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- PDB-9gio: Crystal structure of the VHL-EloC-EloB complex with a covalent co... -

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Basic information

Entry
Database: PDB / ID: 9gio
TitleCrystal structure of the VHL-EloC-EloB complex with a covalent compound bound to C77 of VHL.
Components
  • Elongin-B
  • Isoform 2 of Elongin-C
  • von Hippel-Lindau disease tumor suppressor
KeywordsLIGASE / VHL / covalent / fragment / E3 ligase
Function / homology
Function and homology information


regulation of cellular response to hypoxia / negative regulation of receptor signaling pathway via JAK-STAT / RHOBTB3 ATPase cycle / target-directed miRNA degradation / elongin complex / VCB complex / Replication of the SARS-CoV-1 genome / transcription elongation factor activity / Cul5-RING ubiquitin ligase complex / ubiquitin-dependent protein catabolic process via the C-end degron rule pathway ...regulation of cellular response to hypoxia / negative regulation of receptor signaling pathway via JAK-STAT / RHOBTB3 ATPase cycle / target-directed miRNA degradation / elongin complex / VCB complex / Replication of the SARS-CoV-1 genome / transcription elongation factor activity / Cul5-RING ubiquitin ligase complex / ubiquitin-dependent protein catabolic process via the C-end degron rule pathway / SUMOylation of ubiquitinylation proteins / Cul2-RING ubiquitin ligase complex / negative regulation of DNA-templated DNA replication / negative regulation of transcription elongation by RNA polymerase II / Pausing and recovery of Tat-mediated HIV elongation / Tat-mediated HIV elongation arrest and recovery / HIV elongation arrest and recovery / Pausing and recovery of HIV elongation / negative regulation of signal transduction / Tat-mediated elongation of the HIV-1 transcript / Formation of HIV-1 elongation complex containing HIV-1 Tat / cell morphogenesis / Formation of HIV elongation complex in the absence of HIV Tat / RNA Polymerase II Transcription Elongation / ubiquitin-like ligase-substrate adaptor activity / Formation of RNA Pol II elongation complex / ribosome-associated ubiquitin-dependent protein catabolic process / RNA Polymerase II Pre-transcription Events / negative regulation of TORC1 signaling / negative regulation of autophagy / transcription corepressor binding / protein serine/threonine kinase binding / TP53 Regulates Transcription of DNA Repair Genes / positive regulation of cell differentiation / transcription initiation at RNA polymerase II promoter / transcription elongation by RNA polymerase II / Inactivation of CSF3 (G-CSF) signaling / Vif-mediated degradation of APOBEC3G / Evasion by RSV of host interferon responses / Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha / regulation of gene expression / Regulation of expression of SLITs and ROBOs / ubiquitin-protein transferase activity / Ribosome Quality Control (RQC) complex extracts and degrades nascent peptide / transcription corepressor activity / positive regulation of proteasomal ubiquitin-dependent protein catabolic process / Antigen processing: Ubiquitination & Proteasome degradation / Neddylation / protein-containing complex assembly / Replication of the SARS-CoV-2 genome / DNA-binding transcription factor binding / amyloid fibril formation / cellular response to hypoxia / proteasome-mediated ubiquitin-dependent protein catabolic process / molecular adaptor activity / protein stabilization / protein ubiquitination / negative regulation of cell population proliferation / negative regulation of gene expression / ubiquitin protein ligase binding / regulation of DNA-templated transcription / negative regulation of apoptotic process / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / enzyme binding / endoplasmic reticulum / mitochondrion / proteolysis / nucleoplasm / nucleus / plasma membrane / cytosol / cytoplasm
Similarity search - Function
von Hippel-Lindau disease tumour suppressor, beta/alpha domain / von Hippel-Lindau disease tumour suppressor, alpha domain / von Hippel-Lindau disease tumour suppressor, beta domain / VHL superfamily / von Hippel-Lindau disease tumour suppressor, alpha domain superfamily / von Hippel-Lindau disease tumour suppressor, beta domain superfamily / VHL beta domain / VHL box domain / Elongin B / Ubiquitin family ...von Hippel-Lindau disease tumour suppressor, beta/alpha domain / von Hippel-Lindau disease tumour suppressor, alpha domain / von Hippel-Lindau disease tumour suppressor, beta domain / VHL superfamily / von Hippel-Lindau disease tumour suppressor, alpha domain superfamily / von Hippel-Lindau disease tumour suppressor, beta domain superfamily / VHL beta domain / VHL box domain / Elongin B / Ubiquitin family / Ubiquitin homologues / Ubiquitin domain profile. / Ubiquitin-like domain / Ubiquitin-like domain superfamily
Similarity search - Domain/homology
Chem-3JF / : / von Hippel-Lindau disease tumor suppressor / Isoform 2 of Elongin-C / Elongin-B
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.486 Å
AuthorsCollie, G.W.
Funding support1items
OrganizationGrant numberCountry
Other private
CitationJournal: Acs Med.Chem.Lett. / Year: 2025
Title: Discovery of a Series of Covalent Ligands That Bind to Cys77 of the Von Hippel-Lindau Tumor Suppressor Protein (VHL).
Authors: Lucas, S.C.C. / Xu, Y. / Hewitt, S. / Collie, G.W. / Fusani, L. / Kadamur, G. / Hadfield, T.E. / Su, N. / Truman, C. / Demanze, S. / Hao, H. / Phillips, C.
History
DepositionAug 19, 2024Deposition site: PDBE / Processing site: PDBE
Revision 1.0Mar 26, 2025Provider: repository / Type: Initial release
Revision 1.1Apr 30, 2025Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Elongin-B
B: Isoform 2 of Elongin-C
C: von Hippel-Lindau disease tumor suppressor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)42,2715
Polymers41,4323
Non-polymers8392
Water6,107339
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4960 Å2
ΔGint-38 kcal/mol
Surface area16310 Å2
MethodPISA
Unit cell
Length a, b, c (Å)74.781, 59.312, 97.316
Angle α, β, γ (deg.)90, 100.12, 90
Int Tables number5
Space group name H-MC121

