ソフトウェア | 名称 | バージョン | 分類 |
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PHENIX | 1.20.1_4487精密化 | HKL-2000 | | データ削減 | HKL-2000 | | データスケーリング | PHASER | | 位相決定 | |
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精密化 | 構造決定の手法: 分子置換 / 解像度: 2.6→47.57 Å / SU ML: 0.3452 / 交差検証法: FREE R-VALUE / σ(F): 1.36 / 位相誤差: 25.2688 立体化学のターゲット値: GeoStd + Monomer Library + CDL v1.2
| Rfactor | 反射数 | %反射 |
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Rfree | 0.2508 | 2083 | 4.95 % |
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Rwork | 0.1958 | 39993 | - |
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obs | 0.1985 | 42076 | 96.22 % |
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溶媒の処理 | 減衰半径: 0.9 Å / VDWプローブ半径: 1.1 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL |
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原子変位パラメータ | Biso mean: 54.04 Å2 |
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精密化ステップ | サイクル: LAST / 解像度: 2.6→47.57 Å
| タンパク質 | 核酸 | リガンド | 溶媒 | 全体 |
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原子数 | 8960 | 0 | 0 | 233 | 9193 |
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拘束条件 | Refine-ID | タイプ | Dev ideal | 数 |
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X-RAY DIFFRACTION | f_bond_d0.002 | 9133 | X-RAY DIFFRACTION | f_angle_d0.5609 | 12382 | X-RAY DIFFRACTION | f_chiral_restr0.0399 | 1435 | X-RAY DIFFRACTION | f_plane_restr0.0039 | 1563 | X-RAY DIFFRACTION | f_dihedral_angle_d5.1807 | 1236 | | | | | |
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LS精密化 シェル | 解像度 (Å) | Rfactor Rfree | Num. reflection Rfree | Rfactor Rwork | Num. reflection Rwork | Refine-ID | % reflection obs (%) |
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2.6-2.66 | 0.3305 | 93 | 0.2516 | 1688 | X-RAY DIFFRACTION | 61.5 | 2.66-2.73 | 0.3115 | 130 | 0.2621 | 2479 | X-RAY DIFFRACTION | 92 | 2.73-2.8 | 0.3321 | 131 | 0.2669 | 2614 | X-RAY DIFFRACTION | 95.02 | 2.8-2.88 | 0.3248 | 126 | 0.2585 | 2632 | X-RAY DIFFRACTION | 96.53 | 2.88-2.97 | 0.3627 | 136 | 0.2588 | 2721 | X-RAY DIFFRACTION | 98.82 | 2.97-3.08 | 0.3428 | 125 | 0.242 | 2749 | X-RAY DIFFRACTION | 99.93 | 3.08-3.2 | 0.3084 | 132 | 0.2283 | 2768 | X-RAY DIFFRACTION | 100 | 3.2-3.35 | 0.2737 | 155 | 0.2159 | 2727 | X-RAY DIFFRACTION | 99.97 | 3.35-3.53 | 0.265 | 174 | 0.2136 | 2721 | X-RAY DIFFRACTION | 100 | 3.53-3.75 | 0.2261 | 160 | 0.1978 | 2742 | X-RAY DIFFRACTION | 100 | 3.75-4.04 | 0.2463 | 163 | 0.1793 | 2750 | X-RAY DIFFRACTION | 100 | 4.04-4.44 | 0.235 | 114 | 0.1541 | 2825 | X-RAY DIFFRACTION | 100 | 4.44-5.08 | 0.1774 | 146 | 0.1438 | 2784 | X-RAY DIFFRACTION | 100 | 5.08-6.4 | 0.2114 | 147 | 0.1728 | 2844 | X-RAY DIFFRACTION | 100 | 6.4-47.57 | 0.1974 | 151 | 0.1726 | 2949 | X-RAY DIFFRACTION | 99.01 |
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精密化 TLS | 手法: refined / Refine-ID: X-RAY DIFFRACTION ID | L11 (°2) | L12 (°2) | L13 (°2) | L22 (°2) | L23 (°2) | L33 (°2) | S11 (Å °) | S12 (Å °) | S13 (Å °) | S21 (Å °) | S22 (Å °) | S23 (Å °) | S31 (Å °) | S32 (Å °) | S33 (Å °) | T11 (Å2) | T12 (Å2) | T13 (Å2) | T22 (Å2) | T23 (Å2) | T33 (Å2) | Origin x (Å) | Origin y (Å) | Origin z (Å) |
