PDB-9g48: Staphylococcus aureus MazF in complex with Nanobody 6

ID or keywords:

Entry

Database: PDB / ID: 9g48

Title

Staphylococcus aureus MazF in complex with Nanobody 6

Components

Endoribonuclease MazF
Nanobody 6

Keywords

TOXIN / Toxin-antitoxin system / Ribonuclease / Nanobody

Function / homology

Function and homology information

rRNA catabolic process / mRNA catabolic process / RNA endonuclease activity / Hydrolases; Acting on ester bonds / DNA binding / RNA binding
Similarity search - Function

Biological species

Staphylococcus aureus (bacteria)

Lama glama (llama)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 2.14353600683 Å

Authors

Zorzini, V. / Haesaerts, S. / Loris, R.

Funding support

Belgium, 1items

Citation

Journal: To Be Published
Title: Nanobody-mediated activation and inhibition of Staphylococcus aureus MazF
Authors: Prolic-Kalinsek, M. / Zorzini, V. / Haesaerts, S. / De Bruyn, P. / Loris, R.

History

Deposition	Jul 15, 2024	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Jul 24, 2024	Provider: repository / Type: Initial release

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	9g48.cif.gz	248.6 KB	Display	PDBx/mmCIF format
PDB format	pdb9g48.ent.gz	165.5 KB	Display	PDB format
PDBx/mmJSON format	9g48.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Summary document	9g48_validation.pdf.gz	479.5 KB	Display	wwPDB validaton report
Full document	9g48_full_validation.pdf.gz	484.1 KB	Display
Data in XML	9g48_validation.xml.gz	21.7 KB	Display
Data in CIF	9g48_validation.cif.gz	29.8 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/g4/9g48 ftp://data.pdbj.org/pub/pdb/validation_reports/g4/9g48	HTTPS FTP

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Related structure data

Related structure data	9g1yC 9g2gC 9g4gC 9g5lC C: citing same article (ref.)
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#136 - Apr 2011 Nanobodies similarity (1) #21 - Sep 2001 Antibodies similarity (1) #256 - Apr 2021 SARS-CoV-2 Spike and Antibodies similarity (1)

Deposited unit

A: Endoribonuclease MazF

B: Endoribonuclease MazF

C: Nanobody 6

D: Nanobody 6

hetero molecules

61.2 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author&software

Unit cell

Length a, b, c (Å)	60.140, 60.140, 246.240
Angle α, β, γ (deg.)	90.000, 90.000, 120.000
Int Tables number	152
Space group name H-M	P3₁21
Space group name Hall	P3₁2"
Symmetry operation	#1: x,y,z #2: -y,x-y,z+1/3 #3: -x+y,-x,z+2/3 #4: x-y,-y,-z+2/3 #5: -x,-x+y,-z+1/3 #6: y,x,-z

Components on special symmetry positions

ID	Model	Components
1	1	D-322- HOH
2	1	D-338- HOH

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Protein / Antibody , 2 types, 4 molecules ABCD

#1: Protein	Endoribonuclease MazF / SaMazF / Toxin MazF / mRNA interferase MazF Mass: 14952.206 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Staphylococcus aureus (bacteria) / Gene: mazF, SA1873 / Production host: Escherichia coli BL21(DE3) (bacteria) References: UniProt: Q7A4G9, Hydrolases; Acting on ester bonds
#2: Antibody	Nanobody 6 Mass: 15232.658 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Lama glama (llama) / Production host: Escherichia coli BL21(DE3) (bacteria)

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Non-polymers , 4 types, 188 molecules

#3: Chemical	ChemComp-CD / CADMIUM ION Mass: 112.411 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: Cd
#4: Chemical	ChemComp-PGE / TRIETHYLENE GLYCOL Mass: 150.173 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C₆H₁₄O₄
#5: Chemical	ChemComp-PG0 / 2-(2-METHOXYETHOXY)ETHANOL / PEG 6000 Mass: 120.147 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C₅H₁₂O₃ / Comment: inhibitor, precipitant*YM
#6: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 181 / Source method: isolated from a natural source / Formula: H₂O

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Details

Has ligand of interest	N

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.13 Å³/Da / Density % sol: 42.24 %
Crystal grow	Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 0.1 M CdCl2, 0.1 M Na-acetate pH 4.6, 30% (w/v) PEG-400 PH range: 4.6

