endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters / micrococcal nuclease / nucleic acid binding / extracellular region / membrane / metal ion binding Similarity search - Function
Thermonuclease family signature 1. / Thermonuclease active site / Thermonuclease family signature 2. / Staphylococcal nuclease (SNase-like), OB-fold / Staphylococcal nuclease homologue / Thermonuclease domain profile. / Staphylococcal nuclease homologues / SNase-like, OB-fold superfamily Similarity search - Domain/homology
Monochromator: M / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 1.54184 Å / Relative weight: 1
Reflection
Resolution: 1.95→23.21 Å / Num. obs: 22934 / % possible obs: 99.8 % / Redundancy: 9.6 % / Biso Wilson estimate: 25.76 Å2 / Rmerge(I) obs: 0.015 / Rsym value: 0.043 / Net I/σ(I): 104.39
Reflection shell
Resolution: 1.95→2.02 Å / Redundancy: 7.2 % / Rmerge(I) obs: 0.193 / Mean I/σ(I) obs: 5.88 / Num. unique obs: 2297 / Rsym value: 0.242 / % possible all: 99.9
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Processing
Software
Name
Version
Classification
REFMAC
5.8.0425
refinement
CrysalisPro
1.171.42.95a
datascaling
CrysalisPro
1.171.42.95a
datareduction
PHASER
2.8.3
phasing
Refinement
Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.95→21.76 Å / Cor.coef. Fo:Fc: 0.948 / Cor.coef. Fo:Fc free: 0.922 / SU B: 9.143 / SU ML: 0.146 / Cross valid method: THROUGHOUT / ESU R: 0.187 / ESU R Free: 0.172 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.26094
1137
5 %
RANDOM
Rwork
0.21065
-
-
-
obs
0.21313
21748
99.86 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK