endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters / micrococcal nuclease / nucleic acid binding / extracellular region / metal ion binding / cytoplasm Similarity search - Function
Thermonuclease family signature 1. / Thermonuclease active site / Thermonuclease family signature 2. / Staphylococcal nuclease (SNase-like), OB-fold / Staphylococcal nuclease homologue / Thermonuclease domain profile. / Staphylococcal nuclease homologues / SNase-like, OB-fold superfamily Similarity search - Domain/homology
Monochromator: M / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 1.5418 Å / Relative weight: 1
Reflection
Resolution: 2→21.92 Å / Num. obs: 9707 / % possible obs: 99.9 % / Redundancy: 10.4 % / Biso Wilson estimate: 20.71 Å2 / Rmerge(I) obs: 0.013 / Rsym value: 0.086 / Net I/σ(I): 76.43
Reflection shell
Resolution: 2→2.07 Å / Redundancy: 10.1 % / Rmerge(I) obs: 0.094 / Mean I/σ(I) obs: 11.37 / Num. unique obs: 799 / Rsym value: 0.208 / % possible all: 100
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Processing
Software
Name
Version
Classification
REFMAC
5.8.0425
refinement
CrysalisPro
42.80a 64-bit (release 13-01-2023)
datascaling
CrysalisPro
42.80a 64-bit (release 13-01-2023)
datareduction
PHASER
2.8.3
phasing
Refinement
Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2→21.7 Å / Cor.coef. Fo:Fc: 0.957 / Cor.coef. Fo:Fc free: 0.929 / SU B: 4.585 / SU ML: 0.13 / Cross valid method: THROUGHOUT / ESU R: 0.184 / ESU R Free: 0.165 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.2324
461
4.8 %
RANDOM
Rwork
0.18491
-
-
-
obs
0.1872
9232
99.9 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK