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Yorodumi- PDB-8zyg: Crystal structure of a cupin protein (tm1459, I49C-4py/H52A/C106D... -
+Open data
-Basic information
Entry | Database: PDB / ID: 8zyg | |||||||||||||||
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Title | Crystal structure of a cupin protein (tm1459, I49C-4py/H52A/C106D mutant) in copper (Cu) substituted form | |||||||||||||||
Components | Cupin type-2 domain-containing protein | |||||||||||||||
Keywords | METAL BINDING PROTEIN / Artificial Metalloenzymes / Chemical Modification | |||||||||||||||
Function / homology | Cupin 2, conserved barrel / Cupin domain / RmlC-like cupin domain superfamily / RmlC-like jelly roll fold / metal ion binding / COPPER (II) ION / Cupin type-2 domain-containing protein Function and homology information | |||||||||||||||
Biological species | Thermotoga maritima MSB8 (bacteria) | |||||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.08 Å | |||||||||||||||
Authors | Morita, Y. / Kubo, H. / Matsumoto, R. / Fujieda, N. | |||||||||||||||
Funding support | Japan, 4items
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Citation | Journal: To Be Published Title: Copper center in artificial non-heme metalloenzyme Authors: Morita, Y. / Kubo, H. / Matsumoto, R. / Fujieda, N. | |||||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8zyg.cif.gz | 161.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8zyg.ent.gz | 127 KB | Display | PDB format |
PDBx/mmJSON format | 8zyg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8zyg_validation.pdf.gz | 443.4 KB | Display | wwPDB validaton report |
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Full document | 8zyg_full_validation.pdf.gz | 443.5 KB | Display | |
Data in XML | 8zyg_validation.xml.gz | 15.9 KB | Display | |
Data in CIF | 8zyg_validation.cif.gz | 21.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zy/8zyg ftp://data.pdbj.org/pub/pdb/validation_reports/zy/8zyg | HTTPS FTP |
-Related structure data
Related structure data | 8zyhC C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 13394.295 Da / Num. of mol.: 2 / Mutation: I49C/H52A/C106D Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermotoga maritima MSB8 (bacteria) / Gene: TM_1459 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q9X1H0 #2: Chemical | ChemComp-P6G / | #3: Chemical | #4: Chemical | ChemComp-MES / | #5: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.05 Å3/Da / Density % sol: 40.09 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: 26-28% w/v Jeffamine ED-2001, 0.1M MES pH6.0 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL44XU / Wavelength: 0.9 Å |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jan 31, 2024 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 |
Reflection | Resolution: 1.08→50 Å / Num. obs: 184425 / % possible obs: 99.1 % / Redundancy: 3.35 % / CC1/2: 0.993 / Rmerge(I) obs: 0.075 / Net I/σ(I): 9.77 |
Reflection shell | Resolution: 1.08→1.14 Å / Redundancy: 3.39 % / Rmerge(I) obs: 0.804 / Mean I/σ(I) obs: 2.13 / Num. unique obs: 29487 / CC1/2: 0.711 / % possible all: 97.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.08→50 Å / Cross valid method: FREE R-VALUE / σ(F): 0 / Stereochemistry target values: ENGH AND HUBER Details: ANISOTROPIC REFINEMENT REDUCED FREE R (NO CUTOFF) BY ?
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Refine analyze | Num. disordered residues: 29 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.08→50 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.08→1.12 Å
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