C: F97M variant of the biphotochromic fluorescent protein moxSAASoti A: F97M variant of the biphotochromic fluorescent protein moxSAASoti hetero molecules
Mass: 25177.527 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Stylocoeniella armata (invertebrata) / Production host: Escherichia coli BL21(DE3) (bacteria)
Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2→21.83 Å / Cor.coef. Fo:Fc: 0.958 / Cor.coef. Fo:Fc free: 0.929 / SU B: 7.616 / SU ML: 0.111 / Cross valid method: THROUGHOUT / ESU R: 0.192 / ESU R Free: 0.172 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.23107
1475
5 %
RANDOM
Rwork
0.17764
-
-
-
obs
0.18041
27773
97.78 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK