PDB-9c1s: Fab F946 in complex with OspC type A

ID or keywords:

Entry

Database: PDB / ID: 9c1s

Title

Fab F946 in complex with OspC type A

Components

F946 Fab Heavy Chain
F946 Fab Light Chain
Outer surface protein C

Keywords

IMMUNE SYSTEM/ANTIGEN / Fab-antigen complex / IMMUNE SYSTEM / IMMUNE SYSTEM-ANTIGEN complex

Function / homology

external side of cell outer membrane / Lipoprotein, OspC-type / Outer surface protein C-like superfamily / Lipoprotein / Prokaryotic membrane lipoprotein lipid attachment site profile. / cell surface / membrane / Outer surface protein C

Function and homology information

Biological species

Homo sapiens (human)

Borreliella burgdorferi (Lyme disease spirochete)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 2.6 Å

Authors

Rudolph, M.J. / Mantis, N.

Funding support

United States, 1items

Citation

Journal: To Be Published
Title: Fab F946 in complex with OspC type A
Authors: Rudolph, M.J. / Mantis, N.

History

Deposition	May 29, 2024	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Aug 27, 2025	Provider: repository / Type: Initial release

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	9c1s.cif.gz	555.7 KB	Display	PDBx/mmCIF format
PDB format	pdb9c1s.ent.gz	381.5 KB	Display	PDB format
PDBx/mmJSON format	9c1s.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Summary document	9c1s_validation.pdf.gz	462.9 KB	Display	wwPDB validaton report
Full document	9c1s_full_validation.pdf.gz	475 KB	Display
Data in XML	9c1s_validation.xml.gz	48 KB	Display
Data in CIF	9c1s_validation.cif.gz	63.3 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/c1/9c1s ftp://data.pdbj.org/pub/pdb/validation_reports/c1/9c1s	HTTPS FTP

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Related structure data

Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#21 - Sep 2001 Antibodies similarity (1) #256 - Apr 2021 SARS-CoV-2 Spike and Antibodies similarity (1) #261 - Sep 2021 DNA-Sequencing Nanopores similarity (1) #22 - Oct 2001 Photosystem I similarity (1) #262 - Oct 2021 Fifty Years of Open Access to PDB Structures similarity (1) #29 - May 2002 Penicillin-binding Proteins similarity (1) #95 - Nov 2007 Multidrug Resistance Transporters similarity (1)

PDB pages

PDBj /

wwPDB /

NCBI

Related items in Molecule of the Month

#21 - Sep 2001
Antibodies
similarity (1)
#256 - Apr 2021
SARS-CoV-2 Spike and Antibodies
similarity (1)
#261 - Sep 2021
DNA-Sequencing Nanopores
similarity (1)
#22 - Oct 2001
Photosystem I
similarity (1)
#262 - Oct 2021
Fifty Years of Open Access to PDB Structures
similarity (1)
#29 - May 2002
Penicillin-binding Proteins
similarity (1)
#95 - Nov 2007
Multidrug Resistance Transporters
similarity (1)

Deposited unit

H: F946 Fab Heavy Chain

L: F946 Fab Light Chain

A: F946 Fab Heavy Chain

B: F946 Fab Light Chain

C: Outer surface protein C

D: Outer surface protein C

131 kDa, 6 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author&software

Unit cell

Length a, b, c (Å)	79.815, 95.141, 182.968
Angle α, β, γ (deg.)	90.000, 90.000, 90.000
Int Tables number	19
Space group name H-M	P2₁2₁2₁
Space group name Hall	P2ac2ab

#1: Antibody	F946 Fab Heavy Chain Mass: 24591.562 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Cricetulus griseus (Chinese hamster)
#2: Antibody	F946 Fab Light Chain Mass: 23429.057 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Cricetulus griseus (Chinese hamster)
#3: Protein	Outer surface protein C / PC Mass: 17528.031 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Borreliella burgdorferi (Lyme disease spirochete) Strain: B31 / Gene: ospC, BB_B19 / Production host: Escherichia coli B (bacteria) / References: UniProt: Q07337
#4: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 233 / Source method: isolated from a natural source / Formula: H₂O
Has protein modification	Y

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.65 Å³/Da / Density % sol: 53.58 %
Crystal grow	Temperature: 295 K / Method: vapor diffusion, sitting drop / Details: 200 mM Ammonium nitrate and 20% PEG 3350

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Data collection

Diffraction	Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: NSLS-II / Beamline: 19-ID / Wavelength: 0.979 Å
Detector	Type: DECTRIS EIGER2 S 9M / Detector: PIXEL / Date: Oct 5, 2023
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.979 Å / Relative weight: 1
Reflection	Resolution: 2.55→50 Å / Num. obs: 42767 / % possible obs: 99.9 % / Redundancy: 11.5 % / B_iso Wilson estimate: 38.5 Å² / CC_1/2: 0.997 / Net I/σ(I): 10
Reflection shell	Resolution: 2.55→2.59 Å / Num. unique obs: 1909 / CC_1/2: 0.71 / % possible all: 91.1

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Processing

Software

Name	Version	Classification
PHENIX	1.20.1_4487	refinement
HKL-2000		data reduction
HKL-2000		data scaling
PHASER		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT / Resolution: 2.6→47.57 Å / SU ML: 0.3452 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 25.2688
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2

	R_factor	Num. reflection	% reflection
R_free	0.2508	2083	4.95 %
R_work	0.1958	39993	-
obs	0.1985	42076	96.22 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso mean: 54.04 Å²

Refinement step

Cycle: LAST / Resolution: 2.6→47.57 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	8960	0	0	233	9193

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.002	9133
X-RAY DIFFRACTION	f_angle_d	0.5609	12382
X-RAY DIFFRACTION	f_chiral_restr	0.0399	1435
X-RAY DIFFRACTION	f_plane_restr	0.0039	1563
X-RAY DIFFRACTION	f_dihedral_angle_d	5.1807	1236

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.6-2.66	0.3305	93	0.2516	1688	X-RAY DIFFRACTION	61.5
2.66-2.73	0.3115	130	0.2621	2479	X-RAY DIFFRACTION	92
2.73-2.8	0.3321	131	0.2669	2614	X-RAY DIFFRACTION	95.02
2.8-2.88	0.3248	126	0.2585	2632	X-RAY DIFFRACTION	96.53
2.88-2.97	0.3627	136	0.2588	2721	X-RAY DIFFRACTION	98.82
2.97-3.08	0.3428	125	0.242	2749	X-RAY DIFFRACTION	99.93
3.08-3.2	0.3084	132	0.2283	2768	X-RAY DIFFRACTION	100
3.2-3.35	0.2737	155	0.2159	2727	X-RAY DIFFRACTION	99.97
3.35-3.53	0.265	174	0.2136	2721	X-RAY DIFFRACTION	100
3.53-3.75	0.2261	160	0.1978	2742	X-RAY DIFFRACTION	100
3.75-4.04	0.2463	163	0.1793	2750	X-RAY DIFFRACTION	100
4.04-4.44	0.235	114	0.1541	2825	X-RAY DIFFRACTION	100
4.44-5.08	0.1774	146	0.1438	2784	X-RAY DIFFRACTION	100
5.08-6.4	0.2114	147	0.1728	2844	X-RAY DIFFRACTION	100
6.4-47.57	0.1974	151	0.1726	2949	X-RAY DIFFRACTION	99.01

