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- PDB-9atu: Bifunctional Inhibition of Neutrophil Elastase by Eap4 from S. aureus -

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Basic information

Entry
Database: PDB / ID: 9atu
TitleBifunctional Inhibition of Neutrophil Elastase by Eap4 from S. aureus
Components
  • Extracellular Adherence Protein
  • Neutrophil elastase
KeywordsPROTEIN BINDING / PROTEASE INHIBITOR / IMMUNE EVASION
Function / homology
Function and homology information


leukocyte elastase / biosynthetic process of antibacterial peptides active against Gram-negative bacteria / neutrophil-mediated killing of fungus / negative regulation of chemotaxis / acute inflammatory response to antigenic stimulus / positive regulation of leukocyte tethering or rolling / response to yeast / leukocyte migration involved in inflammatory response / negative regulation of interleukin-8 production / negative regulation of chemokine production ...leukocyte elastase / biosynthetic process of antibacterial peptides active against Gram-negative bacteria / neutrophil-mediated killing of fungus / negative regulation of chemotaxis / acute inflammatory response to antigenic stimulus / positive regulation of leukocyte tethering or rolling / response to yeast / leukocyte migration involved in inflammatory response / negative regulation of interleukin-8 production / negative regulation of chemokine production / Antimicrobial peptides / cytokine binding / pyroptotic inflammatory response / Activation of Matrix Metalloproteinases / neutrophil-mediated killing of gram-negative bacterium / Collagen degradation / extracellular matrix disassembly / Pyroptosis / phagocytosis / response to UV / transcription repressor complex / phagocytic vesicle / Degradation of the extracellular matrix / secretory granule / Regulation of Complement cascade / positive regulation of interleukin-8 production / positive regulation of smooth muscle cell proliferation / positive regulation of MAP kinase activity / protein catabolic process / negative regulation of inflammatory response / intracellular calcium ion homeostasis / specific granule lumen / transcription corepressor activity / azurophil granule lumen / positive regulation of immune response / peptidase activity / heparin binding / collagen-containing extracellular matrix / endopeptidase activity / protease binding / response to lipopolysaccharide / defense response to bacterium / serine-type endopeptidase activity / Neutrophil degranulation / negative regulation of transcription by RNA polymerase II / cell surface / proteolysis / extracellular space / extracellular exosome / extracellular region / cytoplasm / cytosol
Similarity search - Function
MAP domain / MAP domain / MAP repeat profile. / Serine proteases, trypsin family, histidine active site / Serine proteases, trypsin family, serine active site / Peptidase S1A, chymotrypsin family / Serine proteases, trypsin family, histidine active site. / Serine proteases, trypsin domain profile. / Serine proteases, trypsin family, serine active site. / Trypsin-like serine protease ...MAP domain / MAP domain / MAP repeat profile. / Serine proteases, trypsin family, histidine active site / Serine proteases, trypsin family, serine active site / Peptidase S1A, chymotrypsin family / Serine proteases, trypsin family, histidine active site. / Serine proteases, trypsin domain profile. / Serine proteases, trypsin family, serine active site. / Trypsin-like serine protease / Serine proteases, trypsin domain / Trypsin / Peptidase S1, PA clan, chymotrypsin-like fold / Peptidase S1, PA clan
Similarity search - Domain/homology
Neutrophil elastase / Protein map
Similarity search - Component
Biological speciesStaphylococcus aureus subsp. aureus Mu50 (bacteria)
Homo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.05 Å
AuthorsMishra, N.B. / Herdendorf, T.J. / Geisbrecht, B.V.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35GM140852 United States
CitationJournal: To Be Published
Title: Bifunctional Inhibition of Neutrophil Elastase by Eap4 from S. aureus
Authors: Mishra, N.B. / Herdendorf, T.J. / Geisbrecht, B.V.
History
DepositionFeb 27, 2024Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jun 12, 2024Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Neutrophil elastase
B: Extracellular Adherence Protein
C: Neutrophil elastase
D: Neutrophil elastase
F: Neutrophil elastase
E: Extracellular Adherence Protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)123,34014
Polymers117,9496
Non-polymers5,3918
Water6,089338
1
C: Neutrophil elastase
F: Neutrophil elastase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)47,9414
Polymers46,6382
Non-polymers1,3032
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
D: Neutrophil elastase
F: Neutrophil elastase
E: Extracellular Adherence Protein
hetero molecules

A: Neutrophil elastase
B: Extracellular Adherence Protein
C: Neutrophil elastase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)123,34014
Polymers117,9496
Non-polymers5,3918
Water1086
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation2_565-x+1/2,-y+1,z+1/21
Buried area16230 Å2
ΔGint67 kcal/mol
Surface area45840 Å2
MethodPISA
3
A: Neutrophil elastase
B: Extracellular Adherence Protein
C: Neutrophil elastase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)61,5817
Polymers58,9743
Non-polymers2,6064
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area7520 Å2
ΔGint34 kcal/mol
Surface area23550 Å2
MethodPISA
4
A: Neutrophil elastase
B: Extracellular Adherence Protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)36,9594
Polymers35,6552
Non-polymers1,3032
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3870 Å2
ΔGint15 kcal/mol
Surface area15000 Å2
MethodPISA
5
D: Neutrophil elastase
F: Neutrophil elastase
E: Extracellular Adherence Protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)61,7597
Polymers58,9743
Non-polymers2,7854
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area7250 Å2
ΔGint25 kcal/mol
Surface area23740 Å2
MethodPISA
6
D: Neutrophil elastase
E: Extracellular Adherence Protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)36,9754
Polymers35,6552
Non-polymers1,3192
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3490 Å2
ΔGint9 kcal/mol
Surface area15240 Å2
MethodPISA
Unit cell
Length a, b, c (Å)75.816, 121.060, 147.628
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

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Protein , 2 types, 6 molecules ACDFBE

#1: Protein
Neutrophil elastase / Bone marrow serine protease / Elastase-2 / Human leukocyte elastase / HLE / Medullasin / PMN elastase


Mass: 23318.982 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / Cell: Neutrophil / References: UniProt: P08246, leukocyte elastase
#2: Protein Extracellular Adherence Protein / Protein map


Mass: 12336.466 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Staphylococcus aureus subsp. aureus Mu50 (bacteria)
Gene: map, SAV1938 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q99QS1

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Sugars , 3 types, 8 molecules

#3: Polysaccharide
beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1- ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 732.682 Da / Num. of mol.: 5
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpb1-4DGlcpNAcb1-4[LFucpa1-6]DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,4,3/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1221m-1a_1-5]/1-1-2-3/a4-b1_a6-d1_b4-c1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{}}[(6+1)][a-L-Fucp]{}}LINUCSPDB-CARE
#4: Polysaccharide 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta- ...2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 570.542 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4[LFucpa1-6]DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/2,3,2/[a2122h-1b_1-5_2*NCC/3=O][a1221m-1a_1-5]/1-1-2/a4-b1_a6-c1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}[(6+1)][a-L-Fucp]{}}LINUCSPDB-CARE
#5: Polysaccharide beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta- ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 586.542 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/2,3,2/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5]/1-1-2/a4-b1_b4-c1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{}}}LINUCSPDB-CARE

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Non-polymers , 1 types, 338 molecules

#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 338 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.87 Å3/Da / Density % sol: 57.17 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop
Details: 0.2 M ammonium acetate 0.1 M Tris-HCl (pH 8.0) 17% (w/v) PEG-10K

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Mar 2, 2023
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.05→50 Å / Num. obs: 85130 / % possible obs: 99.7 % / Redundancy: 12.8 % / CC1/2: 0.99 / CC star: 0.997 / Rmerge(I) obs: 0.103 / Rpim(I) all: 0.029 / Rrim(I) all: 0.107 / Χ2: 0.981 / Net I/σ(I): 8.6 / Num. measured all: 1088798
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. unique obsCC1/2CC starRpim(I) allRrim(I) allΧ2% possible all
2.05-2.129.81.38783740.60.8660.4421.4590.92999.3
2.12-2.2112.21.06484100.8270.9520.3121.1090.92399.9
2.21-2.3112.80.76484360.920.9790.220.7960.972100
2.31-2.4312.20.51684050.9460.9860.1530.5390.9799.2
2.43-2.58140.45384730.9830.9960.1230.471.059100
2.58-2.7813.90.2284820.9910.9980.060.2281.06999.9
2.78-3.0613.50.13785150.9950.9990.0380.1420.95199.9
3.06-3.5112.40.0984820.9960.9990.0270.0940.97999.1
3.51-4.42140.07286620.9970.9990.020.0750.983100
4.42-5012.90.06788910.9870.9970.020.070.94299.3