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Data collection

Diffraction	Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: SOLEIL / Beamline: PROXIMA 2 / Wavelength: 0.978 Å
Detector	Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Feb 25, 2022
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.978 Å / Relative weight: 1
Reflection	Resolution: 2.14353→39.7614 Å / Num. obs: 28443 / % possible obs: 96.8 % / Redundancy: 2.845 % / B_iso Wilson estimate: 48.2159587816 Å² / CC_1/2: 0.995 / Net I/σ(I): 6.6
Reflection shell	Resolution: 2.14353→2.28 Å / Num. unique obs: 4525 / CC_1/2: 0.463 / % possible all: 82.9

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Processing

Software

Name	Version	Classification
PHENIX	1.11.1_2575	refinement
XDS		data reduction
XDS		data scaling
PHASER		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT / Resolution: 2.14353600683→39.7613289734 Å / SU ML: 0.333261603839 / Cross valid method: FREE R-VALUE / σ(F): 1.33786595283 / Phase error: 25.8905696562
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2

	R_factor	Num. reflection	% reflection
R_free	0.245384553422	1424	5.00650423654 %
R_work	0.216028462864	27019	-
obs	0.217533490853	28443	96.4398331808 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso mean: 59.5208165254 Å²

Refinement step

Cycle: LAST / Resolution: 2.14353600683→39.7613289734 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	3625	0	22	181	3828

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.00334216402996	3756
X-RAY DIFFRACTION	f_angle_d	0.539864812529	5089
X-RAY DIFFRACTION	f_chiral_restr	0.0447988882359	580
X-RAY DIFFRACTION	f_plane_restr	0.00442105461867	656
X-RAY DIFFRACTION	f_dihedral_angle_d	11.3334829017	2242

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.14354-2.2201	0.383973749353	140	0.336494865482	2646	X-RAY DIFFRACTION	96.035849707
2.2201-2.309	0.331768622531	143	0.304264546519	2704	X-RAY DIFFRACTION	98.2401656315
2.309-2.4141	0.31071585634	141	0.280493431779	2683	X-RAY DIFFRACTION	97.8516978517
2.4141-2.5414	0.279335299561	142	0.263843913788	2700	X-RAY DIFFRACTION	99.058905542
2.5414-2.7005	0.315846033609	145	0.239869010279	2760	X-RAY DIFFRACTION	99.3162393162
2.7005-2.909	0.254734817998	144	0.227452804977	2729	X-RAY DIFFRACTION	98.6607142857
2.909-3.2016	0.229580979734	143	0.207960026273	2716	X-RAY DIFFRACTION	97.3773841962
3.2016-3.6646	0.250397549125	140	0.191607245644	2666	X-RAY DIFFRACTION	94.861392833
3.6646-4.6159	0.194625603102	143	0.169829234642	2720	X-RAY DIFFRACTION	94.6759259259
4.6159-39.7613	0.242394896065	143	0.229246102077	2695	X-RAY DIFFRACTION	89.2452830189

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	3.10567718148	1.37915369311	3.91566280693	0.629707658939	1.52098943823	4.93934211266	-0.359179334137	-0.285509127352	-0.207240989068	0.507322985119	-0.257688069126	1.52319232565	0.902934913624	-1.20565853119	0.0570686880869	0.530761919776	-0.224414676105	0.177978271872	0.643989501622	-0.0751827841412	0.791001230353	7.52296352109	0.462686277161	20.5281605417
2	6.39280281083	-0.20792594236	-0.410394709034	6.15412155234	-0.950197777491	8.45460500245	0.090213836478	0.0980515985089	-0.371644459145	1.15361452537	-0.436508531116	0.568218395457	-0.655236083272	-0.463990184417	0.278193658402	0.58152971936	-0.051502936104	0.138744561291	0.443709894071	-0.121042058303	0.545096525577	10.876145183	3.57717987428	11.9091535239
3	3.53746323747	0.683491200841	2.93866629427	3.68522378687	2.27570857618	3.09882822722	-0.773749854487	0.455099889172	-0.886625367229	1.44557657365	-0.080892203723	0.750669707463	0.0929903869608	0.954976883138	0.517689165709	1.09174784567	-0.171536854646	0.197673927634	0.875166841026	0.0540086366014	0.914604693942	13.8905844419	17.6823397631	7.01974947702
4	2.83056945344	-1.96465551226	0.973678803202	2.03568119256	0.693501317247	2.34331789411	0.120688246745	0.0750889597804	-0.663965683484	-0.471058844414	-0.38504210169	-0.120357403138	-0.714124120835	0.489398638743	0.206189113978	0.513274035109	-0.0721305447994	0.086156098377	0.424821319809	-0.0209431423972	0.401754692054	12.7720170459	7.36860808694	16.1226651491
5	1.70671256504	-0.789083147079	-0.637875498192	2.77271981594	1.24958909904	2.9444907575	0.0481661595989	0.126676002185	-0.159862241792	-0.206536209096	0.0501790244075	-0.0564497406036	-0.0951726820851	0.00576585103071	0.00953745647325	0.441418857886	-0.0985892581558	0.00196652752277	0.327595892684	-0.00815461734996	0.286831416523	15.7397976441	8.98769380574	17.8293689904
6	0.320952990365	-0.204893545647	0.615483338127	0.347158682348	-0.327947649701	1.47291120306	0.102851848259	-0.202165680664	-0.125438443877	-0.90135100177	0.264188806571	1.04014148439	0.324892062314	0.271068750279	-0.149552498773	0.646278287032	-0.116764167721	-0.0781105023444	0.448410349831	0.0746252512587	0.636851647614	26.0499379864	1.10786301864	14.2232123384
7	1.50031294556	-0.268761195376	-1.86611399752	0.942484311624	1.98860880928	1.41846587236	-0.272735972847	0.627740665129	0.381927835323	-0.299543235471	0.388257352672	-0.266626845056	0.101512327352	-0.136986369451	-0.0994566104716	0.473943652623	-0.126039659745	0.0693420141298	0.460703256379	0.0125471694749	0.348891408333	15.9098063318	8.79521740267	12.9404193251
8	3.22389600524	-1.59948101529	-0.288127955763	4.66630888311	-0.310335126414	3.08284447153	-0.0700220547145	0.0601126396048	0.0867220838545	-0.524227511987	-0.422139713033	-0.192361474327	0.952715557379	-0.701218033309	0.533811370108	0.617826285975	-0.210319669626	0.0520956990793	0.446313663457	-0.0315809342266	0.576926296695	13.4227554421	-1.38211108507	14.5017998359
9	3.19921533294	-0.479188920197	1.17337041432	2.60279870541	2.00147715698	2.11643987117	0.194151182717	-0.608515494346	0.0409506131505	0.586035574369	-0.20890002193	-1.47287817181	0.251588536412	0.2457861996	-0.0563909201633	0.455273584653	-0.126489411539	0.0125522814058	0.500331878436	0.0518145329866	0.422257700725	22.5895850865	6.98146931732	25.5505696079
10	9.74824552621	-2.24251741201	-3.48219917936	2.8663815089	2.43871428705	2.35482496256	-0.544048090591	-1.34417470327	0.619625548543	1.39975285289	-0.760067496393	-0.968635794095	-0.267026862184	0.984014840793	-0.0746016271167	0.855613989508	-0.17909234632	-0.0181469580811	0.711187087952	0.0161843675073	0.564672468725	20.7939244766	18.3734997747	30.4675534175
11	5.76084466436	-1.75337189001	2.46462156466	2.5560889777	-0.0754688054128	6.18626196222	-0.314424617166	-0.010559729384	0.529938374049	0.221142660212	0.0995598830831	0.129437069496	-1.37159615405	0.514917849097	0.134866962042	0.650424260461	-0.069959156887	0.0358068302667	0.464538056067	0.029755742151	0.47451146795	15.3585502708	31.2730907495	22.6257933827
12	6.74679408681	1.78457366036	-1.09726087751	6.90915659972	-1.90615894456	0.56844888373	0.28991699674	2.15395308124	0.311944889381	-1.05855158218	-0.274336301755	0.451242721788	1.3130552533	-2.81227078562	-0.319979356087	1.22952825977	-0.182338302789	0.00812726815314	1.16782103973	0.00125091794545	0.897092940512	3.54074182209	23.03966272	12.9827314574
13	3.97863448026	-0.0665466817592	-1.60110177586	1.66146272675	1.40462899794	1.02781860059	0.113617178767	0.0978696587045	0.251485604386	-0.15378140074	-0.0177102586355	-0.0389238383689	-0.326044199472	-0.031390294449	0.0490442197234	0.40880364344	-0.0489851548463	0.0320651696511	0.446496862855	0.0639650163897	0.366453798351	11.7283605847	23.8212302077	23.4741757947
14	3.58946799431	0.659762483333	2.37252139928	0.997884486825	1.43712827696	4.07047448693	-0.804851120427	-0.316540002833	0.824512811632	0.746574503857	-0.00937047898911	0.584651076206	-0.606133360993	0.127608108147	0.434172090172	0.679702684015	0.031623438005	-0.0186131395404	0.463900540731	-0.0237494238052	0.687385376072	1.98115864298	31.0954005385	31.5417623615
15	4.83237770895	0.703044377016	-2.1325150733	0.97791483603	1.01387776854	2.94422131622	-0.258678462296	0.699711400487	-0.571825860706	-0.568883258245	-0.165713947251	0.0695504221719	0.0193819324859	-0.501581361906	0.425574974836	0.56126477534	-0.0788451659667	0.0220743384925	0.373355714109	-0.0206924612224	0.384155941281	9.62026618903	22.6380750305	19.370985653
16	2.38627813878	-0.349286157575	1.10095712612	3.07234997542	-0.69315104813	0.133336683759	0.197787207406	-0.348608554254	0.486870860158	0.486253640525	-0.328879334843	-0.225890895257	-0.245230984698	0.0862642932987	0.127309266087	0.435525514169	-0.0287833052413	0.0809280104686	0.415491518026	0.0263838950218	0.435781265865	12.2261025357	25.8117622622	29.9808861699
17	0.110847004643	0.298067774235	-0.529288239001	2.12684715692	-1.30360020663	1.68714046319	-0.144929816808	-0.496495830051	-1.17512855358	0.84335886969	-0.746653669811	0.156375812532	0.45501907056	0.932995517219	0.71175696028	0.460357238332	-0.073234861957	0.139122065477	0.671855806395	0.0397574007177	0.579457115443	14.4614190384	7.51819845291	32.1732880874
18	1.87564441942	1.67875383042	0.240862916694	3.29105387637	2.26662775243	2.42732432192	-0.123651356924	0.469809379064	0.153931516107	-0.571731622143	0.371244105841	-0.496720919201	-0.481474700518	0.346916404747	-0.135390336921	0.467357699857	-0.085602424563	0.0600883270586	0.479691539249	-0.0104573842403	0.540019260112	51.0567352043	31.8058711238	11.9701450698
19	3.72380332863	0.486698090491	-1.47940591112	3.7355542195	0.479322731902	2.04011190707	-0.0817091467476	-0.253150652533	-0.0889502394365	0.534626208606	-0.0157358087801	0.0748180103347	0.431248186809	-0.155309771027	-0.0338390506318	0.678136830772	-0.100574597371	0.0794457634358	0.497417235046	0.0191804341602	0.545480625945	41.5037606713	24.3324055033	13.0050928597
20	4.29050703554	-0.358802195048	-3.44914591437	1.52228873542	-0.258217925063	2.90144962075	0.268741818694	0.783292681854	1.07502412879	0.197410553209	-0.412334819254	0.996215717377	0.766515967703	-1.65796833196	0.564921891983	0.561295065105	-0.038327277771	0.0630346443318	0.683472214222	-0.00537668737313	0.705377118573	32.6992907457	29.4580249837	6.86312031415
21	3.66927003783	2.7401744401	-0.00407571800196	7.84269401029	2.98082618478	6.21770844448	-0.265854430265	0.288144677906	0.417669947218	0.0250845578252	0.13602458056	0.151033001958	0.290132086215	-0.526163029084	0.254453411482	0.455866117915	-0.0822746139735	0.0588214375132	0.360294154024	-0.0123225569066	0.40323710558	42.0372462272	32.7492039523	7.71993411283
22	2.16974479546	2.50520295633	0.215973269093	1.32445020223	-0.346618348307	0.721486894921	0.276848630452	0.0573293877229	0.0862985699341	0.326804646732	-0.20833107357	0.175682397859	0.00935280832294	-0.258807871232	0.0630912331742	0.530045285024	-0.0970264150744	0.108685737174	0.536101749085	-0.0384552793881	0.461355259893	38.4044281059	26.2415130118	13.4883337421
23	1.81270498133	1.15737967587	0.673496199934	4.91448063023	0.909999857031	2.29970951363	0.148249728868	-0.244967338132	-0.624390889031	0.660945297359	-0.00802699538667	-0.49076112263	0.345109010059	0.1217879317	0.164690641117	0.559183581926	-0.0943890409083	0.0579493380825	0.525969710948	-0.0102347945139	0.502531995216	43.9890446645	22.2881056497	16.359990204
24	0.343430215533	0.884838913853	0.682716728866	1.0974536043	0.361793346457	1.19573226707	-0.144725682208	1.21920476584	0.448130530821	0.309235676823	-0.126216561569	1.95874030164	-0.206726711586	-1.11045541892	0.0399037126754	0.559558493817	-0.0610003119786	0.124733336811	0.524834090871	-0.0198432150667	0.61249560554	-20.530115848	2.0404749777	39.9437786923
25	0.705828895968	-0.108978646182	-0.884966271767	0.840946769767	0.0493291974753	2.11435394347	-0.225800211695	0.221986857156	0.13445417362	-0.107222294481	0.181676401537	0.0322605586428	-0.178865388253	-0.125042434132	0.0671677101336	0.419146092776	-0.0897051038726	-0.0175347394853	0.493334962641	0.0387526475073	0.499040589081	-14.4581703307	3.84400088368	33.7313785727
26	0.584442263001	0.0850832078604	-0.632161592226	2.14400405833	1.58610107771	1.90371688253	-0.27369744314	0.688443148732	0.56697121069	-0.493834340475	-0.423551917028	-0.0208882283631	-1.91490029691	0.575950908103	0.403033398543	0.947091095235	-0.189897074298	-0.120410643734	0.639198727219	0.12036168457	0.567386331066	-12.3671486226	6.46907181199	21.8522106764
27	3.18947374343	0.203023778169	-1.60070598081	5.66952261279	0.991846079052	2.95355316012	0.011925763163	0.726050776476	-0.00466306439695	-0.613492622565	-0.139150641089	0.257705110272	0.17731988819	-0.568648790233	0.030953227551	0.466238846114	-0.105450279319	0.00143665130853	0.645833488855	-0.0435196664719	0.476703571367	-18.6804069324	1.345140571	27.7969505492
28	1.89199548097	2.41205139628	-0.413475039715	1.88273617424	-1.28085595724	-0.652191912495	0.00821569945527	0.133330480182	0.012888589793	-0.190464680654	-0.0520405771683	0.263325623629	0.148965177926	0.030177088046	0.0544801984725	0.446896809185	-0.0659147975033	0.018397360681	0.495282995153	0.00889715442771	0.447898616307	-10.0750542343	7.15925262689	32.6814145403
29	3.99204767586	1.23457680762	2.04769634516	2.74179521311	-1.31237754633	2.84472377772	0.576856402613	-0.300913124982	-2.21452484993	0.563820170218	-0.783403140936	0.284172411031	1.14860655622	0.194441997362	0.252129836865	0.6527211704	-0.100922872828	0.0762266606772	0.511829276039	0.0309363606141	0.762958139373	-15.9298946238	-10.8170103211	42.2754957718