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Components

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Protein , 3 types, 3 molecules ABC

#1: Protein Elongin-B / EloB / Elongin 18 kDa subunit / RNA polymerase II transcription factor SIII subunit B / SIII p18 / ...EloB / Elongin 18 kDa subunit / RNA polymerase II transcription factor SIII subunit B / SIII p18 / Transcription elongation factor B polypeptide 2


Mass: 12030.684 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ELOB, TCEB2 / Production host: Escherichia coli (E. coli) / References: UniProt: Q15370
#2: Protein Isoform 2 of Elongin-C / EloC / Elongin 15 kDa subunit / RNA polymerase II transcription factor SIII subunit C / SIII p15 / ...EloC / Elongin 15 kDa subunit / RNA polymerase II transcription factor SIII subunit C / SIII p15 / Transcription elongation factor B polypeptide 1


Mass: 10843.420 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ELOC, TCEB1 / Production host: Escherichia coli (E. coli) / References: UniProt: Q15369-2
#3: Protein von Hippel-Lindau disease tumor suppressor / Protein G7 / pVHL


Mass: 18558.162 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: VHL / Production host: Escherichia coli (E. coli) / References: UniProt: P40337

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Non-polymers , 3 types, 341 molecules

#4: Chemical ChemComp-3JF / N-acetyl-3-methyl-L-valyl-(4R)-4-hydroxy-N-[4-(4-methyl-1,3-thiazol-5-yl)benzyl]-L-prolinamide


Mass: 472.600 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C24H32N4O4S
#5: Chemical ChemComp-A1IMD / N-[2-(dimethylsulfamoyl)-5-[(1-methylpyrazol-4-yl)methoxy]phenyl]propanamide


Mass: 366.435 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C16H22N4O4S
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 339 / Source method: isolated from a natural source / Formula: H2O

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Details

Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.56 Å3/Da / Density % sol: 52.03 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / Details: 12 % PEG8000 and 100 mM MES buffer (pH 6.5).

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I04 / Wavelength: 0.95373 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Nov 30, 2023
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.95373 Å / Relative weight: 1
ReflectionResolution: 1.486→47.9 Å / Num. obs: 47660 / % possible obs: 92.1 % / Redundancy: 3.7 % / CC1/2: 0.987 / Net I/σ(I): 8.7
Reflection shellResolution: 1.486→1.622 Å / Num. unique obs: 2383 / CC1/2: 0.709

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Processing

Software
NameVersionClassification
BUSTER2.11.8refinement
autoPROCdata reduction
autoPROCdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.486→29.66 Å / Cor.coef. Fo:Fc: 0.945 / Cor.coef. Fo:Fc free: 0.929 / SU R Cruickshank DPI: 0.1 / Cross valid method: THROUGHOUT / SU R Blow DPI: 0.105 / SU Rfree Blow DPI: 0.102 / SU Rfree Cruickshank DPI: 0.099
RfactorNum. reflection% reflectionSelection details
Rfree0.2334 2369 -RANDOM
Rwork0.2039 ---
obs0.2054 47650 69 %-
Displacement parametersBiso mean: 22.02 Å2
Baniso -1Baniso -2Baniso -3
1-0.4085 Å20 Å2-0.4468 Å2
2---0.2965 Å20 Å2
3----0.1119 Å2
Refine analyzeLuzzati coordinate error obs: 0.23 Å
Refinement stepCycle: LAST / Resolution: 1.486→29.66 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms2639 0 58 339 3036
Refine LS restraints
Refine-IDTypeDev idealNumberRestraint functionWeight
X-RAY DIFFRACTIONt_bond_d0.012767HARMONIC2
X-RAY DIFFRACTIONt_angle_deg1.033766HARMONIC2
X-RAY DIFFRACTIONt_dihedral_angle_d953SINUSOIDAL2
X-RAY DIFFRACTIONt_gen_planes461HARMONIC5
X-RAY DIFFRACTIONt_it2767HARMONIC10
X-RAY DIFFRACTIONt_chiral_improper_torsion362SEMIHARMONIC5
X-RAY DIFFRACTIONt_ideal_dist_contact2673SEMIHARMONIC4
X-RAY DIFFRACTIONt_omega_torsion4.18
X-RAY DIFFRACTIONt_other_torsion15.54
LS refinement shellResolution: 1.49→1.57 Å
RfactorNum. reflection% reflection
Rfree0.2385 47 -
Rwork0.255 --
obs0.2542 953 9.58 %

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