[English] 日本語
Yorodumi
- PDB-9h2t: Crystal structure of YTHDC1 in complex with AI_04 -

+
Open data


ID or keywords:

Loading...

-
Basic information

Entry
Database: PDB / ID: 9h2t
TitleCrystal structure of YTHDC1 in complex with AI_04
ComponentsYTH domain-containing protein 1
KeywordsRNA BINDING PROTEIN / YTHDC1 / inhibitor / complex / reader
Function / homology
Function and homology information


primary follicle stage / mRNA alternative polyadenylation / mRNA splice site recognition / dosage compensation by inactivation of X chromosome / N6-methyladenosine-containing RNA reader activity / regulation of mRNA splicing, via spliceosome / post-transcriptional regulation of gene expression / regulation of alternative mRNA splicing, via spliceosome / mRNA export from nucleus / mRNA splicing, via spliceosome ...primary follicle stage / mRNA alternative polyadenylation / mRNA splice site recognition / dosage compensation by inactivation of X chromosome / N6-methyladenosine-containing RNA reader activity / regulation of mRNA splicing, via spliceosome / post-transcriptional regulation of gene expression / regulation of alternative mRNA splicing, via spliceosome / mRNA export from nucleus / mRNA splicing, via spliceosome / spermatogenesis / in utero embryonic development / nuclear speck / mRNA binding / RNA binding / nucleoplasm / nucleus / plasma membrane
Similarity search - Function
YTH domain containing protein / YTH domain / YT521-B-like domain / YTH domain profile.
Similarity search - Domain/homology
: / YTH domain-containing protein 1
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.31 Å
AuthorsBedi, R.K. / Caflisch, A.
Funding support Switzerland, 1items
OrganizationGrant numberCountry
Swiss National Science Foundation310030B_189363 Switzerland
CitationJournal: To Be Published
Title: Crystal structure of YTHDC1 in complex with AI_04
Authors: Bedi, R.K. / Caflisch, A.
History
DepositionOct 15, 2024Deposition site: PDBE / Processing site: PDBE
Revision 1.0Aug 27, 2025Provider: repository / Type: Initial release

-
Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

-
Assembly

Deposited unit
A: YTH domain-containing protein 1
B: YTH domain-containing protein 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)38,96913
Polymers37,6482
Non-polymers1,32111
Water9,404522
1
A: YTH domain-containing protein 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)19,5327
Polymers18,8241
Non-polymers7096
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: YTH domain-containing protein 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)19,4366
Polymers18,8241
Non-polymers6125
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)39.740, 103.680, 42.280
Angle α, β, γ (deg.)90.000, 105.263, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z

-
Components

#1: Protein YTH domain-containing protein 1 / Splicing factor YT521 / YT521-B


Mass: 18823.826 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: YTHDC1, KIAA1966, YT521 / Production host: Escherichia coli (E. coli) / References: UniProt: Q96MU7
#2: Chemical ChemComp-A1IR3 / 6-cyclopropyl-3-phenyl-1~{H}-pyrimidine-2,4-dione


Mass: 228.247 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C13H12N2O2 / Feature type: SUBJECT OF INVESTIGATION
#3: Chemical
ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 9 / Source method: obtained synthetically / Formula: SO4
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 522 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY
Has protein modificationN

-
Experimental details

-
Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

-
Sample preparation

CrystalDensity Matthews: 2.23 Å3/Da / Density % sol: 44.89 %
Crystal growTemperature: 295 K / Method: vapor diffusion, hanging drop
Details: 25% PEG 3350, 0.2M Ammonium Sulphate, 0.1M Bis-Tris pH 6.5

-
Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X06SA / Wavelength: 1 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: May 13, 2022
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 1.31→40.8 Å / Num. obs: 76333 / % possible obs: 95.7 % / Redundancy: 3.43 % / Biso Wilson estimate: 13.61 Å2 / CC1/2: 0.994 / Net I/σ(I): 17.01
Reflection shellResolution: 1.31→1.39 Å / Num. unique obs: 11988 / CC1/2: 0.975

-
Processing

Software
NameVersionClassification
PHENIX1.21.1_5286refinement
XDSdata reduction
XSCALEdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.31→40.79 Å / SU ML: 0.149 / Cross valid method: FREE R-VALUE / σ(F): 1.38 / Phase error: 21.8452
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2104 3817 5 %
Rwork0.187 72497 -
obs0.1882 76314 95.76 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 18.31 Å2
Refinement stepCycle: LAST / Resolution: 1.31→40.79 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms2564 0 79 522 3165
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00592741
X-RAY DIFFRACTIONf_angle_d0.86243725
X-RAY DIFFRACTIONf_chiral_restr0.0814401
X-RAY DIFFRACTIONf_plane_restr0.0066461
X-RAY DIFFRACTIONf_dihedral_angle_d13.4839983
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.31-1.320.35081270.30962410X-RAY DIFFRACTION86.35
1.32-1.340.30291360.29922594X-RAY DIFFRACTION94.76
1.34-1.360.29311430.29062708X-RAY DIFFRACTION95.26
1.36-1.380.31031410.26812684X-RAY DIFFRACTION96.25
1.38-1.40.28851410.25672681X-RAY DIFFRACTION96.02
1.4-1.420.28611440.24182738X-RAY DIFFRACTION95.97
1.42-1.440.23571340.22672541X-RAY DIFFRACTION93.04
1.44-1.470.23641380.21872630X-RAY DIFFRACTION93.2
1.47-1.50.22781420.21062683X-RAY DIFFRACTION96.75
1.5-1.520.20741430.19832722X-RAY DIFFRACTION97.15
1.52-1.560.22311440.18992739X-RAY DIFFRACTION97.07
1.56-1.590.21511420.18582704X-RAY DIFFRACTION96.97
1.59-1.630.22771440.18282725X-RAY DIFFRACTION96.96
1.63-1.670.19661440.17872734X-RAY DIFFRACTION97.59
1.67-1.710.19261430.17332726X-RAY DIFFRACTION97.62
1.71-1.760.20011440.17182737X-RAY DIFFRACTION97.5
1.76-1.820.1961440.17442730X-RAY DIFFRACTION97.49
1.82-1.880.18311430.16752712X-RAY DIFFRACTION96.78
1.88-1.960.20061350.17482570X-RAY DIFFRACTION92.26
1.96-2.050.17771410.17142676X-RAY DIFFRACTION95.2
2.05-2.160.17631450.16552763X-RAY DIFFRACTION98.18
2.16-2.290.19861460.17172764X-RAY DIFFRACTION98.91
2.29-2.470.22741480.17512812X-RAY DIFFRACTION99.03
2.47-2.720.20851450.18542756X-RAY DIFFRACTION98.27
2.72-3.110.19281450.18162761X-RAY DIFFRACTION97.58
3.11-3.920.19931350.17072558X-RAY DIFFRACTION91.04
3.92-40.790.22681400.20292639X-RAY DIFFRACTION92.29

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi

Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
  • The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

Read more