ソフトウェア | 名称 | バージョン | 分類 |
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PHENIX | 1.20.1_4487精密化 | HKL-2000 | | データスケーリング | HKL-2000 | | データ削減 | PHASER | | 位相決定 | |
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精密化 | 構造決定の手法: 分子置換 / 解像度: 2.8→48.91 Å / SU ML: 0.49 / 交差検証法: FREE R-VALUE / σ(F): 1.37 / 位相誤差: 30.45 / 立体化学のターゲット値: ML
| Rfactor | 反射数 | %反射 |
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Rfree | 0.2881 | 1688 | 4.82 % |
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Rwork | 0.234 | - | - |
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obs | 0.2366 | 35013 | 98.29 % |
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溶媒の処理 | 減衰半径: 0.9 Å / VDWプローブ半径: 1.1 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL |
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精密化ステップ | サイクル: LAST / 解像度: 2.8→48.91 Å
| タンパク質 | 核酸 | リガンド | 溶媒 | 全体 |
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原子数 | 8654 | 0 | 0 | 72 | 8726 |
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拘束条件 | Refine-ID | タイプ | Dev ideal | 数 |
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X-RAY DIFFRACTION | f_bond_d0.003 | 8813 | X-RAY DIFFRACTION | f_angle_d0.718 | 11938 | X-RAY DIFFRACTION | f_dihedral_angle_d6.934 | 1187 | X-RAY DIFFRACTION | f_chiral_restr0.042 | 1393 | X-RAY DIFFRACTION | f_plane_restr0.006 | 1495 | | | | | |
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LS精密化 シェル | 解像度 (Å) | Rfactor Rfree | Num. reflection Rfree | Rfactor Rwork | Num. reflection Rwork | Refine-ID | % reflection obs (%) |
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2.8-2.89 | 0.4133 | 123 | 0.378 | 2360 | X-RAY DIFFRACTION | 85 | 2.89-2.98 | 0.3899 | 142 | 0.3297 | 2654 | X-RAY DIFFRACTION | 96 | 2.98-3.09 | 0.3718 | 144 | 0.3176 | 2781 | X-RAY DIFFRACTION | 100 | 3.09-3.21 | 0.3617 | 138 | 0.2775 | 2791 | X-RAY DIFFRACTION | 100 | 3.21-3.36 | 0.3595 | 139 | 0.2887 | 2794 | X-RAY DIFFRACTION | 100 | 3.36-3.53 | 0.3277 | 140 | 0.2855 | 2785 | X-RAY DIFFRACTION | 100 | 3.53-3.75 | 0.3011 | 141 | 0.2582 | 2816 | X-RAY DIFFRACTION | 100 | 3.75-4.04 | 0.2997 | 141 | 0.2202 | 2796 | X-RAY DIFFRACTION | 100 | 4.04-4.45 | 0.2561 | 161 | 0.1973 | 2811 | X-RAY DIFFRACTION | 100 | 4.45-5.09 | 0.2357 | 146 | 0.1778 | 2855 | X-RAY DIFFRACTION | 100 | 5.09-6.41 | 0.2762 | 136 | 0.2185 | 2887 | X-RAY DIFFRACTION | 100 | 6.42-48.91 | 0.2417 | 137 | 0.211 | 2995 | X-RAY DIFFRACTION | 99 |
