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Open data
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Basic information
Entry | Database: PDB / ID: 9j4e | |||||||||||||||||||||
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Title | Cryo-EM structure of P25alpha full-length fibril | |||||||||||||||||||||
![]() | Tubulin polymerization-promoting protein | |||||||||||||||||||||
![]() | CYTOSOLIC PROTEIN / PROTEIN FIBRIL / amyloid | |||||||||||||||||||||
Function / homology | ![]() microtubule nucleation by microtubule organizing center / postsynaptic Golgi apparatus / myelin assembly / astral microtubule organization / positive regulation of myelination / microtubule bundle formation / oligodendrocyte development / positive regulation of protein polymerization / negative regulation of tubulin deacetylation / microtubule organizing center ...microtubule nucleation by microtubule organizing center / postsynaptic Golgi apparatus / myelin assembly / astral microtubule organization / positive regulation of myelination / microtubule bundle formation / oligodendrocyte development / positive regulation of protein polymerization / negative regulation of tubulin deacetylation / microtubule organizing center / microtubule polymerization / oligodendrocyte differentiation / tubulin binding / regulation of microtubule cytoskeleton organization / positive regulation of protein-containing complex assembly / mitotic spindle / microtubule binding / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / microtubule / cell division / GTPase activity / perinuclear region of cytoplasm / magnesium ion binding / protein homodimerization activity / mitochondrion / nucleus / cytosol / cytoplasm Similarity search - Function | |||||||||||||||||||||
Biological species | ![]() | |||||||||||||||||||||
Method | ELECTRON MICROSCOPY / helical reconstruction / cryo EM / Resolution: 3.32 Å | |||||||||||||||||||||
![]() | Xia, W.C. / Sun, Y.P. / Huang, C.A. / Liu, C. | |||||||||||||||||||||
Funding support | 1items
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![]() | ![]() Title: Cryo-EM structure of P25alpha core fibril Authors: Xia, W.C. / Sun, Y.P. / Huang, C.A. / Liu, C. | |||||||||||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 64.2 KB | Display | ![]() |
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PDB format | ![]() | 39.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 935.7 KB | Display | ![]() |
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Full document | ![]() | 941 KB | Display | |
Data in XML | ![]() | 19.9 KB | Display | |
Data in CIF | ![]() | 27.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 61133MC ![]() 9j4dC ![]() 9j4fC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Components
#1: Protein | Mass: 23739.824 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: O94811, Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement Has protein modification | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: FILAMENT / 3D reconstruction method: helical reconstruction |
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Sample preparation
Component | Name: Cryo-EM structure of P25alpha core fibril / Type: ORGANELLE OR CELLULAR COMPONENT / Entity ID: all / Source: RECOMBINANT |
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Source (natural) | Organism: ![]() |
Source (recombinant) | Organism: ![]() ![]() |
Buffer solution | pH: 8.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 1000 nm |
Image recording | Electron dose: 55 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
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Processing
CTF correction | Type: NONE |
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Helical symmerty | Angular rotation/subunit: 179.4 ° / Axial rise/subunit: 2.42 Å / Axial symmetry: C1 |
3D reconstruction | Resolution: 3.32 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 99833 / Symmetry type: HELICAL |