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	2.19890707179	0.29050053278	0.421458475989	3.20600175207	-0.116599437795	2.4570457573	-0.0701084740276	-0.102774851047	-0.218696932661	0.000644702870596	0.235844141428	0.533944373284	0.148266128289	-0.263473939688	-0.105014962778	0.283862828076	-0.027804059355	0.00466457433165	0.233741156006	0.0134555310005	0.321665701787	-11.6625195209	6.43445719642	22.9382377989
2	3.58820484924	1.86794771553	-1.13953897119	3.79637176884	-0.165900862399	5.19710105357	0.164197038702	0.27523906403	-0.366170821353	-0.30781614307	0.106424220058	-0.234096228175	0.579060940186	0.569926053253	-0.130277219809	0.413416202134	0.135781508197	-0.145724695773	0.371944105753	-0.135886328387	0.429845675079	-9.72532282147	3.2228355602	-10.8487098537
3	2.44320900943	-0.481023308393	-0.580044741072	3.74950231138	0.222511263062	5.03921489129	-0.249672570998	0.0707898625387	0.160660470548	-0.00603942597215	0.214280394305	0.394626007574	-0.092552144443	-0.195355216245	0.00669486968847	0.298630754584	-0.0151528818847	-0.0800602148406	0.199893754409	0.0174813777006	0.343789038118	-8.92219863195	27.1532975232	16.6916439424
4	5.30244567435	1.19849082821	-4.57167897625	0.84996515718	-0.425930982069	6.27588226457	0.326148715269	0.481990004941	0.470031028388	0.0412375531265	-0.0961972001712	0.589842439608	-1.46194125254	-0.416827967258	-0.251357427747	0.540413530472	0.0821270271948	-0.0619505750796	0.217093317367	-0.0546162665869	0.465323470485	-9.40395784935	33.3800731317	20.0855324436
5	5.52373188124	3.88217795329	-3.30785538498	3.95781319402	-3.21949882857	7.78101229643	-0.151267216713	-0.0189845640385	0.170799945098	-0.344037939323	0.173303772458	0.207456736301	0.322872693579	-0.124293519032	0.00870014272416	0.349588470487	0.0692540428544	-0.0811134223816	0.19965341024	-0.0457774363636	0.337496332343	-10.5079918376	23.6527567101	16.2911578786
6	1.61946394747	-0.329482460683	0.635246653845	2.72393701446	3.55415887057	5.35545164694	-0.0933910652434	0.489175414958	0.247169141345	-0.615445295804	0.827531720345	-0.284498278183	0.00404444796128	-0.195061841482	-0.607385632132	0.464672636959	-0.175634337794	-0.0326173532271	0.438786510204	0.0422479654268	0.240600194512	-8.40494015307	29.1791014488	-5.32261696244
7	4.67376984506	1.58792956114	1.52042049393	4.6100798222	0.0783168004997	3.59068316372	0.250589214251	0.617286332123	-0.834518806344	-0.2409367926	0.351605139047	-0.492114359669	0.456501964784	0.361717286419	-0.563900753517	0.493260763951	0.0381113373146	-0.0328735779819	0.507248083956	-0.155046772968	0.444012954575	-17.5981886155	4.98988448028	-21.1480367969
8	3.73535839285	0.793600512857	1.88209650554	2.23694017638	1.22312727939	2.38853443003	0.0591042318024	0.295821020114	-0.118370433729	0.0269609997893	0.0204968557621	0.0636422524585	-0.091926872991	0.0892470015351	-0.0420853381763	0.310034841203	-0.0194210592316	0.0389646412519	0.265528250326	-0.0206046896581	0.255429408887	-21.289912637	17.4822729563	-14.4908940234
9	4.62093036883	4.13491552925	2.88899078134	3.925847703	2.26071383572	2.41295343236	0.287046384183	-0.610774135075	-0.340139001803	-0.397208286823	-0.440216930872	-0.714315084499	-0.0848461724496	1.24085739641	0.0665274855019	0.439881236666	-0.0670937559537	-0.0136604509428	0.511410512382	-0.00169022303063	0.514431568516	-5.89931072792	21.5841696516	-5.73101638356
10	5.13299181346	3.91318122844	2.28597224936	7.40524588586	1.77129083987	6.65388783501	-0.17454223581	0.514040472777	0.088678799894	-1.00968184077	-0.0632106486637	0.339476957617	-0.307908061602	0.0955961848769	0.193886976433	0.36518516434	0.0835238965873	-0.0303390342351	0.377139938057	-0.0256393388977	0.260374894691	-22.8725933818	14.4054981384	-22.3040327934
11	1.04249013834	-0.00484540685985	-0.580498231481	1.63582812259	0.569393667697	2.17553040597	-0.0905326537115	0.176330353516	0.0666238719344	-0.0324069966292	0.127992849942	0.209837841252	-0.00349128201814	-0.188816306697	-0.0754242568141	0.302306839599	0.0150506296841	-0.0220404944445	0.23082177844	0.0247744789679	0.279753507169	-16.8224483527	40.4496083194	65.1947389718
12	4.2117293835	-0.501932474385	2.11646975124	2.33541068194	0.104909939617	4.6469228147	0.177051617408	-0.282462631478	0.359917095971	0.245820500644	-0.276433329502	-0.18015715109	-0.389385339828	0.276725493125	0.0892596331004	0.320546042481	-0.0187253480008	0.0977327631328	0.278989503157	0.0606702227103	0.334649079981	-17.7417695844	44.0740380244	97.5524046787
13	9.30827909999	-1.84122426248	5.56438777269	4.35761623362	-2.38146387972	7.26024333182	-0.0363203185678	-0.322875794232	-0.376010236044	-0.175008246589	0.21477714055	0.536689211948	0.700306457001	-0.391950424269	-0.0135120911781	0.26438564706	-0.0413978518818	0.0741872556302	0.223214225767	-0.0476939584496	0.379580122913	-21.7879757529	16.3712390487	75.38624755
14	3.27572242521	0.248632410801	0.556280125494	3.37876489065	-1.18051210737	3.36134609426	-0.0121462151103	-0.115672367131	-0.45157446725	0.132715458977	0.000959877954703	-0.0490352102623	0.177383429659	-0.10445650803	0.00994223821312	0.312704331302	0.0294069641978	0.0496786645081	0.183871284787	-0.00406946721267	0.267630922931	-13.7333299235	19.9952020265	69.0147127866
15	0.966036032853	-0.768742588266	0.655416866837	4.22426976745	-3.15309959668	5.92897191836	-0.136395384658	-0.0551555523393	0.0108021384844	-0.0968943179562	0.0582549186326	0.218985140148	0.306452805208	0.0733010940513	0.0206579766631	0.36192937698	-0.0224919362798	0.0607436649249	0.271151451282	-0.0201537532181	0.353182201974	-17.3409615157	21.4659721451	71.1486844009
16	0.199712866935	-0.308445783808	-0.556736762904	0.982387325745	1.79551849443	3.28557411898	-0.384890866114	-0.338889160614	0.0669435428924	0.348741528197	0.456797952503	0.322101880285	0.73796024512	-0.32265540318	0.0361474737425	0.532626923711	0.0555617074967	0.105947245462	0.317035844606	0.105033501561	0.292145037873	-18.0011712638	18.131685779	91.725251823
17	5.63887517077	-2.94042675718	-1.24084801594	2.88138883491	0.0936041628062	4.16372516217	0.180840325241	-0.0199476644542	0.984174705862	0.0108688983804	0.0533944554895	-0.361212984329	-0.354849687334	-0.159305554602	-0.193625542409	0.404622736509	-0.0812125153167	0.0497226902955	0.327194436315	0.0546072336566	0.420505079513	-24.4196125601	43.3923659913	107.830392469
18	4.61434521928	-3.08218160297	-2.08973499622	2.99899524697	1.12053492413	3.42626625408	-0.0692868667904	-0.102826492775	-0.203902183029	0.130287018455	-0.0637004606703	0.174263968001	0.36133818567	-0.0126397479057	0.189407697155	0.258940646222	-0.0486443817559	-0.00628306860861	0.214676719639	0.0265029319494	0.296713060631	-29.0831452552	30.4990348221	103.042028919
19	3.45491108075	-1.20544014969	-2.38121255266	2.52050197033	1.47593066602	3.61405024943	0.0660089768587	-0.061180671153	-0.1984998751	0.0736195791965	-0.239756299644	0.0797897451839	-0.0343212839438	0.0688711998553	0.0737785403819	0.330768763128	-0.0019918284161	-0.00148792396412	0.231810589808	0.0573109663144	0.201652604257	-26.383181868	31.8362841041	106.029648565
20	2.40176119014	0.808912481474	0.0179050461532	2.57994266928	0.318758237084	1.41286575902	0.398881421602	-0.936855611994	0.256364666823	0.17338516637	0.0684132191012	-0.58139543765	0.195007067849	0.612988268886	-0.309944543244	0.442660000232	-0.00499766906949	-0.0140543375108	0.862609863336	-0.180525661975	0.338360201613	9.26753590119	24.1376583807	48.8444730789
21	4.7635874428	-0.745979528931	0.484295935764	4.3026006005	0.425384179341	2.58510657804	0.225264972131	-0.492999125738	0.505501282828	0.182194946017	0.0918137889466	-1.001109376	-0.640517283436	1.03845530246	-0.207512547818	0.581930464957	-0.246890675352	0.172818292368	0.968179790621	-0.180543903301	0.702512641241	18.4215683985	34.8091679728	47.3238256305
22	1.31731352103	0.100204600702	-1.89493487018	0.28821873308	0.0634647147692	2.87433257535	0.592438931713	-0.199784284864	-0.0460080269601	0.0142349135643	0.130088429379	-1.30154662583	-0.237641846999	0.724763562838	-0.132476791938	0.607322874968	-0.321540123228	0.0847153822749	1.87770791386	-0.582216144271	1.29543409781	29.0519313376	34.547382599	57.6911465497
23	8.03135158695	2.28858890494	1.96139860735	5.374198704	1.21814975173	5.07067328404	0.031475160315	0.367080867058	1.01726947479	-0.1138360245	0.291552687545	-0.438341517656	-0.543910334733	0.617053369042	-0.428034076369	0.49397413035	-0.0364722676683	0.0594086694772	0.360245673745	-0.143581458735	0.317685893857	-0.572455176228	33.4373304553	52.1272716747
24	5.26370324155	-0.0407625069234	-0.820774987015	2.36655784483	-0.0324497569347	2.68976333984	0.209689124538	0.189173067373	-0.813146877567	0.025624140807	-0.152111649701	-0.119062428451	0.373736855356	0.604483183366	-0.147402580571	0.56982572538	0.116890316438	-0.0476451322634	0.52771734992	-0.0768144285984	0.258814202181	2.23051817863	19.7655069165	45.8100388324
25	4.10199295736	0.48745264707	-0.69384582262	4.61112305536	0.288446756358	3.2048239062	0.278854387195	-0.409787978823	-0.716287533101	0.370329849436	-0.263464832181	-1.22171016444	0.734656353904	1.68533438968	-0.0408199915458	0.732108249552	0.166587741306	-0.196569311377	1.32439308452	-0.0940925499303	0.606518563487	19.9778683105	15.5157034521	47.549683629
26	2.14259960531	0.245677550245	0.00566078195795	0.330596513447	-0.143879077959	0.0861750522552	-0.0920274165958	-0.234939103172	0.130645451507	0.088831836919	-0.0261249666829	-0.480685086284	0.0560380168185	0.0486595050974	0.0275018790324	0.394174055859	0.504165791215	0.0085755187018	2.04744439153	-0.186264486336	1.1117163817	34.6573603165	14.0458692082	44.9317991609
27	3.37911673518	2.01327704887	-2.13911238122	3.42657558333	0.332836377288	2.51293961909	0.34178230212	-0.485890810421	-0.89517590995	0.260409963865	-0.214798876161	-1.33810969958	0.271346690188	0.628556781303	-0.28090248296	0.482860519215	0.194955993106	-0.219107563092	1.78575725232	-0.302960039037	1.09948666828	27.0471443268	12.7522807266	36.464440883
28	5.07550471743	-1.46342671892	1.02887102075	2.43995460309	0.315849045613	3.89152448755	0.630733674672	-0.302862915195	-0.14776810476	-0.106621778122	-0.169845587551	-0.327882090301	0.781889537425	0.457132343195	-0.362452683447	0.60775032178	0.142456538409	0.0742970473888	0.388108666689	-0.0788743333837	0.290586958539	1.72165970022	13.6512888653	39.2351681682

Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

ID	Refine TLS-ID	Selection details	Auth asym-ID	Label asym-ID	Auth seq-ID	Label seq-ID
1	1	chain 'H' and (resid 1 through 119 )	H	A	1 - 119	1 - 119
2	2	chain 'H' and (resid 120 through 223 )	H	A	120 - 223	120 - 217
3	3	chain 'L' and (resid 0 through 59 )	L	B	0 - 59	1 - 60
4	4	chain 'L' and (resid 60 through 73 )	L	B	60 - 73	61 - 74
5	5	chain 'L' and (resid 74 through 100 )	L	B	74 - 100	75 - 101
6	6	chain 'L' and (resid 101 through 111 )	L	B	101 - 111	102 - 112
7	7	chain 'L' and (resid 112 through 126 )	L	B	112 - 126	113 - 127
8	8	chain 'L' and (resid 127 through 161 )	L	B	127 - 161	128 - 162
9	9	chain 'L' and (resid 162 through 172 )	L	B	162 - 172	163 - 173
10	10	chain 'L' and (resid 173 through 212 )	L	B	173 - 212	174 - 213
11	11	chain 'A' and (resid 1 through 119 )	A	C	1 - 119	1 - 119
12	12	chain 'A' and (resid 120 through 225 )	A	C	120 - 225	120 - 221
13	13	chain 'B' and (resid -1 through 16 )	B	D	-1 - 16	1 - 18
14	14	chain 'B' and (resid 17 through 67 )	B	D	17 - 67	19 - 69
15	15	chain 'B' and (resid 68 through 99 )	B	D	68 - 99	70 - 101
16	16	chain 'B' and (resid 100 through 111 )	B	D	100 - 111	102 - 113
17	17	chain 'B' and (resid 112 through 126 )	B	D	112 - 126	114 - 128
18	18	chain 'B' and (resid 127 through 161 )	B	D	127 - 161	129 - 163
19	19	chain 'B' and (resid 162 through 210 )	B	D	162 - 210	164 - 212
20	20	chain 'C' and (resid 41 through 86 )	C	E	41 - 86	1 - 46
21	21	chain 'C' and (resid 87 through 140 )	C	E	87 - 140	47 - 100
22	22	chain 'C' and (resid 141 through 170 )	C	E	141 - 170	101 - 127
23	23	chain 'C' and (resid 171 through 201 )	C	E	171 - 201	128 - 158
24	24	chain 'D' and (resid 40 through 75 )	D	F	40 - 75	1 - 36
25	25	chain 'D' and (resid 76 through 140 )	D	F	76 - 140	37 - 98
26	26	chain 'D' and (resid 141 through 158 )	D	F	141 - 158	99 - 116
27	27	chain 'D' and (resid 159 through 169 )	D	F	159 - 169	117 - 127
28	28	chain 'D' and (resid 170 through 201 )	D	F	170 - 201	128 - 159

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