positive regulation of compound eye pigmentation / positive regulation of locomotor rhythm / PINK1-PRKN Mediated Mitophagy / Josephin domain DUBs / sperm mitochondrion organization / larval locomotory behavior / Antigen processing: Ubiquitination & Proteasome degradation / Aggrephagy / parkin-mediated stimulation of mitophagy in response to mitochondrial depolarization / positive regulation of neuronal action potential ...positive regulation of compound eye pigmentation / positive regulation of locomotor rhythm / PINK1-PRKN Mediated Mitophagy / Josephin domain DUBs / sperm mitochondrion organization / larval locomotory behavior / Antigen processing: Ubiquitination & Proteasome degradation / Aggrephagy / parkin-mediated stimulation of mitophagy in response to mitochondrial depolarization / positive regulation of neuronal action potential / negative regulation of mitochondrial fusion / RBR-type E3 ubiquitin transferase / positive regulation of autophagy of mitochondrion / positive regulation of mitophagy / regulation of cellular response to oxidative stress / autophagy of mitochondrion / negative regulation of JNK cascade / mitochondrial fission / ubiquitin conjugating enzyme binding / regulation of mitochondrion organization / hypothalamus gonadotrophin-releasing hormone neuron development / female meiosis I / positive regulation of protein monoubiquitination / mitochondrion transport along microtubule / fat pad development / oogenesis / positive regulation of mitochondrial fission / female gonad development / seminiferous tubule development / male meiosis I / protein monoubiquitination / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator / protein autoubiquitination / regulation of neuron apoptotic process / energy homeostasis / regulation of proteasomal protein catabolic process / ubiquitin ligase complex / Maturation of protein E / Maturation of protein E / ER Quality Control Compartment (ERQC) / Myoclonic epilepsy of Lafora / FLT3 signaling by CBL mutants / Prevention of phagosomal-lysosomal fusion / IRAK2 mediated activation of TAK1 complex / Regulation of TBK1, IKKε (IKBKE)-mediated activation of IRF3, IRF7 / Alpha-protein kinase 1 signaling pathway / Glycogen synthesis / IRAK1 recruits IKK complex / IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation / Regulation of TBK1, IKKε-mediated activation of IRF3, IRF7 upon TLR3 ligation / Membrane binding and targetting of GAG proteins / Endosomal Sorting Complex Required For Transport (ESCRT) / IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation / PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1 / Negative regulation of FLT3 / Constitutive Signaling by NOTCH1 HD Domain Mutants / Regulation of FZD by ubiquitination / TICAM1,TRAF6-dependent induction of TAK1 complex / TICAM1-dependent activation of IRF3/IRF7 / NOTCH2 Activation and Transmission of Signal to the Nucleus / APC/C:Cdc20 mediated degradation of Cyclin B / p75NTR recruits signalling complexes / Downregulation of ERBB4 signaling / APC-Cdc20 mediated degradation of Nek2A / TRAF6-mediated induction of TAK1 complex within TLR4 complex / TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling / PINK1-PRKN Mediated Mitophagy / Pexophagy / Regulation of innate immune responses to cytosolic DNA / Regulation of pyruvate metabolism / InlA-mediated entry of Listeria monocytogenes into host cells / VLDLR internalisation and degradation / Downregulation of ERBB2:ERBB3 signaling / NF-kB is activated and signals survival / Regulation of PTEN localization / NRIF signals cell death from the nucleus / Regulation of BACH1 activity / Activated NOTCH1 Transmits Signal to the Nucleus / Synthesis of active ubiquitin: roles of E1 and E2 enzymes / Translesion synthesis by REV1 / MAP3K8 (TPL2)-dependent MAPK1/3 activation / Translesion synthesis by POLK / TICAM1, RIP1-mediated IKK complex recruitment / adult locomotory behavior / Downregulation of TGF-beta receptor signaling / neuron projection morphogenesis / Activation of IRF3, IRF7 mediated by TBK1, IKKε (IKBKE) / Translesion synthesis by POLI / Josephin domain DUBs / Gap-filling DNA repair synthesis and ligation in GG-NER / Regulation of activated PAK-2p34 by proteasome mediated degradation / InlB-mediated entry of Listeria monocytogenes into host cell / IKK complex recruitment mediated by RIP1 / JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 / negative regulation of protein phosphorylation / regulation of mitochondrial membrane potential / TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) / mitochondrion organization / N-glycan trimming in the ER and Calnexin/Calreticulin cycle / TNFR1-induced NF-kappa-B signaling pathway Similarity search - Function
: / : / : / E3 ubiquitin-protein ligase parkin / RING/Ubox-like zinc-binding domain / Parkin, RING/Ubox like zinc-binding domain / RING/Ubox like zinc-binding domain / RING/Ubox like zinc-binding domain / E3 ubiquitin ligase RBR family / IBR domain ...: / : / : / E3 ubiquitin-protein ligase parkin / RING/Ubox-like zinc-binding domain / Parkin, RING/Ubox like zinc-binding domain / RING/Ubox like zinc-binding domain / RING/Ubox like zinc-binding domain / E3 ubiquitin ligase RBR family / IBR domain / In Between Ring fingers / TRIAD supradomain / TRIAD supradomain profile. / : / Ubiquitin domain signature. / Ubiquitin conserved site / Ubiquitin domain / Ubiquitin family / Ubiquitin homologues / Ubiquitin domain profile. / Ubiquitin-like domain / Zinc finger, RING/FYVE/PHD-type / Ubiquitin-like domain superfamily Similarity search - Domain/homology
Mass: 138.006 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Formula: C3H8BrN / Feature type: SUBJECT OF INVESTIGATION
Has ligand of interest
Y
-
Experimental details
-
Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Sample state
Spectrometer-ID
Type
1
1
2
isotropic
1
3D HN(CA)CB
1
2
2
isotropic
1
3DCBCA(CO)NH
1
7
1
isotropic
1
3D HN(CA)CB
1
8
1
isotropic
1
3D HNCA
1
5
1
isotropic
1
2D 1H-15N HSQC
1
12
1
isotropic
1
2D 1H-13C HSQC
1
3
3
isotropic
1
3D HN(CA)CB
1
4
3
isotropic
1
3DCBCA(CO)NH
1
11
3
isotropic
1
3D HNCA
1
6
3
isotropic
1
2D 1H-15N HSQC
1
13
3
isotropic
1
2D 1H-13C HSQC
1
9
4
isotropic
1
2D 1H-15N HSQC
1
10
5
isotropic
1
2D 1H-15N HSQC
-
Sample preparation
Details
Type
Solution-ID
Contents
Details
Label
Solvent system
solution
1
300 uM [U-13C; U-15N] Rcat domain from Parkin, 300 uM Ubiquitin G75-C3, 90% H2O/10% D2O
Rcat-Ub Covalent complex with 13C,15N-labelled Rcat domain from fly parkin (aa 410-482) linked to unlabelled Ub G75-C3.
13C,15N-Rcat-Ub
90% H2O/10% D2O
solution
2
800 uM [U-13C; U-15N] Rcat domain from Parkin, 800 uM Ubiquitin G75-C3, 90% H2O/10% D2O
Rcat-Ub Covalent complex with 13C,15N-labelled Rcat domain from fly parkin (aa 410-482) linked to unlabelled Ub G75-C3.
13C,15N-Rcat-Ub2
90% H2O/10% D2O
solution
3
300 uM [U-13C; U-15N] Ubiquitin G75-C3, 300 uM Rcat domain from Parkin, 90% H2O/10% D2O
Rcat-Ub Covalent complex with unlabelled Rcat domain from fly parkin (aa 410-482) linked to 13C,15N-labelled Ub G75-C3.
Rcat-15N,13C-Ub
90% H2O/10% D2O
solution
4
300 uM [U-13C; U-15N] Rcat domain from Parkin, 90% H2O/10% D2O
13C,15N-Rcat domain from fly parkin (aa 410-482) in absence of Ub.
13C,15N-Rcat
90% H2O/10% D2O
solution
5
500 uM [U-13C; U-15N] Ubiquitin G75-C3, 90% H2O/10% D2O
13C,15N-Ub G75-C3 in absence of Rcat.
13C,15N-Ub-C3
90% H2O/10% D2O
Sample
Conc. (mg/ml)
Component
Isotopic labeling
Solution-ID
300uM
RcatdomainfromParkin
[U-13C; U-15N]
1
300uM
Ubiquitin G75-C3
naturalabundance
1
800uM
RcatdomainfromParkin
[U-13C; U-15N]
2
800uM
Ubiquitin G75-C3
naturalabundance
2
300uM
Ubiquitin G75-C3
[U-13C; U-15N]
3
300uM
RcatdomainfromParkin
naturalabundance
3
300uM
RcatdomainfromParkin
[U-13C; U-15N]
4
500uM
Ubiquitin G75-C3
[U-13C; U-15N]
5
Sample conditions
Details: 25 mM HEPES, 0.5 mM TCEP / Ionic strength: 100 mM / Label: Conditions_1 / pH: 7 / Pressure: 1 atm / Temperature: 298 K
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