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データを開く
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基本情報
| 登録情報 | データベース: PDB / ID: 9g33 | ||||||
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| タイトル | Stalled 90S - Utp23-Krr1-deltaC3 | ||||||
要素 |
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キーワード | RIBOSOME / 90S / pre-ribosome / snoRNA / snR30 / snoRNP / ribosome biogenesis / H/ACA / 18S rRNA | ||||||
| 機能・相同性 | 機能・相同性情報tRNA wobble cytosine modification / tRNA cytidine N4-acetyltransferase activity / rRNA acetylation involved in maturation of SSU-rRNA / 18S rRNA cytidine N-acetyltransferase activity / tRNA acetylation / RNA fragment catabolic process / rRNA small subunit pseudouridine methyltransferase Nep1 / rRNA 2'-O-methylation / Noc4p-Nop14p complex / t-UTP complex ...tRNA wobble cytosine modification / tRNA cytidine N4-acetyltransferase activity / rRNA acetylation involved in maturation of SSU-rRNA / 18S rRNA cytidine N-acetyltransferase activity / tRNA acetylation / RNA fragment catabolic process / rRNA small subunit pseudouridine methyltransferase Nep1 / rRNA 2'-O-methylation / Noc4p-Nop14p complex / t-UTP complex / Pwp2p-containing subcomplex of 90S preribosome / Mpp10 complex / nuclear microtubule / rRNA (pseudouridine) methyltransferase activity / snoRNA guided rRNA 2'-O-methylation / rRNA modification / histone H2AQ104 methyltransferase activity / septum digestion after cytokinesis / tRNA re-export from nucleus / snRNA binding / box C/D sno(s)RNA 3'-end processing / rRNA methyltransferase activity / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / regulation of rRNA processing / regulation of transcription by RNA polymerase I / RNA folding chaperone / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / box C/D methylation guide snoRNP complex / rDNA heterochromatin / U4/U6 snRNP / positive regulation of rRNA processing / tRNA export from nucleus / single-stranded telomeric DNA binding / rRNA primary transcript binding / sno(s)RNA-containing ribonucleoprotein complex / O-methyltransferase activity / small nuclear ribonucleoprotein complex / rRNA base methylation / protein localization to nucleolus / SUMOylation of RNA binding proteins / U4 snRNA binding / rRNA methylation / mTORC1-mediated signalling / Protein hydroxylation / U4 snRNP / mRNA modification / U3 snoRNA binding / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / poly(A)+ mRNA export from nucleus / snoRNA binding / preribosome, small subunit precursor / precatalytic spliceosome / Major pathway of rRNA processing in the nucleolus and cytosol / establishment of cell polarity / SRP-dependent cotranslational protein targeting to membrane / positive regulation of transcription by RNA polymerase I / GTP hydrolysis and joining of the 60S ribosomal subunit / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / spliceosomal complex assembly / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / L13a-mediated translational silencing of Ceruloplasmin expression / 90S preribosome / proteasome assembly / RNA processing / ribosomal subunit export from nucleus / U4/U6 x U5 tri-snRNP complex / regulation of translational fidelity / vesicle-mediated transport / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal small subunit export from nucleus / RNA endonuclease activity / 転移酵素; アシル基を移すもの; アミノアシル基以外のアシル基を移すもの / nuclear periphery / 転移酵素; 一炭素原子の基を移すもの; メチル基を移すもの / ribosome assembly / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / spliceosomal complex / maturation of SSU-rRNA / small-subunit processome / translational initiation / mRNA splicing, via spliceosome / maintenance of translational fidelity / enzyme activator activity / rRNA processing / : / peroxisome / protein transport / ribosomal small subunit assembly / ribosome biogenesis / ribosomal small subunit biogenesis / small ribosomal subunit / cytosolic small ribosomal subunit / 加水分解酵素; 酸無水物に作用; GTPに作用・細胞または細胞小器官の運動に関与 / cytoplasmic translation / tRNA binding 類似検索 - 分子機能 | ||||||
| 生物種 | ![]() | ||||||
| 手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.05 Å | ||||||
データ登録者 | Thoms, M. / Berninghausen, O. / Beckmann, R. | ||||||
| 資金援助 | European Union, 1件
