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- PDB-9jo9: CRP-HCAb3 complex -

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Basic information

Entry
Database: PDB / ID: 9jo9
TitleCRP-HCAb3 complex
Components
  • C-reactive protein
  • CRP specific recognition heavy chain antibodie 3 (HCAb3)
KeywordsIMMUNE SYSTEM / C reactive protein and antibody complex
Function / homology
Function and homology information


regulation of interleukin-8 production / opsonization / complement component C1q complex binding / low-density lipoprotein particle binding / vasoconstriction / choline binding / negative regulation of mononuclear cell proliferation / Classical antibody-mediated complement activation / low-density lipoprotein particle receptor binding / negative regulation of macrophage derived foam cell differentiation ...regulation of interleukin-8 production / opsonization / complement component C1q complex binding / low-density lipoprotein particle binding / vasoconstriction / choline binding / negative regulation of mononuclear cell proliferation / Classical antibody-mediated complement activation / low-density lipoprotein particle receptor binding / negative regulation of macrophage derived foam cell differentiation / negative regulation of lipid storage / positive regulation of superoxide anion generation / acute-phase response / defense response to Gram-positive bacterium / inflammatory response / innate immune response / calcium ion binding / positive regulation of gene expression / extracellular space / extracellular region / identical protein binding
Similarity search - Function
: / Pentaxin, conserved site / Pentraxin domain signature. / Pentaxin family / Pentraxin / C-reactive protein / pentaxin family / Pentraxin-related / Pentraxin (PTX) domain profile. / Concanavalin A-like lectin/glucanase domain superfamily
Similarity search - Domain/homology
Biological speciesVicugna pacos (alpaca)
Homo sapiens (human)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.65 Å
AuthorsLi, Y.J.
Funding support1items
OrganizationGrant numberCountry
Not funded
CitationJournal: To Be Published
Title: CRP-HCAb3 complex
Authors: Li, Y.J.
History
DepositionSep 24, 2024Deposition site: PDBJ / Processing site: PDBC
Revision 1.0Oct 22, 2025Provider: repository / Type: Initial release
Revision 1.0Oct 22, 2025Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release
Revision 1.0Oct 22, 2025Data content type: FSC / Data content type: FSC / Provider: repository / Type: Initial release
Revision 1.0Oct 22, 2025Data content type: Half map / Part number: 1 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0Oct 22, 2025Data content type: Half map / Part number: 2 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0Oct 22, 2025Data content type: Image / Data content type: Image / Provider: repository / Type: Initial release
Revision 1.0Oct 22, 2025Data content type: Primary map / Data content type: Primary map / Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
O: CRP specific recognition heavy chain antibodie 3 (HCAb3)
S: C-reactive protein
A: CRP specific recognition heavy chain antibodie 3 (HCAb3)
B: C-reactive protein
C: CRP specific recognition heavy chain antibodie 3 (HCAb3)
D: C-reactive protein
E: CRP specific recognition heavy chain antibodie 3 (HCAb3)
F: C-reactive protein
G: CRP specific recognition heavy chain antibodie 3 (HCAb3)
H: C-reactive protein
I: CRP specific recognition heavy chain antibodie 3 (HCAb3)
J: C-reactive protein
K: CRP specific recognition heavy chain antibodie 3 (HCAb3)
L: C-reactive protein
M: CRP specific recognition heavy chain antibodie 3 (HCAb3)
N: C-reactive protein
P: CRP specific recognition heavy chain antibodie 3 (HCAb3)
Q: C-reactive protein
R: CRP specific recognition heavy chain antibodie 3 (HCAb3)
T: C-reactive protein


Theoretical massNumber of molelcules
Total (without water)372,95920
Polymers372,95920
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

#1: Antibody
CRP specific recognition heavy chain antibodie 3 (HCAb3)


Mass: 14227.812 Da / Num. of mol.: 10
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Vicugna pacos (alpaca) / Production host: Homo sapiens (human)
#2: Protein
C-reactive protein / C-reactive protein pentamer


Mass: 23068.039 Da / Num. of mol.: 10
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: CRP, PTX1 / Production host: Homo sapiens (human) / References: UniProt: P02741
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: C5 symmetry pentamer complex of CRP antigen and antibody4
Type: COMPLEX / Entity ID: all / Source: RECOMBINANT
Source (natural)
IDEntity assembly-IDOrganismNcbi tax-ID
21Homo sapiens (human)9606
31Vicugna pacos (alpaca)30538
Source (recombinant)Organism: Homo sapiens (human)
Buffer solutionpH: 8 / Details: 20mM Tris-HCl
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 2500 nm / Nominal defocus min: 1500 nm
Image recordingElectron dose: 30 e/Å2 / Film or detector model: GATAN K3 (6k x 4k)

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Processing

EM softwareName: PHENIX / Category: model refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
SymmetryPoint symmetry: C5 (5 fold cyclic)
3D reconstructionResolution: 3.65 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 249262 / Symmetry type: POINT
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.00326790
ELECTRON MICROSCOPYf_angle_d0.82636350
ELECTRON MICROSCOPYf_dihedral_angle_d6.5863696
ELECTRON MICROSCOPYf_chiral_restr0.0693900
ELECTRON MICROSCOPYf_plane_restr0.0044670

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