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- PDB-9bf9: Human LAG-3-HLA-DR1 complex -

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ID or keywords:

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Basic information

Entry
Database: PDB / ID: 9bf9
TitleHuman LAG-3-HLA-DR1 complex
Components
  • (HLA class II histocompatibility ...) x 2
  • Lymphocyte activation gene 3 protein
  • Membrane protein
KeywordsIMMUNE SYSTEM / Immune receptor complex Class II Human Leucocyte Antigen Lymphocyte activation Gene-3 IMMUNE SYSTEM
Function / homology
Function and homology information


Maturation of protein M / plasmacytoid dendritic cell activation / negative regulation of regulatory T cell differentiation / SARS-CoV-2 modulates autophagy / myeloid dendritic cell antigen processing and presentation / antigen processing and presentation of endogenous peptide antigen via MHC class II / autolysosome membrane / regulation of T-helper cell differentiation / positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation / positive regulation of natural killer cell mediated cytotoxicity ...Maturation of protein M / plasmacytoid dendritic cell activation / negative regulation of regulatory T cell differentiation / SARS-CoV-2 modulates autophagy / myeloid dendritic cell antigen processing and presentation / antigen processing and presentation of endogenous peptide antigen via MHC class II / autolysosome membrane / regulation of T-helper cell differentiation / positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation / positive regulation of natural killer cell mediated cytotoxicity / MHC class II receptor activity / positive regulation of CD4-positive, alpha-beta T cell activation / MHC class II protein binding / antigen processing and presentation of peptide or polysaccharide antigen via MHC class II / positive regulation of memory T cell differentiation / negative regulation of interleukin-2 production / natural killer cell mediated cytotoxicity / transport vesicle membrane / Translocation of ZAP-70 to Immunological synapse / Phosphorylation of CD3 and TCR zeta chains / host cell Golgi membrane / polysaccharide binding / Generation of second messenger molecules / immunological synapse / Co-inhibition by PD-1 / CD28 dependent PI3K/Akt signaling / regulation of immune response / SARS-CoV-2 targets host intracellular signalling and regulatory pathways / T cell receptor binding / antigen binding / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity / MHC class II antigen presentation / protein sequestering activity / T cell activation / trans-Golgi network membrane / VEGFR2 mediated vascular permeability / lumenal side of endoplasmic reticulum membrane / peptide antigen assembly with MHC class II protein complex / MHC class II protein complex / clathrin-coated endocytic vesicle membrane / ER to Golgi transport vesicle membrane / antigen processing and presentation of exogenous peptide antigen via MHC class II / positive regulation of immune response / peptide antigen binding / positive regulation of T cell mediated cytotoxicity / positive regulation of T cell activation / cognition / Interferon gamma signaling / MHC class II protein complex binding / endocytic vesicle membrane / late endosome membrane / transmembrane signaling receptor activity / Downstream TCR signaling / PIP3 activates AKT signaling / TRAF3-dependent IRF activation pathway / early endosome membrane / adaptive immune response / Translation of Structural Proteins / Virion Assembly and Release / Induction of Cell-Cell Fusion / structural constituent of virion / Attachment and Entry / lysosome / cell surface receptor signaling pathway / endosome membrane / immune response / Golgi membrane / external side of plasma membrane / lysosomal membrane / innate immune response / viral envelope / SARS-CoV-2 activates/modulates innate and adaptive immune responses / virion membrane / cell surface / extracellular exosome / extracellular region / identical protein binding / plasma membrane
Similarity search - Function
M matrix/glycoprotein, SARS-CoV-like / M matrix/glycoprotein, coronavirus / Coronavirus M matrix/glycoprotein / Coronavirus membrane (Cov-M) protein profile. / Interleukin-1 receptor family / MHC class II, beta chain, N-terminal / Class II histocompatibility antigen, beta domain / Class II histocompatibility antigen, beta domain / MHC class II, alpha chain, N-terminal / Class II histocompatibility antigen, alpha domain ...M matrix/glycoprotein, SARS-CoV-like / M matrix/glycoprotein, coronavirus / Coronavirus M matrix/glycoprotein / Coronavirus membrane (Cov-M) protein profile. / Interleukin-1 receptor family / MHC class II, beta chain, N-terminal / Class II histocompatibility antigen, beta domain / Class II histocompatibility antigen, beta domain / MHC class II, alpha chain, N-terminal / Class II histocompatibility antigen, alpha domain / Class II histocompatibility antigen, alpha domain / MHC class II, alpha/beta chain, N-terminal / MHC classes I/II-like antigen recognition protein / : / Immunoglobulin subtype / Immunoglobulin / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold
Similarity search - Domain/homology
DI(HYDROXYETHYL)ETHER / HLA class II histocompatibility antigen DR beta chain / HLA class II histocompatibility antigen, DR alpha chain / Membrane protein / Lymphocyte activation gene 3 protein
Similarity search - Component
Biological speciesHomo sapiens (human)
Severe acute respiratory syndrome coronavirus 2
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.4 Å
AuthorsPetersen, J. / Rossjohn, J.
Funding support Australia, 1items
OrganizationGrant numberCountry
Australian Research Council (ARC)ARC LP190101290 Australia
CitationJournal: Sci Immunol / Year: 2024
Title: Crystal structure of the human LAG-3-HLA-DR1-peptide complex.
Authors: Petersen, J. / Llerena, C. / Golzarroshan, B. / Faoro, C. / Triebel, F. / Rossjohn, J.
History
DepositionApr 17, 2024Deposition site: RCSB / Processing site: RCSB
Revision 1.0Dec 25, 2024Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: HLA class II histocompatibility antigen, DR alpha chain
B: HLA class II histocompatibility antigen DR beta chain
G: Membrane protein
D: Lymphocyte activation gene 3 protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)94,21910
Polymers92,6934
Non-polymers1,5266
Water00
1
A: HLA class II histocompatibility antigen, DR alpha chain
B: HLA class II histocompatibility antigen DR beta chain
G: Membrane protein
D: Lymphocyte activation gene 3 protein
hetero molecules

