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- PDB-9g2e: Cryo-EM structure of the beta3 homomeric GABA(A) receptor in the ... -

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Basic information

Entry
Database: PDB / ID: 9g2e
TitleCryo-EM structure of the beta3 homomeric GABA(A) receptor in the long-lived desensitised state (C5) sprayed with PBS
ComponentsGamma-aminobutyric acid receptor subunit beta-3
KeywordsMEMBRANE PROTEIN / GABA / neurotransmission / gating cycle / time-resolved cryo-EM
Function / homology
Function and homology information


circadian sleep/wake cycle, REM sleep / reproductive behavior / hard palate development / cellular response to histamine / GABA receptor activation / inner ear receptor cell development / GABA-A receptor activity / GABA-gated chloride ion channel activity / GABA-A receptor complex / inhibitory synapse assembly ...circadian sleep/wake cycle, REM sleep / reproductive behavior / hard palate development / cellular response to histamine / GABA receptor activation / inner ear receptor cell development / GABA-A receptor activity / GABA-gated chloride ion channel activity / GABA-A receptor complex / inhibitory synapse assembly / innervation / response to anesthetic / postsynaptic specialization membrane / inhibitory postsynaptic potential / gamma-aminobutyric acid signaling pathway / synaptic transmission, GABAergic / cellular response to zinc ion / exploration behavior / motor behavior / roof of mouth development / cochlea development / Signaling by ERBB4 / social behavior / chloride channel complex / cytoplasmic vesicle membrane / chloride transmembrane transport / cerebellum development / learning / transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential / GABA-ergic synapse / memory / dendritic spine / postsynaptic membrane / response to xenobiotic stimulus / cell surface / signal transduction / identical protein binding / plasma membrane
Similarity search - Function
Gamma-aminobutyric-acid A receptor, beta subunit / Gamma-aminobutyric acid A receptor/Glycine receptor alpha / Neurotransmitter-gated ion-channel, conserved site / Neurotransmitter-gated ion-channels signature. / Neurotransmitter-gated ion-channel transmembrane domain / Neurotransmitter-gated ion-channel transmembrane region / Neurotransmitter-gated ion-channel transmembrane domain superfamily / Neuronal acetylcholine receptor / Neurotransmitter-gated ion-channel / Neurotransmitter-gated ion-channel ligand-binding domain ...Gamma-aminobutyric-acid A receptor, beta subunit / Gamma-aminobutyric acid A receptor/Glycine receptor alpha / Neurotransmitter-gated ion-channel, conserved site / Neurotransmitter-gated ion-channels signature. / Neurotransmitter-gated ion-channel transmembrane domain / Neurotransmitter-gated ion-channel transmembrane region / Neurotransmitter-gated ion-channel transmembrane domain superfamily / Neuronal acetylcholine receptor / Neurotransmitter-gated ion-channel / Neurotransmitter-gated ion-channel ligand-binding domain / Neurotransmitter-gated ion-channel ligand-binding domain superfamily / Neurotransmitter-gated ion-channel ligand binding domain
Similarity search - Domain/homology
DECANE / Gamma-aminobutyric acid receptor subunit beta-3
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.5 Å
AuthorsMihaylov, D.B. / Malinauskas, T. / Aricescu, A.R.
Funding support United Kingdom, United States, 6items
OrganizationGrant numberCountry
Medical Research Council (MRC, United Kingdom)MC_UP_1201/15 United Kingdom
Cancer Research UKDRCRPG-May23/100002 United Kingdom
Medical Research Council (MRC, United Kingdom)MR/L009609/1 United Kingdom
Medical Research Council (MRC, United Kingdom)MC_EX_MR/T046279/1 United Kingdom
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)1R01-GM135550 United States
National Science Foundation (NSF, United States)NeuroNex 2014862 United States
Citation
Journal: To Be Published
Title: Cryo-EM structure of the beta3 homomeric GABA(A) receptor in the long-lived desensitised state (C5) sprayed with PBS
Authors: Mihaylov, D.B. / Malinauskas, T. / Aricescu, A.R.
#1: Journal: Acta Crystallogr D Struct Biol / Year: 2019
Title: Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix.
Authors: Dorothee Liebschner / Pavel V Afonine / Matthew L Baker / Gábor Bunkóczi / Vincent B Chen / Tristan I Croll / Bradley Hintze / Li Wei Hung / Swati Jain / Airlie J McCoy / Nigel W Moriarty ...Authors: Dorothee Liebschner / Pavel V Afonine / Matthew L Baker / Gábor Bunkóczi / Vincent B Chen / Tristan I Croll / Bradley Hintze / Li Wei Hung / Swati Jain / Airlie J McCoy / Nigel W Moriarty / Robert D Oeffner / Billy K Poon / Michael G Prisant / Randy J Read / Jane S Richardson / David C Richardson / Massimo D Sammito / Oleg V Sobolev / Duncan H Stockwell / Thomas C Terwilliger / Alexandre G Urzhumtsev / Lizbeth L Videau / Christopher J Williams / Paul D Adams /
Abstract: Diffraction (X-ray, neutron and electron) and electron cryo-microscopy are powerful methods to determine three-dimensional macromolecular structures, which are required to understand biological ...Diffraction (X-ray, neutron and electron) and electron cryo-microscopy are powerful methods to determine three-dimensional macromolecular structures, which are required to understand biological processes and to develop new therapeutics against diseases. The overall structure-solution workflow is similar for these techniques, but nuances exist because the properties of the reduced experimental data are different. Software tools for structure determination should therefore be tailored for each method. Phenix is a comprehensive software package for macromolecular structure determination that handles data from any of these techniques. Tasks performed with Phenix include data-quality assessment, map improvement, model building, the validation/rebuilding/refinement cycle and deposition. Each tool caters to the type of experimental data. The design of Phenix emphasizes the automation of procedures, where possible, to minimize repetitive and time-consuming manual tasks, while default parameters are chosen to encourage best practice. A graphical user interface provides access to many command-line features of Phenix and streamlines the transition between programs, project tracking and re-running of previous tasks.
History
DepositionJul 10, 2024Deposition site: PDBE / Processing site: PDBE
Revision 1.0Jul 23, 2025Provider: repository / Type: Initial release
Revision 1.0Jul 23, 2025Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release
Revision 1.0Jul 23, 2025Data content type: Additional map / Part number: 1 / Data content type: Additional map / Provider: repository / Type: Initial release
Revision 1.0Jul 23, 2025Data content type: FSC / Data content type: FSC / Provider: repository / Type: Initial release
Revision 1.0Jul 23, 2025Data content type: Half map / Part number: 1 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0Jul 23, 2025Data content type: Half map / Part number: 2 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0Jul 23, 2025Data content type: Image / Data content type: Image / Provider: repository / Type: Initial release
Revision 1.0Jul 23, 2025Data content type: Mask / Part number: 1 / Data content type: Mask / Provider: repository / Type: Initial release
Revision 1.0Jul 23, 2025Data content type: Primary map / Data content type: Primary map / Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Gamma-aminobutyric acid receptor subunit beta-3
B: Gamma-aminobutyric acid receptor subunit beta-3
C: Gamma-aminobutyric acid receptor subunit beta-3
D: Gamma-aminobutyric acid receptor subunit beta-3
E: Gamma-aminobutyric acid receptor subunit beta-3
hetero molecules


Theoretical massNumber of molelcules
Total (without water)237,59835
Polymers228,2165
Non-polymers9,38230
Water8,197455
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1(chain "E" and (resid 4 through 40 or resid 42...
d_2ens_1(chain "B" and (resid 4 through 40 or resid 42...
d_3ens_1(chain "C" and (resid 4 through 40 or resid 42...
d_4ens_1(chain "D" and (resid 4 through 40 or resid 42...
d_5ens_1(chain "A" and (resid 4 through 40 or resid 42...
d_1ens_2chain "e"
d_2ens_2chain "b"
d_3ens_2chain "c"
d_4ens_2chain "d"
d_5ens_2chain "a"

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
d_11ens_1ASNASNMETMETEE4 - 4058 - 94
d_12ens_1ILEILEGLNGLNEE42 - 6596 - 119
d_13ens_1TRPTRPMETMETEE67 - 137121 - 191
d_14ens_1ASPASPASPASPEE139 - 163193 - 217
d_15ens_1GLUGLUILEILEEE165 - 242219 - 296
d_16ens_1TYRTYRSERSEREE244 - 436298 - 383
d_17ens_1PHEPHEVALVALEE438 - 447385 - 394
d_21ens_1ASNASNMETMETBB4 - 4058 - 94
d_22ens_1ILEILEGLNGLNBB42 - 6596 - 119
d_23ens_1TRPTRPMETMETBB67 - 137121 - 191
d_24ens_1ASPASPASPASPBB139 - 163193 - 217
d_25ens_1GLUGLUILEILEBB165 - 242219 - 296
d_26ens_1TYRTYRSERSERBB244 - 436298 - 383
d_27ens_1PHEPHEVALVALBB438 - 447385 - 394
d_31ens_1ASNASNMETMETCC4 - 4058 - 94
d_32ens_1ILEILEGLNGLNCC42 - 6596 - 119
d_33ens_1TRPTRPMETMETCC67 - 137121 - 191
d_34ens_1ASPASPASPASPCC139 - 163193 - 217
d_35ens_1GLUGLUILEILECC165 - 242219 - 296
d_36ens_1TYRTYRSERSERCC244 - 436298 - 383
d_37ens_1PHEPHEVALVALCC438 - 447385 - 394
d_41ens_1ASNASNMETMETDD4 - 4058 - 94
d_42ens_1ILEILEGLNGLNDD42 - 6596 - 119
d_43ens_1TRPTRPMETMETDD67 - 137121 - 191
d_44ens_1ASPASPASPASPDD139 - 163193 - 217
d_45ens_1GLUGLUILEILEDD165 - 242219 - 296
d_46ens_1TYRTYRSERSERDD244 - 436298 - 383
d_47ens_1PHEPHEVALVALDD438 - 447385 - 394
d_51ens_1ASNASNMETMETAA4 - 4058 - 94
d_52ens_1ILEILEGLNGLNAA42 - 6596 - 119
d_53ens_1TRPTRPMETMETAA67 - 137121 - 191
d_54ens_1ASPASPASPASPAA139 - 163193 - 217
d_55ens_1GLUGLUILEILEAA165 - 242219 - 296
d_56ens_1TYRTYRSERSERAA244 - 436298 - 383
d_57ens_1PHEPHEVALVALAA438 - 447385 - 394
d_11ens_2NAGNAGNAGNAGeN1
d_12ens_2NAGNAGNAGNAGeN2
d_13ens_2BMABMABMABMAeN3
d_14ens_2MANMANMANMANeN4
d_15ens_2MANMANMANMANeN5
d_16ens_2NAGNAGNAGNAGJO1
d_17ens_2NAGNAGNAGNAGJO2
d_18ens_2NAGNAGNAGNAGEIA504
d_21ens_2NAGNAGNAGNAGbH1
d_22ens_2NAGNAGNAGNAGbH2
d_23ens_2BMABMABMABMAbH3
d_24ens_2MANMANMANMANbH4
d_25ens_2MANMANMANMANbH5
d_26ens_2NAGNAGNAGNAGGI1
d_27ens_2NAGNAGNAGNAGGI2
d_28ens_2NAGNAGNAGNAGBV503
d_31ens_2NAGNAGNAGNAGcJ1
d_32ens_2NAGNAGNAGNAGcJ2
d_33ens_2BMABMABMABMAcJ3
d_34ens_2MANMANMANMANcJ4
d_35ens_2MANMANMANMANcJ5
d_36ens_2NAGNAGNAGNAGHK1
d_37ens_2NAGNAGNAGNAGHK2
d_38ens_2NAGNAGNAGNAGCZ503
d_41ens_2NAGNAGNAGNAGdL1
d_42ens_2NAGNAGNAGNAGdL2
d_43ens_2BMABMABMABMAdL3
d_44ens_2MANMANMANMANdL4
d_45ens_2MANMANMANMANdL5
d_46ens_2NAGNAGNAGNAGIM1
d_47ens_2NAGNAGNAGNAGIM2
d_48ens_2NAGNAGNAGNAGDEA504
d_51ens_2NAGNAGNAGNAGaF1
d_52ens_2NAGNAGNAGNAGaF2
d_53ens_2BMABMABMABMAaF3
d_54ens_2MANMANMANMANaF4
d_55ens_2MANMANMANMANaF5
d_56ens_2NAGNAGNAGNAGFG1
d_57ens_2NAGNAGNAGNAGFG2
d_58ens_2NAGNAGNAGNAGAP501

NCS ensembles :
ID
ens_1
ens_2

NCS oper:
IDCodeMatrixVector
1given(-0.808233202853, 0.588862536718, 5.15468128835E-5), (-0.588862534309, -0.808233204372, 5.51177227221E-5), (7.41186077722E-5, 1.41939866995E-5, 0.999999997152)220.059488682, 432.527168912, -0.0128254122211
2given(-0.809619099364, -0.586955631327, 2.82511130886E-5), (0.586955613486, -0.809619060789, 0.000290179576423), (-0.000147449896832, 0.000251517076732, 0.999999957499)432.459020621, 220.555018887, -0.0217192115863
3given(0.30857369668, -0.951200360531, 0.000384504289886), (0.951200403021, 0.308573550035, -0.00039687653685), (0.000258861251204, 0.000488206295604, 0.999999847323)296.341956031, -46.8073841113, -0.135867036871
4given(0.309776077843, 0.950809539927, -1.95049991275E-5), (-0.950809509199, 0.309776062469, -0.000261413103963), (-0.000242511891282, 9.95250646899E-5, 0.999999965641)-47.0050559956, 296.183022137, 0.0169688053684
5given(-0.808115580693, 0.589023941464, 6.79956499459E-5), (-0.589023944998, -0.808115570568, -0.000129712158023), (-2.14552231223E-5, -0.000144873481877, 0.999999989276)220.011063669, 432.557772881, 0.0313084300916
6given(-0.809504854403, -0.587113183882, 3.13246275164E-6), (0.587113176562, -0.809504843446, 0.000162311764334), (-9.27596329702E-5, 0.000133231271312, 0.999999986823)432.475868928, 220.525740554, -0.0104763205774
7given(0.308485799565, -0.951228894551, 0.000318806496996), (0.9512288776, 0.308485630494, -0.000488056256713), (0.000365905990321, 0.000453816370893, 0.999999830082)296.370298001, -46.7837098154, -0.146790339729
8given(0.309229214644, 0.950987522331, 0.000158689498134), (-0.950987505041, 0.309229242694, -0.000201788651674), (-0.000240969923221, -8.85127836255E-5, 0.999999967049)-46.975054402, 296.300375931, 0.05318051926

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Components

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Protein , 1 types, 5 molecules ABCDE

#1: Protein
Gamma-aminobutyric acid receptor subunit beta-3 / GABA(A) receptor subunit beta-3 / GABAAR subunit beta-3


Mass: 45643.246 Da / Num. of mol.: 5
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: GABRB3 / Plasmid: pHLsec / Cell line (production host): EXPI 293F / Production host: Homo sapiens (human) / References: UniProt: P28472

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Sugars , 3 types, 15 molecules

#2: Polysaccharide
alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2- ...alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 910.823 Da / Num. of mol.: 5
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-3[DManpa1-6]DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/3,5,4/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3-3/a4-b1_b4-c1_c3-d1_c6-e1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{}[(6+1)][a-D-Manp]{}}}}}LINUCSPDB-CARE
#3: Polysaccharide
2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 424.401 Da / Num. of mol.: 5
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/1,2,1/[a2122h-1b_1-5_2*NCC/3=O]/1-1/a4-b1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}}}LINUCSPDB-CARE
#4: Sugar
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 3 types, 470 molecules

#5: Chemical
ChemComp-D10 / DECANE


Mass: 142.282 Da / Num. of mol.: 10 / Source method: obtained synthetically / Formula: C10H22
#6: Chemical
ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: Cl
#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 455 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Cryo-EM structure of the beta3 homomeric GABA(A) receptor in the long-lived desensitised state (C5) sprayed with PBS
Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT
Molecular weightValue: 0.25 MDa / Experimental value: NO
Source (natural)Organism: Homo sapiens (human)
Source (recombinant)Organism: Homo sapiens (human) / Cell: EXPI 293F / Plasmid: pHLsec
Buffer solutionpH: 7.4 / Details: 137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
Buffer component
IDConc.NameFormulaBuffer-ID
1137 mMsodium chlorideNaCl1
22.7 mMpotassium chlorideKCl1
34.3 mMDisodium phosphateNa2HPO1
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportDetails: current: 30mA / Grid material: GOLD / Grid type: Quantifoil R1.2/1.3
VitrificationInstrument: HOMEMADE PLUNGER / Cryogen name: ETHANE / Humidity: 95 % / Chamber temperature: 281 K

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal magnification: 96000 X / Calibrated magnification: 96000 X / Nominal defocus max: 2000 nm / Nominal defocus min: 1000 nm / Cs: 2.7 mm / C2 aperture diameter: 50 µm / Alignment procedure: COMA FREE
Specimen holderCryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image recordingAverage exposure time: 6.43 sec. / Electron dose: 40 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k) / Num. of grids imaged: 1 / Num. of real images: 601
Image scansWidth: 4096 / Height: 4096

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Processing

EM software
IDNameVersionCategory
1RELIONparticle selection
4CTFFIND4.1CTF correction
7PHENIXmodel fitting
9PHENIXdev_5294model refinement
10cryoSPARCinitial Euler assignment
11cryoSPARCfinal Euler assignment
12cryoSPARCclassification
13cryoSPARC3D reconstruction
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Particle selectionNum. of particles selected: 301423
SymmetryPoint symmetry: C5 (5 fold cyclic)
3D reconstructionResolution: 2.5 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 31000 / Symmetry type: POINT
Atomic model buildingB value: 60 / Protocol: OTHER / Space: REAL / Target criteria: FSC at 0.5
Atomic model buildingPDB-ID: 7A5V
Accession code: 7A5V / Source name: PDB / Type: experimental model
RefinementCross valid method: NONE
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
Displacement parametersBiso mean: 30.95 Å2
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.001414985
ELECTRON MICROSCOPYf_angle_d0.384720410
ELECTRON MICROSCOPYf_chiral_restr0.03942400
ELECTRON MICROSCOPYf_plane_restr0.00292505
ELECTRON MICROSCOPYf_dihedral_angle_d8.00035625
Refine LS restraints NCS
Ens-IDDom-IDAsym-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2EEELECTRON MICROSCOPYNCS constraints4.14974532076E-11
ens_1d_3EEELECTRON MICROSCOPYNCS constraints5.67857384437E-13
ens_1d_4EEELECTRON MICROSCOPYNCS constraints8.03368443011E-13
ens_1d_5EEELECTRON MICROSCOPYNCS constraints1.00804830201E-12
ens_2d_2NeELECTRON MICROSCOPYNCS constraints2.0444042376E-10
ens_2d_3NeELECTRON MICROSCOPYNCS constraints2.07178319889E-13
ens_2d_4NeELECTRON MICROSCOPYNCS constraints1.71980643815E-12
ens_2d_5NeELECTRON MICROSCOPYNCS constraints1.21005118623E-13

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