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引用 | ジャーナル: Nat Commun / 年: 2025タイトル: H/ACA snR30 snoRNP guides independent 18S rRNA subdomain formation. 著者: Paulina Fischer / Matthias Thoms / Benjamin Lau / Timo Denk / Maria Kuvshinova / Otto Berninghausen / Dirk Flemming / Ed Hurt / Roland Beckmann / ![]() 要旨: Ribosome biogenesis follows a cascade of pre-rRNA folding and processing steps, coordinated with ribosomal protein incorporation. Nucleolar 90S pre-ribosomes are well-described stable intermediates, ...Ribosome biogenesis follows a cascade of pre-rRNA folding and processing steps, coordinated with ribosomal protein incorporation. Nucleolar 90S pre-ribosomes are well-described stable intermediates, composed of pre-18S rRNA, ribosomal S-proteins, U3 snoRNA, and ~70 assembly factors. However, how numerous snoRNAs control pre-rRNA modification and folding during early maturation events remains unclear. We identify snR30 (human U17), the only essential H/ACA snoRNA in yeast, which binds with Cbf5-Gar1-Nop10-Nhp2 to a pre-18S rRNA subdomain containing platform helices and ES6 of the 40S central domain. Integration into the 90S is blocked by RNA hybridization with snR30. The snoRNP complex coordinates the recruitment of early assembly factors Krr1-Utp23-Kri1 and ribosomal proteins uS11-uS15, enabling isolated subdomain assembly. Krr1-dependent release of snR30 culminates in integration of the platform into the 90S. Our study reveals the essential role of snR30 in chaperoning central domain formation as a discrete assembly unit externalized from the pre-ribosomal core. #1: ジャーナル: Acta Crystallogr., Sect. D: Biol. Crystallogr.年: 2018 タイトル: Real-space refinement in PHENIX for cryo-EM and crystallography 著者: Adams, P.D. | ||||||
| 履歴 |
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構造の表示
| 構造ビューア | 分子: Molmil Jmol/JSmol |
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ダウンロードとリンク
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ダウンロード
| PDBx/mmCIF形式 | 9g33.cif.gz | 4.7 MB | 表示 | PDBx/mmCIF形式 |
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| PDB形式 | pdb9g33.ent.gz | 表示 | PDB形式 | |
| PDBx/mmJSON形式 | 9g33.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
| その他 | その他のダウンロード |
-検証レポート
| アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/g3/9g33 ftp://data.pdbj.org/pub/pdb/validation_reports/g3/9g33 | HTTPS FTP |
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-関連構造データ
| 関連構造データ | ![]() 50991MC ![]() 9g25C ![]() 9g28C C: 同じ文献を引用 ( M: このデータのモデリングに利用したマップデータ |
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| 類似構造データ | 類似検索 - 機能・相同性 F&H 検索 |
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リンク
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集合体
| 登録構造単位 | ![]()
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| 1 |
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要素
+RNA鎖 , 3種, 3分子 D3D2D4
+U3 small nucleolar ribonucleoprotein protein ... , 3種, 3分子 CJCIJE
+U3 small nucleolar RNA-associated protein ... , 17種, 17分子 CKUEUHUKULUMUOUPUNUFUGURUUUDUIUJUT
+Ribosome biogenesis protein ... , 3種, 3分子 CLJCUZ
+40S ribosomal protein ... , 13種, 13分子 DYDFDIDJDSDEDXDWDGDLDcDQDH
+RRNA-processing protein ... , 2種, 2分子 UXJM
+タンパク質 , 16種, 20分子 JGJFCACBUCCFCGJHJJJKJQCHJNJOCMJPUQUAJAJB
+Nucleolar complex protein ... , 2種, 2分子 UBUS
+Nucleolar protein ... , 2種, 2分子 CECD
+非ポリマー , 2種, 2分子 


+詳細
-実験情報
-実験
| 実験 | 手法: 電子顕微鏡法 |
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| EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
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試料調製
| 構成要素 | 名称: Utp23-FTpA, Krr1-deltaC3 / タイプ: RIBOSOME / Entity ID: #1-#61 / 由来: NATURAL |
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| 由来(天然) | 生物種: ![]() |
| 緩衝液 | pH: 7.5 |
| 試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
| 急速凍結 | 凍結剤: ETHANE |
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電子顕微鏡撮影
| 実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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| 顕微鏡 | モデル: TFS KRIOS |
| 電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: FLOOD BEAM |
| 電子レンズ | モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 3500 nm / 最小 デフォーカス(公称値): 500 nm |
| 撮影 | 電子線照射量: 46.4 e/Å2 フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) |
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解析
| EMソフトウェア | 名称: PHENIX / バージョン: 1.19.2_4158: / カテゴリ: モデル精密化 | ||||||||||||||||||||||||
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| CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
| 3次元再構成 | 解像度: 3.05 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 32278 / 対称性のタイプ: POINT | ||||||||||||||||||||||||
| 拘束条件 |
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FIELD EMISSION GUN