A: HLA class II histocompatibility antigen, DR alpha chain
B: HLA class II histocompatibility antigen DR beta chain
G: Membrane protein
D: Lymphocyte activation gene 3 protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)188,43920
Polymers185,3868
Non-polymers3,05312
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
point symmetry operation1
Unit cell
Length a, b, c (Å)97.714, 148.067, 85.586
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number18
Space group name H-MP21212
Space group name HallP22ab
Symmetry operation#1: x,y,z
#2: x+1/2,-y+1/2,-z
#3: -x+1/2,y+1/2,-z
#4: -x,-y,z

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Components

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HLA class II histocompatibility ... , 2 types, 2 molecules AB

#1: Protein HLA class II histocompatibility antigen, DR alpha chain / MHC class II antigen DRA


Mass: 21919.594 Da / Num. of mol.: 1 / Fragment: UNP residues 30-206
Source method: isolated from a genetically manipulated source
Details: HLA-DRA1*0101 with terminal / Source: (gene. exp.) Homo sapiens (human) / Gene: HLA-DRA, HLA-DRA1 / Cell line (production host): HEK293F (GNTI-) / Production host: Homo sapiens (human) / References: UniProt: P01903
#2: Protein HLA class II histocompatibility antigen DR beta chain / HLA-DRB1 protein / MHC class II antigen


Mass: 24033.531 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: HLA-DRB1 / Cell line (production host): HEK293F (GNTI-) / Production host: Homo sapiens (human) / References: UniProt: D7RIG0

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Protein/peptide / Protein , 2 types, 2 molecules GD

#3: Protein/peptide Membrane protein / M / E1 glycoprotein / Matrix glycoprotein / Membrane glycoprotein


Mass: 1401.569 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2
Cell line (production host): HEK293F (GNTI-) / Production host: Homo sapiens (human) / References: UniProt: P0DTC5
#4: Protein Lymphocyte activation gene 3 protein / LAG-3


Mass: 45338.270 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: LAG3, FDC / Cell line (production host): HEK293F / Production host: Homo sapiens (human) / References: UniProt: P18627

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Sugars , 2 types, 2 molecules

#5: Polysaccharide alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2- ...alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 910.823 Da / Num. of mol.: 1 / Source method: obtained synthetically
DescriptorTypeProgram
DManpa1-3[DManpa1-6]DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,5,4/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3-3/a4-b1_b4-c1_c3-d1_c6-e1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{}[(6+1)][a-D-Manp]{}}}}LINUCSPDB-CARE
#7: Sugar ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 2 types, 4 molecules

#6: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: SO4
#8: Chemical ChemComp-PEG / DI(HYDROXYETHYL)ETHER


Mass: 106.120 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C4H10O3

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Details

Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.29 Å3/Da / Density % sol: 62.62 %
Crystal growTemperature: 277.15 K / Method: vapor diffusion, hanging drop / Details: 22% PEG 8000, 0.16M Li2SO4, 0.1M HEPES pH 7.6

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Australian Synchrotron / Beamline: MX2 / Wavelength: 0.95373 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Oct 11, 2023
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.95373 Å / Relative weight: 1
ReflectionResolution: 3.4→81.56 Å / Num. obs: 32890 / % possible obs: 99.72 % / Redundancy: 10.3 % / Biso Wilson estimate: 132.4 Å2 / Rpim(I) all: 0.07283 / Net I/σ(I): 14.52
Reflection shellResolution: 3.4→3.522 Å / Redundancy: 8.4 % / Mean I/σ(I) obs: 1.33 / Num. unique obs: 1716 / Rpim(I) all: 0.6499 / % possible all: 99.13

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Processing

Software
NameVersionClassification
PHENIX1.20.1_4487refinement
autoPROCdata reduction
Aimlessdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.4→81.56 Å / SU ML: 0.4954 / Cross valid method: FREE R-VALUE / σ(F): 1.91 / Phase error: 28.1828
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2531 3375 10.26 %
Rwork0.2059 29514 -
obs0.2106 32889 99.68 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 146.58 Å2
Refinement stepCycle: LAST / Resolution: 3.4→81.56 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4768 0 97 0 4865
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00244993
X-RAY DIFFRACTIONf_angle_d0.47346795
X-RAY DIFFRACTIONf_chiral_restr0.043739
X-RAY DIFFRACTIONf_plane_restr0.0058884
X-RAY DIFFRACTIONf_dihedral_angle_d11.75141847
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.4-3.450.41651380.37481214X-RAY DIFFRACTION98.4
3.45-3.50.36471510.33841210X-RAY DIFFRACTION98.55
3.5-3.550.35991260.321234X-RAY DIFFRACTION100
3.56-3.610.31831420.31631244X-RAY DIFFRACTION100
3.61-3.680.33421580.29461205X-RAY DIFFRACTION100
3.68-3.740.3011690.28821219X-RAY DIFFRACTION100
3.74-3.810.36751130.27841241X-RAY DIFFRACTION100
3.81-3.890.29331360.25831232X-RAY DIFFRACTION97.99
3.89-3.980.2891480.23131173X-RAY DIFFRACTION98.51
3.98-4.070.26711560.22891246X-RAY DIFFRACTION99.93
4.07-4.170.23421310.2011239X-RAY DIFFRACTION100
4.17-4.280.21531800.17641204X-RAY DIFFRACTION99.93
4.28-4.410.27941240.18471230X-RAY DIFFRACTION100
4.41-4.550.22881510.171230X-RAY DIFFRACTION100
4.55-4.710.20281420.15791223X-RAY DIFFRACTION100
4.72-4.90.23341320.16221275X-RAY DIFFRACTION100
4.91-5.130.26741260.16571227X-RAY DIFFRACTION100
5.13-5.40.20851390.17641248X-RAY DIFFRACTION100
5.4-5.740.24691390.19961234X-RAY DIFFRACTION100
5.74-6.180.25811350.2131240X-RAY DIFFRACTION100
6.18-6.80.26861490.22651219X-RAY DIFFRACTION99.93
6.8-7.780.20321220.19721249X-RAY DIFFRACTION100
7.78-9.80.20271510.16921241X-RAY DIFFRACTION100
9.81-81.560.28021170.20891237X-RAY DIFFRACTION99.19
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.616518665810.209487773946-3.412621813398.79504003137-0.5271092311045.389030965780.3094373954871.23853492703-0.7830286735691.407128340060.599230757466-1.009377793240.3834351565190.0452801741347-0.3666639091491.155978010240.17146229046-0.3808672549481.648503245630.233306058651.15810526976-30.085165972417.8246779796-14.72368862
23.173020213762.509889968772.067089402314.06743494154-0.1403979893682.950289964550.02595969736481.036997681850.08649981945681.250517421470.04095798949090.754383236215-0.813727622677-0.450845885682-0.4692159890751.339345782530.03396112796330.128276917850.9800482234610.2259026328340.792781889825-38.290040860612.0931471869-13.1298071164
32.70079421196-1.489371091012.184077569718.443840866373.716956985198.49340984152-0.437851587650.743610440441-0.6442465122410.06963751916311.02877180284-1.541709806261.58185631387-0.143715054581-0.4076166112171.53108635857-0.121362533657-0.3128060881871.51337045011-0.1075819708661.51580472764-30.76516075135.88606056624-21.8977112065
42.01252154631-4.38745611227-1.99656672278.660101339513.662289138725.1919021563-0.137247772539-2.014744140030.06642321170730.2470962078230.9398514973780.2185281141370.386334110154-0.981485781439-0.4617022878331.59062802991-0.230968865825-0.2055254589041.440277563680.5361177309951.45856517715-38.107571920310.263053378-1.83603131423
54.25208667593-0.544926620306-3.662200918463.99629059720.6792755817626.25021887797-0.6140455731140.150226156401-0.3666605398920.7773134786220.5767499423110.6175191624421.91370312846-0.5947094858990.03322985316730.746566328365-0.00913893115006-0.2008361910221.387861433380.1995883342061.27933509386-45.518597698822.1278547541-25.1659723498
64.10127493915-3.21099953735-1.524514611235.01505187454-1.646977610483.77701486473-0.116299354928-0.5559086346520.1275568580910.3453498838170.8073714725090.35070782873-0.235472466581-0.386709734596-0.5891086409830.558926706107-0.0554273026699-0.02612706522491.267100947950.2471676285081.01762230146-45.80160352417.6358102479-25.2815826578
77.226799603361.87522100559-0.91429237445.369968461041.167933230715.26459832445-0.7251900949830.548549047916-1.41348993542-0.8082833409310.5031460706281.68075425652-0.894469801596-1.05603641327-0.5781361300830.888640123029-0.0813843468858-0.3838234454551.46789848953-0.07043894593881.47949964798-47.692249213615.6698186926-38.1940640992
83.713352566310.39220404059-2.306969556793.312206562492.760488575156.59672916649-0.382948263371.292653268650.0442588779925-0.116154870929-0.7346046122931.56913029059-0.489498072785-3.47998784581.022133808410.752620531527-0.08249519581710.1005514939311.939861184710.1741996791831.73655724939-55.156983150421.151420438-23.850873679
90.998354472783-2.55128477054-2.190517590918.974327165445.203627652544.888395484051.61106908145-0.2648453645781.17827451135-2.957770911990.882340323062-0.549024416684-0.949043983581-0.15677293097-2.702431691581.51981985965-0.005245288402840.03438666305652.13438071270.06328870677611.51935586273-51.271129718925.0285117137-40.7794152182
104.653713509160.08247853559673.576445025963.802185819480.6617751296642.772876566650.0374394282047-0.5735948788960.08316651939950.9687824555330.327425428751-0.6850524043580.08510898488290.421996180133-0.2851947040221.130628242330.207403703641-0.2128437447061.26753835792-0.03399689772860.827149811444-27.087250419519.5838463802-8.00098675286
115.06138936897-0.0965042792864-1.396259904596.00249964135-1.151986098065.42242611944-0.0007065727020931.190264900680.31557334791-1.15491626990.00735870191439-0.320356890052-0.3026023504140.127137716081-0.07384745169360.7089785257190.01351670490670.04332418842111.65355752905-0.07775819403170.969899447998-31.783521618932.2431604841-44.2340079826
127.86085458199-2.97787293397-5.091688342146.05270764294.684820444344.84944422069-0.943495594322-2.29012373338-1.730323003320.655043477080.217223077161.70270502272.246120348271.044605628250.6509486138820.9190103349160.124750107307-0.2183931666980.9441799221780.2778318207330.954168131233-26.88405847179.06215402676-6.11888543101
132.011243255370.793038643536-1.81837663575.07411495597-2.592161014073.246805118840.9349208206470.9600493416391.70319540962-0.0132928413762-0.7067581138220.336955465664-0.5692298439361.21929201636-0.5029143234040.755002726767-0.1451832058620.1287894250971.280098705090.04707209115470.795531449745-48.512931924653.500303051-23.104487347
142.236642834681.959087380831.240993030972.74636408211-0.5410080361413.23745194485-0.201560675796-0.821820036511-0.96373636999-0.477390251266-0.638793893374-0.467229230506-0.4564477758680.220612927356-0.0939698685530.3794258668980.3761107792480.09275004838031.52906714706-0.1878211092710.951031235443-55.769479421839.3560915983-35.3384244652
152.79683612648-0.07242556896824.895073871543.131024067270.1516613374698.60437672383-0.1571012563750.207795319785-0.0004928592783730.176476619298-0.850561558137-0.321580174482-2.32137896939-0.296992859412-1.603375624840.9946678430170.161276664430.5642854563730.837774837750.09836629662540.994250796882-38.063895759152.512115659-35.5212294832
162.94081581382-1.478641833320.1120303282664.755216297371.873896018956.664746891070.34803803790.0815738360231-0.112981202303-0.537108846296-0.242730395367-0.293526016473-0.122123116368-0.0604808651717-0.2999373397470.3558765110950.0670791299142-0.004328993331481.08149108310.1074538440480.607680633483-47.620703113142.7599177086-29.8532416513
177.654160171570.190057736046-2.20402218235.568171007344.545245656256.8076183074-0.2064419899270.602373823814-0.7671549347911.53319784192-0.326972777224-0.4104782504431.287462301630.9367100875810.8138304218341.55570390196-0.086880746562-0.1017880770351.03700865730.3489848953131.09589650351-44.76367199765.5626054539-2.98374674384
184.65447537204-3.771044900724.167959353615.25076406838-1.295441733045.881852339550.326010465374-1.18788393065-1.860925485870.09834815464240.6075657199720.6011680052161.424738990310.150976717308-1.994487289731.92949472058-0.1586223664530.1377939877861.445789832840.1159195077231.35882681109-56.491478302256.59191925691.06987726986
194.336924390341.156165464792.68930311592.187465611154.321685710248.58168142178-0.976908445341-1.26654627397-1.587529691782.381829662830.803749557740.859089972374-0.3178483894020.5202622196390.4687026753821.84553273434-0.0521999241860.2935500150691.235765836790.213486645870.920532105086-55.187658676168.84444930951.85032315303
201.636906272510.6165562220041.451473661820.233542245470.5467154310131.289110097480.0645915378385-1.10187710879-0.9654898048640.870605024160.4186293609120.2836076430291.06931497464-0.539849574723-1.147112432352.809212702110.50779452890.4553258161081.174787488330.4259261573741.15084196723-49.107244326160.85512827469.69349369744
217.12401282223-0.68125453216-2.685422111523.390684747382.328469900682.462742528911.084765148270.908398720196-0.333345226016-0.2370602672660.0164958104620.855282323255-0.418781331228-0.675314496297-0.744269280430.778288505499-0.279065898962-0.06223600599990.5658938478510.08303287816730.724133027371-51.161851130358.1083598135-9.27813810548
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 3 through 14 )AA3 - 141 - 12
22chain 'A' and (resid 15 through 26 )AA15 - 2613 - 24
33chain 'A' and (resid 27 through 55 )AA27 - 5525 - 53
44chain 'A' and (resid 56 through 76 )AA56 - 7654 - 74
55chain 'A' and (resid 77 through 102 )AA77 - 10275 - 100
66chain 'A' and (resid 103 through 146 )AA103 - 146101 - 144
77chain 'A' and (resid 147 through 160 )AA147 - 160145 - 158
88chain 'A' and (resid 161 through 173 )AA161 - 173159 - 171
99chain 'A' and (resid 174 through 184 )AA174 - 184172 - 182
1010chain 'B' and (resid 0 through 97 )BB0 - 971 - 98
1111chain 'B' and (resid 98 through 190 )BB98 - 19099 - 191
1212chain 'G' and (resid 1 through 13 )GC1 - 131 - 13
1313chain 'D' and (resid 6 through 20 )DD6 - 201 - 15
1414chain 'D' and (resid 21 through 44 )DD21 - 4416 - 39
1515chain 'D' and (resid 45 through 82 )DD45 - 8240 - 57
1616chain 'D' and (resid 83 through 145 )DD83 - 14558 - 120
1717chain 'D' and (resid 146 through 176 )DD146 - 176121 - 151
1818chain 'D' and (resid 177 through 191 )DD177 - 191152 - 166
1919chain 'D' and (resid 192 through 206 )DD192 - 206167 - 181
2020chain 'D' and (resid 207 through 217 )DD207 - 217182 - 192
2121chain 'D' and (resid 218 through 235 )DD218 - 235193 - 210

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