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid -2 through 5 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 6 through 11 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 12 through 22 )
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 23 through 31 )
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 32 through 57 )
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 58 through 66 )
7	X-RAY DIFFRACTION	7	chain 'A' and (resid 67 through 86 )
8	X-RAY DIFFRACTION	8	chain 'A' and (resid 87 through 95 )
9	X-RAY DIFFRACTION	9	chain 'A' and (resid 96 through 109 )
10	X-RAY DIFFRACTION	10	chain 'A' and (resid 110 through 114 )
11	X-RAY DIFFRACTION	11	chain 'B' and (resid 0 through 10 )
12	X-RAY DIFFRACTION	12	chain 'B' and (resid 11 through 23 )
13	X-RAY DIFFRACTION	13	chain 'B' and (resid 24 through 57 )
14	X-RAY DIFFRACTION	14	chain 'B' and (resid 58 through 71 )
15	X-RAY DIFFRACTION	15	chain 'B' and (resid 72 through 86 )
16	X-RAY DIFFRACTION	16	chain 'B' and (resid 87 through 109 )
17	X-RAY DIFFRACTION	17	chain 'B' and (resid 110 through 116 )
18	X-RAY DIFFRACTION	18	chain 'C' and (resid 3 through 24 )
19	X-RAY DIFFRACTION	19	chain 'C' and (resid 25 through 51 )
20	X-RAY DIFFRACTION	20	chain 'C' and (resid 52 through 60 )
21	X-RAY DIFFRACTION	21	chain 'C' and (resid 61 through 83 )
22	X-RAY DIFFRACTION	22	chain 'C' and (resid 84 through 112 )
23	X-RAY DIFFRACTION	23	chain 'C' and (resid 113 through 131 )
24	X-RAY DIFFRACTION	24	chain 'D' and (resid 3 through 12 )
25	X-RAY DIFFRACTION	25	chain 'D' and (resid 13 through 51 )
26	X-RAY DIFFRACTION	26	chain 'D' and (resid 52 through 60 )
27	X-RAY DIFFRACTION	27	chain 'D' and (resid 61 through 83 )
28	X-RAY DIFFRACTION	28	chain 'D' and (resid 84 through 124 )
29	X-RAY DIFFRACTION	29	chain 'D' and (resid 125 through 133 )

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