+Open data
-Basic information
Entry | Database: PDB / ID: 9cef | ||||||||||||||||||
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Title | Caulobacter crescentus FljN flagellar filament (symmetrized) | ||||||||||||||||||
Components | Flagellin FljN | ||||||||||||||||||
Keywords | STRUCTURAL PROTEIN / flagellin / flagellar filament | ||||||||||||||||||
Function / homology | Flagellin, C-terminal domain / Bacterial flagellin C-terminal helical region / Flagellin / Flagellin, N-terminal domain / Bacterial flagellin N-terminal helical region / bacterial-type flagellum / structural molecule activity / extracellular region / Flagellin FljN Function and homology information | ||||||||||||||||||
Biological species | Caulobacter vibrioides (bacteria) | ||||||||||||||||||
Method | ELECTRON MICROSCOPY / helical reconstruction / cryo EM / Resolution: 2.22 Å | ||||||||||||||||||
Authors | Sanchez, J.C. / Montemayor, E.J. / Ploscariu, N.T. / Parrell, D. / Baumgardt, J.K. / Yang, J.E. / Sibert, B. / Cai, K. / Wright, E.R. | ||||||||||||||||||
Funding support | United States, 5items
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Citation | Journal: To Be Published Title: Direct evidence for multi-flagellin filament stabilization via atomic-level architecture of Caulobacter crescentus flagellar filaments Authors: Sanchez, J.C. / Montemayor, E.J. / Ploscariu, N.T. / Parrell, D. / Baumgardt, J.K. / Yang, J.E. / Sibert, B. / Cai, K. / Wright, E.R. | ||||||||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 9cef.cif.gz | 1.9 MB | Display | PDBx/mmCIF format |
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PDB format | pdb9cef.ent.gz | 1.6 MB | Display | PDB format |
PDBx/mmJSON format | 9cef.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 9cef_validation.pdf.gz | 1.3 MB | Display | wwPDB validaton report |
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Full document | 9cef_full_validation.pdf.gz | 1.3 MB | Display | |
Data in XML | 9cef_validation.xml.gz | 230.7 KB | Display | |
Data in CIF | 9cef_validation.cif.gz | 351.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ce/9cef ftp://data.pdbj.org/pub/pdb/validation_reports/ce/9cef | HTTPS FTP |
-Related structure data
Related structure data | 45500MC 8uxkC 8uxnC 9cejC 9cemC 9ceoC 9cepC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 27949.256 Da / Num. of mol.: 44 / Source method: isolated from a natural source / Source: (natural) Caulobacter vibrioides (bacteria) / Strain: NA1000 / References: UniProt: O52530 |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: FILAMENT / 3D reconstruction method: helical reconstruction |
-Sample preparation
Component | Name: FljN flagellar filament (symmetrized) / Type: COMPLEX / Entity ID: all / Source: NATURAL |
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Molecular weight | Value: 0.28 kDa/nm / Experimental value: NO |
Source (natural) | Organism: Caulobacter vibrioides (bacteria) / Strain: NA1000 |
Buffer solution | pH: 7.4 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 95 % / Chamber temperature: 293 K |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: TFS KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal magnification: 105000 X / Nominal defocus max: 2500 nm / Nominal defocus min: 500 nm / C2 aperture diameter: 70 µm |
Specimen holder | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER |
Image recording | Electron dose: 55 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
EM imaging optics | Energyfilter name: GIF Bioquantum / Energyfilter slit width: 20 eV |
-Processing
EM software |
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
Helical symmerty | Angular rotation/subunit: 65.923 ° / Axial rise/subunit: 4.778 Å / Axial symmetry: C1 | ||||||||||||||||||||||||
3D reconstruction | Resolution: 2.22 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 131070 / Symmetry type: HELICAL | ||||||||||||||||||||||||
Atomic model building | B value: 67 / Space: REAL | ||||||||||||||||||||||||
Atomic model building | Accession code: AF-O52530-F1 / Source name: AlphaFold / Type: in silico model | ||||||||||||||||||||||||
Refine LS restraints |
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