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精密化 TLS | 手法: refined / Refine-ID: X-RAY DIFFRACTION ID | L11 (°2) | L12 (°2) | L13 (°2) | L22 (°2) | L23 (°2) | L33 (°2) | S11 (Å °) | S12 (Å °) | S13 (Å °) | S21 (Å °) | S22 (Å °) | S23 (Å °) | S31 (Å °) | S32 (Å °) | S33 (Å °) | T11 (Å2) | T12 (Å2) | T13 (Å2) | T22 (Å2) | T23 (Å2) | T33 (Å2) | Origin x (Å) | Origin y (Å) | Origin z (Å) |
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1 | 4.2826 | -1.2914 | -0.3566 | 4.5882 | 1.341 | 2.2955 | 0.1375 | 0.3476 | 0.181 | -0.0488 | -0.1085 | 0.5884 | -0.1461 | -0.3421 | -0.0116 | 0.2615 | -0.0332 | 0.0563 | 0.4028 | 0.0418 | 0.4046 | -20.8865 | 41.2206 | -33.8012 | 2 | 5.6765 | -1.3702 | -0.8435 | 7.0819 | 0.4299 | 3.6061 | -0.475 | -1.0177 | -0.6503 | 2.1306 | -0.0267 | 0.1361 | -0.3094 | -0.6812 | 0.4768 | 0.9225 | 0.0532 | 0.4483 | 0.8753 | -0.087 | 1.1494 | -37.129 | 47.0272 | -6.6873 | 3 | 9.6337 | -1.595 | 2.7955 | 7.2635 | -1.5212 | 8.8204 | 0.0178 | -0.6908 | 0.2045 | 2.5208 | -0.2545 | 0.801 | -0.4268 | -0.4864 | 0.203 | 1.1608 | 0.0437 | 0.3553 | 0.5668 | -0.0173 | 0.837 | -32.6832 | 46.1889 | -11.3174 | 4 | 4.6651 | -0.3022 | 0.188 | 2.3744 | 0.2358 | 3.1986 | -0.0408 | -0.2428 | 0.2103 | 1.7982 | -0.0605 | 0.8944 | 0.114 | -0.0182 | 0.1619 | 1.2201 | 0.0072 | 0.5469 | 0.6666 | -0.0676 | 1.043 | -33.3972 | 40.9788 | -8.1908 | 5 | 4.6443 | 0.5356 | -4.5059 | 0.1794 | -0.6231 | 4.8122 | -0.3878 | -1.7549 | 0.7147 | 2.5983 | -0.6374 | -0.4053 | -0.3987 | 1.1265 | 0.8373 | 2.7478 | 0.0146 | 0.0991 | 1.041 | -0.1541 | 0.8745 | -29.1546 | 40.364 | 6.0879 | 6 | 6.639 | -0.2683 | 0.7923 | 4.3141 | 0.5109 | 4.1218 | -0.0095 | -0.6145 | 1.3038 | 2.0807 | -0.5555 | 1.0414 | -0.0096 | -0.3457 | 0.3592 | 1.3809 | 0.0306 | 0.5386 | 0.7343 | -0.1289 | 1.2126 | -28.7932 | 51.8256 | -9.0414 | 7 | 5.0511 | -0.5092 | -0.0105 | 4.5777 | 0.3352 | 3.2518 | -0.2885 | 0.8055 | -0.838 | 0.5897 | 0.0783 | 0.8541 | 0.4896 | -1.0796 | 0.1022 | 0.6342 | -0.3008 | 0.3461 | 0.6295 | -0.2361 | 1.0868 | -31.2292 | 19.3323 | -28.9415 | 8 | 4.9743 | -1.9983 | -0.5879 | 4.7285 | -3.8517 | 4.2835 | -0.9841 | 0.4524 | -1.175 | 1.4561 | -0.1691 | 0.6508 | 1.7397 | -0.2334 | 0.5718 | 1.2636 | -0.2498 | 0.43 | 0.4822 | -0.0254 | 0.8699 | -25.8232 | 13.156 | -24.8458 | 9 | 3.5072 | -1.5597 | -2.0625 | 7.1179 | 1.3516 | 4.9406 | -0.1731 | -0.0156 | -0.5424 | 0.8166 | 0.1021 | 0.4185 | 0.9407 | -0.3052 | 0.0529 | 0.4198 | -0.1373 | 0.0775 | 0.3629 | -0.0583 | 0.4989 | -20.5229 | 22.0843 | -28.5646 | 10 | 1.4542 | 0.6979 | 0.2768 | 0.7332 | -0.1302 | 0.3661 | -1.5972 | -0.6398 | 0.5215 | 1.9196 | 0.3123 | 1.4942 | 0.2354 | -0.1992 | 0.0943 | 1.5592 | -0.1053 | 1.2961 | 0.751 | 0.0573 | 0.9459 | -38.3818 | 34.1258 | -2.1185 | 11 | 4.0672 | -1.25 | 1.8369 | 3.8581 | 3.3606 | 5.5494 | 0.1279 | -1.2593 | -0.9235 | -0.7058 | -0.928 | -0.7064 | 1.1933 | -0.8131 | 0.7621 | 1.7491 | -0.2151 | 0.8623 | 1.1235 | 0.0694 | 1.3774 | -43.3513 | 30.0872 | -2.8069 | 12 | 4.7413 | -0.9391 | 1.2957 | 1.4657 | 0.4213 | 0.8315 | -0.2692 | -1.2731 | 0.5262 | -0.5957 | 0.0178 | 0.5091 | 0.3047 | -0.127 | 0.134 | 1.676 | -0.3165 | 0.886 | 0.9776 | -0.1307 | 1.255 | -43.6797 | 35.4429 | -4.4491 | 13 | 3.0357 | -1.8536 | 0.7557 | 5.1164 | 1.0085 | 1.5666 | -0.2172 | -0.4575 | -0.8522 | -0.6184 | 0.3344 | -0.2729 | 0.6892 | -1.4019 | -0.0633 | 1.3807 | -0.0925 | 0.6647 | 1.5115 | 0.1448 | 1.3433 | -48.8008 | 29.5952 | 4.2491 | 14 | 2.6741 | 1.0508 | 2.9848 | 3.2437 | 0.7723 | 6.9747 | 0.0688 | -0.2237 | -0.1491 | -0.225 | 0.0187 | 0.0184 | -0.22 | -0.6639 | -0.0798 | 0.2526 | 0.0342 | 0.1046 | 0.3421 | 0.0165 | 0.3954 | -5.8567 | 4.5728 | -71.6841 | 15 | 1.8993 | 0.8627 | 0.356 | 0.8139 | -0.0728 | 3.2504 | -0.0088 | 0.4354 | 0.047 | -0.5284 | 0.3365 | -0.1099 | -0.1551 | 0.095 | -0.3435 | 0.571 | 0.0649 | 0.1243 | 0.4699 | 0.0034 | 0.4328 | -3.5271 | 7.7773 | -87.5333 | 16 | 1.6879 | 1.316 | 1.142 | 6.3516 | -2.6535 | 4.4867 | 0.0323 | 1.6526 | -0.8297 | -0.2299 | 0.0598 | -1.0784 | -0.665 | 0.603 | -0.1385 | 0.9311 | -0.1206 | 0.4491 | 1.0368 | -0.0391 | 0.9896 | 0.7023 | 6.3909 | -105.0918 | 17 | 7.5073 | -3.4839 | 0.0681 | 2.9166 | 1.2492 | 1.3246 | 0.0436 | 0.4593 | -1.3288 | -1.0855 | -0.6546 | -0.2374 | 0.6595 | 1.7237 | 0.6534 | 0.9558 | -0.209 | 0.3459 | 1.3438 | -0.172 | 1.1166 | 5.4612 | 0.8246 | -111.2593 | 18 | 2.6758 | 0.2294 | 0.5787 | 2.7396 | -0.5669 | 7.6756 | -0.0614 | 0.3983 | 1.1033 | -1.0861 | 0.1776 | -0.4415 | -1.743 | -0.1935 | -0.1348 | 1.0345 | 0.0582 | 0.2277 | 0.4708 | 0.0608 | 0.6567 | -0.3402 | 28.224 | -77.9945 | 19 | 1.3666 | 0.3102 | 0.081 | 0.3026 | 0.505 | 3.602 | -0.405 | 0.6817 | 0.3764 | -1.4428 | 0.4555 | 0.095 | -0.0792 | -0.2401 | -0.0658 | 1.8784 | -0.0375 | 0.0823 | 0.6755 | 0.1172 | 0.5636 | -2.2999 | 19.8836 | -94.0984 | 20 | 1.5454 | -1.6442 | 1.2947 | 1.6335 | -0.9005 | 4.1558 | -0.128 | 0.3585 | 0.1164 | -1.6673 | 0.2569 | -0.0059 | -0.5917 | -0.1946 | -0.2124 | 2.2988 | -0.2288 | 0.0723 | 1.105 | 0.1278 | 0.6483 | -9.1933 | 17.755 | -112.101 | 21 | 5.7605 | -1.7173 | -5.0735 | 2.0088 | 1.2081 | 4.526 | 0.4126 | 0.2479 | 0.5726 | 0.2288 | 0.5362 | 1.4769 | 0.6999 | -0.7086 | -0.8099 | 0.4073 | 0.0679 | 0.1841 | 0.7026 | 0.0286 | 0.7694 | -16.0514 | 18.7304 | -49.9559 | 22 | 2.3599 | 0.5403 | 1.721 | 2.4761 | 0.9013 | 2.2264 | 0.4957 | -0.0361 | 2.0121 | 0.312 | -0.3584 | -0.722 | 0.0375 | 0.4974 | -0.0964 | 0.4182 | 0.0461 | 0.0127 | 0.5915 | -0.1255 | 1.1623 | 20.1867 | 24.647 | -43.3291 | 23 | 7.0537 | 1.2964 | 4.9574 | 6.1336 | 0.5273 | 6.4212 | 0.448 | -2.0985 | -0.1204 | 0.5471 | -0.3693 | -0.4608 | 0.6721 | -0.331 | -0.013 | 0.5097 | -0.0155 | -0.0735 | 0.6149 | -0.0572 | 0.3803 | 17.4137 | 15.8516 | -35.6853 | 24 | 4.2118 | -1.231 | 2.8355 | 9.4514 | 0.7473 | 2.1862 | 0.9952 | -0.8111 | -1.681 | 0.0669 | -0.0911 | 1.9035 | 1.306 | -2.3752 | -0.7452 | 0.7866 | -0.3167 | -0.3289 | 1.2136 | -0.0079 | 0.8643 | -1.4187 | 9.1131 | -45.9551 | 25 | 7.3244 | -1.9586 | 3.953 | 4.0718 | -0.4576 | 6.2984 | 0.8985 | 0.0785 | -1.0748 | 0.5927 | -0.1147 | -0.8981 | 1.313 | 0.8313 | -0.7917 | 0.6693 | 0.1677 | -0.1878 | 0.6163 | -0.1379 | 0.8888 | 23.2482 | 9.3104 | -39.6149 | 26 | 1.858 | -0.9736 | 1.7591 | 3.2037 | 3.4904 | 8.8762 | 0.7644 | 0.9145 | -1.0795 | -0.1213 | 0.1677 | -1.0228 | 1.5079 | 0.1303 | -0.8237 | 0.6316 | 0.0074 | 0.0744 | 0.7009 | -0.0677 | 1.1184 | 29.8177 | 13.1228 | -47.3829 | 27 | 3.7998 | -0.7926 | 4.8413 | 2.4938 | -0.6942 | 5.8219 | 1.205 | 0.7217 | -0.6357 | 0.0971 | -0.5391 | -0.3182 | 0.566 | -0.0307 | -0.529 | 0.3437 | 0.0785 | 0.0473 | 0.5427 | -0.0571 | 0.3939 | 5.421 | 14.1439 | -52.6241 | 28 | 2.8997 | -2.2013 | 3.6279 | 3.3571 | -2.695 | 3.7376 | -0.1681 | -0.0384 | 0.9334 | -0.4058 | -0.3745 | -0.8695 | 0.4455 | 0.2852 | 0.3767 | 0.3321 | 0.001 | 0.0867 | 0.5789 | -0.127 | 0.6044 | 3.8592 | 28.0509 | -46.111 | 29 | 2.7971 | -2.3407 | 3.327 | 3.9206 | -1.5194 | 4.7673 | 0.3642 | -1.1132 | 0.4514 | 0.1152 | 0.2122 | -0.7128 | 0.4281 | -0.049 | -0.2981 | 0.421 | -0.0435 | 0.0211 | 0.9082 | -0.1579 | 1.0416 | 22.4077 | 26.2011 | -36.8831 | 30 | 3.7004 | -0.1961 | 3.3909 | 1.0335 | -0.7264 | 3.3414 | -0.3654 | -0.351 | 2.0607 | -0.0059 | 0.3097 | -0.8031 | -0.5162 | 0.2208 | 0.1731 | 0.4013 | -0.096 | 0.0485 | 0.7355 | -0.2978 | 2.1355 | 21.6041 | 39.2109 | -37.5519 | 31 | 0.278 | -1.1881 | 0.3891 | 8.6048 | -1.3491 | 3.3478 | -0.2801 | -1.5559 | 1.4883 | 0.8052 | 0.696 | -1.4417 | -0.3506 | 1.2831 | -0.2933 | 0.5126 | -0.0092 | -0.0883 | 1.1622 | -0.4897 | 1.0551 | 22.9773 | 33.7246 | -25.5843 | 32 | 8.6625 | 3.779 | 5.9093 | 4.1107 | 2.6519 | 8.3209 | 0.4585 | -0.9349 | 1.6553 | 0.228 | -0.3885 | -0.4095 | 0.1016 | -0.0163 | 0.1922 | 0.3427 | -0.1223 | 0.1452 | 0.6779 | -0.3058 | 0.7772 | -0.7753 | 33.9434 | -40.6766 |
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精密化 TLSグループ | ID | Refine-ID | Refine TLS-ID | Selection details |
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1 | X-RAY DIFFRACTION | 1 | chain 'H' and (resid 1 through 119 )2 | X-RAY DIFFRACTION | 2 | chain 'H' and (resid 120 through 153 )3 | X-RAY DIFFRACTION | 3 | chain 'H' and (resid 154 through 164 )4 | X-RAY DIFFRACTION | 4 | chain 'H' and (resid 165 through 187 )5 | X-RAY DIFFRACTION | 5 | chain 'H' and (resid 188 through 205 )6 | X-RAY DIFFRACTION | 6 | chain 'H' and (resid 206 through 217 )7 | X-RAY DIFFRACTION | 7 | chain 'L' and (resid -1 through 12 ) | 8 | X-RAY DIFFRACTION | 8 | chain 'L' and (resid 13 through 23 )9 | X-RAY DIFFRACTION | 9 | chain 'L' and (resid 24 through 99 )10 | X-RAY DIFFRACTION | 10 | chain 'L' and (resid 100 through 126 )11 | X-RAY DIFFRACTION | 11 | chain 'L' and (resid 127 through 147 )12 | X-RAY DIFFRACTION | 12 | chain 'L' and (resid 148 through 186 )13 | X-RAY DIFFRACTION | 13 | chain 'L' and (resid 187 through 208 )14 | X-RAY DIFFRACTION | 14 | chain 'A' and (resid 1 through 83 )15 | X-RAY DIFFRACTION | 15 | chain 'A' and (resid 84 through 155 )16 | X-RAY DIFFRACTION | 16 | chain 'A' and (resid 156 through 194 )17 | X-RAY DIFFRACTION | 17 | chain 'A' | | | | | | | | | | | | | | | |
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