+Open data
-Basic information
Entry | Database: PDB / ID: 9iiw | ||||||
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Title | A local Cryo-EM structure of Bitter taste receptor TAS2R14 | ||||||
Components | Taste receptor type 2 member 14 | ||||||
Keywords | MEMBRANE PROTEIN / GPCR | ||||||
Function / homology | Function and homology information bitter taste receptor activity / taste receptor activity / detection of chemical stimulus involved in sensory perception of bitter taste / Class C/3 (Metabotropic glutamate/pheromone receptors) / G protein-coupled receptor activity / Sensory perception of sweet, bitter, and umami (glutamate) taste / G alpha (i) signalling events / G protein-coupled receptor signaling pathway / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.15 Å | ||||||
Authors | Yuan, Q. / Duan, J. / Tao, L. / Xu, E.H. | ||||||
Funding support | China, 1items
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Citation | Journal: Cell Res / Year: 2024 Title: Bitter taste receptor TAS2R14 activation and G protein assembly by an intracellular agonist. Authors: Lele Tao / Dongxue Wang / Qingning Yuan / Fenghui Zhao / Yu Zhang / Tianyuan Du / Shiyi Shen / H Eric Xu / Yi Li / Dehua Yang / Jia Duan / | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 9iiw.cif.gz | 68 KB | Display | PDBx/mmCIF format |
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PDB format | pdb9iiw.ent.gz | Display | PDB format | |
PDBx/mmJSON format | 9iiw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ii/9iiw ftp://data.pdbj.org/pub/pdb/validation_reports/ii/9iiw | HTTPS FTP |
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-Related structure data
Related structure data | 60607MC 9iixC 9ij9C 9ijaC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 36200.070 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: TAS2R14 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: Q9NYV8 | ||
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#2: Chemical | Mass: 321.261 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C13H10F3N7 / Feature type: SUBJECT OF INVESTIGATION Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: local refinement of TAS2R14 / Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT |
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Source (natural) | Organism: Homo sapiens (human) |
Source (recombinant) | Organism: Spodoptera frugiperda (fall armyworm) |
Buffer solution | pH: 7.04 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 18000 nm / Nominal defocus min: 8000 nm / Cs: 2.7 mm |
Image recording | Electron dose: 15 e/Å2 / Film or detector model: GATAN K3 BIOCONTINUUM (6k x 4k) |
-Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
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3D reconstruction | Resolution: 3.15 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 354147 / Symmetry type: POINT |