.69711300
ELECTRON MICROSCOPYf_dihedral_angle_d4.0171138
ELECTRON MICROSCOPYf_chiral_restr0.0431330
ELECTRON MICROSCOPYf_plane_restr0.0051452

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