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Processing

Software
NameVersionClassification
PHENIX(1.20.1_4487: ???)refinement
HKL-2000data scaling
HKL-2000data reduction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.05→41.28 Å / SU ML: 0.27 / Cross valid method: FREE R-VALUE / σ(F): 0.24 / Phase error: 25.43 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2372 1877 2.35 %
Rwork0.1848 --
obs0.186 79893 93.56 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 2.05→41.28 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms8118 0 360 338 8816
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0088622
X-RAY DIFFRACTIONf_angle_d0.8811694
X-RAY DIFFRACTIONf_dihedral_angle_d13.3513217
X-RAY DIFFRACTIONf_chiral_restr0.0591439
X-RAY DIFFRACTIONf_plane_restr0.0081474
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.05-2.110.37641100.31854769X-RAY DIFFRACTION75
2.11-2.170.29751270.2645264X-RAY DIFFRACTION83
2.17-2.240.28221350.24175554X-RAY DIFFRACTION88
2.24-2.320.29191430.22235784X-RAY DIFFRACTION91
2.32-2.410.26461410.2075866X-RAY DIFFRACTION92
2.41-2.520.26211390.20676064X-RAY DIFFRACTION95
2.52-2.660.27331500.216169X-RAY DIFFRACTION97
2.66-2.820.27261510.22346266X-RAY DIFFRACTION98
2.82-3.040.27891550.21726330X-RAY DIFFRACTION99
3.04-3.350.2611540.20786378X-RAY DIFFRACTION99
3.35-3.830.22041540.17846357X-RAY DIFFRACTION99
3.83-4.830.18911570.1466529X-RAY DIFFRACTION100
4.83-41.280.22131610.15836686X-RAY DIFFRACTION99
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
15.26962.7487-0.56285.35310.42023.0927-0.20870.2109-0.27330.03440.2327-0.79580.67890.7325-0.06960.2880.0924-0.05910.2807-0.07630.407317.39542.406743.9447
23.27430.3382-0.11235.05650.34042.8127-0.0680.5183-0.0805-0.418-0.00470.09730.17060.01340.01310.27650.0066-0.04880.339-0.05290.28665.2097.43335.0532
32.25631.37070.33143.91630.46152.4779-0.07760.15920.23650.0548-0.06990.70160.1286-0.22060.09950.19810.0172-0.02030.313-0.05770.425-0.986710.241641.9619
44.01340.89160.88654.11120.47393.32010.1178-0.652-0.01160.8416-0.2434-0.0254-0.030.3302-0.03960.4936-0.0483-0.01170.4732-0.06230.370310.496712.659157.1252
53.6324-1.22641.57768.00681.03162.9893-0.12150.03810.28790.20290.3193-0.37970.040.5195-0.25160.1617-0.07380.01290.30240.02210.296517.655915.574645.9575
64.50482.0690.15442.83120.1111.49310.11510.01740.23190.2754-0.07620.1103-0.01270.0964-0.00150.29350.007-0.0380.2351-0.0420.356510.613216.150448.7967
76.1581-1.2712-0.877.80120.62236.96810.370.3925-0.2507-0.8465-0.5467-0.00520.36310.2820.10980.42650.10130.03950.36120.0150.29111.928635.358619.3593
85.025-5.7019-5.25317.91614.13827.82660.32671.1233-0.0991-3.112-0.1525-0.2357-2.08350.3312-0.61950.89590.04710.10650.60190.020.426915.329218.102624.4262
99.19696.4249-4.91144.9548-3.95015.1909-0.07170.2767-0.7866-0.1417-0.1034-1.029-0.17290.26610.17370.37130.08120.01250.4664-0.04230.35218.036733.227527.1513
108.48794.81781.90726.41350.58793.58950.1948-0.16160.3132-0.2268-0.1450.45760.0307-0.2115-0.05390.26380.0687-0.02860.31390.00480.326310.68535.458532.0477
111.6488-1.1707-0.94778.9724-2.841.98680.10480.1063-0.1365-0.1121-0.2035-0.38320.00110.30670.09170.2308-0.008-0.03020.35910.00810.300613.029826.050736.6371
128.4542.92791.43717.2294-0.7043.56370.22220.15950.5177-0.7355-0.21281.49030.3181-0.8232-0.15520.53030.0141-0.01360.45370.0050.31077.47332.06925.2229
132.24550.0319-0.24172.88720.57914.64440.00630.8002-0.0735-0.64180.1821-0.17320.17870.4277-0.20760.7160.05450.07840.6552-0.01140.372913.515445.73532.426
149.3563-2.5084.08174.55810.92133.36890.3855-0.2312-0.882-0.6135-0.05861.65711.5478-0.0236-0.35040.824-0.0931-0.06310.52740.00280.5554-0.981238.34318.5733
153.58490.3242-1.38792.27821.07964.54630.0710.40680.358-0.39150.1506-0.0837-0.36330.2432-0.2140.54930.01820.01480.43530.07950.39917.8754.403111.4521
165.47840.47490.76825.18430.34961.41550.4507-0.4385-0.79830.1844-0.3829-1.3480.14450.53370.01090.3039-0.0367-0.04820.56270.07210.741749.816648.320548.8614
176.04990.10830.45555.4201-0.39583.02530.0485-0.75350.19360.7025-0.2326-0.3335-0.32740.33720.10320.473-0.1210.01040.54070.01120.452939.565959.474553.9201
185.38041.66991.66723.73531.9273.18030.0894-0.16180.5971-0.254-0.26690.3826-0.5724-0.19240.23760.3661-0.00790.0490.38120.03010.536729.999761.790544.8746
195.96221.15580.85743.44951.67563.07020.21860.24650.68840.0623-0.1151-0.3686-0.09920.8363-0.23490.5092-0.16540.08160.49480.09020.538645.591865.202546.241
200.8073-0.4466-0.46915.7708-1.11271.39030.030.6699-0.2041-0.8188-0.4279-0.74230.14820.66890.53180.4740.00340.10950.7815-0.00010.520541.405149.454232.939
215.88924.2395-0.66359.1171-2.05065.04780.6768-0.5607-0.75220.884-0.6507-0.5380.61250.5763-0.02840.6313-0.1257-0.15970.65920.20130.672341.083142.713256.4187
225.08461.75060.53285.451.00883.48850.2440.6734-0.4654-0.35960.0716-0.65610.09820.3413-0.0530.40470.06350.0830.4409-0.04580.458236.299443.61236.583
235.24712.0270.40996.92140.20884.85370.2673-0.1427-1.0230.096-0.2921-1.00520.5213-0.018-0.06180.3884-0.007-0.00740.51550.06090.677637.662241.230846.1386
245.26242.76490.75396.63690.34153.5690.05430.3729-0.3885-0.1245-0.0719-0.43590.02960.23810.03260.27490.0580.00790.3717-0.00950.433536.124645.529640.5443
254.65271.01950.71667.9334-0.46925.0191-0.09790.40250.5182-1.3398-0.12690.2871-0.56840.55510.1390.4576-0.06280.04780.47270.0680.39335.403360.474135.781
263.9421-0.5146-0.64723.27270.28264.9513-0.1085-0.2747-0.02490.48910.230.5138-0.31650.109-0.13210.47820.00630.23890.36450.0180.5681-0.855645.532180.7075
274.6227-0.48095.36193.08452.41349.5894-0.14480.66771.1298-0.3980.22190.6582-1.67321.3156-0.02230.8033-0.05970.17420.60060.09330.78532.814759.596771.5078
284.25320.3301-1.25332.9061-0.06085.6964-0.00090.59250.0555-0.18830.09740.8995-0.2292-0.8055-0.06710.43170.07280.08190.51330.00820.7658-8.871545.35369.5469
290.8072.34710.22948.41591.64332.40320.0068-0.04570.21070.0990.4206-0.4518-0.5640.5278-0.38840.4022-0.0890.11820.5445-0.15050.44314.945947.989365.7559
308.011-0.06170.12155.80672.2498.4903-0.0893-0.5273-0.1691-0.15040.24670.345-0.4292-0.2069-0.18370.4184-0.05730.10890.3880.03830.4329.270345.949465.5034
317.14154.4208-3.87176.9736-1.62532.23620.0744-2.73590.26731.1064-0.8071-0.1470.08291.58770.79750.7561-0.14770.02711.2528-0.08860.539530.270748.364264.3905
325.37366.3169-6.14538.3288-6.7579.24510.2762-0.4017-0.17580.7861-0.4225-0.1834-0.44640.19650.10420.3876-0.01850.01770.35210.00150.38217.441740.647259.9598
336.4114.8379-0.03387.50640.23682.90030.05040.1550.4108-0.3295-0.05120.5966-0.15950.02080.04030.29620.01190.05390.2318-0.00430.263414.279945.91553.0378
348.42582.7053-2.02765.94440.82573.04860.1971-0.47250.03090.0154-0.17370.0642-0.11450.16980.0190.3482-0.01250.03080.32490.07590.245219.861548.538754.9953
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 29 through 42 )
2X-RAY DIFFRACTION2chain 'A' and (resid 43 through 95 )
3X-RAY DIFFRACTION3chain 'A' and (resid 96 through 137 )
4X-RAY DIFFRACTION4chain 'A' and (resid 138 through 154 )
5X-RAY DIFFRACTION5chain 'A' and (resid 155 through 188 )
6X-RAY DIFFRACTION6chain 'A' and (resid 189 through 246 )
7X-RAY DIFFRACTION7chain 'B' and (resid 371 through 393 )
8X-RAY DIFFRACTION8chain 'B' and (resid 394 through 400 )
9X-RAY DIFFRACTION9chain 'B' and (resid 401 through 416 )
10X-RAY DIFFRACTION10chain 'B' and (resid 417 through 444 )
11X-RAY DIFFRACTION11chain 'B' and (resid 445 through 456 )
12X-RAY DIFFRACTION12chain 'B' and (resid 457 through 468 )
13X-RAY DIFFRACTION13chain 'C' and (resid 29 through 154 )
14X-RAY DIFFRACTION14chain 'C' and (resid 155 through 168 )
15X-RAY DIFFRACTION15chain 'C' and (resid 169 through 246 )
16X-RAY DIFFRACTION16chain 'D' and (resid 29 through 42 )
17X-RAY DIFFRACTION17chain 'D' and (resid 43 through 95 )
18X-RAY DIFFRACTION18chain 'D' and (resid 96 through 117 )
19X-RAY DIFFRACTION19chain 'D' and (resid 118 through 137 )
20X-RAY DIFFRACTION20chain 'D' and (resid 138 through 154 )
21X-RAY DIFFRACTION21chain 'D' and (resid 155 through 168 )
22X-RAY DIFFRACTION22chain 'D' and (resid 169 through 188 )
23X-RAY DIFFRACTION23chain 'D' and (resid 189 through 203 )
24X-RAY DIFFRACTION24chain 'D' and (resid 204 through 228 )
25X-RAY DIFFRACTION25chain 'D' and (resid 229 through 246 )
26X-RAY DIFFRACTION26chain 'F' and (resid 29 through 154 )
27X-RAY DIFFRACTION27chain 'F' and (resid 155 through 168 )
28X-RAY DIFFRACTION28chain 'F' and (resid 169 through 246 )
29X-RAY DIFFRACTION29chain 'E' and (resid 371 through 380 )
30X-RAY DIFFRACTION30chain 'E' and (resid 381 through 393 )
31X-RAY DIFFRACTION31chain 'E' and (resid 394 through 400 )
32X-RAY DIFFRACTION32chain 'E' and (resid 401 through 416 )
33X-RAY DIFFRACTION33chain 'E' and (resid 417 through 444 )
34X-RAY DIFFRACTION34chain 'E' and (resid 445 through 468 )

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