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1 | 2.19890707179 | 0.29050053278 | 0.421458475989 | 3.20600175207 | -0.116599437795 | 2.4570457573 | -0.0701084740276 | -0.102774851047 | -0.218696932661 | 0.000644702870596 | 0.235844141428 | 0.533944373284 | 0.148266128289 | -0.263473939688 | -0.105014962778 | 0.283862828076 | -0.027804059355 | 0.00466457433165 | 0.233741156006 | 0.0134555310005 | 0.321665701787 | -11.6625195209 | 6.43445719642 | 22.9382377989 | 2 | 3.58820484924 | 1.86794771553 | -1.13953897119 | 3.79637176884 | -0.165900862399 | 5.19710105357 | 0.164197038702 | 0.27523906403 | -0.366170821353 | -0.30781614307 | 0.106424220058 | -0.234096228175 | 0.579060940186 | 0.569926053253 | -0.130277219809 | 0.413416202134 | 0.135781508197 | -0.145724695773 | 0.371944105753 | -0.135886328387 | 0.429845675079 | -9.72532282147 | 3.2228355602 | -10.8487098537 | 3 | 2.44320900943 | -0.481023308393 | -0.580044741072 | 3.74950231138 | 0.222511263062 | 5.03921489129 | -0.249672570998 | 0.0707898625387 | 0.160660470548 | -0.00603942597215 | 0.214280394305 | 0.394626007574 | -0.092552144443 | -0.195355216245 | 0.00669486968847 | 0.298630754584 | -0.0151528818847 | -0.0800602148406 | 0.199893754409 | 0.0174813777006 | 0.343789038118 | -8.92219863195 | 27.1532975232 | 16.6916439424 | 4 | 5.30244567435 | 1.19849082821 | -4.57167897625 | 0.84996515718 | -0.425930982069 | 6.27588226457 | 0.326148715269 | 0.481990004941 | 0.470031028388 | 0.0412375531265 | -0.0961972001712 | 0.589842439608 | -1.46194125254 | -0.416827967258 | -0.251357427747 | 0.540413530472 | 0.0821270271948 | -0.0619505750796 | 0.217093317367 | -0.0546162665869 | 0.465323470485 | -9.40395784935 | 33.3800731317 | 20.0855324436 | 5 | 5.52373188124 | 3.88217795329 | -3.30785538498 | 3.95781319402 | -3.21949882857 | 7.78101229643 | -0.151267216713 | -0.0189845640385 | 0.170799945098 | -0.344037939323 | 0.173303772458 | 0.207456736301 | 0.322872693579 | -0.124293519032 | 0.00870014272416 | 0.349588470487 | 0.0692540428544 | -0.0811134223816 | 0.19965341024 | -0.0457774363636 | 0.337496332343 | -10.5079918376 | 23.6527567101 | 16.2911578786 | 6 | 1.61946394747 | -0.329482460683 | 0.635246653845 | 2.72393701446 | 3.55415887057 | 5.35545164694 | -0.0933910652434 | 0.489175414958 | 0.247169141345 | -0.615445295804 | 0.827531720345 | -0.284498278183 | 0.00404444796128 | -0.195061841482 | -0.607385632132 | 0.464672636959 | -0.175634337794 | -0.0326173532271 | 0.438786510204 | 0.0422479654268 | 0.240600194512 | -8.40494015307 | 29.1791014488 | -5.32261696244 | 7 | 4.67376984506 | 1.58792956114 | 1.52042049393 | 4.6100798222 | 0.0783168004997 | 3.59068316372 | 0.250589214251 | 0.617286332123 | -0.834518806344 | -0.2409367926 | 0.351605139047 | -0.492114359669 | 0.456501964784 | 0.361717286419 | -0.563900753517 | 0.493260763951 | 0.0381113373146 | -0.0328735779819 | 0.507248083956 | -0.155046772968 | 0.444012954575 | -17.5981886155 | 4.98988448028 | -21.1480367969 | 8 | 3.73535839285 | 0.793600512857 | 1.88209650554 | 2.23694017638 | 1.22312727939 | 2.38853443003 | 0.0591042318024 | 0.295821020114 | -0.118370433729 | 0.0269609997893 | 0.0204968557621 | 0.0636422524585 | -0.091926872991 | 0.0892470015351 | -0.0420853381763 | 0.310034841203 | -0.0194210592316 | 0.0389646412519 | 0.265528250326 | -0.0206046896581 | 0.255429408887 | -21.289912637 | 17.4822729563 | -14.4908940234 | 9 | 4.62093036883 | 4.13491552925 | 2.88899078134 | 3.925847703 | 2.26071383572 | 2.41295343236 | 0.287046384183 | -0.610774135075 | -0.340139001803 | -0.397208286823 | -0.440216930872 | -0.714315084499 | -0.0848461724496 | 1.24085739641 | 0.0665274855019 | 0.439881236666 | -0.0670937559537 | -0.0136604509428 | 0.511410512382 | -0.00169022303063 | 0.514431568516 | -5.89931072792 | 21.5841696516 | -5.73101638356 | 10 | 5.13299181346 | 3.91318122844 | 2.28597224936 | 7.40524588586 | 1.77129083987 | 6.65388783501 | -0.17454223581 | 0.514040472777 | 0.088678799894 | -1.00968184077 | -0.0632106486637 | 0.339476957617 | -0.307908061602 | 0.0955961848769 | 0.193886976433 | 0.36518516434 | 0.0835238965873 | -0.0303390342351 | 0.377139938057 | -0.0256393388977 | 0.260374894691 | -22.8725933818 | 14.4054981384 | -22.3040327934 | 11 | 1.04249013834 | -0.00484540685985 | -0.580498231481 | 1.63582812259 | 0.569393667697 | 2.17553040597 | -0.0905326537115 | 0.176330353516 | 0.0666238719344 | -0.0324069966292 | 0.127992849942 | 0.209837841252 | -0.00349128201814 | -0.188816306697 | -0.0754242568141 | 0.302306839599 | 0.0150506296841 | -0.0220404944445 | 0.23082177844 | 0.0247744789679 | 0.279753507169 | -16.8224483527 | 40.4496083194 | 65.1947389718 | 12 | 4.2117293835 | -0.501932474385 | 2.11646975124 | 2.33541068194 | 0.104909939617 | 4.6469228147 | 0.177051617408 | -0.282462631478 | 0.359917095971 | 0.245820500644 | -0.276433329502 | -0.18015715109 | -0.389385339828 | 0.276725493125 | 0.0892596331004 | 0.320546042481 | -0.0187253480008 | 0.0977327631328 | 0.278989503157 | 0.0606702227103 | 0.334649079981 | -17.7417695844 | 44.0740380244 | 97.5524046787 | 13 | 9.30827909999 | -1.84122426248 | 5.56438777269 | 4.35761623362 | -2.38146387972 | 7.26024333182 | -0.0363203185678 | -0.322875794232 | -0.376010236044 | -0.175008246589 | 0.21477714055 | 0.536689211948 | 0.700306457001 | -0.391950424269 | -0.0135120911781 | 0.26438564706 | -0.0413978518818 | 0.0741872556302 | 0.223214225767 | -0.0476939584496 | 0.379580122913 | -21.7879757529 | 16.3712390487 | 75.38624755 | 14 | 3.27572242521 | 0.248632410801 | 0.556280125494 | 3.37876489065 | -1.18051210737 | 3.36134609426 | -0.0121462151103 | -0.115672367131 | -0.45157446725 | 0.132715458977 | 0.000959877954703 | -0.0490352102623 | 0.177383429659 | -0.10445650803 | 0.00994223821312 | 0.312704331302 | 0.0294069641978 | 0.0496786645081 | 0.183871284787 | -0.00406946721267 | 0.267630922931 | -13.7333299235 | 19.9952020265 | 69.0147127866 | 15 | 0.966036032853 | -0.768742588266 | 0.655416866837 | 4.22426976745 | -3.15309959668 | 5.92897191836 | -0.136395384658 | -0.0551555523393 | 0.0108021384844 | -0.0968943179562 | 0.0582549186326 | 0.218985140148 | 0.306452805208 | 0.0733010940513 | 0.0206579766631 | 0.36192937698 | -0.0224919362798 | 0.0607436649249 | 0.271151451282 | -0.0201537532181 | 0.353182201974 | -17.3409615157 | 21.4659721451 | 71.1486844009 | 16 | 0.199712866935 | -0.308445783808 | -0.556736762904 | 0.982387325745 | 1.79551849443 | 3.28557411898 | -0.384890866114 | -0.338889160614 | 0.0669435428924 | 0.348741528197 | 0.456797952503 | 0.322101880285 | 0.73796024512 | -0.32265540318 | 0.0361474737425 | 0.532626923711 | 0.0555617074967 | 0.105947245462 | 0.317035844606 | 0.105033501561 | 0.292145037873 | -18.0011712638 | 18.131685779 | 91.725251823 | 17 | 5.63887517077 | -2.94042675718 | -1.24084801594 | 2.88138883491 | 0.0936041628062 | 4.16372516217 | 0.180840325241 | -0.0199476644542 | 0.984174705862 | 0.0108688983804 | 0.0533944554895 | -0.361212984329 | -0.354849687334 | -0.159305554602 | -0.193625542409 | 0.404622736509 | -0.0812125153167 | 0.0497226902955 | 0.327194436315 | 0.0546072336566 | 0.420505079513 | -24.4196125601 | 43.3923659913 | 107.830392469 | 18 | 4.61434521928 | -3.08218160297 | -2.08973499622 | 2.99899524697 | 1.12053492413 | 3.42626625408 | -0.0692868667904 | -0.102826492775 | -0.203902183029 | 0.130287018455 | -0.0637004606703 | 0.174263968001 | 0.36133818567 | -0.0126397479057 | 0.189407697155 | 0.258940646222 | -0.0486443817559 | -0.00628306860861 | 0.214676719639 | 0.0265029319494 | 0.296713060631 | -29.0831452552 | 30.4990348221 | 103.042028919 | 19 | 3.45491108075 | -1.20544014969 | -2.38121255266 | 2.52050197033 | 1.47593066602 | 3.61405024943 | 0.0660089768587 | -0.061180671153 | -0.1984998751 | 0.0736195791965 | -0.239756299644 | 0.0797897451839 | -0.0343212839438 | 0.0688711998553 | 0.0737785403819 | 0.330768763128 | -0.0019918284161 | -0.00148792396412 | 0.231810589808 | 0.0573109663144 | 0.201652604257 | -26.383181868 | 31.8362841041 | 106.029648565 | 20 | 2.40176119014 | 0.808912481474 | 0.0179050461532 | 2.57994266928 | 0.318758237084 | 1.41286575902 | 0.398881421602 | -0.936855611994 | 0.256364666823 | 0.17338516637 | 0.0684132191012 | -0.58139543765 | 0.195007067849 | 0.612988268886 | -0.309944543244 | 0.442660000232 | -0.00499766906949 | -0.0140543375108 | 0.862609863336 | -0.180525661975 | 0.338360201613 | 9.26753590119 | 24.1376583807 | 48.8444730789 | 21 | 4.7635874428 | -0.745979528931 | 0.484295935764 | 4.3026006005 | 0.425384179341 | 2.58510657804 | 0.225264972131 | -0.492999125738 | 0.505501282828 | 0.182194946017 | 0.0918137889466 | -1.001109376 | -0.640517283436 | 1.03845530246 | -0.207512547818 | 0.581930464957 | -0.246890675352 | 0.172818292368 | 0.968179790621 | -0.180543903301 | 0.702512641241 | 18.4215683985 | 34.8091679728 | 47.3238256305 | 22 | 1.31731352103 | 0.100204600702 | -1.89493487018 | 0.28821873308 | 0.0634647147692 | 2.87433257535 | 0.592438931713 | -0.199784284864 | -0.0460080269601 | 0.0142349135643 | 0.130088429379 | -1.30154662583 | -0.237641846999 | 0.724763562838 | -0.132476791938 | 0.607322874968 | -0.321540123228 | 0.0847153822749 | 1.87770791386 | -0.582216144271 | 1.29543409781 | 29.0519313376 | 34.547382599 | 57.6911465497 | 23 | 8.03135158695 | 2.28858890494 | 1.96139860735 | 5.374198704 | 1.21814975173 | 5.07067328404 | 0.031475160315 | 0.367080867058 | 1.01726947479 | -0.1138360245 | 0.291552687545 | -0.438341517656 | -0.543910334733 | 0.617053369042 | -0.428034076369 | 0.49397413035 | -0.0364722676683 | 0.0594086694772 | 0.360245673745 | -0.143581458735 | 0.317685893857 | -0.572455176228 | 33.4373304553 | 52.1272716747 | 24 | 5.26370324155 | -0.0407625069234 | -0.820774987015 | 2.36655784483 | -0.0324497569347 | 2.68976333984 | 0.209689124538 | 0.189173067373 | -0.813146877567 | 0.025624140807 | -0.152111649701 | -0.119062428451 | 0.373736855356 | 0.604483183366 | -0.147402580571 | 0.56982572538 | 0.116890316438 | -0.0476451322634 | 0.52771734992 | -0.0768144285984 | 0.258814202181 | 2.23051817863 | 19.7655069165 | 45.8100388324 | 25 | 4.10199295736 | 0.48745264707 | -0.69384582262 | 4.61112305536 | 0.288446756358 | 3.2048239062 | 0.278854387195 | -0.409787978823 | -0.716287533101 | 0.370329849436 | -0.263464832181 | -1.22171016444 | 0.734656353904 | 1.68533438968 | -0.0408199915458 | 0.732108249552 | 0.166587741306 | -0.196569311377 | 1.32439308452 | -0.0940925499303 | 0.606518563487 | 19.9778683105 | 15.5157034521 | 47.549683629 | 26 | 2.14259960531 | 0.245677550245 | 0.00566078195795 | 0.330596513447 | -0.143879077959 | 0.0861750522552 | -0.0920274165958 | -0.234939103172 | 0.130645451507 | 0.088831836919 | -0.0261249666829 | -0.480685086284 | 0.0560380168185 | 0.0486595050974 | 0.0275018790324 | 0.394174055859 | 0.504165791215 | 0.0085755187018 | 2.04744439153 | -0.186264486336 | 1.1117163817 | 34.6573603165 | 14.0458692082 | 44.9317991609 | 27 | 3.37911673518 | 2.01327704887 | -2.13911238122 | 3.42657558333 | 0.332836377288 | 2.51293961909 | 0.34178230212 | -0.485890810421 | -0.89517590995 | 0.260409963865 | -0.214798876161 | -1.33810969958 | 0.271346690188 | 0.628556781303 | -0.28090248296 | 0.482860519215 | 0.194955993106 | -0.219107563092 | 1.78575725232 | -0.302960039037 | 1.09948666828 | 27.0471443268 | 12.7522807266 | 36.464440883 | 28 | 5.07550471743 | -1.46342671892 | 1.02887102075 | 2.43995460309 | 0.315849045613 | 3.89152448755 | 0.630733674672 | -0.302862915195 | -0.14776810476 | -0.106621778122 | -0.169845587551 | -0.327882090301 | 0.781889537425 | 0.457132343195 | -0.362452683447 | 0.60775032178 | 0.142456538409 | 0.0742970473888 | 0.388108666689 | -0.0788743333837 | 0.290586958539 | 1.72165970022 | 13.6512888653 | 39.2351681682 |
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精密化 TLSグループ | Refine-ID: X-RAY DIFFRACTION ID | Refine TLS-ID | Selection details | Auth asym-ID | Label asym-ID | Auth seq-ID | Label seq-ID |
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1 | 1 | chain 'H' and (resid 1 through 119 )HA1 - 119 | 1 - 119 | 2 | 2 | chain 'H' and (resid 120 through 223 )HA120 - 223 | 120 - 217 | 3 | 3 | chain 'L' and (resid 0 through 59 )LB0 - 59 | 1 - 60 | 4 | 4 | chain 'L' and (resid 60 through 73 )LB60 - 73 | 61 - 74 | 5 | 5 | chain 'L' and (resid 74 through 100 )LB74 - 100 | 75 - 101 | 6 | 6 | chain 'L' and (resid 101 through 111 )LB101 - 111 | 102 - 112 | 7 | 7 | chain 'L' and (resid 112 through 126 )LB112 - 126 | 113 - 127 | 8 | 8 | chain 'L' and (resid 127 through 161 )LB127 - 161 | 128 - 162 | 9 | 9 | chain 'L' and (resid 162 through 172 )LB162 - 172 | 163 - 173 | 10 | 10 | chain 'L' and (resid 173 through 212 )LB173 - 212 | 174 - 213 | 11 | 11 | chain 'A' and (resid 1 through 119 )AC1 - 119 | 1 - 119 | 12 | 12 | chain ' | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
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